Structure of the unusual Sinorhizobium fredii HH103 lipopolysaccharide and its role in symbiosis

Autores
Di Lorenzo, Flaviana; Speciale, Immacolata; Silipo, Alba; Alías Villegas, Cynthia; Acosta Jurado, Sebastián; Rodríguez Carvajal, Miguel Ángel; Dardanelli, Marta Susana; Palmigiano, Angelo; Garozzo, Domenico; Ruiz Sainz, José Enrique; Molinaro, Antonio; Vinardell, José María
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Rhizobia are soil bacteria that form important symbiotic associations with legumes, and rhizobial surface polysaccharides, such as K-antigen polysaccharide (KPS) and lipopolysaccharide (LPS), might be important for symbiosis. Previously, we obtained a mutant of Sinorhizobium fredii HH103, rkpA, that does not produce KPS, a homopolysaccharide of a pseudaminic acid derivative, but whose LPS electrophoretic profile was indistinguishable from that of the WT strain. We also previously demonstrated that the HH103 rkpLMNOPQ operon is responsible for 5-acetamido-3,5,7,9-tetradeoxy-7-(3-hydroxybutyramido)-L-glyc-ero-L-manno-nonulosonic acid [Pse5NAc7(3OHBu)] production and is involved in HH103 KPS and LPS biosynthesis and that an HH103 rkpM mutant cannot produce KPS and displays an altered LPS structure. Here, we analyzed the LPS structure of HH103 rkpA, focusing on the carbohydrate portion, and found that it contains a highly heterogeneous lipid A and a peculiar core oligosaccharide composed of an unusually high number of hexuronic acids containing b-configured Pse5NAc7(3OHBu). This pseudaminic acid derivative, in its a-configuration, was the only structural component of the S. fredii HH103 KPS and, to the best of our knowledge, has never been reported from any other rhizobial LPS. We also show that Pse5NAc7(3OHBu) is the complete or partial epitope for a mAb, NB6-228.22, that can recognize the HH103 LPS, but not those of most of the S. fredii strains tested here. We also show that the LPS from HH103 rkpM is identical to that of HH103 rkpA but devoid of any Pse5NAc7(3OHBu) residues. Notably, this rkpM mutant was severely impaired in symbiosis with its host, Macroptilium atropurpureum.
Fil: Di Lorenzo, Flaviana. Università degli Studi di Napoli Federico II; Italia
Fil: Speciale, Immacolata. Università degli Studi di Napoli Federico II; Italia
Fil: Silipo, Alba. Università degli Studi di Napoli Federico II; Italia
Fil: Alías Villegas, Cynthia. Universidad de Sevilla; España
Fil: Acosta Jurado, Sebastián. Universidad de Sevilla; España
Fil: Rodríguez Carvajal, Miguel Ángel. Universidad de Sevilla; España
Fil: Dardanelli, Marta Susana. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Biotecnología Ambiental y Salud - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Biotecnología Ambiental y Salud; Argentina
Fil: Palmigiano, Angelo. Consiglio Nazionale delle Ricerche; Italia
Fil: Garozzo, Domenico. Consiglio Nazionale delle Ricerche; Italia
Fil: Ruiz Sainz, José Enrique. Universidad de Sevilla; España
Fil: Molinaro, Antonio. University of Naples Federico II; Italia
Fil: Vinardell, José María. Universidad de Sevilla; España
Materia
Sinorhizobium
lipopolysaccharide
soybean
antibody
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/145194
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/145194
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Structure of the unusual Sinorhizobium fredii HH103 lipopolysaccharide and its role in symbiosisDi Lorenzo, FlavianaSpeciale, ImmacolataSilipo, AlbaAlías Villegas, CynthiaAcosta Jurado, SebastiánRodríguez Carvajal, Miguel ÁngelDardanelli, Marta SusanaPalmigiano, AngeloGarozzo, DomenicoRuiz Sainz, José EnriqueMolinaro, AntonioVinardell, José MaríaSinorhizobiumlipopolysaccharidesoybeanantibodyhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Rhizobia are soil bacteria that form important symbiotic associations with legumes, and rhizobial surface polysaccharides, such as K-antigen polysaccharide (KPS) and lipopolysaccharide (LPS), might be important for symbiosis. Previously, we obtained a mutant of Sinorhizobium fredii HH103, rkpA, that does not produce KPS, a homopolysaccharide of a pseudaminic acid derivative, but whose LPS electrophoretic profile was indistinguishable from that of the WT strain. We also previously demonstrated that the HH103 rkpLMNOPQ operon is responsible for 5-acetamido-3,5,7,9-tetradeoxy-7-(3-hydroxybutyramido)-L-glyc-ero-L-manno-nonulosonic acid [Pse5NAc7(3OHBu)] production and is involved in HH103 KPS and LPS biosynthesis and that an HH103 rkpM mutant cannot produce KPS and displays an altered LPS structure. Here, we analyzed the LPS structure of HH103 rkpA, focusing on the carbohydrate portion, and found that it contains a highly heterogeneous lipid A and a peculiar core oligosaccharide composed of an unusually high number of hexuronic acids containing b-configured Pse5NAc7(3OHBu). This pseudaminic acid derivative, in its a-configuration, was the only structural component of the S. fredii HH103 KPS and, to the best of our knowledge, has never been reported from any other rhizobial LPS. We also show that Pse5NAc7(3OHBu) is the complete or partial epitope for a mAb, NB6-228.22, that can recognize the HH103 LPS, but not those of most of the S. fredii strains tested here. We also show that the LPS from HH103 rkpM is identical to that of HH103 rkpA but devoid of any Pse5NAc7(3OHBu) residues. Notably, this rkpM mutant was severely impaired in symbiosis with its host, Macroptilium atropurpureum.Fil: Di Lorenzo, Flaviana. Università degli Studi di Napoli Federico II; ItaliaFil: Speciale, Immacolata. Università degli Studi di Napoli Federico II; ItaliaFil: Silipo, Alba. Università degli Studi di Napoli Federico II; ItaliaFil: Alías Villegas, Cynthia. Universidad de Sevilla; EspañaFil: Acosta Jurado, Sebastián. Universidad de Sevilla; EspañaFil: Rodríguez Carvajal, Miguel Ángel. Universidad de Sevilla; EspañaFil: Dardanelli, Marta Susana. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Biotecnología Ambiental y Salud - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Biotecnología Ambiental y Salud; ArgentinaFil: Palmigiano, Angelo. Consiglio Nazionale delle Ricerche; ItaliaFil: Garozzo, Domenico. Consiglio Nazionale delle Ricerche; ItaliaFil: Ruiz Sainz, José Enrique. Universidad de Sevilla; EspañaFil: Molinaro, Antonio. University of Naples Federico II; ItaliaFil: Vinardell, José María. Universidad de Sevilla; EspañaAmerican Society for Biochemistry and Molecular Biology2020-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/145194Di Lorenzo, Flaviana; Speciale, Immacolata; Silipo, Alba; Alías Villegas, Cynthia; Acosta Jurado, Sebastián; et al.; Structure of the unusual Sinorhizobium fredii HH103 lipopolysaccharide and its role in symbiosis; American Society for Biochemistry and Molecular Biology; Journal of Biological Chemistry (online); 295; 32; 8-2020; 10969-109870021-9258CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.jbc.org/lookup/doi/10.1074/jbc.RA120.013393info:eu-repo/semantics/altIdentifier/doi/10.1074/jbc.RA120.013393info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:11:03Zoai:ri.conicet.gov.ar:11336/145194instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:11:04.076CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Structure of the unusual Sinorhizobium fredii HH103 lipopolysaccharide and its role in symbiosis
title Structure of the unusual Sinorhizobium fredii HH103 lipopolysaccharide and its role in symbiosis
spellingShingle Structure of the unusual Sinorhizobium fredii HH103 lipopolysaccharide and its role in symbiosis
Di Lorenzo, Flaviana
Sinorhizobium
lipopolysaccharide
soybean
antibody
title_short Structure of the unusual Sinorhizobium fredii HH103 lipopolysaccharide and its role in symbiosis
title_full Structure of the unusual Sinorhizobium fredii HH103 lipopolysaccharide and its role in symbiosis
title_fullStr Structure of the unusual Sinorhizobium fredii HH103 lipopolysaccharide and its role in symbiosis
title_full_unstemmed Structure of the unusual Sinorhizobium fredii HH103 lipopolysaccharide and its role in symbiosis
title_sort Structure of the unusual Sinorhizobium fredii HH103 lipopolysaccharide and its role in symbiosis
dc.creator.none.fl_str_mv Di Lorenzo, Flaviana
Speciale, Immacolata
Silipo, Alba
Alías Villegas, Cynthia
Acosta Jurado, Sebastián
Rodríguez Carvajal, Miguel Ángel
Dardanelli, Marta Susana
Palmigiano, Angelo
Garozzo, Domenico
Ruiz Sainz, José Enrique
Molinaro, Antonio
Vinardell, José María
author Di Lorenzo, Flaviana
author_facet Di Lorenzo, Flaviana
Speciale, Immacolata
Silipo, Alba
Alías Villegas, Cynthia
Acosta Jurado, Sebastián
Rodríguez Carvajal, Miguel Ángel
Dardanelli, Marta Susana
Palmigiano, Angelo
Garozzo, Domenico
Ruiz Sainz, José Enrique
Molinaro, Antonio
Vinardell, José María
author_role author
author2 Speciale, Immacolata
Silipo, Alba
Alías Villegas, Cynthia
Acosta Jurado, Sebastián
Rodríguez Carvajal, Miguel Ángel
Dardanelli, Marta Susana
Palmigiano, Angelo
Garozzo, Domenico
Ruiz Sainz, José Enrique
Molinaro, Antonio
Vinardell, José María
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Sinorhizobium
lipopolysaccharide
soybean
antibody
topic Sinorhizobium
lipopolysaccharide
soybean
antibody
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Rhizobia are soil bacteria that form important symbiotic associations with legumes, and rhizobial surface polysaccharides, such as K-antigen polysaccharide (KPS) and lipopolysaccharide (LPS), might be important for symbiosis. Previously, we obtained a mutant of Sinorhizobium fredii HH103, rkpA, that does not produce KPS, a homopolysaccharide of a pseudaminic acid derivative, but whose LPS electrophoretic profile was indistinguishable from that of the WT strain. We also previously demonstrated that the HH103 rkpLMNOPQ operon is responsible for 5-acetamido-3,5,7,9-tetradeoxy-7-(3-hydroxybutyramido)-L-glyc-ero-L-manno-nonulosonic acid [Pse5NAc7(3OHBu)] production and is involved in HH103 KPS and LPS biosynthesis and that an HH103 rkpM mutant cannot produce KPS and displays an altered LPS structure. Here, we analyzed the LPS structure of HH103 rkpA, focusing on the carbohydrate portion, and found that it contains a highly heterogeneous lipid A and a peculiar core oligosaccharide composed of an unusually high number of hexuronic acids containing b-configured Pse5NAc7(3OHBu). This pseudaminic acid derivative, in its a-configuration, was the only structural component of the S. fredii HH103 KPS and, to the best of our knowledge, has never been reported from any other rhizobial LPS. We also show that Pse5NAc7(3OHBu) is the complete or partial epitope for a mAb, NB6-228.22, that can recognize the HH103 LPS, but not those of most of the S. fredii strains tested here. We also show that the LPS from HH103 rkpM is identical to that of HH103 rkpA but devoid of any Pse5NAc7(3OHBu) residues. Notably, this rkpM mutant was severely impaired in symbiosis with its host, Macroptilium atropurpureum.
Fil: Di Lorenzo, Flaviana. Università degli Studi di Napoli Federico II; Italia
Fil: Speciale, Immacolata. Università degli Studi di Napoli Federico II; Italia
Fil: Silipo, Alba. Università degli Studi di Napoli Federico II; Italia
Fil: Alías Villegas, Cynthia. Universidad de Sevilla; España
Fil: Acosta Jurado, Sebastián. Universidad de Sevilla; España
Fil: Rodríguez Carvajal, Miguel Ángel. Universidad de Sevilla; España
Fil: Dardanelli, Marta Susana. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Biotecnología Ambiental y Salud - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Biotecnología Ambiental y Salud; Argentina
Fil: Palmigiano, Angelo. Consiglio Nazionale delle Ricerche; Italia
Fil: Garozzo, Domenico. Consiglio Nazionale delle Ricerche; Italia
Fil: Ruiz Sainz, José Enrique. Universidad de Sevilla; España
Fil: Molinaro, Antonio. University of Naples Federico II; Italia
Fil: Vinardell, José María. Universidad de Sevilla; España
description Rhizobia are soil bacteria that form important symbiotic associations with legumes, and rhizobial surface polysaccharides, such as K-antigen polysaccharide (KPS) and lipopolysaccharide (LPS), might be important for symbiosis. Previously, we obtained a mutant of Sinorhizobium fredii HH103, rkpA, that does not produce KPS, a homopolysaccharide of a pseudaminic acid derivative, but whose LPS electrophoretic profile was indistinguishable from that of the WT strain. We also previously demonstrated that the HH103 rkpLMNOPQ operon is responsible for 5-acetamido-3,5,7,9-tetradeoxy-7-(3-hydroxybutyramido)-L-glyc-ero-L-manno-nonulosonic acid [Pse5NAc7(3OHBu)] production and is involved in HH103 KPS and LPS biosynthesis and that an HH103 rkpM mutant cannot produce KPS and displays an altered LPS structure. Here, we analyzed the LPS structure of HH103 rkpA, focusing on the carbohydrate portion, and found that it contains a highly heterogeneous lipid A and a peculiar core oligosaccharide composed of an unusually high number of hexuronic acids containing b-configured Pse5NAc7(3OHBu). This pseudaminic acid derivative, in its a-configuration, was the only structural component of the S. fredii HH103 KPS and, to the best of our knowledge, has never been reported from any other rhizobial LPS. We also show that Pse5NAc7(3OHBu) is the complete or partial epitope for a mAb, NB6-228.22, that can recognize the HH103 LPS, but not those of most of the S. fredii strains tested here. We also show that the LPS from HH103 rkpM is identical to that of HH103 rkpA but devoid of any Pse5NAc7(3OHBu) residues. Notably, this rkpM mutant was severely impaired in symbiosis with its host, Macroptilium atropurpureum.
publishDate 2020
dc.date.none.fl_str_mv 2020-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/145194
Di Lorenzo, Flaviana; Speciale, Immacolata; Silipo, Alba; Alías Villegas, Cynthia; Acosta Jurado, Sebastián; et al.; Structure of the unusual Sinorhizobium fredii HH103 lipopolysaccharide and its role in symbiosis; American Society for Biochemistry and Molecular Biology; Journal of Biological Chemistry (online); 295; 32; 8-2020; 10969-10987
0021-9258
CONICET Digital
CONICET
url http://hdl.handle.net/11336/145194
identifier_str_mv Di Lorenzo, Flaviana; Speciale, Immacolata; Silipo, Alba; Alías Villegas, Cynthia; Acosta Jurado, Sebastián; et al.; Structure of the unusual Sinorhizobium fredii HH103 lipopolysaccharide and its role in symbiosis; American Society for Biochemistry and Molecular Biology; Journal of Biological Chemistry (online); 295; 32; 8-2020; 10969-10987
0021-9258
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.jbc.org/lookup/doi/10.1074/jbc.RA120.013393
info:eu-repo/semantics/altIdentifier/doi/10.1074/jbc.RA120.013393
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Society for Biochemistry and Molecular Biology
publisher.none.fl_str_mv American Society for Biochemistry and Molecular Biology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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