Identification of fast and slow growing rhizobia nodulating soybean (Glycine max [L.] Merr) by a multiplex PCR reaction
- Autores
- Pastorino, Graciela Noemí; Martínez Alcántara, Virginia; Balatti, Pedro Alberto
- Año de publicación
- 2003
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Two DNA fragments, a 730-bp and a 900-bp fragment, one homologous to host cultivar specificity genes nolBT of Sinorhizobium fredii and the other one homologous to RSα, an insertion-like sequence present in Bradyrhizobium japonicum, were generated by polymerase chain reaction (PCR) with two pairs of primers. The amount of each fragment generated by the multiplex PCR was proportional to the amount of template DNA present. The amplification of the 900-bp RSα fragment was more sensitive, since it was amplified from a smaller amount of template DNA than the 730-bp nolBT fragment. By running the multiplex reaction in the presence of template DNA isolated from different sources, we confirmed that the reaction can discriminate between S. fredii, Bradyrhizobium japonicum and Sinorhizobium xinjiangensis.
Facultad de Ciencias Agrarias y Forestales
Instituto de Fisiología Vegetal - Materia
-
Ciencias Agrarias
Bradyrhizobium
Molecular marker
Polymerase chain reaction
Rhizobium
Sinorhizobium
Soybean - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by-nc-sa/4.0/
- Repositorio
.jpg)
- Institución
- Universidad Nacional de La Plata
- OAI Identificador
- oai:sedici.unlp.edu.ar:10915/84343
Ver los metadatos del registro completo
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Identification of fast and slow growing rhizobia nodulating soybean (Glycine max [L.] Merr) by a multiplex PCR reactionPastorino, Graciela NoemíMartínez Alcántara, VirginiaBalatti, Pedro AlbertoCiencias AgrariasBradyrhizobiumMolecular markerPolymerase chain reactionRhizobiumSinorhizobiumSoybeanTwo DNA fragments, a 730-bp and a 900-bp fragment, one homologous to host cultivar specificity genes <i>nolBT</i> of <i>Sinorhizobium fredii</i> and the other one homologous to RSα, an insertion-like sequence present in <i>Bradyrhizobium japonicum</i>, were generated by polymerase chain reaction (PCR) with two pairs of primers. The amount of each fragment generated by the multiplex PCR was proportional to the amount of template DNA present. The amplification of the 900-bp RSα fragment was more sensitive, since it was amplified from a smaller amount of template DNA than the 730-bp <i>nolBT</i> fragment. By running the multiplex reaction in the presence of template DNA isolated from different sources, we confirmed that the reaction can discriminate between <i>S. fredii</i>, <i>Bradyrhizobium japonicum</i> and <i>Sinorhizobium xinjiangensis</i>.Facultad de Ciencias Agrarias y ForestalesInstituto de Fisiología Vegetal2003info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf153-158http://sedici.unlp.edu.ar/handle/10915/84343enginfo:eu-repo/semantics/altIdentifier/issn/0378-1097info:eu-repo/semantics/altIdentifier/doi/10.1016/S0378-1097(03)00796-1info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-12-23T11:18:27Zoai:sedici.unlp.edu.ar:10915/84343Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-12-23 11:18:28.191SEDICI (UNLP) - Universidad Nacional de La Platafalse |
| dc.title.none.fl_str_mv |
Identification of fast and slow growing rhizobia nodulating soybean (Glycine max [L.] Merr) by a multiplex PCR reaction |
| title |
Identification of fast and slow growing rhizobia nodulating soybean (Glycine max [L.] Merr) by a multiplex PCR reaction |
| spellingShingle |
Identification of fast and slow growing rhizobia nodulating soybean (Glycine max [L.] Merr) by a multiplex PCR reaction Pastorino, Graciela Noemí Ciencias Agrarias Bradyrhizobium Molecular marker Polymerase chain reaction Rhizobium Sinorhizobium Soybean |
| title_short |
Identification of fast and slow growing rhizobia nodulating soybean (Glycine max [L.] Merr) by a multiplex PCR reaction |
| title_full |
Identification of fast and slow growing rhizobia nodulating soybean (Glycine max [L.] Merr) by a multiplex PCR reaction |
| title_fullStr |
Identification of fast and slow growing rhizobia nodulating soybean (Glycine max [L.] Merr) by a multiplex PCR reaction |
| title_full_unstemmed |
Identification of fast and slow growing rhizobia nodulating soybean (Glycine max [L.] Merr) by a multiplex PCR reaction |
| title_sort |
Identification of fast and slow growing rhizobia nodulating soybean (Glycine max [L.] Merr) by a multiplex PCR reaction |
| dc.creator.none.fl_str_mv |
Pastorino, Graciela Noemí Martínez Alcántara, Virginia Balatti, Pedro Alberto |
| author |
Pastorino, Graciela Noemí |
| author_facet |
Pastorino, Graciela Noemí Martínez Alcántara, Virginia Balatti, Pedro Alberto |
| author_role |
author |
| author2 |
Martínez Alcántara, Virginia Balatti, Pedro Alberto |
| author2_role |
author author |
| dc.subject.none.fl_str_mv |
Ciencias Agrarias Bradyrhizobium Molecular marker Polymerase chain reaction Rhizobium Sinorhizobium Soybean |
| topic |
Ciencias Agrarias Bradyrhizobium Molecular marker Polymerase chain reaction Rhizobium Sinorhizobium Soybean |
| dc.description.none.fl_txt_mv |
Two DNA fragments, a 730-bp and a 900-bp fragment, one homologous to host cultivar specificity genes <i>nolBT</i> of <i>Sinorhizobium fredii</i> and the other one homologous to RSα, an insertion-like sequence present in <i>Bradyrhizobium japonicum</i>, were generated by polymerase chain reaction (PCR) with two pairs of primers. The amount of each fragment generated by the multiplex PCR was proportional to the amount of template DNA present. The amplification of the 900-bp RSα fragment was more sensitive, since it was amplified from a smaller amount of template DNA than the 730-bp <i>nolBT</i> fragment. By running the multiplex reaction in the presence of template DNA isolated from different sources, we confirmed that the reaction can discriminate between <i>S. fredii</i>, <i>Bradyrhizobium japonicum</i> and <i>Sinorhizobium xinjiangensis</i>. Facultad de Ciencias Agrarias y Forestales Instituto de Fisiología Vegetal |
| description |
Two DNA fragments, a 730-bp and a 900-bp fragment, one homologous to host cultivar specificity genes <i>nolBT</i> of <i>Sinorhizobium fredii</i> and the other one homologous to RSα, an insertion-like sequence present in <i>Bradyrhizobium japonicum</i>, were generated by polymerase chain reaction (PCR) with two pairs of primers. The amount of each fragment generated by the multiplex PCR was proportional to the amount of template DNA present. The amplification of the 900-bp RSα fragment was more sensitive, since it was amplified from a smaller amount of template DNA than the 730-bp <i>nolBT</i> fragment. By running the multiplex reaction in the presence of template DNA isolated from different sources, we confirmed that the reaction can discriminate between <i>S. fredii</i>, <i>Bradyrhizobium japonicum</i> and <i>Sinorhizobium xinjiangensis</i>. |
| publishDate |
2003 |
| dc.date.none.fl_str_mv |
2003 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Articulo http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
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article |
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publishedVersion |
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http://sedici.unlp.edu.ar/handle/10915/84343 |
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http://sedici.unlp.edu.ar/handle/10915/84343 |
| dc.language.none.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/issn/0378-1097 info:eu-repo/semantics/altIdentifier/doi/10.1016/S0378-1097(03)00796-1 |
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openAccess |
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http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
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