Osteogenic actions of the anti-diabetic drug metformin on osteoblasts in culture
- Autores
- Cortizo, Ana María; Sedlinsky, Claudia; McCarthy, Antonio Desmond; Blanco, Alcira; Schurman, León
- Año de publicación
- 2006
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- An association has been previously established between uncompensated diabetes mellitus and the loss of bone mineral density and/or quality. In this study, we evaluated the effects of metformin on the growth and differentiation of osteoblasts in culture. Treatment of two osteoblast-like cells (UMR106 and MC3T3E1) with metformin (25–500μM) for 24h led to a dose-dependent increase of cell proliferation. Metformin also promoted osteoblastic differentiation: it increased type-I collagen production in both cell lines and stimulated alkaline phosphatase activity in MC3T3E1 osteoblasts. In addition, metformin markedly increased the formation of nodules of mineralization in 3-week MC3T3E1 cultures. Metformin induced activation and redistribution of phosphorylated extracellular signal-regulated kinase (P-ERK) in a transient manner, and dosedependently stimulated the expression of endothelial and inducible nitric oxide synthases (e/iNOS). These results show for the first time a direct osteogenic effect of metformin on osteoblasts in culture, which could be mediated by activation/redistribution of ERK-1/2 and induction of e/ iNOS.
Facultad de Ciencias Exactas - Materia
-
Ciencias Exactas
Metformin; Osteoblast; Osteoporosis; Diabetes mellitus
Metformina
Osteoblastos - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by/4.0/
- Repositorio
- Institución
- Universidad Nacional de La Plata
- OAI Identificador
- oai:sedici.unlp.edu.ar:10915/76260
Ver los metadatos del registro completo
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Osteogenic actions of the anti-diabetic drug metformin on osteoblasts in cultureCortizo, Ana MaríaSedlinsky, ClaudiaMcCarthy, Antonio DesmondBlanco, AlciraSchurman, LeónCiencias ExactasMetformin; Osteoblast; Osteoporosis; Diabetes mellitusMetforminaOsteoblastosAn association has been previously established between uncompensated diabetes mellitus and the loss of bone mineral density and/or quality. In this study, we evaluated the effects of metformin on the growth and differentiation of osteoblasts in culture. Treatment of two osteoblast-like cells (UMR106 and MC3T3E1) with metformin (25–500μM) for 24h led to a dose-dependent increase of cell proliferation. Metformin also promoted osteoblastic differentiation: it increased type-I collagen production in both cell lines and stimulated alkaline phosphatase activity in MC3T3E1 osteoblasts. In addition, metformin markedly increased the formation of nodules of mineralization in 3-week MC3T3E1 cultures. Metformin induced activation and redistribution of phosphorylated extracellular signal-regulated kinase (P-ERK) in a transient manner, and dosedependently stimulated the expression of endothelial and inducible nitric oxide synthases (e/iNOS). These results show for the first time a direct osteogenic effect of metformin on osteoblasts in culture, which could be mediated by activation/redistribution of ERK-1/2 and induction of e/ iNOS.Facultad de Ciencias Exactas2006info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf38-46http://sedici.unlp.edu.ar/handle/10915/76260enginfo:eu-repo/semantics/altIdentifier/issn/0014-2999info:eu-repo/semantics/altIdentifier/hdl/11746/4418info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ejphar.2006.02.030info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:13:25Zoai:sedici.unlp.edu.ar:10915/76260Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:13:25.794SEDICI (UNLP) - Universidad Nacional de La Platafalse |
dc.title.none.fl_str_mv |
Osteogenic actions of the anti-diabetic drug metformin on osteoblasts in culture |
title |
Osteogenic actions of the anti-diabetic drug metformin on osteoblasts in culture |
spellingShingle |
Osteogenic actions of the anti-diabetic drug metformin on osteoblasts in culture Cortizo, Ana María Ciencias Exactas Metformin; Osteoblast; Osteoporosis; Diabetes mellitus Metformina Osteoblastos |
title_short |
Osteogenic actions of the anti-diabetic drug metformin on osteoblasts in culture |
title_full |
Osteogenic actions of the anti-diabetic drug metformin on osteoblasts in culture |
title_fullStr |
Osteogenic actions of the anti-diabetic drug metformin on osteoblasts in culture |
title_full_unstemmed |
Osteogenic actions of the anti-diabetic drug metformin on osteoblasts in culture |
title_sort |
Osteogenic actions of the anti-diabetic drug metformin on osteoblasts in culture |
dc.creator.none.fl_str_mv |
Cortizo, Ana María Sedlinsky, Claudia McCarthy, Antonio Desmond Blanco, Alcira Schurman, León |
author |
Cortizo, Ana María |
author_facet |
Cortizo, Ana María Sedlinsky, Claudia McCarthy, Antonio Desmond Blanco, Alcira Schurman, León |
author_role |
author |
author2 |
Sedlinsky, Claudia McCarthy, Antonio Desmond Blanco, Alcira Schurman, León |
author2_role |
author author author author |
dc.subject.none.fl_str_mv |
Ciencias Exactas Metformin; Osteoblast; Osteoporosis; Diabetes mellitus Metformina Osteoblastos |
topic |
Ciencias Exactas Metformin; Osteoblast; Osteoporosis; Diabetes mellitus Metformina Osteoblastos |
dc.description.none.fl_txt_mv |
An association has been previously established between uncompensated diabetes mellitus and the loss of bone mineral density and/or quality. In this study, we evaluated the effects of metformin on the growth and differentiation of osteoblasts in culture. Treatment of two osteoblast-like cells (UMR106 and MC3T3E1) with metformin (25–500μM) for 24h led to a dose-dependent increase of cell proliferation. Metformin also promoted osteoblastic differentiation: it increased type-I collagen production in both cell lines and stimulated alkaline phosphatase activity in MC3T3E1 osteoblasts. In addition, metformin markedly increased the formation of nodules of mineralization in 3-week MC3T3E1 cultures. Metformin induced activation and redistribution of phosphorylated extracellular signal-regulated kinase (P-ERK) in a transient manner, and dosedependently stimulated the expression of endothelial and inducible nitric oxide synthases (e/iNOS). These results show for the first time a direct osteogenic effect of metformin on osteoblasts in culture, which could be mediated by activation/redistribution of ERK-1/2 and induction of e/ iNOS. Facultad de Ciencias Exactas |
description |
An association has been previously established between uncompensated diabetes mellitus and the loss of bone mineral density and/or quality. In this study, we evaluated the effects of metformin on the growth and differentiation of osteoblasts in culture. Treatment of two osteoblast-like cells (UMR106 and MC3T3E1) with metformin (25–500μM) for 24h led to a dose-dependent increase of cell proliferation. Metformin also promoted osteoblastic differentiation: it increased type-I collagen production in both cell lines and stimulated alkaline phosphatase activity in MC3T3E1 osteoblasts. In addition, metformin markedly increased the formation of nodules of mineralization in 3-week MC3T3E1 cultures. Metformin induced activation and redistribution of phosphorylated extracellular signal-regulated kinase (P-ERK) in a transient manner, and dosedependently stimulated the expression of endothelial and inducible nitric oxide synthases (e/iNOS). These results show for the first time a direct osteogenic effect of metformin on osteoblasts in culture, which could be mediated by activation/redistribution of ERK-1/2 and induction of e/ iNOS. |
publishDate |
2006 |
dc.date.none.fl_str_mv |
2006 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Articulo http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://sedici.unlp.edu.ar/handle/10915/76260 |
url |
http://sedici.unlp.edu.ar/handle/10915/76260 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/issn/0014-2999 info:eu-repo/semantics/altIdentifier/hdl/11746/4418 info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ejphar.2006.02.030 |
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info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/4.0/ Creative Commons Attribution 4.0 International (CC BY 4.0) |
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openAccess |
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http://creativecommons.org/licenses/by/4.0/ Creative Commons Attribution 4.0 International (CC BY 4.0) |
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application/pdf 38-46 |
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SEDICI (UNLP) - Universidad Nacional de La Plata |
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