Osteogenic actions of the anti-diabetic drug metformin on osteoblasts in culture

Autores
Cortizo, Ana María; Sedlinsky, Claudia; McCarthy, Antonio Desmond; Blanco, Alcira; Schurman, León
Año de publicación
2006
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
An association has been previously established between uncompensated diabetes mellitus and the loss of bone mineral density and/or quality. In this study, we evaluated the effects of metformin on the growth and differentiation of osteoblasts in culture. Treatment of two osteoblast-like cells (UMR106 and MC3T3E1) with metformin (25–500μM) for 24h led to a dose-dependent increase of cell proliferation. Metformin also promoted osteoblastic differentiation: it increased type-I collagen production in both cell lines and stimulated alkaline phosphatase activity in MC3T3E1 osteoblasts. In addition, metformin markedly increased the formation of nodules of mineralization in 3-week MC3T3E1 cultures. Metformin induced activation and redistribution of phosphorylated extracellular signal-regulated kinase (P-ERK) in a transient manner, and dosedependently stimulated the expression of endothelial and inducible nitric oxide synthases (e/iNOS). These results show for the first time a direct osteogenic effect of metformin on osteoblasts in culture, which could be mediated by activation/redistribution of ERK-1/2 and induction of e/ iNOS.
Facultad de Ciencias Exactas
Materia
Ciencias Exactas
Metformin; Osteoblast; Osteoporosis; Diabetes mellitus
Metformina
Osteoblastos
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/76260
Acceder
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network_name_str SEDICI (UNLP)
spelling Osteogenic actions of the anti-diabetic drug metformin on osteoblasts in cultureCortizo, Ana MaríaSedlinsky, ClaudiaMcCarthy, Antonio DesmondBlanco, AlciraSchurman, LeónCiencias ExactasMetformin; Osteoblast; Osteoporosis; Diabetes mellitusMetforminaOsteoblastosAn association has been previously established between uncompensated diabetes mellitus and the loss of bone mineral density and/or quality. In this study, we evaluated the effects of metformin on the growth and differentiation of osteoblasts in culture. Treatment of two osteoblast-like cells (UMR106 and MC3T3E1) with metformin (25–500μM) for 24h led to a dose-dependent increase of cell proliferation. Metformin also promoted osteoblastic differentiation: it increased type-I collagen production in both cell lines and stimulated alkaline phosphatase activity in MC3T3E1 osteoblasts. In addition, metformin markedly increased the formation of nodules of mineralization in 3-week MC3T3E1 cultures. Metformin induced activation and redistribution of phosphorylated extracellular signal-regulated kinase (P-ERK) in a transient manner, and dosedependently stimulated the expression of endothelial and inducible nitric oxide synthases (e/iNOS). These results show for the first time a direct osteogenic effect of metformin on osteoblasts in culture, which could be mediated by activation/redistribution of ERK-1/2 and induction of e/ iNOS.Facultad de Ciencias Exactas2006info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf38-46http://sedici.unlp.edu.ar/handle/10915/76260enginfo:eu-repo/semantics/altIdentifier/issn/0014-2999info:eu-repo/semantics/altIdentifier/hdl/11746/4418info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ejphar.2006.02.030info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-11-05T12:52:48Zoai:sedici.unlp.edu.ar:10915/76260Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-11-05 12:52:48.538SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Osteogenic actions of the anti-diabetic drug metformin on osteoblasts in culture
title Osteogenic actions of the anti-diabetic drug metformin on osteoblasts in culture
spellingShingle Osteogenic actions of the anti-diabetic drug metformin on osteoblasts in culture
Cortizo, Ana María
Ciencias Exactas
Metformin; Osteoblast; Osteoporosis; Diabetes mellitus
Metformina
Osteoblastos
title_short Osteogenic actions of the anti-diabetic drug metformin on osteoblasts in culture
title_full Osteogenic actions of the anti-diabetic drug metformin on osteoblasts in culture
title_fullStr Osteogenic actions of the anti-diabetic drug metformin on osteoblasts in culture
title_full_unstemmed Osteogenic actions of the anti-diabetic drug metformin on osteoblasts in culture
title_sort Osteogenic actions of the anti-diabetic drug metformin on osteoblasts in culture
dc.creator.none.fl_str_mv Cortizo, Ana María
Sedlinsky, Claudia
McCarthy, Antonio Desmond
Blanco, Alcira
Schurman, León
author Cortizo, Ana María
author_facet Cortizo, Ana María
Sedlinsky, Claudia
McCarthy, Antonio Desmond
Blanco, Alcira
Schurman, León
author_role author
author2 Sedlinsky, Claudia
McCarthy, Antonio Desmond
Blanco, Alcira
Schurman, León
author2_role author
author
author
author
dc.subject.none.fl_str_mv Ciencias Exactas
Metformin; Osteoblast; Osteoporosis; Diabetes mellitus
Metformina
Osteoblastos
topic Ciencias Exactas
Metformin; Osteoblast; Osteoporosis; Diabetes mellitus
Metformina
Osteoblastos
dc.description.none.fl_txt_mv An association has been previously established between uncompensated diabetes mellitus and the loss of bone mineral density and/or quality. In this study, we evaluated the effects of metformin on the growth and differentiation of osteoblasts in culture. Treatment of two osteoblast-like cells (UMR106 and MC3T3E1) with metformin (25–500μM) for 24h led to a dose-dependent increase of cell proliferation. Metformin also promoted osteoblastic differentiation: it increased type-I collagen production in both cell lines and stimulated alkaline phosphatase activity in MC3T3E1 osteoblasts. In addition, metformin markedly increased the formation of nodules of mineralization in 3-week MC3T3E1 cultures. Metformin induced activation and redistribution of phosphorylated extracellular signal-regulated kinase (P-ERK) in a transient manner, and dosedependently stimulated the expression of endothelial and inducible nitric oxide synthases (e/iNOS). These results show for the first time a direct osteogenic effect of metformin on osteoblasts in culture, which could be mediated by activation/redistribution of ERK-1/2 and induction of e/ iNOS.
Facultad de Ciencias Exactas
description An association has been previously established between uncompensated diabetes mellitus and the loss of bone mineral density and/or quality. In this study, we evaluated the effects of metformin on the growth and differentiation of osteoblasts in culture. Treatment of two osteoblast-like cells (UMR106 and MC3T3E1) with metformin (25–500μM) for 24h led to a dose-dependent increase of cell proliferation. Metformin also promoted osteoblastic differentiation: it increased type-I collagen production in both cell lines and stimulated alkaline phosphatase activity in MC3T3E1 osteoblasts. In addition, metformin markedly increased the formation of nodules of mineralization in 3-week MC3T3E1 cultures. Metformin induced activation and redistribution of phosphorylated extracellular signal-regulated kinase (P-ERK) in a transient manner, and dosedependently stimulated the expression of endothelial and inducible nitric oxide synthases (e/iNOS). These results show for the first time a direct osteogenic effect of metformin on osteoblasts in culture, which could be mediated by activation/redistribution of ERK-1/2 and induction of e/ iNOS.
publishDate 2006
dc.date.none.fl_str_mv 2006
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/76260
url http://sedici.unlp.edu.ar/handle/10915/76260
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/0014-2999
info:eu-repo/semantics/altIdentifier/hdl/11746/4418
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ejphar.2006.02.030
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
dc.format.none.fl_str_mv application/pdf
38-46
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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