Osteogenic actions of the anti-diabetic drug metformin on osteoblasts in culture

Autores
Cortizo, Ana María; Sedlinsky, Claudia; McCarthy, Antonio Desmond; Blanco, Alcira; Schurman, León
Año de publicación
2006
Idioma
inglés
Tipo de recurso
artículo
Estado
versión enviada
Descripción
An association has been previously established between uncompensated diabetes mellitus and the loss of bone mineral density and/or quality. In this study, we evaluated the effects of metformin on the growth and differentiation of osteoblasts in culture. Treatment of two osteoblast-like cells (UMR106 and MC3T3E1) with metformin (25–500μM) for 24h led to a dose-dependent increase of cell proliferation. Metformin also promoted osteoblastic differentiation: it increased type-I collagen production in both cell lines and stimulated alkaline phosphatase activity in MC3T3E1 osteoblasts. In addition, metformin markedly increased the formation of nodules of mineralization in 3-week MC3T3E1 cultures. Metformin induced activation and redistribution of phosphorylated extracellular signal-regulated kinase (P-ERK) in a transient manner, and dosedependently stimulated the expression of endothelial and inducible nitric oxide synthases (e/iNOS). These results show for the first time a direct osteogenic effect of metformin on osteoblasts in culture, which could be mediated by activation/redistribution of ERK-1/2 and induction of e/ iNOS.
Materia
Ciencias Químicas
Metformin
Osteoblast
Osteoporosis
Diabetes Mellitus
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
CIC Digital (CICBA)
Institución
Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
OAI Identificador
oai:digital.cic.gba.gob.ar:11746/4418

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repository_id_str 9441
network_name_str CIC Digital (CICBA)
spelling Osteogenic actions of the anti-diabetic drug metformin on osteoblasts in cultureCortizo, Ana MaríaSedlinsky, ClaudiaMcCarthy, Antonio DesmondBlanco, AlciraSchurman, LeónCiencias QuímicasMetforminOsteoblastOsteoporosisDiabetes MellitusAn association has been previously established between uncompensated diabetes mellitus and the loss of bone mineral density and/or quality. In this study, we evaluated the effects of metformin on the growth and differentiation of osteoblasts in culture. Treatment of two osteoblast-like cells (UMR106 and MC3T3E1) with metformin (25–500μM) for 24h led to a dose-dependent increase of cell proliferation. Metformin also promoted osteoblastic differentiation: it increased type-I collagen production in both cell lines and stimulated alkaline phosphatase activity in MC3T3E1 osteoblasts. In addition, metformin markedly increased the formation of nodules of mineralization in 3-week MC3T3E1 cultures. Metformin induced activation and redistribution of phosphorylated extracellular signal-regulated kinase (P-ERK) in a transient manner, and dosedependently stimulated the expression of endothelial and inducible nitric oxide synthases (e/iNOS). These results show for the first time a direct osteogenic effect of metformin on osteoblasts in culture, which could be mediated by activation/redistribution of ERK-1/2 and induction of e/ iNOS.2006info:eu-repo/semantics/articleinfo:eu-repo/semantics/submittedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://digital.cic.gba.gob.ar/handle/11746/4418enginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/reponame:CIC Digital (CICBA)instname:Comisión de Investigaciones Científicas de la Provincia de Buenos Airesinstacron:CICBA2025-09-29T13:40:19Zoai:digital.cic.gba.gob.ar:11746/4418Institucionalhttp://digital.cic.gba.gob.arOrganismo científico-tecnológicoNo correspondehttp://digital.cic.gba.gob.ar/oai/snrdmarisa.degiusti@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:94412025-09-29 13:40:19.69CIC Digital (CICBA) - Comisión de Investigaciones Científicas de la Provincia de Buenos Airesfalse
dc.title.none.fl_str_mv Osteogenic actions of the anti-diabetic drug metformin on osteoblasts in culture
title Osteogenic actions of the anti-diabetic drug metformin on osteoblasts in culture
spellingShingle Osteogenic actions of the anti-diabetic drug metformin on osteoblasts in culture
Cortizo, Ana María
Ciencias Químicas
Metformin
Osteoblast
Osteoporosis
Diabetes Mellitus
title_short Osteogenic actions of the anti-diabetic drug metformin on osteoblasts in culture
title_full Osteogenic actions of the anti-diabetic drug metformin on osteoblasts in culture
title_fullStr Osteogenic actions of the anti-diabetic drug metformin on osteoblasts in culture
title_full_unstemmed Osteogenic actions of the anti-diabetic drug metformin on osteoblasts in culture
title_sort Osteogenic actions of the anti-diabetic drug metformin on osteoblasts in culture
dc.creator.none.fl_str_mv Cortizo, Ana María
Sedlinsky, Claudia
McCarthy, Antonio Desmond
Blanco, Alcira
Schurman, León
author Cortizo, Ana María
author_facet Cortizo, Ana María
Sedlinsky, Claudia
McCarthy, Antonio Desmond
Blanco, Alcira
Schurman, León
author_role author
author2 Sedlinsky, Claudia
McCarthy, Antonio Desmond
Blanco, Alcira
Schurman, León
author2_role author
author
author
author
dc.subject.none.fl_str_mv Ciencias Químicas
Metformin
Osteoblast
Osteoporosis
Diabetes Mellitus
topic Ciencias Químicas
Metformin
Osteoblast
Osteoporosis
Diabetes Mellitus
dc.description.none.fl_txt_mv An association has been previously established between uncompensated diabetes mellitus and the loss of bone mineral density and/or quality. In this study, we evaluated the effects of metformin on the growth and differentiation of osteoblasts in culture. Treatment of two osteoblast-like cells (UMR106 and MC3T3E1) with metformin (25–500μM) for 24h led to a dose-dependent increase of cell proliferation. Metformin also promoted osteoblastic differentiation: it increased type-I collagen production in both cell lines and stimulated alkaline phosphatase activity in MC3T3E1 osteoblasts. In addition, metformin markedly increased the formation of nodules of mineralization in 3-week MC3T3E1 cultures. Metformin induced activation and redistribution of phosphorylated extracellular signal-regulated kinase (P-ERK) in a transient manner, and dosedependently stimulated the expression of endothelial and inducible nitric oxide synthases (e/iNOS). These results show for the first time a direct osteogenic effect of metformin on osteoblasts in culture, which could be mediated by activation/redistribution of ERK-1/2 and induction of e/ iNOS.
description An association has been previously established between uncompensated diabetes mellitus and the loss of bone mineral density and/or quality. In this study, we evaluated the effects of metformin on the growth and differentiation of osteoblasts in culture. Treatment of two osteoblast-like cells (UMR106 and MC3T3E1) with metformin (25–500μM) for 24h led to a dose-dependent increase of cell proliferation. Metformin also promoted osteoblastic differentiation: it increased type-I collagen production in both cell lines and stimulated alkaline phosphatase activity in MC3T3E1 osteoblasts. In addition, metformin markedly increased the formation of nodules of mineralization in 3-week MC3T3E1 cultures. Metformin induced activation and redistribution of phosphorylated extracellular signal-regulated kinase (P-ERK) in a transient manner, and dosedependently stimulated the expression of endothelial and inducible nitric oxide synthases (e/iNOS). These results show for the first time a direct osteogenic effect of metformin on osteoblasts in culture, which could be mediated by activation/redistribution of ERK-1/2 and induction of e/ iNOS.
publishDate 2006
dc.date.none.fl_str_mv 2006
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/submittedVersion
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info:ar-repo/semantics/articulo
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dc.identifier.none.fl_str_mv https://digital.cic.gba.gob.ar/handle/11746/4418
url https://digital.cic.gba.gob.ar/handle/11746/4418
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
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eu_rights_str_mv openAccess
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instname_str Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
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