Biomarkers of prolonged exposure to microcystin-LR in mice

Autores
Sedan, Daniela Yazmine; Giannuzzi, Leda; Rosso, Lorena; Marra, Carlos Alberto; Andrinolo, Darío
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The effects of prolonged exposure to microcystins (MCs) on health are not yet sufficiently understood and this type of poisoning is often undiagnosed. Even though chronic exposure has been linked with liver cancer and alterations have been described in liver damage marker enzymes in exposed populations, there are not profile parameters that indicate prolonged exposure to microcystins. The aim of this work is to determine, based on an animal model of prolonged exposure to successive i.p. doses of 25 mg MC-LR/kg body weight, several plasma parameters which could be useful as exposure biomarkers. Hemoglobin (Hb) and methemoglobin (MetHb) levels were determined on blood samples. We also studied plasma levels of hydroperoxides (ROOHs), a-tocopherol, glutathione and lipid profile as well as superoxide dismutase (SOD) and catalase (CAT) erythrocyte activities. In addition, the determination of MC-LR levels in liver, kidney, plasma, urine and feces of treated mice was carried out. We found that alteration in MetHb, ROOHs, glutathione, a-tocopherol levels, SOD activity and plasma lipid profile, correlates with those expected if the alteration derived from hepatic damage. The alterated plasma paramenters together with MC-LR determination could be used as biomarkers, helpful tools in screening and epidemiological studies.
Centro de Investigación y Desarrollo en Criotecnología de Alimentos
Instituto de Investigaciones Bioquímicas de La Plata
Materia
Ciencias Exactas
Microcystins
Prolonged exposure
Biomarkers
Mice
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/120414
Acceder
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oai_identifier_str oai:sedici.unlp.edu.ar:10915/120414
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Biomarkers of prolonged exposure to microcystin-LR in miceSedan, Daniela YazmineGiannuzzi, LedaRosso, LorenaMarra, Carlos AlbertoAndrinolo, DaríoCiencias ExactasMicrocystinsProlonged exposureBiomarkersMiceThe effects of prolonged exposure to microcystins (MCs) on health are not yet sufficiently understood and this type of poisoning is often undiagnosed. Even though chronic exposure has been linked with liver cancer and alterations have been described in liver damage marker enzymes in exposed populations, there are not profile parameters that indicate prolonged exposure to microcystins. The aim of this work is to determine, based on an animal model of prolonged exposure to successive i.p. doses of 25 mg MC-LR/kg body weight, several plasma parameters which could be useful as exposure biomarkers. Hemoglobin (Hb) and methemoglobin (MetHb) levels were determined on blood samples. We also studied plasma levels of hydroperoxides (ROOHs), a-tocopherol, glutathione and lipid profile as well as superoxide dismutase (SOD) and catalase (CAT) erythrocyte activities. In addition, the determination of MC-LR levels in liver, kidney, plasma, urine and feces of treated mice was carried out. We found that alteration in MetHb, ROOHs, glutathione, a-tocopherol levels, SOD activity and plasma lipid profile, correlates with those expected if the alteration derived from hepatic damage. The alterated plasma paramenters together with MC-LR determination could be used as biomarkers, helpful tools in screening and epidemiological studies.Centro de Investigación y Desarrollo en Criotecnología de AlimentosInstituto de Investigaciones Bioquímicas de La Plata2013info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf9-17http://sedici.unlp.edu.ar/handle/10915/120414enginfo:eu-repo/semantics/altIdentifier/issn/0041-0101info:eu-repo/semantics/altIdentifier/doi/10.1016/j.toxicon.2013.03.002info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:28:30Zoai:sedici.unlp.edu.ar:10915/120414Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:28:30.527SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Biomarkers of prolonged exposure to microcystin-LR in mice
title Biomarkers of prolonged exposure to microcystin-LR in mice
spellingShingle Biomarkers of prolonged exposure to microcystin-LR in mice
Sedan, Daniela Yazmine
Ciencias Exactas
Microcystins
Prolonged exposure
Biomarkers
Mice
title_short Biomarkers of prolonged exposure to microcystin-LR in mice
title_full Biomarkers of prolonged exposure to microcystin-LR in mice
title_fullStr Biomarkers of prolonged exposure to microcystin-LR in mice
title_full_unstemmed Biomarkers of prolonged exposure to microcystin-LR in mice
title_sort Biomarkers of prolonged exposure to microcystin-LR in mice
dc.creator.none.fl_str_mv Sedan, Daniela Yazmine
Giannuzzi, Leda
Rosso, Lorena
Marra, Carlos Alberto
Andrinolo, Darío
author Sedan, Daniela Yazmine
author_facet Sedan, Daniela Yazmine
Giannuzzi, Leda
Rosso, Lorena
Marra, Carlos Alberto
Andrinolo, Darío
author_role author
author2 Giannuzzi, Leda
Rosso, Lorena
Marra, Carlos Alberto
Andrinolo, Darío
author2_role author
author
author
author
dc.subject.none.fl_str_mv Ciencias Exactas
Microcystins
Prolonged exposure
Biomarkers
Mice
topic Ciencias Exactas
Microcystins
Prolonged exposure
Biomarkers
Mice
dc.description.none.fl_txt_mv The effects of prolonged exposure to microcystins (MCs) on health are not yet sufficiently understood and this type of poisoning is often undiagnosed. Even though chronic exposure has been linked with liver cancer and alterations have been described in liver damage marker enzymes in exposed populations, there are not profile parameters that indicate prolonged exposure to microcystins. The aim of this work is to determine, based on an animal model of prolonged exposure to successive i.p. doses of 25 mg MC-LR/kg body weight, several plasma parameters which could be useful as exposure biomarkers. Hemoglobin (Hb) and methemoglobin (MetHb) levels were determined on blood samples. We also studied plasma levels of hydroperoxides (ROOHs), a-tocopherol, glutathione and lipid profile as well as superoxide dismutase (SOD) and catalase (CAT) erythrocyte activities. In addition, the determination of MC-LR levels in liver, kidney, plasma, urine and feces of treated mice was carried out. We found that alteration in MetHb, ROOHs, glutathione, a-tocopherol levels, SOD activity and plasma lipid profile, correlates with those expected if the alteration derived from hepatic damage. The alterated plasma paramenters together with MC-LR determination could be used as biomarkers, helpful tools in screening and epidemiological studies.
Centro de Investigación y Desarrollo en Criotecnología de Alimentos
Instituto de Investigaciones Bioquímicas de La Plata
description The effects of prolonged exposure to microcystins (MCs) on health are not yet sufficiently understood and this type of poisoning is often undiagnosed. Even though chronic exposure has been linked with liver cancer and alterations have been described in liver damage marker enzymes in exposed populations, there are not profile parameters that indicate prolonged exposure to microcystins. The aim of this work is to determine, based on an animal model of prolonged exposure to successive i.p. doses of 25 mg MC-LR/kg body weight, several plasma parameters which could be useful as exposure biomarkers. Hemoglobin (Hb) and methemoglobin (MetHb) levels were determined on blood samples. We also studied plasma levels of hydroperoxides (ROOHs), a-tocopherol, glutathione and lipid profile as well as superoxide dismutase (SOD) and catalase (CAT) erythrocyte activities. In addition, the determination of MC-LR levels in liver, kidney, plasma, urine and feces of treated mice was carried out. We found that alteration in MetHb, ROOHs, glutathione, a-tocopherol levels, SOD activity and plasma lipid profile, correlates with those expected if the alteration derived from hepatic damage. The alterated plasma paramenters together with MC-LR determination could be used as biomarkers, helpful tools in screening and epidemiological studies.
publishDate 2013
dc.date.none.fl_str_mv 2013
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/120414
url http://sedici.unlp.edu.ar/handle/10915/120414
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/0041-0101
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.toxicon.2013.03.002
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
dc.format.none.fl_str_mv application/pdf
9-17
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
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reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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