Plasmatic Alterations derived from liver damage caused by prolonged exposure to Microcystin-LR in mice
- Autores
- Sedan, Daniela Yazmine; Giannuzzi, Leda; Rosso, Lorena; Marra, Carlos Alberto; Andrinolo, Dario
- Año de publicación
- 2013
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The effects of prolonged exposure to microcystins (MCs) on health are not yet sufficiently understood and this type of poisoning is often undiagnosed. Even though chronic exposure has been linked with liver cancer and alterations have been described in liver damage marker enzymes in exposed populations, there are not profile parameters that indicate prolonged exposure to microcystins. The aim of this work is to determine, based on an animal model of prolonged exposure to successive i.p. doses of 25 mg MC-LR/kg body weight, several plasma parameters which could be useful as exposure biomarkers. Hemoglobin (Hb) and methemoglobin (MetHb) levels were determined on blood samples. We also studied plasma levels of hydroperoxides (ROOHs), a-tocopherol, glutathione and lipid profile as well as superoxide dismutase (SOD) and catalase (CAT) erythrocyte activities. In addition, the determination of MC-LR levels in liver, kidney, plasma, urine and feces of treated mice was carried out. We found that alteration in MetHb, ROOHs, glutathione, a-tocopherol levels, SOD activity and plasma lipid profile, correlates with those expected if the alteration derived from hepatic damage. The alterated plasma paramenters together with MC-LR determination could be used as biomarkers, helpful tools in screening and epidemiological studies.
Fil: Sedan, Daniela Yazmine. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Area de Toxicología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Giannuzzi, Leda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Centro de Investigaciones en Criotecnología de Alimentos (i); Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Area de Toxicología; Argentina
Fil: Rosso, Lorena. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Area de Toxicología; Argentina
Fil: Marra, Carlos Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Instituto de Investigaciones Bioquímicas de la Plata; Argentina
Fil: Andrinolo, Dario. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Area de Toxicología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina - Materia
-
BIOMARKERS
MICE
MICROCYSTINS
PROLONGED EXPOSURE - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/2361
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oai:ri.conicet.gov.ar:11336/2361 |
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CONICET Digital (CONICET) |
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Plasmatic Alterations derived from liver damage caused by prolonged exposure to Microcystin-LR in miceSedan, Daniela YazmineGiannuzzi, LedaRosso, LorenaMarra, Carlos AlbertoAndrinolo, DarioBIOMARKERSMICEMICROCYSTINSPROLONGED EXPOSUREhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1https://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3The effects of prolonged exposure to microcystins (MCs) on health are not yet sufficiently understood and this type of poisoning is often undiagnosed. Even though chronic exposure has been linked with liver cancer and alterations have been described in liver damage marker enzymes in exposed populations, there are not profile parameters that indicate prolonged exposure to microcystins. The aim of this work is to determine, based on an animal model of prolonged exposure to successive i.p. doses of 25 mg MC-LR/kg body weight, several plasma parameters which could be useful as exposure biomarkers. Hemoglobin (Hb) and methemoglobin (MetHb) levels were determined on blood samples. We also studied plasma levels of hydroperoxides (ROOHs), a-tocopherol, glutathione and lipid profile as well as superoxide dismutase (SOD) and catalase (CAT) erythrocyte activities. In addition, the determination of MC-LR levels in liver, kidney, plasma, urine and feces of treated mice was carried out. We found that alteration in MetHb, ROOHs, glutathione, a-tocopherol levels, SOD activity and plasma lipid profile, correlates with those expected if the alteration derived from hepatic damage. The alterated plasma paramenters together with MC-LR determination could be used as biomarkers, helpful tools in screening and epidemiological studies.Fil: Sedan, Daniela Yazmine. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Area de Toxicología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Giannuzzi, Leda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Centro de Investigaciones en Criotecnología de Alimentos (i); Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Area de Toxicología; ArgentinaFil: Rosso, Lorena. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Area de Toxicología; ArgentinaFil: Marra, Carlos Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Instituto de Investigaciones Bioquímicas de la Plata; ArgentinaFil: Andrinolo, Dario. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Area de Toxicología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaElsevier2013-03-15info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/2361Sedan, Daniela Yazmine; Giannuzzi, Leda; Rosso, Lorena; Marra, Carlos Alberto; Andrinolo, Dario; Plasmatic Alterations derived from liver damage caused by prolonged exposure to Microcystin-LR in mice; Elsevier; Toxicon; 68; 15-3-2013; 9-170041-0101enginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0041010113000883info:eu-repo/semantics/altIdentifier/doi/10.1016/j.toxicon.2013.03.002info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:27:26Zoai:ri.conicet.gov.ar:11336/2361instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:27:26.453CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Plasmatic Alterations derived from liver damage caused by prolonged exposure to Microcystin-LR in mice |
title |
Plasmatic Alterations derived from liver damage caused by prolonged exposure to Microcystin-LR in mice |
spellingShingle |
Plasmatic Alterations derived from liver damage caused by prolonged exposure to Microcystin-LR in mice Sedan, Daniela Yazmine BIOMARKERS MICE MICROCYSTINS PROLONGED EXPOSURE |
title_short |
Plasmatic Alterations derived from liver damage caused by prolonged exposure to Microcystin-LR in mice |
title_full |
Plasmatic Alterations derived from liver damage caused by prolonged exposure to Microcystin-LR in mice |
title_fullStr |
Plasmatic Alterations derived from liver damage caused by prolonged exposure to Microcystin-LR in mice |
title_full_unstemmed |
Plasmatic Alterations derived from liver damage caused by prolonged exposure to Microcystin-LR in mice |
title_sort |
Plasmatic Alterations derived from liver damage caused by prolonged exposure to Microcystin-LR in mice |
dc.creator.none.fl_str_mv |
Sedan, Daniela Yazmine Giannuzzi, Leda Rosso, Lorena Marra, Carlos Alberto Andrinolo, Dario |
author |
Sedan, Daniela Yazmine |
author_facet |
Sedan, Daniela Yazmine Giannuzzi, Leda Rosso, Lorena Marra, Carlos Alberto Andrinolo, Dario |
author_role |
author |
author2 |
Giannuzzi, Leda Rosso, Lorena Marra, Carlos Alberto Andrinolo, Dario |
author2_role |
author author author author |
dc.subject.none.fl_str_mv |
BIOMARKERS MICE MICROCYSTINS PROLONGED EXPOSURE |
topic |
BIOMARKERS MICE MICROCYSTINS PROLONGED EXPOSURE |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 https://purl.org/becyt/ford/3.1 https://purl.org/becyt/ford/3 |
dc.description.none.fl_txt_mv |
The effects of prolonged exposure to microcystins (MCs) on health are not yet sufficiently understood and this type of poisoning is often undiagnosed. Even though chronic exposure has been linked with liver cancer and alterations have been described in liver damage marker enzymes in exposed populations, there are not profile parameters that indicate prolonged exposure to microcystins. The aim of this work is to determine, based on an animal model of prolonged exposure to successive i.p. doses of 25 mg MC-LR/kg body weight, several plasma parameters which could be useful as exposure biomarkers. Hemoglobin (Hb) and methemoglobin (MetHb) levels were determined on blood samples. We also studied plasma levels of hydroperoxides (ROOHs), a-tocopherol, glutathione and lipid profile as well as superoxide dismutase (SOD) and catalase (CAT) erythrocyte activities. In addition, the determination of MC-LR levels in liver, kidney, plasma, urine and feces of treated mice was carried out. We found that alteration in MetHb, ROOHs, glutathione, a-tocopherol levels, SOD activity and plasma lipid profile, correlates with those expected if the alteration derived from hepatic damage. The alterated plasma paramenters together with MC-LR determination could be used as biomarkers, helpful tools in screening and epidemiological studies. Fil: Sedan, Daniela Yazmine. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Area de Toxicología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Giannuzzi, Leda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Centro de Investigaciones en Criotecnología de Alimentos (i); Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Area de Toxicología; Argentina Fil: Rosso, Lorena. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Area de Toxicología; Argentina Fil: Marra, Carlos Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Instituto de Investigaciones Bioquímicas de la Plata; Argentina Fil: Andrinolo, Dario. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Area de Toxicología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina |
description |
The effects of prolonged exposure to microcystins (MCs) on health are not yet sufficiently understood and this type of poisoning is often undiagnosed. Even though chronic exposure has been linked with liver cancer and alterations have been described in liver damage marker enzymes in exposed populations, there are not profile parameters that indicate prolonged exposure to microcystins. The aim of this work is to determine, based on an animal model of prolonged exposure to successive i.p. doses of 25 mg MC-LR/kg body weight, several plasma parameters which could be useful as exposure biomarkers. Hemoglobin (Hb) and methemoglobin (MetHb) levels were determined on blood samples. We also studied plasma levels of hydroperoxides (ROOHs), a-tocopherol, glutathione and lipid profile as well as superoxide dismutase (SOD) and catalase (CAT) erythrocyte activities. In addition, the determination of MC-LR levels in liver, kidney, plasma, urine and feces of treated mice was carried out. We found that alteration in MetHb, ROOHs, glutathione, a-tocopherol levels, SOD activity and plasma lipid profile, correlates with those expected if the alteration derived from hepatic damage. The alterated plasma paramenters together with MC-LR determination could be used as biomarkers, helpful tools in screening and epidemiological studies. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-03-15 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/2361 Sedan, Daniela Yazmine; Giannuzzi, Leda; Rosso, Lorena; Marra, Carlos Alberto; Andrinolo, Dario; Plasmatic Alterations derived from liver damage caused by prolonged exposure to Microcystin-LR in mice; Elsevier; Toxicon; 68; 15-3-2013; 9-17 0041-0101 |
url |
http://hdl.handle.net/11336/2361 |
identifier_str_mv |
Sedan, Daniela Yazmine; Giannuzzi, Leda; Rosso, Lorena; Marra, Carlos Alberto; Andrinolo, Dario; Plasmatic Alterations derived from liver damage caused by prolonged exposure to Microcystin-LR in mice; Elsevier; Toxicon; 68; 15-3-2013; 9-17 0041-0101 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0041010113000883 info:eu-repo/semantics/altIdentifier/doi/10.1016/j.toxicon.2013.03.002 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844614276189257728 |
score |
13.070432 |