Hepatic recovery after damage produced by sub-chronic intoxication with the cyanotoxin microcystin LR

Autores
Andrinolo, Darío; Sedan, Daniela Yazmine; Telese, Luis Horacio; Aura, Claudia; Masera, Silvia; Giannuzzi, Leda; Marra, Carlos Alberto; Tacconi de Alaniz, María Josefa
Año de publicación
2008
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The effect of sub-chronic exposure of intraperitoneal (i.p.) injections of microcystin-LR (MC-LR) on microscopic tissue architecture, hepatic function and lipid peroxidation has been studied in liver and kidney of mice. Mice were treated i.p. with 25 mg of pure MC-LR/kg body weight or saline solution for 1 month (every 2 days) with the aim of producing an inflictive stage with evident damage. Histopathological analysis of dissected livers of mice showed a disrupted lobar architecture and the development of cytoplasmatic vacuoles. According to this, a significant increase in hepatic lipid content and in lipid peroxidation levels in liver and kidney was found in MC-LR-treated animals when compared with controls. Moreover, serum alkaline phosphatase and aspartate aminotransferase activities showed a significant alteration in MC-LR-treated animals. After damage, progression or recovery was studied for 1 and 2 months of wash-out. The recovery from liver damage was evident at the cytological and physiological level, only the recovery of lobar architecture was incomplete along the period investigated. In conclusion, the present study demonstrates the ability of hepatic tissue to recover from damage produced by sub-chronic MC-LR administration. The dynamic interplay between damage and tissue-repairing response in determining the ultimate outcome of toxicity should be considered in risk-assessment studies.
Centro de Investigación y Desarrollo en Criotecnología de Alimentos
Facultad de Ciencias Exactas
Instituto de Investigaciones Bioquímicas de La Plata
Materia
Química
Microcystins
Sub-chronic exposure liver recovery
Lipid peroxidation
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/114951
Acceder
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oai_identifier_str oai:sedici.unlp.edu.ar:10915/114951
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Hepatic recovery after damage produced by sub-chronic intoxication with the cyanotoxin microcystin LRAndrinolo, DaríoSedan, Daniela YazmineTelese, Luis HoracioAura, ClaudiaMasera, SilviaGiannuzzi, LedaMarra, Carlos AlbertoTacconi de Alaniz, María JosefaQuímicaMicrocystinsSub-chronic exposure liver recoveryLipid peroxidationThe effect of sub-chronic exposure of intraperitoneal (i.p.) injections of microcystin-LR (MC-LR) on microscopic tissue architecture, hepatic function and lipid peroxidation has been studied in liver and kidney of mice. Mice were treated i.p. with 25 mg of pure MC-LR/kg body weight or saline solution for 1 month (every 2 days) with the aim of producing an inflictive stage with evident damage. Histopathological analysis of dissected livers of mice showed a disrupted lobar architecture and the development of cytoplasmatic vacuoles. According to this, a significant increase in hepatic lipid content and in lipid peroxidation levels in liver and kidney was found in MC-LR-treated animals when compared with controls. Moreover, serum alkaline phosphatase and aspartate aminotransferase activities showed a significant alteration in MC-LR-treated animals. After damage, progression or recovery was studied for 1 and 2 months of wash-out. The recovery from liver damage was evident at the cytological and physiological level, only the recovery of lobar architecture was incomplete along the period investigated. In conclusion, the present study demonstrates the ability of hepatic tissue to recover from damage produced by sub-chronic MC-LR administration. The dynamic interplay between damage and tissue-repairing response in determining the ultimate outcome of toxicity should be considered in risk-assessment studies.Centro de Investigación y Desarrollo en Criotecnología de AlimentosFacultad de Ciencias ExactasInstituto de Investigaciones Bioquímicas de La Plata2008info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf457-467http://sedici.unlp.edu.ar/handle/10915/114951enginfo:eu-repo/semantics/altIdentifier/issn/0041-0101info:eu-repo/semantics/altIdentifier/doi/10.1016/j.toxicon.2007.11.012info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:26:45Zoai:sedici.unlp.edu.ar:10915/114951Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:26:45.696SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Hepatic recovery after damage produced by sub-chronic intoxication with the cyanotoxin microcystin LR
title Hepatic recovery after damage produced by sub-chronic intoxication with the cyanotoxin microcystin LR
spellingShingle Hepatic recovery after damage produced by sub-chronic intoxication with the cyanotoxin microcystin LR
Andrinolo, Darío
Química
Microcystins
Sub-chronic exposure liver recovery
Lipid peroxidation
title_short Hepatic recovery after damage produced by sub-chronic intoxication with the cyanotoxin microcystin LR
title_full Hepatic recovery after damage produced by sub-chronic intoxication with the cyanotoxin microcystin LR
title_fullStr Hepatic recovery after damage produced by sub-chronic intoxication with the cyanotoxin microcystin LR
title_full_unstemmed Hepatic recovery after damage produced by sub-chronic intoxication with the cyanotoxin microcystin LR
title_sort Hepatic recovery after damage produced by sub-chronic intoxication with the cyanotoxin microcystin LR
dc.creator.none.fl_str_mv Andrinolo, Darío
Sedan, Daniela Yazmine
Telese, Luis Horacio
Aura, Claudia
Masera, Silvia
Giannuzzi, Leda
Marra, Carlos Alberto
Tacconi de Alaniz, María Josefa
author Andrinolo, Darío
author_facet Andrinolo, Darío
Sedan, Daniela Yazmine
Telese, Luis Horacio
Aura, Claudia
Masera, Silvia
Giannuzzi, Leda
Marra, Carlos Alberto
Tacconi de Alaniz, María Josefa
author_role author
author2 Sedan, Daniela Yazmine
Telese, Luis Horacio
Aura, Claudia
Masera, Silvia
Giannuzzi, Leda
Marra, Carlos Alberto
Tacconi de Alaniz, María Josefa
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Química
Microcystins
Sub-chronic exposure liver recovery
Lipid peroxidation
topic Química
Microcystins
Sub-chronic exposure liver recovery
Lipid peroxidation
dc.description.none.fl_txt_mv The effect of sub-chronic exposure of intraperitoneal (i.p.) injections of microcystin-LR (MC-LR) on microscopic tissue architecture, hepatic function and lipid peroxidation has been studied in liver and kidney of mice. Mice were treated i.p. with 25 mg of pure MC-LR/kg body weight or saline solution for 1 month (every 2 days) with the aim of producing an inflictive stage with evident damage. Histopathological analysis of dissected livers of mice showed a disrupted lobar architecture and the development of cytoplasmatic vacuoles. According to this, a significant increase in hepatic lipid content and in lipid peroxidation levels in liver and kidney was found in MC-LR-treated animals when compared with controls. Moreover, serum alkaline phosphatase and aspartate aminotransferase activities showed a significant alteration in MC-LR-treated animals. After damage, progression or recovery was studied for 1 and 2 months of wash-out. The recovery from liver damage was evident at the cytological and physiological level, only the recovery of lobar architecture was incomplete along the period investigated. In conclusion, the present study demonstrates the ability of hepatic tissue to recover from damage produced by sub-chronic MC-LR administration. The dynamic interplay between damage and tissue-repairing response in determining the ultimate outcome of toxicity should be considered in risk-assessment studies.
Centro de Investigación y Desarrollo en Criotecnología de Alimentos
Facultad de Ciencias Exactas
Instituto de Investigaciones Bioquímicas de La Plata
description The effect of sub-chronic exposure of intraperitoneal (i.p.) injections of microcystin-LR (MC-LR) on microscopic tissue architecture, hepatic function and lipid peroxidation has been studied in liver and kidney of mice. Mice were treated i.p. with 25 mg of pure MC-LR/kg body weight or saline solution for 1 month (every 2 days) with the aim of producing an inflictive stage with evident damage. Histopathological analysis of dissected livers of mice showed a disrupted lobar architecture and the development of cytoplasmatic vacuoles. According to this, a significant increase in hepatic lipid content and in lipid peroxidation levels in liver and kidney was found in MC-LR-treated animals when compared with controls. Moreover, serum alkaline phosphatase and aspartate aminotransferase activities showed a significant alteration in MC-LR-treated animals. After damage, progression or recovery was studied for 1 and 2 months of wash-out. The recovery from liver damage was evident at the cytological and physiological level, only the recovery of lobar architecture was incomplete along the period investigated. In conclusion, the present study demonstrates the ability of hepatic tissue to recover from damage produced by sub-chronic MC-LR administration. The dynamic interplay between damage and tissue-repairing response in determining the ultimate outcome of toxicity should be considered in risk-assessment studies.
publishDate 2008
dc.date.none.fl_str_mv 2008
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/114951
url http://sedici.unlp.edu.ar/handle/10915/114951
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/0041-0101
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.toxicon.2007.11.012
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
dc.format.none.fl_str_mv application/pdf
457-467
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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score 13.070432

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