A <i>gua</i>B mutant strain of <i>Rhizobium tropici</i> CIAT899 pleiotropically defective in thermal tolerance and symbiosis

Autores
Riccillo, Pablo Miguel; Collavino, Mónica Mariana; Grasso, Daniel Horacio; England, Reg; Bruijn, Frans J. de; Aguilar, Orlando Mario
Año de publicación
2000
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Rhizobium tropici strain CIAT899 displays a high intrinsic thermal tolerance, and had been used in this work to study the molecular basis of bacterial responses to high temperature. We generated a collection of R. tropici CIAT899 mutants affected in thermal tolerance using Tn5-luxAB mutagenesis and described the characterization of a mutant strain, CIAT899-10T, that fails to grow under conditions of high temperature. Strain CIAT899-10T carries a single transposon insertion in a gene showing a high degree of similarity with the guaB gene of Escherichia coli and other organisms, encoding the enzyme inosine monophosphate dehydrogenase. The guaB strain CIAT899-10T does not require guanine for growth due to an alternative pathway via xanthine dehydrogenase and, phenotypically, in addition to the thermal sensitivity, the mutant is also defective in symbiosis with beans, forming nodules that lack rhizobial content. Guanine and its precursors restore wild-type tolerance to grow at high temperature. Our data show that, in R. tropici, the production of guanine via inosine monophosphate dehydrogenase is essential for growth at extreme temperatures and for effective nodulation.
Facultad de Ciencias Exactas
Instituto de Biotecnologia y Biologia Molecular
Materia
Ciencias Exactas
Biología
guanine biosynthesis
nodule development
prototrophy
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/124099
Acceder
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oai_identifier_str oai:sedici.unlp.edu.ar:10915/124099
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling A <i>gua</i>B mutant strain of <i>Rhizobium tropici</i> CIAT899 pleiotropically defective in thermal tolerance and symbiosisRiccillo, Pablo MiguelCollavino, Mónica MarianaGrasso, Daniel HoracioEngland, RegBruijn, Frans J. deAguilar, Orlando MarioCiencias ExactasBiologíaguanine biosynthesisnodule developmentprototrophy<i>Rhizobium tropici</i> strain CIAT899 displays a high intrinsic thermal tolerance, and had been used in this work to study the molecular basis of bacterial responses to high temperature. We generated a collection of <i>R. tropici</i> CIAT899 mutants affected in thermal tolerance using Tn5-<i>lux</i>AB mutagenesis and described the characterization of a mutant strain, CIAT899-10T, that fails to grow under conditions of high temperature. Strain CIAT899-10T carries a single transposon insertion in a gene showing a high degree of similarity with the <i>gua</i>B gene of <i>Escherichia coli</i> and other organisms, encoding the enzyme inosine monophosphate dehydrogenase. The <i>gua</i>B strain CIAT899-10T does not require guanine for growth due to an alternative pathway via xanthine dehydrogenase and, phenotypically, in addition to the thermal sensitivity, the mutant is also defective in symbiosis with beans, forming nodules that lack rhizobial content. Guanine and its precursors restore wild-type tolerance to grow at high temperature. Our data show that, in <i>R. tropici</i>, the production of guanine via inosine monophosphate dehydrogenase is essential for growth at extreme temperatures and for effective nodulation.Facultad de Ciencias ExactasInstituto de Biotecnologia y Biologia Molecular2000-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf1228-1236http://sedici.unlp.edu.ar/handle/10915/124099enginfo:eu-repo/semantics/altIdentifier/issn/0894-0282info:eu-repo/semantics/altIdentifier/pmid/11059489info:eu-repo/semantics/altIdentifier/doi/10.1094/mpmi.2000.13.11.1228info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:29:33Zoai:sedici.unlp.edu.ar:10915/124099Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:29:33.681SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv A <i>gua</i>B mutant strain of <i>Rhizobium tropici</i> CIAT899 pleiotropically defective in thermal tolerance and symbiosis
title A <i>gua</i>B mutant strain of <i>Rhizobium tropici</i> CIAT899 pleiotropically defective in thermal tolerance and symbiosis
spellingShingle A <i>gua</i>B mutant strain of <i>Rhizobium tropici</i> CIAT899 pleiotropically defective in thermal tolerance and symbiosis
Riccillo, Pablo Miguel
Ciencias Exactas
Biología
guanine biosynthesis
nodule development
prototrophy
title_short A <i>gua</i>B mutant strain of <i>Rhizobium tropici</i> CIAT899 pleiotropically defective in thermal tolerance and symbiosis
title_full A <i>gua</i>B mutant strain of <i>Rhizobium tropici</i> CIAT899 pleiotropically defective in thermal tolerance and symbiosis
title_fullStr A <i>gua</i>B mutant strain of <i>Rhizobium tropici</i> CIAT899 pleiotropically defective in thermal tolerance and symbiosis
title_full_unstemmed A <i>gua</i>B mutant strain of <i>Rhizobium tropici</i> CIAT899 pleiotropically defective in thermal tolerance and symbiosis
title_sort A <i>gua</i>B mutant strain of <i>Rhizobium tropici</i> CIAT899 pleiotropically defective in thermal tolerance and symbiosis
dc.creator.none.fl_str_mv Riccillo, Pablo Miguel
Collavino, Mónica Mariana
Grasso, Daniel Horacio
England, Reg
Bruijn, Frans J. de
Aguilar, Orlando Mario
author Riccillo, Pablo Miguel
author_facet Riccillo, Pablo Miguel
Collavino, Mónica Mariana
Grasso, Daniel Horacio
England, Reg
Bruijn, Frans J. de
Aguilar, Orlando Mario
author_role author
author2 Collavino, Mónica Mariana
Grasso, Daniel Horacio
England, Reg
Bruijn, Frans J. de
Aguilar, Orlando Mario
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Ciencias Exactas
Biología
guanine biosynthesis
nodule development
prototrophy
topic Ciencias Exactas
Biología
guanine biosynthesis
nodule development
prototrophy
dc.description.none.fl_txt_mv <i>Rhizobium tropici</i> strain CIAT899 displays a high intrinsic thermal tolerance, and had been used in this work to study the molecular basis of bacterial responses to high temperature. We generated a collection of <i>R. tropici</i> CIAT899 mutants affected in thermal tolerance using Tn5-<i>lux</i>AB mutagenesis and described the characterization of a mutant strain, CIAT899-10T, that fails to grow under conditions of high temperature. Strain CIAT899-10T carries a single transposon insertion in a gene showing a high degree of similarity with the <i>gua</i>B gene of <i>Escherichia coli</i> and other organisms, encoding the enzyme inosine monophosphate dehydrogenase. The <i>gua</i>B strain CIAT899-10T does not require guanine for growth due to an alternative pathway via xanthine dehydrogenase and, phenotypically, in addition to the thermal sensitivity, the mutant is also defective in symbiosis with beans, forming nodules that lack rhizobial content. Guanine and its precursors restore wild-type tolerance to grow at high temperature. Our data show that, in <i>R. tropici</i>, the production of guanine via inosine monophosphate dehydrogenase is essential for growth at extreme temperatures and for effective nodulation.
Facultad de Ciencias Exactas
Instituto de Biotecnologia y Biologia Molecular
description <i>Rhizobium tropici</i> strain CIAT899 displays a high intrinsic thermal tolerance, and had been used in this work to study the molecular basis of bacterial responses to high temperature. We generated a collection of <i>R. tropici</i> CIAT899 mutants affected in thermal tolerance using Tn5-<i>lux</i>AB mutagenesis and described the characterization of a mutant strain, CIAT899-10T, that fails to grow under conditions of high temperature. Strain CIAT899-10T carries a single transposon insertion in a gene showing a high degree of similarity with the <i>gua</i>B gene of <i>Escherichia coli</i> and other organisms, encoding the enzyme inosine monophosphate dehydrogenase. The <i>gua</i>B strain CIAT899-10T does not require guanine for growth due to an alternative pathway via xanthine dehydrogenase and, phenotypically, in addition to the thermal sensitivity, the mutant is also defective in symbiosis with beans, forming nodules that lack rhizobial content. Guanine and its precursors restore wild-type tolerance to grow at high temperature. Our data show that, in <i>R. tropici</i>, the production of guanine via inosine monophosphate dehydrogenase is essential for growth at extreme temperatures and for effective nodulation.
publishDate 2000
dc.date.none.fl_str_mv 2000-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/124099
url http://sedici.unlp.edu.ar/handle/10915/124099
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/0894-0282
info:eu-repo/semantics/altIdentifier/pmid/11059489
info:eu-repo/semantics/altIdentifier/doi/10.1094/mpmi.2000.13.11.1228
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
1228-1236
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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