High NaCl Concentrations Induce the nod Genes of Rhizobium tropici CIAT899 in the Absence of Flavonoid Inducers
- Autores
- Guasch Vidal, B.; Estévez, J.; Dardanelli, Marta Susana; Soria Díaz, M. E.; Fernández de Córdoba, F.; Balog, C. I. A.; Manyani, H.; Gil Serrano, A.; Thomas Oates, J.; Hensbergen, P. J.; Deelder, A. M.; Megías, M.; Brussel, A. A. N. van
- Año de publicación
- 2013
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The nodulation (nod) genes of Rhizobium tropici CIAT899 can be induced by very low concentrations (micromolar to nanomolar range) of several flavonoid molecules secreted by the roots of leguminous plants under a number of different conditions. Some of these conditions have been investigated and appear to have a great influence on the concentration and the number of different Nod factors, which can induce root nodule primordia and pseudonodules in several leguminous plant roots. In one such condition, we added up to 300 mM NaCl to the induction medium of R. tropici CIAT899 containing the nod gene inducer apigenin. At the higher concentrations of NaCl, larger amounts and more different Nod factors were produced than in the absence of extra NaCl. To our surprise, under control conditions (300 mM NaCl without apigenin), some Nod-factor-like spots were also observed on the thin-layer plates used to detect incorporation of radiolabeled glucosamine into newly synthesized Nod factors. This phenomenon was further investigated with thin-layer plates, fusions of nod genes to the lacZ gene, high-performance liquid chromatography, mass spectrometry, and the formation of pseudonodules on bean roots. Here, we report that, in the absence of flavonoid inducers, high concentrations of NaCl induced nod genes and the production of Nod factors.
Fil: Guasch Vidal, B.. Universidad de Sevilla; España
Fil: Estévez, J.. Universidad de Sevilla; España
Fil: Dardanelli, Marta Susana. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Soria Díaz, M. E.. Universidad de Sevilla; España
Fil: Fernández de Córdoba, F.. Universidad de Sevilla; España
Fil: Balog, C. I. A.. Leiden University; Países Bajos
Fil: Manyani, H.. Universidad de Sevilla; España
Fil: Gil Serrano, A.. Universidad de Sevilla; España
Fil: Thomas Oates, J.. University of York; Reino Unido
Fil: Hensbergen, P. J.. Leiden University; Países Bajos
Fil: Deelder, A. M.. Leiden University; Países Bajos
Fil: Megías, M.. Universidad de Sevilla; España
Fil: Brussel, A. A. N. van. Leiden University; Países Bajos - Materia
-
Phaseolus vulgaris
Rhizobium
Stress
Nod factor - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/24137
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3498 |
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CONICET Digital (CONICET) |
spelling |
High NaCl Concentrations Induce the nod Genes of Rhizobium tropici CIAT899 in the Absence of Flavonoid InducersGuasch Vidal, B.Estévez, J.Dardanelli, Marta SusanaSoria Díaz, M. E.Fernández de Córdoba, F.Balog, C. I. A.Manyani, H.Gil Serrano, A.Thomas Oates, J.Hensbergen, P. J.Deelder, A. M.Megías, M.Brussel, A. A. N. vanPhaseolus vulgarisRhizobiumStressNod factorThe nodulation (nod) genes of Rhizobium tropici CIAT899 can be induced by very low concentrations (micromolar to nanomolar range) of several flavonoid molecules secreted by the roots of leguminous plants under a number of different conditions. Some of these conditions have been investigated and appear to have a great influence on the concentration and the number of different Nod factors, which can induce root nodule primordia and pseudonodules in several leguminous plant roots. In one such condition, we added up to 300 mM NaCl to the induction medium of R. tropici CIAT899 containing the nod gene inducer apigenin. At the higher concentrations of NaCl, larger amounts and more different Nod factors were produced than in the absence of extra NaCl. To our surprise, under control conditions (300 mM NaCl without apigenin), some Nod-factor-like spots were also observed on the thin-layer plates used to detect incorporation of radiolabeled glucosamine into newly synthesized Nod factors. This phenomenon was further investigated with thin-layer plates, fusions of nod genes to the lacZ gene, high-performance liquid chromatography, mass spectrometry, and the formation of pseudonodules on bean roots. Here, we report that, in the absence of flavonoid inducers, high concentrations of NaCl induced nod genes and the production of Nod factors.Fil: Guasch Vidal, B.. Universidad de Sevilla; EspañaFil: Estévez, J.. Universidad de Sevilla; EspañaFil: Dardanelli, Marta Susana. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Soria Díaz, M. E.. Universidad de Sevilla; EspañaFil: Fernández de Córdoba, F.. Universidad de Sevilla; EspañaFil: Balog, C. I. A.. Leiden University; Países BajosFil: Manyani, H.. Universidad de Sevilla; EspañaFil: Gil Serrano, A.. Universidad de Sevilla; EspañaFil: Thomas Oates, J.. University of York; Reino UnidoFil: Hensbergen, P. J.. Leiden University; Países BajosFil: Deelder, A. M.. Leiden University; Países BajosFil: Megías, M.. Universidad de Sevilla; EspañaFil: Brussel, A. A. N. van. Leiden University; Países BajosAmerican Phytopathological Society2013-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/24137Guasch Vidal, B.; Estévez, J.; Dardanelli, Marta Susana; Soria Díaz, M. E.; Fernández de Córdoba, F.; et al.; High NaCl Concentrations Induce the nod Genes of Rhizobium tropici CIAT899 in the Absence of Flavonoid Inducers; American Phytopathological Society; Molecular Plant-Microbe Interactions; 26; 4; 4-2013; 451-4600894-0282CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1094/MPMI-09-12-0213-Rinfo:eu-repo/semantics/altIdentifier/url/http://apsjournals.apsnet.org/doi/10.1094/MPMI-09-12-0213-Rinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:01:00Zoai:ri.conicet.gov.ar:11336/24137instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:01:01.116CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
High NaCl Concentrations Induce the nod Genes of Rhizobium tropici CIAT899 in the Absence of Flavonoid Inducers |
title |
High NaCl Concentrations Induce the nod Genes of Rhizobium tropici CIAT899 in the Absence of Flavonoid Inducers |
spellingShingle |
High NaCl Concentrations Induce the nod Genes of Rhizobium tropici CIAT899 in the Absence of Flavonoid Inducers Guasch Vidal, B. Phaseolus vulgaris Rhizobium Stress Nod factor |
title_short |
High NaCl Concentrations Induce the nod Genes of Rhizobium tropici CIAT899 in the Absence of Flavonoid Inducers |
title_full |
High NaCl Concentrations Induce the nod Genes of Rhizobium tropici CIAT899 in the Absence of Flavonoid Inducers |
title_fullStr |
High NaCl Concentrations Induce the nod Genes of Rhizobium tropici CIAT899 in the Absence of Flavonoid Inducers |
title_full_unstemmed |
High NaCl Concentrations Induce the nod Genes of Rhizobium tropici CIAT899 in the Absence of Flavonoid Inducers |
title_sort |
High NaCl Concentrations Induce the nod Genes of Rhizobium tropici CIAT899 in the Absence of Flavonoid Inducers |
dc.creator.none.fl_str_mv |
Guasch Vidal, B. Estévez, J. Dardanelli, Marta Susana Soria Díaz, M. E. Fernández de Córdoba, F. Balog, C. I. A. Manyani, H. Gil Serrano, A. Thomas Oates, J. Hensbergen, P. J. Deelder, A. M. Megías, M. Brussel, A. A. N. van |
author |
Guasch Vidal, B. |
author_facet |
Guasch Vidal, B. Estévez, J. Dardanelli, Marta Susana Soria Díaz, M. E. Fernández de Córdoba, F. Balog, C. I. A. Manyani, H. Gil Serrano, A. Thomas Oates, J. Hensbergen, P. J. Deelder, A. M. Megías, M. Brussel, A. A. N. van |
author_role |
author |
author2 |
Estévez, J. Dardanelli, Marta Susana Soria Díaz, M. E. Fernández de Córdoba, F. Balog, C. I. A. Manyani, H. Gil Serrano, A. Thomas Oates, J. Hensbergen, P. J. Deelder, A. M. Megías, M. Brussel, A. A. N. van |
author2_role |
author author author author author author author author author author author author |
dc.subject.none.fl_str_mv |
Phaseolus vulgaris Rhizobium Stress Nod factor |
topic |
Phaseolus vulgaris Rhizobium Stress Nod factor |
dc.description.none.fl_txt_mv |
The nodulation (nod) genes of Rhizobium tropici CIAT899 can be induced by very low concentrations (micromolar to nanomolar range) of several flavonoid molecules secreted by the roots of leguminous plants under a number of different conditions. Some of these conditions have been investigated and appear to have a great influence on the concentration and the number of different Nod factors, which can induce root nodule primordia and pseudonodules in several leguminous plant roots. In one such condition, we added up to 300 mM NaCl to the induction medium of R. tropici CIAT899 containing the nod gene inducer apigenin. At the higher concentrations of NaCl, larger amounts and more different Nod factors were produced than in the absence of extra NaCl. To our surprise, under control conditions (300 mM NaCl without apigenin), some Nod-factor-like spots were also observed on the thin-layer plates used to detect incorporation of radiolabeled glucosamine into newly synthesized Nod factors. This phenomenon was further investigated with thin-layer plates, fusions of nod genes to the lacZ gene, high-performance liquid chromatography, mass spectrometry, and the formation of pseudonodules on bean roots. Here, we report that, in the absence of flavonoid inducers, high concentrations of NaCl induced nod genes and the production of Nod factors. Fil: Guasch Vidal, B.. Universidad de Sevilla; España Fil: Estévez, J.. Universidad de Sevilla; España Fil: Dardanelli, Marta Susana. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Soria Díaz, M. E.. Universidad de Sevilla; España Fil: Fernández de Córdoba, F.. Universidad de Sevilla; España Fil: Balog, C. I. A.. Leiden University; Países Bajos Fil: Manyani, H.. Universidad de Sevilla; España Fil: Gil Serrano, A.. Universidad de Sevilla; España Fil: Thomas Oates, J.. University of York; Reino Unido Fil: Hensbergen, P. J.. Leiden University; Países Bajos Fil: Deelder, A. M.. Leiden University; Países Bajos Fil: Megías, M.. Universidad de Sevilla; España Fil: Brussel, A. A. N. van. Leiden University; Países Bajos |
description |
The nodulation (nod) genes of Rhizobium tropici CIAT899 can be induced by very low concentrations (micromolar to nanomolar range) of several flavonoid molecules secreted by the roots of leguminous plants under a number of different conditions. Some of these conditions have been investigated and appear to have a great influence on the concentration and the number of different Nod factors, which can induce root nodule primordia and pseudonodules in several leguminous plant roots. In one such condition, we added up to 300 mM NaCl to the induction medium of R. tropici CIAT899 containing the nod gene inducer apigenin. At the higher concentrations of NaCl, larger amounts and more different Nod factors were produced than in the absence of extra NaCl. To our surprise, under control conditions (300 mM NaCl without apigenin), some Nod-factor-like spots were also observed on the thin-layer plates used to detect incorporation of radiolabeled glucosamine into newly synthesized Nod factors. This phenomenon was further investigated with thin-layer plates, fusions of nod genes to the lacZ gene, high-performance liquid chromatography, mass spectrometry, and the formation of pseudonodules on bean roots. Here, we report that, in the absence of flavonoid inducers, high concentrations of NaCl induced nod genes and the production of Nod factors. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-04 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/24137 Guasch Vidal, B.; Estévez, J.; Dardanelli, Marta Susana; Soria Díaz, M. E.; Fernández de Córdoba, F.; et al.; High NaCl Concentrations Induce the nod Genes of Rhizobium tropici CIAT899 in the Absence of Flavonoid Inducers; American Phytopathological Society; Molecular Plant-Microbe Interactions; 26; 4; 4-2013; 451-460 0894-0282 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/24137 |
identifier_str_mv |
Guasch Vidal, B.; Estévez, J.; Dardanelli, Marta Susana; Soria Díaz, M. E.; Fernández de Córdoba, F.; et al.; High NaCl Concentrations Induce the nod Genes of Rhizobium tropici CIAT899 in the Absence of Flavonoid Inducers; American Phytopathological Society; Molecular Plant-Microbe Interactions; 26; 4; 4-2013; 451-460 0894-0282 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.1094/MPMI-09-12-0213-R info:eu-repo/semantics/altIdentifier/url/http://apsjournals.apsnet.org/doi/10.1094/MPMI-09-12-0213-R |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
American Phytopathological Society |
publisher.none.fl_str_mv |
American Phytopathological Society |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844613798294454272 |
score |
13.070432 |