High NaCl Concentrations Induce the nod Genes of Rhizobium tropici CIAT899 in the Absence of Flavonoid Inducers

Autores
Guasch Vidal, B.; Estévez, J.; Dardanelli, Marta Susana; Soria Díaz, M. E.; Fernández de Córdoba, F.; Balog, C. I. A.; Manyani, H.; Gil Serrano, A.; Thomas Oates, J.; Hensbergen, P. J.; Deelder, A. M.; Megías, M.; Brussel, A. A. N. van
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The nodulation (nod) genes of Rhizobium tropici CIAT899 can be induced by very low concentrations (micromolar to nanomolar range) of several flavonoid molecules secreted by the roots of leguminous plants under a number of different conditions. Some of these conditions have been investigated and appear to have a great influence on the concentration and the number of different Nod factors, which can induce root nodule primordia and pseudonodules in several leguminous plant roots. In one such condition, we added up to 300 mM NaCl to the induction medium of R. tropici CIAT899 containing the nod gene inducer apigenin. At the higher concentrations of NaCl, larger amounts and more different Nod factors were produced than in the absence of extra NaCl. To our surprise, under control conditions (300 mM NaCl without apigenin), some Nod-factor-like spots were also observed on the thin-layer plates used to detect incorporation of radiolabeled glucosamine into newly synthesized Nod factors. This phenomenon was further investigated with thin-layer plates, fusions of nod genes to the lacZ gene, high-performance liquid chromatography, mass spectrometry, and the formation of pseudonodules on bean roots. Here, we report that, in the absence of flavonoid inducers, high concentrations of NaCl induced nod genes and the production of Nod factors.
Fil: Guasch Vidal, B.. Universidad de Sevilla; España
Fil: Estévez, J.. Universidad de Sevilla; España
Fil: Dardanelli, Marta Susana. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Soria Díaz, M. E.. Universidad de Sevilla; España
Fil: Fernández de Córdoba, F.. Universidad de Sevilla; España
Fil: Balog, C. I. A.. Leiden University; Países Bajos
Fil: Manyani, H.. Universidad de Sevilla; España
Fil: Gil Serrano, A.. Universidad de Sevilla; España
Fil: Thomas Oates, J.. University of York; Reino Unido
Fil: Hensbergen, P. J.. Leiden University; Países Bajos
Fil: Deelder, A. M.. Leiden University; Países Bajos
Fil: Megías, M.. Universidad de Sevilla; España
Fil: Brussel, A. A. N. van. Leiden University; Países Bajos
Materia
Phaseolus vulgaris
Rhizobium
Stress
Nod factor
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/24137
Acceder
id CONICETDig_e34785497c8a3ff6ec12ea37d4482e5e
oai_identifier_str oai:ri.conicet.gov.ar:11336/24137
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling High NaCl Concentrations Induce the nod Genes of Rhizobium tropici CIAT899 in the Absence of Flavonoid InducersGuasch Vidal, B.Estévez, J.Dardanelli, Marta SusanaSoria Díaz, M. E.Fernández de Córdoba, F.Balog, C. I. A.Manyani, H.Gil Serrano, A.Thomas Oates, J.Hensbergen, P. J.Deelder, A. M.Megías, M.Brussel, A. A. N. vanPhaseolus vulgarisRhizobiumStressNod factorThe nodulation (nod) genes of Rhizobium tropici CIAT899 can be induced by very low concentrations (micromolar to nanomolar range) of several flavonoid molecules secreted by the roots of leguminous plants under a number of different conditions. Some of these conditions have been investigated and appear to have a great influence on the concentration and the number of different Nod factors, which can induce root nodule primordia and pseudonodules in several leguminous plant roots. In one such condition, we added up to 300 mM NaCl to the induction medium of R. tropici CIAT899 containing the nod gene inducer apigenin. At the higher concentrations of NaCl, larger amounts and more different Nod factors were produced than in the absence of extra NaCl. To our surprise, under control conditions (300 mM NaCl without apigenin), some Nod-factor-like spots were also observed on the thin-layer plates used to detect incorporation of radiolabeled glucosamine into newly synthesized Nod factors. This phenomenon was further investigated with thin-layer plates, fusions of nod genes to the lacZ gene, high-performance liquid chromatography, mass spectrometry, and the formation of pseudonodules on bean roots. Here, we report that, in the absence of flavonoid inducers, high concentrations of NaCl induced nod genes and the production of Nod factors.Fil: Guasch Vidal, B.. Universidad de Sevilla; EspañaFil: Estévez, J.. Universidad de Sevilla; EspañaFil: Dardanelli, Marta Susana. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Soria Díaz, M. E.. Universidad de Sevilla; EspañaFil: Fernández de Córdoba, F.. Universidad de Sevilla; EspañaFil: Balog, C. I. A.. Leiden University; Países BajosFil: Manyani, H.. Universidad de Sevilla; EspañaFil: Gil Serrano, A.. Universidad de Sevilla; EspañaFil: Thomas Oates, J.. University of York; Reino UnidoFil: Hensbergen, P. J.. Leiden University; Países BajosFil: Deelder, A. M.. Leiden University; Países BajosFil: Megías, M.. Universidad de Sevilla; EspañaFil: Brussel, A. A. N. van. Leiden University; Países BajosAmerican Phytopathological Society2013-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/24137Guasch Vidal, B.; Estévez, J.; Dardanelli, Marta Susana; Soria Díaz, M. E.; Fernández de Córdoba, F.; et al.; High NaCl Concentrations Induce the nod Genes of Rhizobium tropici CIAT899 in the Absence of Flavonoid Inducers; American Phytopathological Society; Molecular Plant-Microbe Interactions; 26; 4; 4-2013; 451-4600894-0282CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1094/MPMI-09-12-0213-Rinfo:eu-repo/semantics/altIdentifier/url/http://apsjournals.apsnet.org/doi/10.1094/MPMI-09-12-0213-Rinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:56:59Zoai:ri.conicet.gov.ar:11336/24137instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:57:00.134CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv High NaCl Concentrations Induce the nod Genes of Rhizobium tropici CIAT899 in the Absence of Flavonoid Inducers
title High NaCl Concentrations Induce the nod Genes of Rhizobium tropici CIAT899 in the Absence of Flavonoid Inducers
spellingShingle High NaCl Concentrations Induce the nod Genes of Rhizobium tropici CIAT899 in the Absence of Flavonoid Inducers
Guasch Vidal, B.
Phaseolus vulgaris
Rhizobium
Stress
Nod factor
title_short High NaCl Concentrations Induce the nod Genes of Rhizobium tropici CIAT899 in the Absence of Flavonoid Inducers
title_full High NaCl Concentrations Induce the nod Genes of Rhizobium tropici CIAT899 in the Absence of Flavonoid Inducers
title_fullStr High NaCl Concentrations Induce the nod Genes of Rhizobium tropici CIAT899 in the Absence of Flavonoid Inducers
title_full_unstemmed High NaCl Concentrations Induce the nod Genes of Rhizobium tropici CIAT899 in the Absence of Flavonoid Inducers
title_sort High NaCl Concentrations Induce the nod Genes of Rhizobium tropici CIAT899 in the Absence of Flavonoid Inducers
dc.creator.none.fl_str_mv Guasch Vidal, B.
Estévez, J.
Dardanelli, Marta Susana
Soria Díaz, M. E.
Fernández de Córdoba, F.
Balog, C. I. A.
Manyani, H.
Gil Serrano, A.
Thomas Oates, J.
Hensbergen, P. J.
Deelder, A. M.
Megías, M.
Brussel, A. A. N. van
author Guasch Vidal, B.
author_facet Guasch Vidal, B.
Estévez, J.
Dardanelli, Marta Susana
Soria Díaz, M. E.
Fernández de Córdoba, F.
Balog, C. I. A.
Manyani, H.
Gil Serrano, A.
Thomas Oates, J.
Hensbergen, P. J.
Deelder, A. M.
Megías, M.
Brussel, A. A. N. van
author_role author
author2 Estévez, J.
Dardanelli, Marta Susana
Soria Díaz, M. E.
Fernández de Córdoba, F.
Balog, C. I. A.
Manyani, H.
Gil Serrano, A.
Thomas Oates, J.
Hensbergen, P. J.
Deelder, A. M.
Megías, M.
Brussel, A. A. N. van
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Phaseolus vulgaris
Rhizobium
Stress
Nod factor
topic Phaseolus vulgaris
Rhizobium
Stress
Nod factor
dc.description.none.fl_txt_mv The nodulation (nod) genes of Rhizobium tropici CIAT899 can be induced by very low concentrations (micromolar to nanomolar range) of several flavonoid molecules secreted by the roots of leguminous plants under a number of different conditions. Some of these conditions have been investigated and appear to have a great influence on the concentration and the number of different Nod factors, which can induce root nodule primordia and pseudonodules in several leguminous plant roots. In one such condition, we added up to 300 mM NaCl to the induction medium of R. tropici CIAT899 containing the nod gene inducer apigenin. At the higher concentrations of NaCl, larger amounts and more different Nod factors were produced than in the absence of extra NaCl. To our surprise, under control conditions (300 mM NaCl without apigenin), some Nod-factor-like spots were also observed on the thin-layer plates used to detect incorporation of radiolabeled glucosamine into newly synthesized Nod factors. This phenomenon was further investigated with thin-layer plates, fusions of nod genes to the lacZ gene, high-performance liquid chromatography, mass spectrometry, and the formation of pseudonodules on bean roots. Here, we report that, in the absence of flavonoid inducers, high concentrations of NaCl induced nod genes and the production of Nod factors.
Fil: Guasch Vidal, B.. Universidad de Sevilla; España
Fil: Estévez, J.. Universidad de Sevilla; España
Fil: Dardanelli, Marta Susana. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Soria Díaz, M. E.. Universidad de Sevilla; España
Fil: Fernández de Córdoba, F.. Universidad de Sevilla; España
Fil: Balog, C. I. A.. Leiden University; Países Bajos
Fil: Manyani, H.. Universidad de Sevilla; España
Fil: Gil Serrano, A.. Universidad de Sevilla; España
Fil: Thomas Oates, J.. University of York; Reino Unido
Fil: Hensbergen, P. J.. Leiden University; Países Bajos
Fil: Deelder, A. M.. Leiden University; Países Bajos
Fil: Megías, M.. Universidad de Sevilla; España
Fil: Brussel, A. A. N. van. Leiden University; Países Bajos
description The nodulation (nod) genes of Rhizobium tropici CIAT899 can be induced by very low concentrations (micromolar to nanomolar range) of several flavonoid molecules secreted by the roots of leguminous plants under a number of different conditions. Some of these conditions have been investigated and appear to have a great influence on the concentration and the number of different Nod factors, which can induce root nodule primordia and pseudonodules in several leguminous plant roots. In one such condition, we added up to 300 mM NaCl to the induction medium of R. tropici CIAT899 containing the nod gene inducer apigenin. At the higher concentrations of NaCl, larger amounts and more different Nod factors were produced than in the absence of extra NaCl. To our surprise, under control conditions (300 mM NaCl without apigenin), some Nod-factor-like spots were also observed on the thin-layer plates used to detect incorporation of radiolabeled glucosamine into newly synthesized Nod factors. This phenomenon was further investigated with thin-layer plates, fusions of nod genes to the lacZ gene, high-performance liquid chromatography, mass spectrometry, and the formation of pseudonodules on bean roots. Here, we report that, in the absence of flavonoid inducers, high concentrations of NaCl induced nod genes and the production of Nod factors.
publishDate 2013
dc.date.none.fl_str_mv 2013-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/24137
Guasch Vidal, B.; Estévez, J.; Dardanelli, Marta Susana; Soria Díaz, M. E.; Fernández de Córdoba, F.; et al.; High NaCl Concentrations Induce the nod Genes of Rhizobium tropici CIAT899 in the Absence of Flavonoid Inducers; American Phytopathological Society; Molecular Plant-Microbe Interactions; 26; 4; 4-2013; 451-460
0894-0282
CONICET Digital
CONICET
url http://hdl.handle.net/11336/24137
identifier_str_mv Guasch Vidal, B.; Estévez, J.; Dardanelli, Marta Susana; Soria Díaz, M. E.; Fernández de Córdoba, F.; et al.; High NaCl Concentrations Induce the nod Genes of Rhizobium tropici CIAT899 in the Absence of Flavonoid Inducers; American Phytopathological Society; Molecular Plant-Microbe Interactions; 26; 4; 4-2013; 451-460
0894-0282
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1094/MPMI-09-12-0213-R
info:eu-repo/semantics/altIdentifier/url/http://apsjournals.apsnet.org/doi/10.1094/MPMI-09-12-0213-R
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Phytopathological Society
publisher.none.fl_str_mv American Phytopathological Society
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846083106365243392
score 13.22299

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