Use of agro-industrial wastes for the production of a wild yeast enzyme with disintegration activity on plant tissues

Autores
Maidana, Silvana Andrea; Esteche, Vanesa Paola; Hours, Roque Alberto; Brumovsky, Luis Alberto; Martos, María Alicia
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The objective of the present study was to develop a cost-effective medium, using agro-industrial wastes for the production of a polygalacturonase by Wickerhanomyces anomalus of interest in cassava starch industries. The effect of several raw agro-industrial wastes and others nutrients on polygalacturonase production by W. anomalus, were evaluated, in a reference fermentation medium, using statistical designs, by batch culture. The ability of the cell-free supernatant to extract cassava starch was evaluated. Lemon peel was the best inducer for the production of PGase. Statistical analysis of the data showed that lemon peel, Mg⁺² and PO₄HK₂ had significant effect on PGase production, and the others variables (yeast extract, Ca⁺², Fe⁺², amino acid and trace element solution) were no significant. PGase synthesis reached ~31 EUmL⁻¹ , in the OFM (glucose, lemon peel, urea, vitamins, KH₂PO₄ and MgSO₄), after 12 h of culture, at a lab scale bioreactor. PGase of W. anomalus, was able to disintegrate cassava tuber tissue, and the starch granules contained within the cells were released into the reaction medium. Lemon peel can be used as inducer for PGase production by W. anomalus, in a low cost culture medium, appropriate for the production of the enzyme at large scale.
Centro de Investigación y Desarrollo en Fermentaciones Industriales
Materia
Química
Wickerhanomyces anómalus
Residues
Polygalacturonase
Cassava starch extraction
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/123711
Acceder
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oai_identifier_str oai:sedici.unlp.edu.ar:10915/123711
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Use of agro-industrial wastes for the production of a wild yeast enzyme with disintegration activity on plant tissuesMaidana, Silvana AndreaEsteche, Vanesa PaolaHours, Roque AlbertoBrumovsky, Luis AlbertoMartos, María AliciaQuímicaWickerhanomyces anómalusResiduesPolygalacturonaseCassava starch extractionThe objective of the present study was to develop a cost-effective medium, using agro-industrial wastes for the production of a polygalacturonase by Wickerhanomyces anomalus of interest in cassava starch industries. The effect of several raw agro-industrial wastes and others nutrients on polygalacturonase production by W. anomalus, were evaluated, in a reference fermentation medium, using statistical designs, by batch culture. The ability of the cell-free supernatant to extract cassava starch was evaluated. Lemon peel was the best inducer for the production of PGase. Statistical analysis of the data showed that lemon peel, Mg⁺² and PO₄HK₂ had significant effect on PGase production, and the others variables (yeast extract, Ca⁺², Fe⁺², amino acid and trace element solution) were no significant. PGase synthesis reached ~31 EUmL⁻¹ , in the OFM (glucose, lemon peel, urea, vitamins, KH₂PO₄ and MgSO₄), after 12 h of culture, at a lab scale bioreactor. PGase of W. anomalus, was able to disintegrate cassava tuber tissue, and the starch granules contained within the cells were released into the reaction medium. Lemon peel can be used as inducer for PGase production by W. anomalus, in a low cost culture medium, appropriate for the production of the enzyme at large scale.Centro de Investigación y Desarrollo en Fermentaciones Industriales2020info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://sedici.unlp.edu.ar/handle/10915/123711enginfo:eu-repo/semantics/altIdentifier/issn/1516-8913info:eu-repo/semantics/altIdentifier/issn/1678-4324info:eu-repo/semantics/altIdentifier/doi/10.1590/1678-4324-2020190515info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc/4.0/Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-03T11:01:29Zoai:sedici.unlp.edu.ar:10915/123711Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-03 11:01:30.265SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Use of agro-industrial wastes for the production of a wild yeast enzyme with disintegration activity on plant tissues
title Use of agro-industrial wastes for the production of a wild yeast enzyme with disintegration activity on plant tissues
spellingShingle Use of agro-industrial wastes for the production of a wild yeast enzyme with disintegration activity on plant tissues
Maidana, Silvana Andrea
Química
Wickerhanomyces anómalus
Residues
Polygalacturonase
Cassava starch extraction
title_short Use of agro-industrial wastes for the production of a wild yeast enzyme with disintegration activity on plant tissues
title_full Use of agro-industrial wastes for the production of a wild yeast enzyme with disintegration activity on plant tissues
title_fullStr Use of agro-industrial wastes for the production of a wild yeast enzyme with disintegration activity on plant tissues
title_full_unstemmed Use of agro-industrial wastes for the production of a wild yeast enzyme with disintegration activity on plant tissues
title_sort Use of agro-industrial wastes for the production of a wild yeast enzyme with disintegration activity on plant tissues
dc.creator.none.fl_str_mv Maidana, Silvana Andrea
Esteche, Vanesa Paola
Hours, Roque Alberto
Brumovsky, Luis Alberto
Martos, María Alicia
author Maidana, Silvana Andrea
author_facet Maidana, Silvana Andrea
Esteche, Vanesa Paola
Hours, Roque Alberto
Brumovsky, Luis Alberto
Martos, María Alicia
author_role author
author2 Esteche, Vanesa Paola
Hours, Roque Alberto
Brumovsky, Luis Alberto
Martos, María Alicia
author2_role author
author
author
author
dc.subject.none.fl_str_mv Química
Wickerhanomyces anómalus
Residues
Polygalacturonase
Cassava starch extraction
topic Química
Wickerhanomyces anómalus
Residues
Polygalacturonase
Cassava starch extraction
dc.description.none.fl_txt_mv The objective of the present study was to develop a cost-effective medium, using agro-industrial wastes for the production of a polygalacturonase by Wickerhanomyces anomalus of interest in cassava starch industries. The effect of several raw agro-industrial wastes and others nutrients on polygalacturonase production by W. anomalus, were evaluated, in a reference fermentation medium, using statistical designs, by batch culture. The ability of the cell-free supernatant to extract cassava starch was evaluated. Lemon peel was the best inducer for the production of PGase. Statistical analysis of the data showed that lemon peel, Mg⁺² and PO₄HK₂ had significant effect on PGase production, and the others variables (yeast extract, Ca⁺², Fe⁺², amino acid and trace element solution) were no significant. PGase synthesis reached ~31 EUmL⁻¹ , in the OFM (glucose, lemon peel, urea, vitamins, KH₂PO₄ and MgSO₄), after 12 h of culture, at a lab scale bioreactor. PGase of W. anomalus, was able to disintegrate cassava tuber tissue, and the starch granules contained within the cells were released into the reaction medium. Lemon peel can be used as inducer for PGase production by W. anomalus, in a low cost culture medium, appropriate for the production of the enzyme at large scale.
Centro de Investigación y Desarrollo en Fermentaciones Industriales
description The objective of the present study was to develop a cost-effective medium, using agro-industrial wastes for the production of a polygalacturonase by Wickerhanomyces anomalus of interest in cassava starch industries. The effect of several raw agro-industrial wastes and others nutrients on polygalacturonase production by W. anomalus, were evaluated, in a reference fermentation medium, using statistical designs, by batch culture. The ability of the cell-free supernatant to extract cassava starch was evaluated. Lemon peel was the best inducer for the production of PGase. Statistical analysis of the data showed that lemon peel, Mg⁺² and PO₄HK₂ had significant effect on PGase production, and the others variables (yeast extract, Ca⁺², Fe⁺², amino acid and trace element solution) were no significant. PGase synthesis reached ~31 EUmL⁻¹ , in the OFM (glucose, lemon peel, urea, vitamins, KH₂PO₄ and MgSO₄), after 12 h of culture, at a lab scale bioreactor. PGase of W. anomalus, was able to disintegrate cassava tuber tissue, and the starch granules contained within the cells were released into the reaction medium. Lemon peel can be used as inducer for PGase production by W. anomalus, in a low cost culture medium, appropriate for the production of the enzyme at large scale.
publishDate 2020
dc.date.none.fl_str_mv 2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/123711
url http://sedici.unlp.edu.ar/handle/10915/123711
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/1516-8913
info:eu-repo/semantics/altIdentifier/issn/1678-4324
info:eu-repo/semantics/altIdentifier/doi/10.1590/1678-4324-2020190515
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc/4.0/
Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc/4.0/
Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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score 13.13397

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