Use of agro-industrial wastes for the production of a wild yeast enzyme with disintegration activity on plant tissues
- Autores
- Maidana, Silvana Andrea; Esteche, Vanesa Paola; Hours, Roque Alberto; Brumovsky, Luis Alberto; Martos, María Alicia
- Año de publicación
- 2020
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The objective of the present study was to develop a cost-effective medium, using agro-industrial wastes for the production of a polygalacturonase by Wickerhanomyces anomalus of interest in cassava starch industries. The effect of several raw agro-industrial wastes and others nutrients on polygalacturonase production by W. anomalus, were evaluated, in a reference fermentation medium, using statistical designs, by batch culture. The ability of the cell-free supernatant to extract cassava starch was evaluated. Lemon peel was the best inducer for the production of PGase. Statistical analysis of the data showed that lemon peel, Mg+2 and PO4HK2 had significant effect on PGase production, and the others variables (yeast extract, Ca+2, Fe+2, amino acid and trace element solution) were no significant. PGase synthesis reached ~31 EUmL-1, in the OFM (glucose, lemon peel, urea, vitamins, KH2PO4 and MgSO4), after 12 h of culture, at a lab scale bioreactor. PGase of W. anomalus, was able to disintegrate cassava tuber tissue, and the starch granules contained within the cells were released into the reaction medium. Lemon peel can be used as inducer for PGase production by W. anomalus, in a low cost culture medium, appropriate for the production of the enzyme at large scale.
Fil: Maidana, Silvana Andrea. Universidad Nacional de Misiones. Facultad de Cs.exactas Químicas y Naturales. Departamento de Ciencia y Tecnol. de Los Alimentos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina
Fil: Esteche, Vanesa Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional de Misiones. Facultad de Cs.exactas Químicas y Naturales. Departamento de Ciencia y Tecnol. de Los Alimentos; Argentina
Fil: Hours, Roque Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina
Fil: Brumovsky, Luis Alberto. Universidad Nacional de Misiones. Facultad de Cs.exactas Químicas y Naturales. Departamento de Ciencia y Tecnol. de Los Alimentos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina
Fil: Martos, María Alicia. Universidad Nacional de Misiones. Facultad de Cs.exactas Químicas y Naturales. Departamento de Ciencia y Tecnol. de Los Alimentos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina - Materia
-
WICKERHANOMYCES ANÓMALUS
RESIDUES
POLYGALACTURONASE
CASSAVA STARCH EXTRACTION - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc/2.5/ar/
- Repositorio
.jpg)
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/142914
Ver los metadatos del registro completo
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Use of agro-industrial wastes for the production of a wild yeast enzyme with disintegration activity on plant tissuesMaidana, Silvana AndreaEsteche, Vanesa PaolaHours, Roque AlbertoBrumovsky, Luis AlbertoMartos, María AliciaWICKERHANOMYCES ANÓMALUSRESIDUESPOLYGALACTURONASECASSAVA STARCH EXTRACTIONhttps://purl.org/becyt/ford/2.9https://purl.org/becyt/ford/2The objective of the present study was to develop a cost-effective medium, using agro-industrial wastes for the production of a polygalacturonase by Wickerhanomyces anomalus of interest in cassava starch industries. The effect of several raw agro-industrial wastes and others nutrients on polygalacturonase production by W. anomalus, were evaluated, in a reference fermentation medium, using statistical designs, by batch culture. The ability of the cell-free supernatant to extract cassava starch was evaluated. Lemon peel was the best inducer for the production of PGase. Statistical analysis of the data showed that lemon peel, Mg+2 and PO4HK2 had significant effect on PGase production, and the others variables (yeast extract, Ca+2, Fe+2, amino acid and trace element solution) were no significant. PGase synthesis reached ~31 EUmL-1, in the OFM (glucose, lemon peel, urea, vitamins, KH2PO4 and MgSO4), after 12 h of culture, at a lab scale bioreactor. PGase of W. anomalus, was able to disintegrate cassava tuber tissue, and the starch granules contained within the cells were released into the reaction medium. Lemon peel can be used as inducer for PGase production by W. anomalus, in a low cost culture medium, appropriate for the production of the enzyme at large scale.Fil: Maidana, Silvana Andrea. Universidad Nacional de Misiones. Facultad de Cs.exactas Químicas y Naturales. Departamento de Ciencia y Tecnol. de Los Alimentos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; ArgentinaFil: Esteche, Vanesa Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional de Misiones. Facultad de Cs.exactas Químicas y Naturales. Departamento de Ciencia y Tecnol. de Los Alimentos; ArgentinaFil: Hours, Roque Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; ArgentinaFil: Brumovsky, Luis Alberto. Universidad Nacional de Misiones. Facultad de Cs.exactas Químicas y Naturales. Departamento de Ciencia y Tecnol. de Los Alimentos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; ArgentinaFil: Martos, María Alicia. Universidad Nacional de Misiones. Facultad de Cs.exactas Químicas y Naturales. Departamento de Ciencia y Tecnol. de Los Alimentos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; ArgentinaInstituto de Tecnologia do Paraná2020-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/142914Maidana, Silvana Andrea; Esteche, Vanesa Paola; Hours, Roque Alberto; Brumovsky, Luis Alberto; Martos, María Alicia; Use of agro-industrial wastes for the production of a wild yeast enzyme with disintegration activity on plant tissues; Instituto de Tecnologia do Paraná; Brazilian Archives of Biology and Technology; 63; 6-2020; 1-131516-89131678-4324CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.scielo.br/j/babt/a/SRfPks575Vskdx9wBTG5jhy/?lang=eninfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:52:40Zoai:ri.conicet.gov.ar:11336/142914instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:52:41.098CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
| dc.title.none.fl_str_mv |
Use of agro-industrial wastes for the production of a wild yeast enzyme with disintegration activity on plant tissues |
| title |
Use of agro-industrial wastes for the production of a wild yeast enzyme with disintegration activity on plant tissues |
| spellingShingle |
Use of agro-industrial wastes for the production of a wild yeast enzyme with disintegration activity on plant tissues Maidana, Silvana Andrea WICKERHANOMYCES ANÓMALUS RESIDUES POLYGALACTURONASE CASSAVA STARCH EXTRACTION |
| title_short |
Use of agro-industrial wastes for the production of a wild yeast enzyme with disintegration activity on plant tissues |
| title_full |
Use of agro-industrial wastes for the production of a wild yeast enzyme with disintegration activity on plant tissues |
| title_fullStr |
Use of agro-industrial wastes for the production of a wild yeast enzyme with disintegration activity on plant tissues |
| title_full_unstemmed |
Use of agro-industrial wastes for the production of a wild yeast enzyme with disintegration activity on plant tissues |
| title_sort |
Use of agro-industrial wastes for the production of a wild yeast enzyme with disintegration activity on plant tissues |
| dc.creator.none.fl_str_mv |
Maidana, Silvana Andrea Esteche, Vanesa Paola Hours, Roque Alberto Brumovsky, Luis Alberto Martos, María Alicia |
| author |
Maidana, Silvana Andrea |
| author_facet |
Maidana, Silvana Andrea Esteche, Vanesa Paola Hours, Roque Alberto Brumovsky, Luis Alberto Martos, María Alicia |
| author_role |
author |
| author2 |
Esteche, Vanesa Paola Hours, Roque Alberto Brumovsky, Luis Alberto Martos, María Alicia |
| author2_role |
author author author author |
| dc.subject.none.fl_str_mv |
WICKERHANOMYCES ANÓMALUS RESIDUES POLYGALACTURONASE CASSAVA STARCH EXTRACTION |
| topic |
WICKERHANOMYCES ANÓMALUS RESIDUES POLYGALACTURONASE CASSAVA STARCH EXTRACTION |
| purl_subject.fl_str_mv |
https://purl.org/becyt/ford/2.9 https://purl.org/becyt/ford/2 |
| dc.description.none.fl_txt_mv |
The objective of the present study was to develop a cost-effective medium, using agro-industrial wastes for the production of a polygalacturonase by Wickerhanomyces anomalus of interest in cassava starch industries. The effect of several raw agro-industrial wastes and others nutrients on polygalacturonase production by W. anomalus, were evaluated, in a reference fermentation medium, using statistical designs, by batch culture. The ability of the cell-free supernatant to extract cassava starch was evaluated. Lemon peel was the best inducer for the production of PGase. Statistical analysis of the data showed that lemon peel, Mg+2 and PO4HK2 had significant effect on PGase production, and the others variables (yeast extract, Ca+2, Fe+2, amino acid and trace element solution) were no significant. PGase synthesis reached ~31 EUmL-1, in the OFM (glucose, lemon peel, urea, vitamins, KH2PO4 and MgSO4), after 12 h of culture, at a lab scale bioreactor. PGase of W. anomalus, was able to disintegrate cassava tuber tissue, and the starch granules contained within the cells were released into the reaction medium. Lemon peel can be used as inducer for PGase production by W. anomalus, in a low cost culture medium, appropriate for the production of the enzyme at large scale. Fil: Maidana, Silvana Andrea. Universidad Nacional de Misiones. Facultad de Cs.exactas Químicas y Naturales. Departamento de Ciencia y Tecnol. de Los Alimentos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina Fil: Esteche, Vanesa Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional de Misiones. Facultad de Cs.exactas Químicas y Naturales. Departamento de Ciencia y Tecnol. de Los Alimentos; Argentina Fil: Hours, Roque Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina Fil: Brumovsky, Luis Alberto. Universidad Nacional de Misiones. Facultad de Cs.exactas Químicas y Naturales. Departamento de Ciencia y Tecnol. de Los Alimentos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina Fil: Martos, María Alicia. Universidad Nacional de Misiones. Facultad de Cs.exactas Químicas y Naturales. Departamento de Ciencia y Tecnol. de Los Alimentos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina |
| description |
The objective of the present study was to develop a cost-effective medium, using agro-industrial wastes for the production of a polygalacturonase by Wickerhanomyces anomalus of interest in cassava starch industries. The effect of several raw agro-industrial wastes and others nutrients on polygalacturonase production by W. anomalus, were evaluated, in a reference fermentation medium, using statistical designs, by batch culture. The ability of the cell-free supernatant to extract cassava starch was evaluated. Lemon peel was the best inducer for the production of PGase. Statistical analysis of the data showed that lemon peel, Mg+2 and PO4HK2 had significant effect on PGase production, and the others variables (yeast extract, Ca+2, Fe+2, amino acid and trace element solution) were no significant. PGase synthesis reached ~31 EUmL-1, in the OFM (glucose, lemon peel, urea, vitamins, KH2PO4 and MgSO4), after 12 h of culture, at a lab scale bioreactor. PGase of W. anomalus, was able to disintegrate cassava tuber tissue, and the starch granules contained within the cells were released into the reaction medium. Lemon peel can be used as inducer for PGase production by W. anomalus, in a low cost culture medium, appropriate for the production of the enzyme at large scale. |
| publishDate |
2020 |
| dc.date.none.fl_str_mv |
2020-06 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/142914 Maidana, Silvana Andrea; Esteche, Vanesa Paola; Hours, Roque Alberto; Brumovsky, Luis Alberto; Martos, María Alicia; Use of agro-industrial wastes for the production of a wild yeast enzyme with disintegration activity on plant tissues; Instituto de Tecnologia do Paraná; Brazilian Archives of Biology and Technology; 63; 6-2020; 1-13 1516-8913 1678-4324 CONICET Digital CONICET |
| url |
http://hdl.handle.net/11336/142914 |
| identifier_str_mv |
Maidana, Silvana Andrea; Esteche, Vanesa Paola; Hours, Roque Alberto; Brumovsky, Luis Alberto; Martos, María Alicia; Use of agro-industrial wastes for the production of a wild yeast enzyme with disintegration activity on plant tissues; Instituto de Tecnologia do Paraná; Brazilian Archives of Biology and Technology; 63; 6-2020; 1-13 1516-8913 1678-4324 CONICET Digital CONICET |
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eng |
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eng |
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info:eu-repo/semantics/altIdentifier/url/https://www.scielo.br/j/babt/a/SRfPks575Vskdx9wBTG5jhy/?lang=en |
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openAccess |
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application/pdf application/pdf application/pdf application/pdf application/pdf |
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Instituto de Tecnologia do Paraná |
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Instituto de Tecnologia do Paraná |
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reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
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Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
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dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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