Effects of fructose-induced metabolic syndrome on rat skeletal cells and tissue, and their responses to metformin treatment

Autores
Felice, Juan Ignacio; Schurman, León; McCarthy, Antonio Desmond; Sedlinsky, Claudia; Aguirre, José Ignacio; Cortizo, Ana María
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Aims: Deleterious effects of metabolic syndrome (MS) on bone are still controversial. In this study we evaluated the effects of a fructose-induced MS, and/or an oral treatment with metformin on the osteogenic potential of bone marrow mesenchymal stromal cells (MSC), as well as on bone formation and architecture. Methods: 32 male 8 week-old Wistar rats were assigned to four groups: control (C), control plus oral metformin (CM), rats receiving 10% fructose in drinking water (FRD), and FRD plus metformin (FRDM). Samples were collected to measure blood parameters, and to perform pQCT analysis and static and dynamic histomorphometry. MSC were isolated to determine their osteogenic potential. Results: Metformin improved blood parameters in FRDM rats. pQCTand static and dynamic histomorphometry showed no significant differences in trabecular and cortical bone parameters among groups. FRD reduced TRAP expression and osteocyte density in trabecular bone and metformin only normalized osteocyte density. FRD decreased the osteogenic potential of MSC and metformin administration could revert some of these parameters. Conclusions: FRD-induced MS shows reduction in MSC osteogenic potential, in osteocyte density and in TRAP activity. Oral metformin treatment was able to prevent trabecular osteocyte loss and the reduction in extracellular mineralization induced by FRD-induced MS.
Laboratorio de Investigación en Osteopatías y Metabolismo Mineral
Materia
Biología
Metabolic syndrome
Fructose
Bone architecture
Osteoblasts
Metformin
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/104013

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oai_identifier_str oai:sedici.unlp.edu.ar:10915/104013
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network_name_str SEDICI (UNLP)
spelling Effects of fructose-induced metabolic syndrome on rat skeletal cells and tissue, and their responses to metformin treatmentFelice, Juan IgnacioSchurman, LeónMcCarthy, Antonio DesmondSedlinsky, ClaudiaAguirre, José IgnacioCortizo, Ana MaríaBiologíaMetabolic syndromeFructoseBone architectureOsteoblastsMetforminAims: Deleterious effects of metabolic syndrome (MS) on bone are still controversial. In this study we evaluated the effects of a fructose-induced MS, and/or an oral treatment with metformin on the osteogenic potential of bone marrow mesenchymal stromal cells (MSC), as well as on bone formation and architecture. Methods: 32 male 8 week-old Wistar rats were assigned to four groups: control (C), control plus oral metformin (CM), rats receiving 10% fructose in drinking water (FRD), and FRD plus metformin (FRDM). Samples were collected to measure blood parameters, and to perform pQCT analysis and static and dynamic histomorphometry. MSC were isolated to determine their osteogenic potential. Results: Metformin improved blood parameters in FRDM rats. pQCTand static and dynamic histomorphometry showed no significant differences in trabecular and cortical bone parameters among groups. FRD reduced TRAP expression and osteocyte density in trabecular bone and metformin only normalized osteocyte density. FRD decreased the osteogenic potential of MSC and metformin administration could revert some of these parameters. Conclusions: FRD-induced MS shows reduction in MSC osteogenic potential, in osteocyte density and in TRAP activity. Oral metformin treatment was able to prevent trabecular osteocyte loss and the reduction in extracellular mineralization induced by FRD-induced MS.Laboratorio de Investigación en Osteopatías y Metabolismo Mineral2017info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf202-213http://sedici.unlp.edu.ar/handle/10915/104013enginfo:eu-repo/semantics/altIdentifier/issn/0168-8227info:eu-repo/semantics/altIdentifier/doi/10.1016/j.diabres.2017.02.011info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-03T10:54:53Zoai:sedici.unlp.edu.ar:10915/104013Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-03 10:54:54.177SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Effects of fructose-induced metabolic syndrome on rat skeletal cells and tissue, and their responses to metformin treatment
title Effects of fructose-induced metabolic syndrome on rat skeletal cells and tissue, and their responses to metformin treatment
spellingShingle Effects of fructose-induced metabolic syndrome on rat skeletal cells and tissue, and their responses to metformin treatment
Felice, Juan Ignacio
Biología
Metabolic syndrome
Fructose
Bone architecture
Osteoblasts
Metformin
title_short Effects of fructose-induced metabolic syndrome on rat skeletal cells and tissue, and their responses to metformin treatment
title_full Effects of fructose-induced metabolic syndrome on rat skeletal cells and tissue, and their responses to metformin treatment
title_fullStr Effects of fructose-induced metabolic syndrome on rat skeletal cells and tissue, and their responses to metformin treatment
title_full_unstemmed Effects of fructose-induced metabolic syndrome on rat skeletal cells and tissue, and their responses to metformin treatment
title_sort Effects of fructose-induced metabolic syndrome on rat skeletal cells and tissue, and their responses to metformin treatment
dc.creator.none.fl_str_mv Felice, Juan Ignacio
Schurman, León
McCarthy, Antonio Desmond
Sedlinsky, Claudia
Aguirre, José Ignacio
Cortizo, Ana María
author Felice, Juan Ignacio
author_facet Felice, Juan Ignacio
Schurman, León
McCarthy, Antonio Desmond
Sedlinsky, Claudia
Aguirre, José Ignacio
Cortizo, Ana María
author_role author
author2 Schurman, León
McCarthy, Antonio Desmond
Sedlinsky, Claudia
Aguirre, José Ignacio
Cortizo, Ana María
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Biología
Metabolic syndrome
Fructose
Bone architecture
Osteoblasts
Metformin
topic Biología
Metabolic syndrome
Fructose
Bone architecture
Osteoblasts
Metformin
dc.description.none.fl_txt_mv Aims: Deleterious effects of metabolic syndrome (MS) on bone are still controversial. In this study we evaluated the effects of a fructose-induced MS, and/or an oral treatment with metformin on the osteogenic potential of bone marrow mesenchymal stromal cells (MSC), as well as on bone formation and architecture. Methods: 32 male 8 week-old Wistar rats were assigned to four groups: control (C), control plus oral metformin (CM), rats receiving 10% fructose in drinking water (FRD), and FRD plus metformin (FRDM). Samples were collected to measure blood parameters, and to perform pQCT analysis and static and dynamic histomorphometry. MSC were isolated to determine their osteogenic potential. Results: Metformin improved blood parameters in FRDM rats. pQCTand static and dynamic histomorphometry showed no significant differences in trabecular and cortical bone parameters among groups. FRD reduced TRAP expression and osteocyte density in trabecular bone and metformin only normalized osteocyte density. FRD decreased the osteogenic potential of MSC and metformin administration could revert some of these parameters. Conclusions: FRD-induced MS shows reduction in MSC osteogenic potential, in osteocyte density and in TRAP activity. Oral metformin treatment was able to prevent trabecular osteocyte loss and the reduction in extracellular mineralization induced by FRD-induced MS.
Laboratorio de Investigación en Osteopatías y Metabolismo Mineral
description Aims: Deleterious effects of metabolic syndrome (MS) on bone are still controversial. In this study we evaluated the effects of a fructose-induced MS, and/or an oral treatment with metformin on the osteogenic potential of bone marrow mesenchymal stromal cells (MSC), as well as on bone formation and architecture. Methods: 32 male 8 week-old Wistar rats were assigned to four groups: control (C), control plus oral metformin (CM), rats receiving 10% fructose in drinking water (FRD), and FRD plus metformin (FRDM). Samples were collected to measure blood parameters, and to perform pQCT analysis and static and dynamic histomorphometry. MSC were isolated to determine their osteogenic potential. Results: Metformin improved blood parameters in FRDM rats. pQCTand static and dynamic histomorphometry showed no significant differences in trabecular and cortical bone parameters among groups. FRD reduced TRAP expression and osteocyte density in trabecular bone and metformin only normalized osteocyte density. FRD decreased the osteogenic potential of MSC and metformin administration could revert some of these parameters. Conclusions: FRD-induced MS shows reduction in MSC osteogenic potential, in osteocyte density and in TRAP activity. Oral metformin treatment was able to prevent trabecular osteocyte loss and the reduction in extracellular mineralization induced by FRD-induced MS.
publishDate 2017
dc.date.none.fl_str_mv 2017
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/104013
url http://sedici.unlp.edu.ar/handle/10915/104013
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/0168-8227
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.diabres.2017.02.011
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
202-213
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
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