Effects of fructose-induced metabolic syndrome on rat skeletal cells and tissue, and their responses to metformin treatment
- Autores
- Felice, Juan Ignacio; Schurman, León; McCarthy, Antonio Desmond; Sedlinsky, Claudia; Aguirre, José Ignacio; Cortizo, Ana María
- Año de publicación
- 2017
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Aims: Deleterious effects of metabolic syndrome (MS) on bone are still controversial. In this study we evaluated the effects of a fructose-induced MS, and/or an oral treatment with metformin on the osteogenic potential of bone marrow mesenchymal stromal cells (MSC), as well as on bone formation and architecture. Methods: 32 male 8 week-old Wistar rats were assigned to four groups: control (C), control plus oral metformin (CM), rats receiving 10% fructose in drinking water (FRD), and FRD plus metformin (FRDM). Samples were collected to measure blood parameters, and to perform pQCT analysis and static and dynamic histomorphometry. MSC were isolated to determine their osteogenic potential. Results: Metformin improved blood parameters in FRDM rats. pQCTand static and dynamic histomorphometry showed no significant differences in trabecular and cortical bone parameters among groups. FRD reduced TRAP expression and osteocyte density in trabecular bone and metformin only normalized osteocyte density. FRD decreased the osteogenic potential of MSC and metformin administration could revert some of these parameters. Conclusions: FRD-induced MS shows reduction in MSC osteogenic potential, in osteocyte density and in TRAP activity. Oral metformin treatment was able to prevent trabecular osteocyte loss and the reduction in extracellular mineralization induced by FRD-induced MS.
Laboratorio de Investigación en Osteopatías y Metabolismo Mineral - Materia
-
Biología
Metabolic syndrome
Fructose
Bone architecture
Osteoblasts
Metformin - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by-nc-sa/4.0/
- Repositorio
- Institución
- Universidad Nacional de La Plata
- OAI Identificador
- oai:sedici.unlp.edu.ar:10915/104013
Ver los metadatos del registro completo
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Effects of fructose-induced metabolic syndrome on rat skeletal cells and tissue, and their responses to metformin treatmentFelice, Juan IgnacioSchurman, LeónMcCarthy, Antonio DesmondSedlinsky, ClaudiaAguirre, José IgnacioCortizo, Ana MaríaBiologíaMetabolic syndromeFructoseBone architectureOsteoblastsMetforminAims: Deleterious effects of metabolic syndrome (MS) on bone are still controversial. In this study we evaluated the effects of a fructose-induced MS, and/or an oral treatment with metformin on the osteogenic potential of bone marrow mesenchymal stromal cells (MSC), as well as on bone formation and architecture. Methods: 32 male 8 week-old Wistar rats were assigned to four groups: control (C), control plus oral metformin (CM), rats receiving 10% fructose in drinking water (FRD), and FRD plus metformin (FRDM). Samples were collected to measure blood parameters, and to perform pQCT analysis and static and dynamic histomorphometry. MSC were isolated to determine their osteogenic potential. Results: Metformin improved blood parameters in FRDM rats. pQCTand static and dynamic histomorphometry showed no significant differences in trabecular and cortical bone parameters among groups. FRD reduced TRAP expression and osteocyte density in trabecular bone and metformin only normalized osteocyte density. FRD decreased the osteogenic potential of MSC and metformin administration could revert some of these parameters. Conclusions: FRD-induced MS shows reduction in MSC osteogenic potential, in osteocyte density and in TRAP activity. Oral metformin treatment was able to prevent trabecular osteocyte loss and the reduction in extracellular mineralization induced by FRD-induced MS.Laboratorio de Investigación en Osteopatías y Metabolismo Mineral2017info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf202-213http://sedici.unlp.edu.ar/handle/10915/104013enginfo:eu-repo/semantics/altIdentifier/issn/0168-8227info:eu-repo/semantics/altIdentifier/doi/10.1016/j.diabres.2017.02.011info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-03T10:54:53Zoai:sedici.unlp.edu.ar:10915/104013Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-03 10:54:54.177SEDICI (UNLP) - Universidad Nacional de La Platafalse |
dc.title.none.fl_str_mv |
Effects of fructose-induced metabolic syndrome on rat skeletal cells and tissue, and their responses to metformin treatment |
title |
Effects of fructose-induced metabolic syndrome on rat skeletal cells and tissue, and their responses to metformin treatment |
spellingShingle |
Effects of fructose-induced metabolic syndrome on rat skeletal cells and tissue, and their responses to metformin treatment Felice, Juan Ignacio Biología Metabolic syndrome Fructose Bone architecture Osteoblasts Metformin |
title_short |
Effects of fructose-induced metabolic syndrome on rat skeletal cells and tissue, and their responses to metformin treatment |
title_full |
Effects of fructose-induced metabolic syndrome on rat skeletal cells and tissue, and their responses to metformin treatment |
title_fullStr |
Effects of fructose-induced metabolic syndrome on rat skeletal cells and tissue, and their responses to metformin treatment |
title_full_unstemmed |
Effects of fructose-induced metabolic syndrome on rat skeletal cells and tissue, and their responses to metformin treatment |
title_sort |
Effects of fructose-induced metabolic syndrome on rat skeletal cells and tissue, and their responses to metformin treatment |
dc.creator.none.fl_str_mv |
Felice, Juan Ignacio Schurman, León McCarthy, Antonio Desmond Sedlinsky, Claudia Aguirre, José Ignacio Cortizo, Ana María |
author |
Felice, Juan Ignacio |
author_facet |
Felice, Juan Ignacio Schurman, León McCarthy, Antonio Desmond Sedlinsky, Claudia Aguirre, José Ignacio Cortizo, Ana María |
author_role |
author |
author2 |
Schurman, León McCarthy, Antonio Desmond Sedlinsky, Claudia Aguirre, José Ignacio Cortizo, Ana María |
author2_role |
author author author author author |
dc.subject.none.fl_str_mv |
Biología Metabolic syndrome Fructose Bone architecture Osteoblasts Metformin |
topic |
Biología Metabolic syndrome Fructose Bone architecture Osteoblasts Metformin |
dc.description.none.fl_txt_mv |
Aims: Deleterious effects of metabolic syndrome (MS) on bone are still controversial. In this study we evaluated the effects of a fructose-induced MS, and/or an oral treatment with metformin on the osteogenic potential of bone marrow mesenchymal stromal cells (MSC), as well as on bone formation and architecture. Methods: 32 male 8 week-old Wistar rats were assigned to four groups: control (C), control plus oral metformin (CM), rats receiving 10% fructose in drinking water (FRD), and FRD plus metformin (FRDM). Samples were collected to measure blood parameters, and to perform pQCT analysis and static and dynamic histomorphometry. MSC were isolated to determine their osteogenic potential. Results: Metformin improved blood parameters in FRDM rats. pQCTand static and dynamic histomorphometry showed no significant differences in trabecular and cortical bone parameters among groups. FRD reduced TRAP expression and osteocyte density in trabecular bone and metformin only normalized osteocyte density. FRD decreased the osteogenic potential of MSC and metformin administration could revert some of these parameters. Conclusions: FRD-induced MS shows reduction in MSC osteogenic potential, in osteocyte density and in TRAP activity. Oral metformin treatment was able to prevent trabecular osteocyte loss and the reduction in extracellular mineralization induced by FRD-induced MS. Laboratorio de Investigación en Osteopatías y Metabolismo Mineral |
description |
Aims: Deleterious effects of metabolic syndrome (MS) on bone are still controversial. In this study we evaluated the effects of a fructose-induced MS, and/or an oral treatment with metformin on the osteogenic potential of bone marrow mesenchymal stromal cells (MSC), as well as on bone formation and architecture. Methods: 32 male 8 week-old Wistar rats were assigned to four groups: control (C), control plus oral metformin (CM), rats receiving 10% fructose in drinking water (FRD), and FRD plus metformin (FRDM). Samples were collected to measure blood parameters, and to perform pQCT analysis and static and dynamic histomorphometry. MSC were isolated to determine their osteogenic potential. Results: Metformin improved blood parameters in FRDM rats. pQCTand static and dynamic histomorphometry showed no significant differences in trabecular and cortical bone parameters among groups. FRD reduced TRAP expression and osteocyte density in trabecular bone and metformin only normalized osteocyte density. FRD decreased the osteogenic potential of MSC and metformin administration could revert some of these parameters. Conclusions: FRD-induced MS shows reduction in MSC osteogenic potential, in osteocyte density and in TRAP activity. Oral metformin treatment was able to prevent trabecular osteocyte loss and the reduction in extracellular mineralization induced by FRD-induced MS. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Articulo http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
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http://sedici.unlp.edu.ar/handle/10915/104013 |
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eng |
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eng |
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info:eu-repo/semantics/altIdentifier/issn/0168-8227 info:eu-repo/semantics/altIdentifier/doi/10.1016/j.diabres.2017.02.011 |
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info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
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openAccess |
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http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
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