Effects of fructose-induced metabolic syndrome on rat skeletal cells and tissue, and their responses to metformin treatment
- Autores
- Felice, Juan Ignacio; Schurman, León; McCarthy, Antonio Desmond; Sedlinsky, Claudia; Aguirre, José Ignacio; Cortizo, Ana María
- Año de publicación
- 2017
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Aims: Deleterious effects of metabolic syndrome (MS) on bone are still controversial. In this study we evaluated the effects of a fructose-induced MS, and/or an oral treatment with metformin on the osteogenic potential of bone marrow mesenchymal stromal cells (MSC), as well as on bone formation and architecture. Methods: 32 male 8 week-old Wistar rats were assigned to four groups: control (C), control plus oral metformin (CM), rats receiving 10% fructose in drinking water (FRD), and FRD plus metformin (FRDM). Samples were collected to measure blood parameters, and to perform pQCT analysis and static and dynamic histomorphometry. MSC were isolated to determine their osteogenic potential. Results: Metformin improved blood parameters in FRDM rats. pQCTand static and dynamic histomorphometry showed no significant differences in trabecular and cortical bone parameters among groups. FRD reduced TRAP expression and osteocyte density in trabecular bone and metformin only normalized osteocyte density. FRD decreased the osteogenic potential of MSC and metformin administration could revert some of these parameters. Conclusions: FRD-induced MS shows reduction in MSC osteogenic potential, in osteocyte density and in TRAP activity. Oral metformin treatment was able to prevent trabecular osteocyte loss and the reduction in extracellular mineralization induced by FRD-induced MS.
- Materia
-
Ciencias Biológicas
Metabolic syndrome
Fructose
Bone architecture
Osteoblasts
Metformin - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by-nc-sa/4.0/
- Repositorio
- Institución
- Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
- OAI Identificador
- oai:digital.cic.gba.gob.ar:11746/11325
Ver los metadatos del registro completo
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Effects of fructose-induced metabolic syndrome on rat skeletal cells and tissue, and their responses to metformin treatmentFelice, Juan IgnacioSchurman, LeónMcCarthy, Antonio DesmondSedlinsky, ClaudiaAguirre, José IgnacioCortizo, Ana MaríaCiencias BiológicasMetabolic syndromeFructoseBone architectureOsteoblastsMetforminAims: Deleterious effects of metabolic syndrome (MS) on bone are still controversial. In this study we evaluated the effects of a fructose-induced MS, and/or an oral treatment with metformin on the osteogenic potential of bone marrow mesenchymal stromal cells (MSC), as well as on bone formation and architecture. Methods: 32 male 8 week-old Wistar rats were assigned to four groups: control (C), control plus oral metformin (CM), rats receiving 10% fructose in drinking water (FRD), and FRD plus metformin (FRDM). Samples were collected to measure blood parameters, and to perform pQCT analysis and static and dynamic histomorphometry. MSC were isolated to determine their osteogenic potential. Results: Metformin improved blood parameters in FRDM rats. pQCTand static and dynamic histomorphometry showed no significant differences in trabecular and cortical bone parameters among groups. FRD reduced TRAP expression and osteocyte density in trabecular bone and metformin only normalized osteocyte density. FRD decreased the osteogenic potential of MSC and metformin administration could revert some of these parameters. Conclusions: FRD-induced MS shows reduction in MSC osteogenic potential, in osteocyte density and in TRAP activity. Oral metformin treatment was able to prevent trabecular osteocyte loss and the reduction in extracellular mineralization induced by FRD-induced MS.2017info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://digital.cic.gba.gob.ar/handle/11746/11325enginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.diabres.2017.02.011info:eu-repo/semantics/altIdentifier/issn/0168-8227info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/reponame:CIC Digital (CICBA)instname:Comisión de Investigaciones Científicas de la Provincia de Buenos Airesinstacron:CICBA2025-09-04T09:43:53Zoai:digital.cic.gba.gob.ar:11746/11325Institucionalhttp://digital.cic.gba.gob.arOrganismo científico-tecnológicoNo correspondehttp://digital.cic.gba.gob.ar/oai/snrdmarisa.degiusti@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:94412025-09-04 09:43:54.54CIC Digital (CICBA) - Comisión de Investigaciones Científicas de la Provincia de Buenos Airesfalse |
dc.title.none.fl_str_mv |
Effects of fructose-induced metabolic syndrome on rat skeletal cells and tissue, and their responses to metformin treatment |
title |
Effects of fructose-induced metabolic syndrome on rat skeletal cells and tissue, and their responses to metformin treatment |
spellingShingle |
Effects of fructose-induced metabolic syndrome on rat skeletal cells and tissue, and their responses to metformin treatment Felice, Juan Ignacio Ciencias Biológicas Metabolic syndrome Fructose Bone architecture Osteoblasts Metformin |
title_short |
Effects of fructose-induced metabolic syndrome on rat skeletal cells and tissue, and their responses to metformin treatment |
title_full |
Effects of fructose-induced metabolic syndrome on rat skeletal cells and tissue, and their responses to metformin treatment |
title_fullStr |
Effects of fructose-induced metabolic syndrome on rat skeletal cells and tissue, and their responses to metformin treatment |
title_full_unstemmed |
Effects of fructose-induced metabolic syndrome on rat skeletal cells and tissue, and their responses to metformin treatment |
title_sort |
Effects of fructose-induced metabolic syndrome on rat skeletal cells and tissue, and their responses to metformin treatment |
dc.creator.none.fl_str_mv |
Felice, Juan Ignacio Schurman, León McCarthy, Antonio Desmond Sedlinsky, Claudia Aguirre, José Ignacio Cortizo, Ana María |
author |
Felice, Juan Ignacio |
author_facet |
Felice, Juan Ignacio Schurman, León McCarthy, Antonio Desmond Sedlinsky, Claudia Aguirre, José Ignacio Cortizo, Ana María |
author_role |
author |
author2 |
Schurman, León McCarthy, Antonio Desmond Sedlinsky, Claudia Aguirre, José Ignacio Cortizo, Ana María |
author2_role |
author author author author author |
dc.subject.none.fl_str_mv |
Ciencias Biológicas Metabolic syndrome Fructose Bone architecture Osteoblasts Metformin |
topic |
Ciencias Biológicas Metabolic syndrome Fructose Bone architecture Osteoblasts Metformin |
dc.description.none.fl_txt_mv |
Aims: Deleterious effects of metabolic syndrome (MS) on bone are still controversial. In this study we evaluated the effects of a fructose-induced MS, and/or an oral treatment with metformin on the osteogenic potential of bone marrow mesenchymal stromal cells (MSC), as well as on bone formation and architecture. Methods: 32 male 8 week-old Wistar rats were assigned to four groups: control (C), control plus oral metformin (CM), rats receiving 10% fructose in drinking water (FRD), and FRD plus metformin (FRDM). Samples were collected to measure blood parameters, and to perform pQCT analysis and static and dynamic histomorphometry. MSC were isolated to determine their osteogenic potential. Results: Metformin improved blood parameters in FRDM rats. pQCTand static and dynamic histomorphometry showed no significant differences in trabecular and cortical bone parameters among groups. FRD reduced TRAP expression and osteocyte density in trabecular bone and metformin only normalized osteocyte density. FRD decreased the osteogenic potential of MSC and metformin administration could revert some of these parameters. Conclusions: FRD-induced MS shows reduction in MSC osteogenic potential, in osteocyte density and in TRAP activity. Oral metformin treatment was able to prevent trabecular osteocyte loss and the reduction in extracellular mineralization induced by FRD-induced MS. |
description |
Aims: Deleterious effects of metabolic syndrome (MS) on bone are still controversial. In this study we evaluated the effects of a fructose-induced MS, and/or an oral treatment with metformin on the osteogenic potential of bone marrow mesenchymal stromal cells (MSC), as well as on bone formation and architecture. Methods: 32 male 8 week-old Wistar rats were assigned to four groups: control (C), control plus oral metformin (CM), rats receiving 10% fructose in drinking water (FRD), and FRD plus metformin (FRDM). Samples were collected to measure blood parameters, and to perform pQCT analysis and static and dynamic histomorphometry. MSC were isolated to determine their osteogenic potential. Results: Metformin improved blood parameters in FRDM rats. pQCTand static and dynamic histomorphometry showed no significant differences in trabecular and cortical bone parameters among groups. FRD reduced TRAP expression and osteocyte density in trabecular bone and metformin only normalized osteocyte density. FRD decreased the osteogenic potential of MSC and metformin administration could revert some of these parameters. Conclusions: FRD-induced MS shows reduction in MSC osteogenic potential, in osteocyte density and in TRAP activity. Oral metformin treatment was able to prevent trabecular osteocyte loss and the reduction in extracellular mineralization induced by FRD-induced MS. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
https://digital.cic.gba.gob.ar/handle/11746/11325 |
url |
https://digital.cic.gba.gob.ar/handle/11746/11325 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.diabres.2017.02.011 info:eu-repo/semantics/altIdentifier/issn/0168-8227 |
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info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ |
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openAccess |
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http://creativecommons.org/licenses/by-nc-sa/4.0/ |
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application/pdf |
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CIC Digital (CICBA) - Comisión de Investigaciones Científicas de la Provincia de Buenos Aires |
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