<i>Neospora caninum</i> NC-6 Argentina induces fetopathy in both serologically positive and negative experimentally inoculated pregnant dams

Autores
Bacigalupe, Diana Raquel; Basso, Walter Ubaldo; Caspe, S. G.; Moré, Gastón Andrés; Lischinsky, L.; Gos, María Laura; Leunda, María Rosa; Campero, Lucía María; Moore, Dadín Prando; Schares, Gereon; Campero, Carlos Manuel; Venturini, María Cecilia
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Neospora caninum infection is a major cause of abortion in cattle. The objectives of this study were to genetically characterize the N. caninum NC-6 Argentina isolate using a multilocus microsatellite analysis approach and to study its biological behavior by experimental inoculations into seronegative and seropositive pregnant cattle, evaluating the humoral and cellular immune response elicited and the occurrence of transplacental transmission and fetopathy. Pregnant cows (65 days of gestation) seropositive and seronegative to N. caninum were intravenously inoculated with tachyzoites of the NC-6 Argentina N. caninum strain and slaughtered at 108 ± 2 days of gestation. Serum samples were analyzed for N. caninum antibodies by indirect fluorescent antibody test. The cellular immune response was analyzed by detection of gamma interferon (γIFN) production in blood cells. Tissue samples from dams, fetuses, and placental cotyledons were processed by histopathological and immunohistochemical techniques and examined for N. caninum DNA by PCR. Positive DNA samples were further analyzed by multilocus microsatellite typing for N. caninum. Inoculated animals had significantly higher N. caninum antibody titers and γIFN production than control animals. One seropositive inoculated cow aborted, one seronegative cow had a non-viable fetus, and the remaining fetuses from the experimentally inoculated dams had histopathologic lesions. The PCR was positive in 3/4 fetuses from seronegative inoculated cows and in 2/3 fetuses from seropositive inoculated cows. Multilocus microsatellite analysis revealed that the N. caninum DNA present in fetuses and placentas had an identical pattern to NC-6 Argentina strain. The NC-6 Argentina strain proved to be able to cross the placenta and to induce fetopathy in both the seropositive and seronegative dams.
Laboratorio de Inmunoparasitología
Facultad de Ciencias Veterinarias
Materia
Veterinaria
Cellular Immune Response
Brucellosis
Bovine Viral Diarrhea Virus
Seropositive Animal
Transplacental Transmission
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/137194
Acceder
id SEDICI_ccf48e422abd8c9e56cc7d5953be4b6c
oai_identifier_str oai:sedici.unlp.edu.ar:10915/137194
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling <i>Neospora caninum</i> NC-6 Argentina induces fetopathy in both serologically positive and negative experimentally inoculated pregnant damsBacigalupe, Diana RaquelBasso, Walter UbaldoCaspe, S. G.Moré, Gastón AndrésLischinsky, L.Gos, María LauraLeunda, María RosaCampero, Lucía MaríaMoore, Dadín PrandoSchares, GereonCampero, Carlos ManuelVenturini, María CeciliaVeterinariaCellular Immune ResponseBrucellosisBovine Viral Diarrhea VirusSeropositive AnimalTransplacental Transmission<i>Neospora caninum</i> infection is a major cause of abortion in cattle. The objectives of this study were to genetically characterize the <i>N. caninum</i> NC-6 Argentina isolate using a multilocus microsatellite analysis approach and to study its biological behavior by experimental inoculations into seronegative and seropositive pregnant cattle, evaluating the humoral and cellular immune response elicited and the occurrence of transplacental transmission and fetopathy. Pregnant cows (65 days of gestation) seropositive and seronegative to <i>N. caninum</i> were intravenously inoculated with tachyzoites of the NC-6 Argentina <i>N. caninum</i> strain and slaughtered at 108 ± 2 days of gestation. Serum samples were analyzed for <i>N. caninum</i> antibodies by indirect fluorescent antibody test. The cellular immune response was analyzed by detection of gamma interferon (γIFN) production in blood cells. Tissue samples from dams, fetuses, and placental cotyledons were processed by histopathological and immunohistochemical techniques and examined for <i>N. caninum</i> DNA by PCR. Positive DNA samples were further analyzed by multilocus microsatellite typing for <i>N. caninum</i>. Inoculated animals had significantly higher <i>N. caninum</i> antibody titers and γIFN production than control animals. One seropositive inoculated cow aborted, one seronegative cow had a non-viable fetus, and the remaining fetuses from the experimentally inoculated dams had histopathologic lesions. The PCR was positive in 3/4 fetuses from seronegative inoculated cows and in 2/3 fetuses from seropositive inoculated cows. Multilocus microsatellite analysis revealed that the <i>N. caninum</i> DNA present in fetuses and placentas had an identical pattern to NC-6 Argentina strain. The NC-6 Argentina strain proved to be able to cross the placenta and to induce fetopathy in both the seropositive and seronegative dams.Laboratorio de InmunoparasitologíaFacultad de Ciencias Veterinarias2013-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf2585-2592http://sedici.unlp.edu.ar/handle/10915/137194enginfo:eu-repo/semantics/altIdentifier/issn/1432-1955info:eu-repo/semantics/altIdentifier/issn/0932-0113info:eu-repo/semantics/altIdentifier/doi/10.1007/s00436-013-3424-1info:eu-repo/semantics/altIdentifier/pmid/23666227info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-10-22T17:12:57Zoai:sedici.unlp.edu.ar:10915/137194Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-10-22 17:12:57.398SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv <i>Neospora caninum</i> NC-6 Argentina induces fetopathy in both serologically positive and negative experimentally inoculated pregnant dams
title <i>Neospora caninum</i> NC-6 Argentina induces fetopathy in both serologically positive and negative experimentally inoculated pregnant dams
spellingShingle <i>Neospora caninum</i> NC-6 Argentina induces fetopathy in both serologically positive and negative experimentally inoculated pregnant dams
Bacigalupe, Diana Raquel
Veterinaria
Cellular Immune Response
Brucellosis
Bovine Viral Diarrhea Virus
Seropositive Animal
Transplacental Transmission
title_short <i>Neospora caninum</i> NC-6 Argentina induces fetopathy in both serologically positive and negative experimentally inoculated pregnant dams
title_full <i>Neospora caninum</i> NC-6 Argentina induces fetopathy in both serologically positive and negative experimentally inoculated pregnant dams
title_fullStr <i>Neospora caninum</i> NC-6 Argentina induces fetopathy in both serologically positive and negative experimentally inoculated pregnant dams
title_full_unstemmed <i>Neospora caninum</i> NC-6 Argentina induces fetopathy in both serologically positive and negative experimentally inoculated pregnant dams
title_sort <i>Neospora caninum</i> NC-6 Argentina induces fetopathy in both serologically positive and negative experimentally inoculated pregnant dams
dc.creator.none.fl_str_mv Bacigalupe, Diana Raquel
Basso, Walter Ubaldo
Caspe, S. G.
Moré, Gastón Andrés
Lischinsky, L.
Gos, María Laura
Leunda, María Rosa
Campero, Lucía María
Moore, Dadín Prando
Schares, Gereon
Campero, Carlos Manuel
Venturini, María Cecilia
author Bacigalupe, Diana Raquel
author_facet Bacigalupe, Diana Raquel
Basso, Walter Ubaldo
Caspe, S. G.
Moré, Gastón Andrés
Lischinsky, L.
Gos, María Laura
Leunda, María Rosa
Campero, Lucía María
Moore, Dadín Prando
Schares, Gereon
Campero, Carlos Manuel
Venturini, María Cecilia
author_role author
author2 Basso, Walter Ubaldo
Caspe, S. G.
Moré, Gastón Andrés
Lischinsky, L.
Gos, María Laura
Leunda, María Rosa
Campero, Lucía María
Moore, Dadín Prando
Schares, Gereon
Campero, Carlos Manuel
Venturini, María Cecilia
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Veterinaria
Cellular Immune Response
Brucellosis
Bovine Viral Diarrhea Virus
Seropositive Animal
Transplacental Transmission
topic Veterinaria
Cellular Immune Response
Brucellosis
Bovine Viral Diarrhea Virus
Seropositive Animal
Transplacental Transmission
dc.description.none.fl_txt_mv <i>Neospora caninum</i> infection is a major cause of abortion in cattle. The objectives of this study were to genetically characterize the <i>N. caninum</i> NC-6 Argentina isolate using a multilocus microsatellite analysis approach and to study its biological behavior by experimental inoculations into seronegative and seropositive pregnant cattle, evaluating the humoral and cellular immune response elicited and the occurrence of transplacental transmission and fetopathy. Pregnant cows (65 days of gestation) seropositive and seronegative to <i>N. caninum</i> were intravenously inoculated with tachyzoites of the NC-6 Argentina <i>N. caninum</i> strain and slaughtered at 108 ± 2 days of gestation. Serum samples were analyzed for <i>N. caninum</i> antibodies by indirect fluorescent antibody test. The cellular immune response was analyzed by detection of gamma interferon (γIFN) production in blood cells. Tissue samples from dams, fetuses, and placental cotyledons were processed by histopathological and immunohistochemical techniques and examined for <i>N. caninum</i> DNA by PCR. Positive DNA samples were further analyzed by multilocus microsatellite typing for <i>N. caninum</i>. Inoculated animals had significantly higher <i>N. caninum</i> antibody titers and γIFN production than control animals. One seropositive inoculated cow aborted, one seronegative cow had a non-viable fetus, and the remaining fetuses from the experimentally inoculated dams had histopathologic lesions. The PCR was positive in 3/4 fetuses from seronegative inoculated cows and in 2/3 fetuses from seropositive inoculated cows. Multilocus microsatellite analysis revealed that the <i>N. caninum</i> DNA present in fetuses and placentas had an identical pattern to NC-6 Argentina strain. The NC-6 Argentina strain proved to be able to cross the placenta and to induce fetopathy in both the seropositive and seronegative dams.
Laboratorio de Inmunoparasitología
Facultad de Ciencias Veterinarias
description <i>Neospora caninum</i> infection is a major cause of abortion in cattle. The objectives of this study were to genetically characterize the <i>N. caninum</i> NC-6 Argentina isolate using a multilocus microsatellite analysis approach and to study its biological behavior by experimental inoculations into seronegative and seropositive pregnant cattle, evaluating the humoral and cellular immune response elicited and the occurrence of transplacental transmission and fetopathy. Pregnant cows (65 days of gestation) seropositive and seronegative to <i>N. caninum</i> were intravenously inoculated with tachyzoites of the NC-6 Argentina <i>N. caninum</i> strain and slaughtered at 108 ± 2 days of gestation. Serum samples were analyzed for <i>N. caninum</i> antibodies by indirect fluorescent antibody test. The cellular immune response was analyzed by detection of gamma interferon (γIFN) production in blood cells. Tissue samples from dams, fetuses, and placental cotyledons were processed by histopathological and immunohistochemical techniques and examined for <i>N. caninum</i> DNA by PCR. Positive DNA samples were further analyzed by multilocus microsatellite typing for <i>N. caninum</i>. Inoculated animals had significantly higher <i>N. caninum</i> antibody titers and γIFN production than control animals. One seropositive inoculated cow aborted, one seronegative cow had a non-viable fetus, and the remaining fetuses from the experimentally inoculated dams had histopathologic lesions. The PCR was positive in 3/4 fetuses from seronegative inoculated cows and in 2/3 fetuses from seropositive inoculated cows. Multilocus microsatellite analysis revealed that the <i>N. caninum</i> DNA present in fetuses and placentas had an identical pattern to NC-6 Argentina strain. The NC-6 Argentina strain proved to be able to cross the placenta and to induce fetopathy in both the seropositive and seronegative dams.
publishDate 2013
dc.date.none.fl_str_mv 2013-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/137194
url http://sedici.unlp.edu.ar/handle/10915/137194
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/1432-1955
info:eu-repo/semantics/altIdentifier/issn/0932-0113
info:eu-repo/semantics/altIdentifier/doi/10.1007/s00436-013-3424-1
info:eu-repo/semantics/altIdentifier/pmid/23666227
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
dc.format.none.fl_str_mv application/pdf
2585-2592
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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