Diagnosis of Sarcocystis cruzi, Neospora caninum, and Toxoplasma gondii infections in cattle

Autores
Moré, Gastón Andrés; Basso, Walter Ubaldo; Bacigalupe, Diana Raquel; Venturini, María Cecilia; Venturini, María Lucila
Año de publicación
2008
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The aim of the study was to diagnose Sarcocystis sp. infections in cattle and to detect coinfections by Toxoplasma gondii and/or Neospora caninum. Blood, diaphragm, esophagus, and myocardium from 90 beef cattle from Argentina were collected. Histopathological, immunohistochemical, polymerase chain reaction assays, and direct microscopical examination were carried out. Sarcocysts from myocardium were measured and counted. Indirect fluorescent antibody test (IFAT) for the three protozoans was performed. Sarcocystis cruzi sarcocysts were found in 100% of myocardium samples. Sarcocysts per gram ranged from 8 to 380 with higher values found in adult cattle (p < 0.001). T. gondii and N. caninum were not detected by immunohistochemistry. T. gondii DNA was found in myocardium of 2/20 seropositive animals, while N. caninum DNA was not found. Antibodies against S. cruzi were detected in all samples, those against N. caninum in 73% and against T. gondii in 91% of the samples (IFAT titer ≥25). It is concluded that serology by IFAT is a suitable method to diagnose these protozoan infections due to its specific IgG detection; therefore, IFAT may be a useful tool to evaluate the impact of each protozoan infection in coinfected animals.
Facultad de Ciencias Veterinarias
Materia
Ciencias Veterinarias
Beef Cattle
Indirect Fluorescent Antibody Test
Adult Cattle
Sarcocystis Species
Seropositive Animal
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/136315
Acceder
id SEDICI_5bbe4cf0e86c00ad38b2bb484de6d685
oai_identifier_str oai:sedici.unlp.edu.ar:10915/136315
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Diagnosis of Sarcocystis cruzi, Neospora caninum, and Toxoplasma gondii infections in cattleMoré, Gastón AndrésBasso, Walter UbaldoBacigalupe, Diana RaquelVenturini, María CeciliaVenturini, María LucilaCiencias VeterinariasBeef CattleIndirect Fluorescent Antibody TestAdult CattleSarcocystis SpeciesSeropositive AnimalThe aim of the study was to diagnose Sarcocystis sp. infections in cattle and to detect coinfections by Toxoplasma gondii and/or Neospora caninum. Blood, diaphragm, esophagus, and myocardium from 90 beef cattle from Argentina were collected. Histopathological, immunohistochemical, polymerase chain reaction assays, and direct microscopical examination were carried out. Sarcocysts from myocardium were measured and counted. Indirect fluorescent antibody test (IFAT) for the three protozoans was performed. Sarcocystis cruzi sarcocysts were found in 100% of myocardium samples. Sarcocysts per gram ranged from 8 to 380 with higher values found in adult cattle (p < 0.001). T. gondii and N. caninum were not detected by immunohistochemistry. T. gondii DNA was found in myocardium of 2/20 seropositive animals, while N. caninum DNA was not found. Antibodies against S. cruzi were detected in all samples, those against N. caninum in 73% and against T. gondii in 91% of the samples (IFAT titer ≥25). It is concluded that serology by IFAT is a suitable method to diagnose these protozoan infections due to its specific IgG detection; therefore, IFAT may be a useful tool to evaluate the impact of each protozoan infection in coinfected animals.Facultad de Ciencias Veterinarias2008info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf671-675http://sedici.unlp.edu.ar/handle/10915/136315enginfo:eu-repo/semantics/altIdentifier/issn/0932-0113info:eu-repo/semantics/altIdentifier/issn/1432-1955info:eu-repo/semantics/altIdentifier/doi/10.1007/s00436-007-0810-6info:eu-repo/semantics/altIdentifier/pmid/18066600info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:32:21Zoai:sedici.unlp.edu.ar:10915/136315Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:32:22.238SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Diagnosis of Sarcocystis cruzi, Neospora caninum, and Toxoplasma gondii infections in cattle
title Diagnosis of Sarcocystis cruzi, Neospora caninum, and Toxoplasma gondii infections in cattle
spellingShingle Diagnosis of Sarcocystis cruzi, Neospora caninum, and Toxoplasma gondii infections in cattle
Moré, Gastón Andrés
Ciencias Veterinarias
Beef Cattle
Indirect Fluorescent Antibody Test
Adult Cattle
Sarcocystis Species
Seropositive Animal
title_short Diagnosis of Sarcocystis cruzi, Neospora caninum, and Toxoplasma gondii infections in cattle
title_full Diagnosis of Sarcocystis cruzi, Neospora caninum, and Toxoplasma gondii infections in cattle
title_fullStr Diagnosis of Sarcocystis cruzi, Neospora caninum, and Toxoplasma gondii infections in cattle
title_full_unstemmed Diagnosis of Sarcocystis cruzi, Neospora caninum, and Toxoplasma gondii infections in cattle
title_sort Diagnosis of Sarcocystis cruzi, Neospora caninum, and Toxoplasma gondii infections in cattle
dc.creator.none.fl_str_mv Moré, Gastón Andrés
Basso, Walter Ubaldo
Bacigalupe, Diana Raquel
Venturini, María Cecilia
Venturini, María Lucila
author Moré, Gastón Andrés
author_facet Moré, Gastón Andrés
Basso, Walter Ubaldo
Bacigalupe, Diana Raquel
Venturini, María Cecilia
Venturini, María Lucila
author_role author
author2 Basso, Walter Ubaldo
Bacigalupe, Diana Raquel
Venturini, María Cecilia
Venturini, María Lucila
author2_role author
author
author
author
dc.subject.none.fl_str_mv Ciencias Veterinarias
Beef Cattle
Indirect Fluorescent Antibody Test
Adult Cattle
Sarcocystis Species
Seropositive Animal
topic Ciencias Veterinarias
Beef Cattle
Indirect Fluorescent Antibody Test
Adult Cattle
Sarcocystis Species
Seropositive Animal
dc.description.none.fl_txt_mv The aim of the study was to diagnose Sarcocystis sp. infections in cattle and to detect coinfections by Toxoplasma gondii and/or Neospora caninum. Blood, diaphragm, esophagus, and myocardium from 90 beef cattle from Argentina were collected. Histopathological, immunohistochemical, polymerase chain reaction assays, and direct microscopical examination were carried out. Sarcocysts from myocardium were measured and counted. Indirect fluorescent antibody test (IFAT) for the three protozoans was performed. Sarcocystis cruzi sarcocysts were found in 100% of myocardium samples. Sarcocysts per gram ranged from 8 to 380 with higher values found in adult cattle (p < 0.001). T. gondii and N. caninum were not detected by immunohistochemistry. T. gondii DNA was found in myocardium of 2/20 seropositive animals, while N. caninum DNA was not found. Antibodies against S. cruzi were detected in all samples, those against N. caninum in 73% and against T. gondii in 91% of the samples (IFAT titer ≥25). It is concluded that serology by IFAT is a suitable method to diagnose these protozoan infections due to its specific IgG detection; therefore, IFAT may be a useful tool to evaluate the impact of each protozoan infection in coinfected animals.
Facultad de Ciencias Veterinarias
description The aim of the study was to diagnose Sarcocystis sp. infections in cattle and to detect coinfections by Toxoplasma gondii and/or Neospora caninum. Blood, diaphragm, esophagus, and myocardium from 90 beef cattle from Argentina were collected. Histopathological, immunohistochemical, polymerase chain reaction assays, and direct microscopical examination were carried out. Sarcocysts from myocardium were measured and counted. Indirect fluorescent antibody test (IFAT) for the three protozoans was performed. Sarcocystis cruzi sarcocysts were found in 100% of myocardium samples. Sarcocysts per gram ranged from 8 to 380 with higher values found in adult cattle (p < 0.001). T. gondii and N. caninum were not detected by immunohistochemistry. T. gondii DNA was found in myocardium of 2/20 seropositive animals, while N. caninum DNA was not found. Antibodies against S. cruzi were detected in all samples, those against N. caninum in 73% and against T. gondii in 91% of the samples (IFAT titer ≥25). It is concluded that serology by IFAT is a suitable method to diagnose these protozoan infections due to its specific IgG detection; therefore, IFAT may be a useful tool to evaluate the impact of each protozoan infection in coinfected animals.
publishDate 2008
dc.date.none.fl_str_mv 2008
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/136315
url http://sedici.unlp.edu.ar/handle/10915/136315
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/0932-0113
info:eu-repo/semantics/altIdentifier/issn/1432-1955
info:eu-repo/semantics/altIdentifier/doi/10.1007/s00436-007-0810-6
info:eu-repo/semantics/altIdentifier/pmid/18066600
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
671-675
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
_version_ 1844616203152130048
score 13.070432

Publicaciones similares