A conventional PCR technique to detect <i>Septoria tritici</i> in wheat seeds
- Autores
- Consolo, Verónica Fabiana; Albani, Clara María; Berón, Corina M.; Salerno, Graciela Lidia; Cordo, Cristina Alicia
- Año de publicación
- 2009
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- A PCR assay was developed for detection of wheat seed naturally contaminated with Septoria tritici. S. tritici specific primers were derived from strict alignment of ITS and α-tubulin sequences of the pathogen. The specificity of four sets of synthesised oligonucleotide pairs (A, B, C and D) were tested using isolates from S. tritici, other selected fungi and wheat seeds. A single DNA fragment was amplified from S. tritici isolates with all primer pairs, whereas no product was generated from other DNA sources. S. tritici was also detected in wheat seed lots collected from plants with variable pycnidial coverage on the upper two leaves. PCR detection of as little as 0.5 pg of S. tritici genomic DNAwas possible. This is the first report on the detection of S. tritici DNA in naturally infested wheat seeds. This PCR based assay is simple, rapid, specific, sensitive and suitable for routine detection of the wheat pathogen in infested wheat seeds.
Centro de Investigaciones en Fitopatología - Materia
-
Ciencias Agrarias
Ciencias Naturales
Mycosphaerella graminicola
seedborne pathogen
Septoria leaf blotch
wheat - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by/4.0/
- Repositorio
.jpg)
- Institución
- Universidad Nacional de La Plata
- OAI Identificador
- oai:sedici.unlp.edu.ar:10915/136109
Ver los metadatos del registro completo
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A conventional PCR technique to detect <i>Septoria tritici</i> in wheat seedsConsolo, Verónica FabianaAlbani, Clara MaríaBerón, Corina M.Salerno, Graciela LidiaCordo, Cristina AliciaCiencias AgrariasCiencias NaturalesMycosphaerella graminicolaseedborne pathogenSeptoria leaf blotchwheatA PCR assay was developed for detection of wheat seed naturally contaminated with <i>Septoria tritici</i>. <i>S. tritici</i> specific primers were derived from strict alignment of ITS and α-tubulin sequences of the pathogen. The specificity of four sets of synthesised oligonucleotide pairs (A, B, C and D) were tested using isolates from S. tritici, other selected fungi and wheat seeds. A single DNA fragment was amplified from <i>S. tritici</i> isolates with all primer pairs, whereas no product was generated from other DNA sources. <i>S. tritici</i> was also detected in wheat seed lots collected from plants with variable pycnidial coverage on the upper two leaves. PCR detection of as little as 0.5 pg of <i>S. tritici</i> genomic DNAwas possible. This is the first report on the detection of <i>S. tritici</i> DNA in naturally infested wheat seeds. This PCR based assay is simple, rapid, specific, sensitive and suitable for routine detection of the wheat pathogen in infested wheat seeds.Centro de Investigaciones en Fitopatología2009-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf222-227http://sedici.unlp.edu.ar/handle/10915/136109enginfo:eu-repo/semantics/altIdentifier/issn/0815-3191info:eu-repo/semantics/altIdentifier/issn/1448-6032info:eu-repo/semantics/altIdentifier/issn/0310-1266info:eu-repo/semantics/altIdentifier/doi/10.1071/ap08099info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-17T10:14:41Zoai:sedici.unlp.edu.ar:10915/136109Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-17 10:14:41.936SEDICI (UNLP) - Universidad Nacional de La Platafalse |
| dc.title.none.fl_str_mv |
A conventional PCR technique to detect <i>Septoria tritici</i> in wheat seeds |
| title |
A conventional PCR technique to detect <i>Septoria tritici</i> in wheat seeds |
| spellingShingle |
A conventional PCR technique to detect <i>Septoria tritici</i> in wheat seeds Consolo, Verónica Fabiana Ciencias Agrarias Ciencias Naturales Mycosphaerella graminicola seedborne pathogen Septoria leaf blotch wheat |
| title_short |
A conventional PCR technique to detect <i>Septoria tritici</i> in wheat seeds |
| title_full |
A conventional PCR technique to detect <i>Septoria tritici</i> in wheat seeds |
| title_fullStr |
A conventional PCR technique to detect <i>Septoria tritici</i> in wheat seeds |
| title_full_unstemmed |
A conventional PCR technique to detect <i>Septoria tritici</i> in wheat seeds |
| title_sort |
A conventional PCR technique to detect <i>Septoria tritici</i> in wheat seeds |
| dc.creator.none.fl_str_mv |
Consolo, Verónica Fabiana Albani, Clara María Berón, Corina M. Salerno, Graciela Lidia Cordo, Cristina Alicia |
| author |
Consolo, Verónica Fabiana |
| author_facet |
Consolo, Verónica Fabiana Albani, Clara María Berón, Corina M. Salerno, Graciela Lidia Cordo, Cristina Alicia |
| author_role |
author |
| author2 |
Albani, Clara María Berón, Corina M. Salerno, Graciela Lidia Cordo, Cristina Alicia |
| author2_role |
author author author author |
| dc.subject.none.fl_str_mv |
Ciencias Agrarias Ciencias Naturales Mycosphaerella graminicola seedborne pathogen Septoria leaf blotch wheat |
| topic |
Ciencias Agrarias Ciencias Naturales Mycosphaerella graminicola seedborne pathogen Septoria leaf blotch wheat |
| dc.description.none.fl_txt_mv |
A PCR assay was developed for detection of wheat seed naturally contaminated with <i>Septoria tritici</i>. <i>S. tritici</i> specific primers were derived from strict alignment of ITS and α-tubulin sequences of the pathogen. The specificity of four sets of synthesised oligonucleotide pairs (A, B, C and D) were tested using isolates from S. tritici, other selected fungi and wheat seeds. A single DNA fragment was amplified from <i>S. tritici</i> isolates with all primer pairs, whereas no product was generated from other DNA sources. <i>S. tritici</i> was also detected in wheat seed lots collected from plants with variable pycnidial coverage on the upper two leaves. PCR detection of as little as 0.5 pg of <i>S. tritici</i> genomic DNAwas possible. This is the first report on the detection of <i>S. tritici</i> DNA in naturally infested wheat seeds. This PCR based assay is simple, rapid, specific, sensitive and suitable for routine detection of the wheat pathogen in infested wheat seeds. Centro de Investigaciones en Fitopatología |
| description |
A PCR assay was developed for detection of wheat seed naturally contaminated with <i>Septoria tritici</i>. <i>S. tritici</i> specific primers were derived from strict alignment of ITS and α-tubulin sequences of the pathogen. The specificity of four sets of synthesised oligonucleotide pairs (A, B, C and D) were tested using isolates from S. tritici, other selected fungi and wheat seeds. A single DNA fragment was amplified from <i>S. tritici</i> isolates with all primer pairs, whereas no product was generated from other DNA sources. <i>S. tritici</i> was also detected in wheat seed lots collected from plants with variable pycnidial coverage on the upper two leaves. PCR detection of as little as 0.5 pg of <i>S. tritici</i> genomic DNAwas possible. This is the first report on the detection of <i>S. tritici</i> DNA in naturally infested wheat seeds. This PCR based assay is simple, rapid, specific, sensitive and suitable for routine detection of the wheat pathogen in infested wheat seeds. |
| publishDate |
2009 |
| dc.date.none.fl_str_mv |
2009-05 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Articulo http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
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article |
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publishedVersion |
| dc.identifier.none.fl_str_mv |
http://sedici.unlp.edu.ar/handle/10915/136109 |
| url |
http://sedici.unlp.edu.ar/handle/10915/136109 |
| dc.language.none.fl_str_mv |
eng |
| language |
eng |
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openAccess |
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