A conventional PCR technique to detect Septoria tritici in wheat seeds

Autores
Consolo, Verónica Fabiana; Albani, Clara Maria; Berón, Corina Marta; Salerno, Graciela Lidia; Cordo, Cristina Alicia
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A PCR assay was developed for detection of wheat seed naturally contaminated with Septoria tritici. S. tritici specific primers were derived from strict alignment of ITS and -tubulin sequences of the pathogen. The specificity of four sets of synthesised oligonucleotide pairs (A, B, C and D) were tested using isolates from S. tritici, other selected fungi and wheat seeds. A single DNA fragment was amplified from S. tritici isolates with all primer pairs, whereas no product was generated from other DNA sources. S. tritici was also detected in wheat seed lots collected from plants with variable pycnidial coverage on the upper two leaves. PCR detection of as little as 0.5 pg of S. tritici genomic DNA was possible. This is the first report on the detection of S. tritici DNA in naturally infested wheat seeds. This PCR based assay is simple, rapid, specific, sensitive and suitable for routine detection of the wheat pathogen in infested wheat seeds.
Fil: Consolo, Verónica Fabiana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fundación para Investigaciones Biológicas Aplicadas. Centro de Estudios de Biodiversidad y Biotecnología; Argentina
Fil: Albani, Clara Maria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fundación para Investigaciones Biológicas Aplicadas. Centro de Estudios de Biodiversidad y Biotecnología; Argentina
Fil: Berón, Corina Marta. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fundación para Investigaciones Biológicas Aplicadas. Centro de Estudios de Biodiversidad y Biotecnología; Argentina
Fil: Salerno, Graciela Lidia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fundación para Investigaciones Biológicas Aplicadas. Centro de Estudios de Biodiversidad y Biotecnología; Argentina
Fil: Cordo, Cristina Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales. Departamento de Ciencias Biológicas. Centro de Investigaciones de Fitopatología. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigaciones de Fitopatología; Argentina
Materia
MYCOSPHAERELLA GRAMINICOLA
SEEDBORNE PATHOGEN
SEPTORIA LEAF BLOTCH
WHEAT
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/98124
Acceder
id CONICETDig_3e391a17085071d7aa7dd852987479c3
oai_identifier_str oai:ri.conicet.gov.ar:11336/98124
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling A conventional PCR technique to detect Septoria tritici in wheat seedsConsolo, Verónica FabianaAlbani, Clara MariaBerón, Corina MartaSalerno, Graciela LidiaCordo, Cristina AliciaMYCOSPHAERELLA GRAMINICOLASEEDBORNE PATHOGENSEPTORIA LEAF BLOTCHWHEAThttps://purl.org/becyt/ford/4.1https://purl.org/becyt/ford/4https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1A PCR assay was developed for detection of wheat seed naturally contaminated with Septoria tritici. S. tritici specific primers were derived from strict alignment of ITS and -tubulin sequences of the pathogen. The specificity of four sets of synthesised oligonucleotide pairs (A, B, C and D) were tested using isolates from S. tritici, other selected fungi and wheat seeds. A single DNA fragment was amplified from S. tritici isolates with all primer pairs, whereas no product was generated from other DNA sources. S. tritici was also detected in wheat seed lots collected from plants with variable pycnidial coverage on the upper two leaves. PCR detection of as little as 0.5 pg of S. tritici genomic DNA was possible. This is the first report on the detection of S. tritici DNA in naturally infested wheat seeds. This PCR based assay is simple, rapid, specific, sensitive and suitable for routine detection of the wheat pathogen in infested wheat seeds.Fil: Consolo, Verónica Fabiana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fundación para Investigaciones Biológicas Aplicadas. Centro de Estudios de Biodiversidad y Biotecnología; ArgentinaFil: Albani, Clara Maria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fundación para Investigaciones Biológicas Aplicadas. Centro de Estudios de Biodiversidad y Biotecnología; ArgentinaFil: Berón, Corina Marta. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fundación para Investigaciones Biológicas Aplicadas. Centro de Estudios de Biodiversidad y Biotecnología; ArgentinaFil: Salerno, Graciela Lidia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fundación para Investigaciones Biológicas Aplicadas. Centro de Estudios de Biodiversidad y Biotecnología; ArgentinaFil: Cordo, Cristina Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales. Departamento de Ciencias Biológicas. Centro de Investigaciones de Fitopatología. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigaciones de Fitopatología; ArgentinaCsiro Publishing2009-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/98124Consolo, Verónica Fabiana; Albani, Clara Maria; Berón, Corina Marta; Salerno, Graciela Lidia; Cordo, Cristina Alicia; A conventional PCR technique to detect Septoria tritici in wheat seeds; Csiro Publishing; Australasian Plant Pathology; 38; 3; 12-2009; 222-2270815-3191CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1071/AP08099info:eu-repo/semantics/altIdentifier/doi/10.1071/AP08099info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:10:37Zoai:ri.conicet.gov.ar:11336/98124instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:10:37.933CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv A conventional PCR technique to detect Septoria tritici in wheat seeds
title A conventional PCR technique to detect Septoria tritici in wheat seeds
spellingShingle A conventional PCR technique to detect Septoria tritici in wheat seeds
Consolo, Verónica Fabiana
MYCOSPHAERELLA GRAMINICOLA
SEEDBORNE PATHOGEN
SEPTORIA LEAF BLOTCH
WHEAT
title_short A conventional PCR technique to detect Septoria tritici in wheat seeds
title_full A conventional PCR technique to detect Septoria tritici in wheat seeds
title_fullStr A conventional PCR technique to detect Septoria tritici in wheat seeds
title_full_unstemmed A conventional PCR technique to detect Septoria tritici in wheat seeds
title_sort A conventional PCR technique to detect Septoria tritici in wheat seeds
dc.creator.none.fl_str_mv Consolo, Verónica Fabiana
Albani, Clara Maria
Berón, Corina Marta
Salerno, Graciela Lidia
Cordo, Cristina Alicia
author Consolo, Verónica Fabiana
author_facet Consolo, Verónica Fabiana
Albani, Clara Maria
Berón, Corina Marta
Salerno, Graciela Lidia
Cordo, Cristina Alicia
author_role author
author2 Albani, Clara Maria
Berón, Corina Marta
Salerno, Graciela Lidia
Cordo, Cristina Alicia
author2_role author
author
author
author
dc.subject.none.fl_str_mv MYCOSPHAERELLA GRAMINICOLA
SEEDBORNE PATHOGEN
SEPTORIA LEAF BLOTCH
WHEAT
topic MYCOSPHAERELLA GRAMINICOLA
SEEDBORNE PATHOGEN
SEPTORIA LEAF BLOTCH
WHEAT
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.1
https://purl.org/becyt/ford/4
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv A PCR assay was developed for detection of wheat seed naturally contaminated with Septoria tritici. S. tritici specific primers were derived from strict alignment of ITS and -tubulin sequences of the pathogen. The specificity of four sets of synthesised oligonucleotide pairs (A, B, C and D) were tested using isolates from S. tritici, other selected fungi and wheat seeds. A single DNA fragment was amplified from S. tritici isolates with all primer pairs, whereas no product was generated from other DNA sources. S. tritici was also detected in wheat seed lots collected from plants with variable pycnidial coverage on the upper two leaves. PCR detection of as little as 0.5 pg of S. tritici genomic DNA was possible. This is the first report on the detection of S. tritici DNA in naturally infested wheat seeds. This PCR based assay is simple, rapid, specific, sensitive and suitable for routine detection of the wheat pathogen in infested wheat seeds.
Fil: Consolo, Verónica Fabiana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fundación para Investigaciones Biológicas Aplicadas. Centro de Estudios de Biodiversidad y Biotecnología; Argentina
Fil: Albani, Clara Maria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fundación para Investigaciones Biológicas Aplicadas. Centro de Estudios de Biodiversidad y Biotecnología; Argentina
Fil: Berón, Corina Marta. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fundación para Investigaciones Biológicas Aplicadas. Centro de Estudios de Biodiversidad y Biotecnología; Argentina
Fil: Salerno, Graciela Lidia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fundación para Investigaciones Biológicas Aplicadas. Centro de Estudios de Biodiversidad y Biotecnología; Argentina
Fil: Cordo, Cristina Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales. Departamento de Ciencias Biológicas. Centro de Investigaciones de Fitopatología. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigaciones de Fitopatología; Argentina
description A PCR assay was developed for detection of wheat seed naturally contaminated with Septoria tritici. S. tritici specific primers were derived from strict alignment of ITS and -tubulin sequences of the pathogen. The specificity of four sets of synthesised oligonucleotide pairs (A, B, C and D) were tested using isolates from S. tritici, other selected fungi and wheat seeds. A single DNA fragment was amplified from S. tritici isolates with all primer pairs, whereas no product was generated from other DNA sources. S. tritici was also detected in wheat seed lots collected from plants with variable pycnidial coverage on the upper two leaves. PCR detection of as little as 0.5 pg of S. tritici genomic DNA was possible. This is the first report on the detection of S. tritici DNA in naturally infested wheat seeds. This PCR based assay is simple, rapid, specific, sensitive and suitable for routine detection of the wheat pathogen in infested wheat seeds.
publishDate 2009
dc.date.none.fl_str_mv 2009-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/98124
Consolo, Verónica Fabiana; Albani, Clara Maria; Berón, Corina Marta; Salerno, Graciela Lidia; Cordo, Cristina Alicia; A conventional PCR technique to detect Septoria tritici in wheat seeds; Csiro Publishing; Australasian Plant Pathology; 38; 3; 12-2009; 222-227
0815-3191
CONICET Digital
CONICET
url http://hdl.handle.net/11336/98124
identifier_str_mv Consolo, Verónica Fabiana; Albani, Clara Maria; Berón, Corina Marta; Salerno, Graciela Lidia; Cordo, Cristina Alicia; A conventional PCR technique to detect Septoria tritici in wheat seeds; Csiro Publishing; Australasian Plant Pathology; 38; 3; 12-2009; 222-227
0815-3191
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1071/AP08099
info:eu-repo/semantics/altIdentifier/doi/10.1071/AP08099
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Csiro Publishing
publisher.none.fl_str_mv Csiro Publishing
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846083254421028864
score 13.22299

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