A conventional PCR technique to detect Septoria tritici in wheat seeds
- Autores
- Consolo, Verónica Fabiana; Albani, C.M.; Berón, C.M.; Salerno, Graciela Lidia; Cordo, Cristina Alicia
- Año de publicación
- 2009
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión enviada
- Descripción
- A PCR assay was developed for detection of wheat seed naturally contaminated with Septoria tritici. S. tritici specific primers were derived from strict alignment of ITS and a-tubulin sequences of the pathogen. The specificity of four sets of synthesised oligonucleotide pairs (A, B, C and D) were tested using isolates from S. tritici, other selected fungi and wheat seeds. A single DNA fragment was amplified from S. tritici isolates with all primer pairs, whereas no product was generated from otherDNAsources. S. tritici was also detected in wheat seed lots collected from plants with variable pycnidial coverage on the upper two leaves. PCR detection of as little as 0.5 pg of S. tritici genomic DNAwas possible. This is the first report on the detection of S. tritici DNA in naturally infested wheat seeds. This PCR based assay is simple, rapid, specific, sensitive and suitable for routine detection of the wheat pathogen in infested wheat seeds.
- Materia
-
Agronomía, reproducción y protección de plantas
Mycosphaerella graminicola
seedborne pathogen
Septoria leaf blotch
wheat - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by-nc-nd/4.0/
- Repositorio
- Institución
- Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
- OAI Identificador
- oai:digital.cic.gba.gob.ar:11746/6993
Ver los metadatos del registro completo
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A conventional PCR technique to detect Septoria tritici in wheat seedsConsolo, Verónica FabianaAlbani, C.M.Berón, C.M.Salerno, Graciela LidiaCordo, Cristina AliciaAgronomía, reproducción y protección de plantasMycosphaerella graminicolaseedborne pathogenSeptoria leaf blotchwheatA PCR assay was developed for detection of wheat seed naturally contaminated with <em>Septoria tritici. S. tritici </em>specific primers were derived from strict alignment of ITS and a-tubulin sequences of the pathogen. The specificity of four sets of synthesised oligonucleotide pairs (A, B, C and D) were tested using isolates from <em>S. tritici</em>, other selected fungi and wheat seeds. A single DNA fragment was amplified from <em>S. tritici i</em>solates with all primer pairs, whereas no product was generated from otherDNAsources.<em> S. tritici </em>was also detected in wheat seed lots collected from plants with variable pycnidial coverage on the upper two leaves. PCR detection of as little as 0.5 pg of <em>S. tritici</em> genomic DNAwas possible. This is the first report on the detection of<em> S. tritici </em>DNA in naturally infested wheat seeds. This PCR based assay is simple, rapid, specific, sensitive and suitable for routine detection of the wheat pathogen in infested wheat seeds.2009-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/submittedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://digital.cic.gba.gob.ar/handle/11746/6993enginfo:eu-repo/semantics/altIdentifier/doi/10.1071/AP08099info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/4.0/reponame:CIC Digital (CICBA)instname:Comisión de Investigaciones Científicas de la Provincia de Buenos Airesinstacron:CICBA2025-10-16T09:27:37Zoai:digital.cic.gba.gob.ar:11746/6993Institucionalhttp://digital.cic.gba.gob.arOrganismo científico-tecnológicoNo correspondehttp://digital.cic.gba.gob.ar/oai/snrdmarisa.degiusti@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:94412025-10-16 09:27:37.316CIC Digital (CICBA) - Comisión de Investigaciones Científicas de la Provincia de Buenos Airesfalse |
dc.title.none.fl_str_mv |
A conventional PCR technique to detect Septoria tritici in wheat seeds |
title |
A conventional PCR technique to detect Septoria tritici in wheat seeds |
spellingShingle |
A conventional PCR technique to detect Septoria tritici in wheat seeds Consolo, Verónica Fabiana Agronomía, reproducción y protección de plantas Mycosphaerella graminicola seedborne pathogen Septoria leaf blotch wheat |
title_short |
A conventional PCR technique to detect Septoria tritici in wheat seeds |
title_full |
A conventional PCR technique to detect Septoria tritici in wheat seeds |
title_fullStr |
A conventional PCR technique to detect Septoria tritici in wheat seeds |
title_full_unstemmed |
A conventional PCR technique to detect Septoria tritici in wheat seeds |
title_sort |
A conventional PCR technique to detect Septoria tritici in wheat seeds |
dc.creator.none.fl_str_mv |
Consolo, Verónica Fabiana Albani, C.M. Berón, C.M. Salerno, Graciela Lidia Cordo, Cristina Alicia |
author |
Consolo, Verónica Fabiana |
author_facet |
Consolo, Verónica Fabiana Albani, C.M. Berón, C.M. Salerno, Graciela Lidia Cordo, Cristina Alicia |
author_role |
author |
author2 |
Albani, C.M. Berón, C.M. Salerno, Graciela Lidia Cordo, Cristina Alicia |
author2_role |
author author author author |
dc.subject.none.fl_str_mv |
Agronomía, reproducción y protección de plantas Mycosphaerella graminicola seedborne pathogen Septoria leaf blotch wheat |
topic |
Agronomía, reproducción y protección de plantas Mycosphaerella graminicola seedborne pathogen Septoria leaf blotch wheat |
dc.description.none.fl_txt_mv |
A PCR assay was developed for detection of wheat seed naturally contaminated with <em>Septoria tritici. S. tritici </em>specific primers were derived from strict alignment of ITS and a-tubulin sequences of the pathogen. The specificity of four sets of synthesised oligonucleotide pairs (A, B, C and D) were tested using isolates from <em>S. tritici</em>, other selected fungi and wheat seeds. A single DNA fragment was amplified from <em>S. tritici i</em>solates with all primer pairs, whereas no product was generated from otherDNAsources.<em> S. tritici </em>was also detected in wheat seed lots collected from plants with variable pycnidial coverage on the upper two leaves. PCR detection of as little as 0.5 pg of <em>S. tritici</em> genomic DNAwas possible. This is the first report on the detection of<em> S. tritici </em>DNA in naturally infested wheat seeds. This PCR based assay is simple, rapid, specific, sensitive and suitable for routine detection of the wheat pathogen in infested wheat seeds. |
description |
A PCR assay was developed for detection of wheat seed naturally contaminated with <em>Septoria tritici. S. tritici </em>specific primers were derived from strict alignment of ITS and a-tubulin sequences of the pathogen. The specificity of four sets of synthesised oligonucleotide pairs (A, B, C and D) were tested using isolates from <em>S. tritici</em>, other selected fungi and wheat seeds. A single DNA fragment was amplified from <em>S. tritici i</em>solates with all primer pairs, whereas no product was generated from otherDNAsources.<em> S. tritici </em>was also detected in wheat seed lots collected from plants with variable pycnidial coverage on the upper two leaves. PCR detection of as little as 0.5 pg of <em>S. tritici</em> genomic DNAwas possible. This is the first report on the detection of<em> S. tritici </em>DNA in naturally infested wheat seeds. This PCR based assay is simple, rapid, specific, sensitive and suitable for routine detection of the wheat pathogen in infested wheat seeds. |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009-05 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/submittedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
submittedVersion |
dc.identifier.none.fl_str_mv |
https://digital.cic.gba.gob.ar/handle/11746/6993 |
url |
https://digital.cic.gba.gob.ar/handle/11746/6993 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.1071/AP08099 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-nd/4.0/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by-nc-nd/4.0/ |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:CIC Digital (CICBA) instname:Comisión de Investigaciones Científicas de la Provincia de Buenos Aires instacron:CICBA |
reponame_str |
CIC Digital (CICBA) |
collection |
CIC Digital (CICBA) |
instname_str |
Comisión de Investigaciones Científicas de la Provincia de Buenos Aires |
instacron_str |
CICBA |
institution |
CICBA |
repository.name.fl_str_mv |
CIC Digital (CICBA) - Comisión de Investigaciones Científicas de la Provincia de Buenos Aires |
repository.mail.fl_str_mv |
marisa.degiusti@sedici.unlp.edu.ar |
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1846142637314146304 |
score |
13.22299 |