A conventional PCR technique to detect Septoria tritici in wheat seeds

Autores
Consolo, Verónica Fabiana; Albani, C.M.; Berón, C.M.; Salerno, Graciela Lidia; Cordo, Cristina Alicia
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión enviada
Descripción
A PCR assay was developed for detection of wheat seed naturally contaminated with Septoria tritici. S. tritici specific primers were derived from strict alignment of ITS and a-tubulin sequences of the pathogen. The specificity of four sets of synthesised oligonucleotide pairs (A, B, C and D) were tested using isolates from S. tritici, other selected fungi and wheat seeds. A single DNA fragment was amplified from S. tritici isolates with all primer pairs, whereas no product was generated from otherDNAsources. S. tritici was also detected in wheat seed lots collected from plants with variable pycnidial coverage on the upper two leaves. PCR detection of as little as 0.5 pg of S. tritici genomic DNAwas possible. This is the first report on the detection of S. tritici DNA in naturally infested wheat seeds. This PCR based assay is simple, rapid, specific, sensitive and suitable for routine detection of the wheat pathogen in infested wheat seeds.
Materia
Agronomía, reproducción y protección de plantas
Mycosphaerella graminicola
seedborne pathogen
Septoria leaf blotch
wheat
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-nd/4.0/
Repositorio
CIC Digital (CICBA)
Institución
Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
OAI Identificador
oai:digital.cic.gba.gob.ar:11746/6993

id CICBA_71e35ba8e93048993be05294d7c77ab9
oai_identifier_str oai:digital.cic.gba.gob.ar:11746/6993
network_acronym_str CICBA
repository_id_str 9441
network_name_str CIC Digital (CICBA)
spelling A conventional PCR technique to detect Septoria tritici in wheat seedsConsolo, Verónica FabianaAlbani, C.M.Berón, C.M.Salerno, Graciela LidiaCordo, Cristina AliciaAgronomía, reproducción y protección de plantasMycosphaerella graminicolaseedborne pathogenSeptoria leaf blotchwheatA PCR assay was developed for detection of wheat seed naturally contaminated with <em>Septoria tritici. S. tritici </em>specific primers were derived from strict alignment of ITS and a-tubulin sequences of the pathogen. The specificity of four sets of synthesised oligonucleotide pairs (A, B, C and D) were tested using isolates from <em>S. tritici</em>, other selected fungi and wheat seeds. A single DNA fragment was amplified from <em>S. tritici i</em>solates with all primer pairs, whereas no product was generated from otherDNAsources.<em> S. tritici </em>was also detected in wheat seed lots collected from plants with variable pycnidial coverage on the upper two leaves. PCR detection of as little as 0.5 pg of <em>S. tritici</em> genomic DNAwas possible. This is the first report on the detection of<em> S. tritici </em>DNA in naturally infested wheat seeds. This PCR based assay is simple, rapid, specific, sensitive and suitable for routine detection of the wheat pathogen in infested wheat seeds.2009-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/submittedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://digital.cic.gba.gob.ar/handle/11746/6993enginfo:eu-repo/semantics/altIdentifier/doi/10.1071/AP08099info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/4.0/reponame:CIC Digital (CICBA)instname:Comisión de Investigaciones Científicas de la Provincia de Buenos Airesinstacron:CICBA2025-10-16T09:27:37Zoai:digital.cic.gba.gob.ar:11746/6993Institucionalhttp://digital.cic.gba.gob.arOrganismo científico-tecnológicoNo correspondehttp://digital.cic.gba.gob.ar/oai/snrdmarisa.degiusti@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:94412025-10-16 09:27:37.316CIC Digital (CICBA) - Comisión de Investigaciones Científicas de la Provincia de Buenos Airesfalse
dc.title.none.fl_str_mv A conventional PCR technique to detect Septoria tritici in wheat seeds
title A conventional PCR technique to detect Septoria tritici in wheat seeds
spellingShingle A conventional PCR technique to detect Septoria tritici in wheat seeds
Consolo, Verónica Fabiana
Agronomía, reproducción y protección de plantas
Mycosphaerella graminicola
seedborne pathogen
Septoria leaf blotch
wheat
title_short A conventional PCR technique to detect Septoria tritici in wheat seeds
title_full A conventional PCR technique to detect Septoria tritici in wheat seeds
title_fullStr A conventional PCR technique to detect Septoria tritici in wheat seeds
title_full_unstemmed A conventional PCR technique to detect Septoria tritici in wheat seeds
title_sort A conventional PCR technique to detect Septoria tritici in wheat seeds
dc.creator.none.fl_str_mv Consolo, Verónica Fabiana
Albani, C.M.
Berón, C.M.
Salerno, Graciela Lidia
Cordo, Cristina Alicia
author Consolo, Verónica Fabiana
author_facet Consolo, Verónica Fabiana
Albani, C.M.
Berón, C.M.
Salerno, Graciela Lidia
Cordo, Cristina Alicia
author_role author
author2 Albani, C.M.
Berón, C.M.
Salerno, Graciela Lidia
Cordo, Cristina Alicia
author2_role author
author
author
author
dc.subject.none.fl_str_mv Agronomía, reproducción y protección de plantas
Mycosphaerella graminicola
seedborne pathogen
Septoria leaf blotch
wheat
topic Agronomía, reproducción y protección de plantas
Mycosphaerella graminicola
seedborne pathogen
Septoria leaf blotch
wheat
dc.description.none.fl_txt_mv A PCR assay was developed for detection of wheat seed naturally contaminated with <em>Septoria tritici. S. tritici </em>specific primers were derived from strict alignment of ITS and a-tubulin sequences of the pathogen. The specificity of four sets of synthesised oligonucleotide pairs (A, B, C and D) were tested using isolates from <em>S. tritici</em>, other selected fungi and wheat seeds. A single DNA fragment was amplified from <em>S. tritici i</em>solates with all primer pairs, whereas no product was generated from otherDNAsources.<em> S. tritici </em>was also detected in wheat seed lots collected from plants with variable pycnidial coverage on the upper two leaves. PCR detection of as little as 0.5 pg of <em>S. tritici</em> genomic DNAwas possible. This is the first report on the detection of<em> S. tritici </em>DNA in naturally infested wheat seeds. This PCR based assay is simple, rapid, specific, sensitive and suitable for routine detection of the wheat pathogen in infested wheat seeds.
description A PCR assay was developed for detection of wheat seed naturally contaminated with <em>Septoria tritici. S. tritici </em>specific primers were derived from strict alignment of ITS and a-tubulin sequences of the pathogen. The specificity of four sets of synthesised oligonucleotide pairs (A, B, C and D) were tested using isolates from <em>S. tritici</em>, other selected fungi and wheat seeds. A single DNA fragment was amplified from <em>S. tritici i</em>solates with all primer pairs, whereas no product was generated from otherDNAsources.<em> S. tritici </em>was also detected in wheat seed lots collected from plants with variable pycnidial coverage on the upper two leaves. PCR detection of as little as 0.5 pg of <em>S. tritici</em> genomic DNAwas possible. This is the first report on the detection of<em> S. tritici </em>DNA in naturally infested wheat seeds. This PCR based assay is simple, rapid, specific, sensitive and suitable for routine detection of the wheat pathogen in infested wheat seeds.
publishDate 2009
dc.date.none.fl_str_mv 2009-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/submittedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str submittedVersion
dc.identifier.none.fl_str_mv https://digital.cic.gba.gob.ar/handle/11746/6993
url https://digital.cic.gba.gob.ar/handle/11746/6993
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1071/AP08099
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:CIC Digital (CICBA)
instname:Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
instacron:CICBA
reponame_str CIC Digital (CICBA)
collection CIC Digital (CICBA)
instname_str Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
instacron_str CICBA
institution CICBA
repository.name.fl_str_mv CIC Digital (CICBA) - Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
repository.mail.fl_str_mv marisa.degiusti@sedici.unlp.edu.ar
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