Sexually Dimorphic Effects of Aging on Rat Somatotroph Cells: An Immunohistochemical and Ultrastructural Study

Autores
Jurado, Susana Beatriz; Console-Avegliano, Gloria Miriam; Gómez Dumm, César Leandro Alberto
Año de publicación
1998
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Aging produces alterations in certain functions of the hypothalamo-pituitary axis that result in sexually dimorphic changes in the somatotrophs. Since quantitative morphological data on these age-associated alterations are scarce, we prompted to make a morphometric immunohistochemical assessment as well as undertake an ultrastructural study of the somatotrophic (GH) cell population in male and female rats of different ages. Young (3-month-old), old (20-month-old), and senescent (29-month-old) Sprague-Dawley rats of both sexes were sacrificed by rapid decapitation, their pituitaries immediately dissected out, and processed for both immunohistochemistry and electron microscopy. Analysis of different morphometric parameters revealed that surface density, volume density, and cell density significantly decreased in old and senescent rats as compared to young animals, with this reduction being clearly more marked in females. Both the GH-cell area and perimeter decreased in senescent male rats, while these parameters increased in senescent females. The ultrastructure of the GH cells from old and senescent animals of both sexes evinced changes suggestive of an immature state, with some somatotrophs having the appearance of cells undergoing an involutive process. We conclude that aging has a differential impact on the GH cells of male and female rats with respect to the immunohistochemical and ultrastructural features of that cell population.
Facultad de Ciencias Veterinarias
Facultad de Ciencias Médicas
Materia
Ciencias Médicas
Aging
Pituitary gland
Sexual dimorphism
Quantitative immunohistochemistry
Somatotroph
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/134977
Acceder
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network_name_str SEDICI (UNLP)
spelling Sexually Dimorphic Effects of Aging on Rat Somatotroph Cells: An Immunohistochemical and Ultrastructural StudyJurado, Susana BeatrizConsole-Avegliano, Gloria MiriamGómez Dumm, César Leandro AlbertoCiencias MédicasAgingPituitary glandSexual dimorphismQuantitative immunohistochemistrySomatotrophAging produces alterations in certain functions of the hypothalamo-pituitary axis that result in sexually dimorphic changes in the somatotrophs. Since quantitative morphological data on these age-associated alterations are scarce, we prompted to make a morphometric immunohistochemical assessment as well as undertake an ultrastructural study of the somatotrophic (GH) cell population in male and female rats of different ages. Young (3-month-old), old (20-month-old), and senescent (29-month-old) Sprague-Dawley rats of both sexes were sacrificed by rapid decapitation, their pituitaries immediately dissected out, and processed for both immunohistochemistry and electron microscopy. Analysis of different morphometric parameters revealed that surface density, volume density, and cell density significantly decreased in old and senescent rats as compared to young animals, with this reduction being clearly more marked in females. Both the GH-cell area and perimeter decreased in senescent male rats, while these parameters increased in senescent females. The ultrastructure of the GH cells from old and senescent animals of both sexes evinced changes suggestive of an immature state, with some somatotrophs having the appearance of cells undergoing an involutive process. We conclude that aging has a differential impact on the GH cells of male and female rats with respect to the immunohistochemical and ultrastructural features of that cell population.Facultad de Ciencias VeterinariasFacultad de Ciencias Médicas1998info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf705-711http://sedici.unlp.edu.ar/handle/10915/134977enginfo:eu-repo/semantics/altIdentifier/issn/0916-7250info:eu-repo/semantics/altIdentifier/issn/1347-7439info:eu-repo/semantics/altIdentifier/doi/10.1292/jvms.60.705info:eu-repo/semantics/altIdentifier/pmid/9673941info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-03T11:04:19Zoai:sedici.unlp.edu.ar:10915/134977Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-03 11:04:19.474SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Sexually Dimorphic Effects of Aging on Rat Somatotroph Cells: An Immunohistochemical and Ultrastructural Study
title Sexually Dimorphic Effects of Aging on Rat Somatotroph Cells: An Immunohistochemical and Ultrastructural Study
spellingShingle Sexually Dimorphic Effects of Aging on Rat Somatotroph Cells: An Immunohistochemical and Ultrastructural Study
Jurado, Susana Beatriz
Ciencias Médicas
Aging
Pituitary gland
Sexual dimorphism
Quantitative immunohistochemistry
Somatotroph
title_short Sexually Dimorphic Effects of Aging on Rat Somatotroph Cells: An Immunohistochemical and Ultrastructural Study
title_full Sexually Dimorphic Effects of Aging on Rat Somatotroph Cells: An Immunohistochemical and Ultrastructural Study
title_fullStr Sexually Dimorphic Effects of Aging on Rat Somatotroph Cells: An Immunohistochemical and Ultrastructural Study
title_full_unstemmed Sexually Dimorphic Effects of Aging on Rat Somatotroph Cells: An Immunohistochemical and Ultrastructural Study
title_sort Sexually Dimorphic Effects of Aging on Rat Somatotroph Cells: An Immunohistochemical and Ultrastructural Study
dc.creator.none.fl_str_mv Jurado, Susana Beatriz
Console-Avegliano, Gloria Miriam
Gómez Dumm, César Leandro Alberto
author Jurado, Susana Beatriz
author_facet Jurado, Susana Beatriz
Console-Avegliano, Gloria Miriam
Gómez Dumm, César Leandro Alberto
author_role author
author2 Console-Avegliano, Gloria Miriam
Gómez Dumm, César Leandro Alberto
author2_role author
author
dc.subject.none.fl_str_mv Ciencias Médicas
Aging
Pituitary gland
Sexual dimorphism
Quantitative immunohistochemistry
Somatotroph
topic Ciencias Médicas
Aging
Pituitary gland
Sexual dimorphism
Quantitative immunohistochemistry
Somatotroph
dc.description.none.fl_txt_mv Aging produces alterations in certain functions of the hypothalamo-pituitary axis that result in sexually dimorphic changes in the somatotrophs. Since quantitative morphological data on these age-associated alterations are scarce, we prompted to make a morphometric immunohistochemical assessment as well as undertake an ultrastructural study of the somatotrophic (GH) cell population in male and female rats of different ages. Young (3-month-old), old (20-month-old), and senescent (29-month-old) Sprague-Dawley rats of both sexes were sacrificed by rapid decapitation, their pituitaries immediately dissected out, and processed for both immunohistochemistry and electron microscopy. Analysis of different morphometric parameters revealed that surface density, volume density, and cell density significantly decreased in old and senescent rats as compared to young animals, with this reduction being clearly more marked in females. Both the GH-cell area and perimeter decreased in senescent male rats, while these parameters increased in senescent females. The ultrastructure of the GH cells from old and senescent animals of both sexes evinced changes suggestive of an immature state, with some somatotrophs having the appearance of cells undergoing an involutive process. We conclude that aging has a differential impact on the GH cells of male and female rats with respect to the immunohistochemical and ultrastructural features of that cell population.
Facultad de Ciencias Veterinarias
Facultad de Ciencias Médicas
description Aging produces alterations in certain functions of the hypothalamo-pituitary axis that result in sexually dimorphic changes in the somatotrophs. Since quantitative morphological data on these age-associated alterations are scarce, we prompted to make a morphometric immunohistochemical assessment as well as undertake an ultrastructural study of the somatotrophic (GH) cell population in male and female rats of different ages. Young (3-month-old), old (20-month-old), and senescent (29-month-old) Sprague-Dawley rats of both sexes were sacrificed by rapid decapitation, their pituitaries immediately dissected out, and processed for both immunohistochemistry and electron microscopy. Analysis of different morphometric parameters revealed that surface density, volume density, and cell density significantly decreased in old and senescent rats as compared to young animals, with this reduction being clearly more marked in females. Both the GH-cell area and perimeter decreased in senescent male rats, while these parameters increased in senescent females. The ultrastructure of the GH cells from old and senescent animals of both sexes evinced changes suggestive of an immature state, with some somatotrophs having the appearance of cells undergoing an involutive process. We conclude that aging has a differential impact on the GH cells of male and female rats with respect to the immunohistochemical and ultrastructural features of that cell population.
publishDate 1998
dc.date.none.fl_str_mv 1998
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/134977
url http://sedici.unlp.edu.ar/handle/10915/134977
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/0916-7250
info:eu-repo/semantics/altIdentifier/issn/1347-7439
info:eu-repo/semantics/altIdentifier/doi/10.1292/jvms.60.705
info:eu-repo/semantics/altIdentifier/pmid/9673941
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
705-711
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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score 13.13397

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