Morphometric and ultrastructural analysis of different pituitary cell populations in undernourished monkeys

Autores
Console de Avegliano, Gloria Miriam; Jurado, Susana Beatriz; Oyhenart, Evelia Edith; Ferese, C.; Pucciarelli, Hector Mario; Gomez Dumm, Cesar Leandro Alberto
Año de publicación
2001
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Undernutrition elicited by a low-protein diet determines a marked reduction of hypophyseal activity and affects the function of the respective target organs. The objective of the present investigation was to study the ultrastructural and quantitative immunohistochemical changes of the different pituitary cell populations in undernourished monkeys that had been previously shown to have significant changes in craniofacial growth. Twenty Saimiri sciureus boliviensis monkeys of both sexes were used. The animals were born in captivity and were separated into two groups at one year of age, i.e., control and undernourished animals. The monkeys were fed ad libitum a 20% (control group) and a 10% (experimental group) protein diet for two years. Pituitaries were processed for light and electron microscopy. The former was immunolubeled with anti-GH, -PRL, -LH, -FSH, -ACTH, and -TSH sera. Volume density and cell density were measured using an image analyzer. Quantitative immunohistochemistry revealed a decrease in these parameters with regard to somatotrophs, lactotrophs, gonadotrophs and thyrotrophs from undernourished animals compared to control ones. In these populations, the ultrastructural study showed changes suggesting compensatory hyperfunction. On the contrary, no significant changes were found in the morphometric parameters or the ultrastructure of the corticotroph population. We conclude that in undernourished monkeys the somatotroph, lactotroph, gonadotroph, and thyrotroph cell populations showed quantitative immunohistochemical changes that can be correlated with ultrastructural findings.
Fil: Console de Avegliano, Gloria Miriam. Universidad Nacional de La Plata. Facultad de Ciencias Médicas; Argentina
Fil: Jurado, Susana Beatriz. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Fil: Oyhenart, Evelia Edith. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Centro de Investigaciones en Genética Básica y Aplicada; Argentina
Fil: Ferese, C.. Universidad Nacional de La Plata. Facultad de Ciencias Médicas; Argentina
Fil: Pucciarelli, Hector Mario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Centro de Investigaciones en Genética Básica y Aplicada; Argentina
Fil: Gomez Dumm, Cesar Leandro Alberto. Universidad Nacional de La Plata. Facultad de Ciencias Médicas; Argentina
Materia
PITUITARY GLAND
QUANTITATIVE IMMUNOHISTOCHEMISTRY
SQUIRREL MONKEYS
ULTRASTRUCTURE
UNDERNUTRITION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/84626
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/84626
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Morphometric and ultrastructural analysis of different pituitary cell populations in undernourished monkeysConsole de Avegliano, Gloria MiriamJurado, Susana BeatrizOyhenart, Evelia EdithFerese, C.Pucciarelli, Hector MarioGomez Dumm, Cesar Leandro AlbertoPITUITARY GLANDQUANTITATIVE IMMUNOHISTOCHEMISTRYSQUIRREL MONKEYSULTRASTRUCTUREUNDERNUTRITIONhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Undernutrition elicited by a low-protein diet determines a marked reduction of hypophyseal activity and affects the function of the respective target organs. The objective of the present investigation was to study the ultrastructural and quantitative immunohistochemical changes of the different pituitary cell populations in undernourished monkeys that had been previously shown to have significant changes in craniofacial growth. Twenty Saimiri sciureus boliviensis monkeys of both sexes were used. The animals were born in captivity and were separated into two groups at one year of age, i.e., control and undernourished animals. The monkeys were fed ad libitum a 20% (control group) and a 10% (experimental group) protein diet for two years. Pituitaries were processed for light and electron microscopy. The former was immunolubeled with anti-GH, -PRL, -LH, -FSH, -ACTH, and -TSH sera. Volume density and cell density were measured using an image analyzer. Quantitative immunohistochemistry revealed a decrease in these parameters with regard to somatotrophs, lactotrophs, gonadotrophs and thyrotrophs from undernourished animals compared to control ones. In these populations, the ultrastructural study showed changes suggesting compensatory hyperfunction. On the contrary, no significant changes were found in the morphometric parameters or the ultrastructure of the corticotroph population. We conclude that in undernourished monkeys the somatotroph, lactotroph, gonadotroph, and thyrotroph cell populations showed quantitative immunohistochemical changes that can be correlated with ultrastructural findings.Fil: Console de Avegliano, Gloria Miriam. Universidad Nacional de La Plata. Facultad de Ciencias Médicas; ArgentinaFil: Jurado, Susana Beatriz. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; ArgentinaFil: Oyhenart, Evelia Edith. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Centro de Investigaciones en Genética Básica y Aplicada; ArgentinaFil: Ferese, C.. Universidad Nacional de La Plata. Facultad de Ciencias Médicas; ArgentinaFil: Pucciarelli, Hector Mario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Centro de Investigaciones en Genética Básica y Aplicada; ArgentinaFil: Gomez Dumm, Cesar Leandro Alberto. Universidad Nacional de La Plata. Facultad de Ciencias Médicas; ArgentinaAssociação Brasileira de Divulgação Científica2001-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/84626Console de Avegliano, Gloria Miriam; Jurado, Susana Beatriz; Oyhenart, Evelia Edith; Ferese, C.; Pucciarelli, Hector Mario; et al.; Morphometric and ultrastructural analysis of different pituitary cell populations in undernourished monkeys; Associação Brasileira de Divulgação Científica; Brazilian Journal of Medical and Biological Research; 34; 1; 12-2001; 65-740100-879X1414-431XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://ref.scielo.org/qjy4njinfo:eu-repo/semantics/altIdentifier/doi/10.1590/S0100-879X2001000100008info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:07:38Zoai:ri.conicet.gov.ar:11336/84626instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:07:38.731CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Morphometric and ultrastructural analysis of different pituitary cell populations in undernourished monkeys
title Morphometric and ultrastructural analysis of different pituitary cell populations in undernourished monkeys
spellingShingle Morphometric and ultrastructural analysis of different pituitary cell populations in undernourished monkeys
Console de Avegliano, Gloria Miriam
PITUITARY GLAND
QUANTITATIVE IMMUNOHISTOCHEMISTRY
SQUIRREL MONKEYS
ULTRASTRUCTURE
UNDERNUTRITION
title_short Morphometric and ultrastructural analysis of different pituitary cell populations in undernourished monkeys
title_full Morphometric and ultrastructural analysis of different pituitary cell populations in undernourished monkeys
title_fullStr Morphometric and ultrastructural analysis of different pituitary cell populations in undernourished monkeys
title_full_unstemmed Morphometric and ultrastructural analysis of different pituitary cell populations in undernourished monkeys
title_sort Morphometric and ultrastructural analysis of different pituitary cell populations in undernourished monkeys
dc.creator.none.fl_str_mv Console de Avegliano, Gloria Miriam
Jurado, Susana Beatriz
Oyhenart, Evelia Edith
Ferese, C.
Pucciarelli, Hector Mario
Gomez Dumm, Cesar Leandro Alberto
author Console de Avegliano, Gloria Miriam
author_facet Console de Avegliano, Gloria Miriam
Jurado, Susana Beatriz
Oyhenart, Evelia Edith
Ferese, C.
Pucciarelli, Hector Mario
Gomez Dumm, Cesar Leandro Alberto
author_role author
author2 Jurado, Susana Beatriz
Oyhenart, Evelia Edith
Ferese, C.
Pucciarelli, Hector Mario
Gomez Dumm, Cesar Leandro Alberto
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv PITUITARY GLAND
QUANTITATIVE IMMUNOHISTOCHEMISTRY
SQUIRREL MONKEYS
ULTRASTRUCTURE
UNDERNUTRITION
topic PITUITARY GLAND
QUANTITATIVE IMMUNOHISTOCHEMISTRY
SQUIRREL MONKEYS
ULTRASTRUCTURE
UNDERNUTRITION
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Undernutrition elicited by a low-protein diet determines a marked reduction of hypophyseal activity and affects the function of the respective target organs. The objective of the present investigation was to study the ultrastructural and quantitative immunohistochemical changes of the different pituitary cell populations in undernourished monkeys that had been previously shown to have significant changes in craniofacial growth. Twenty Saimiri sciureus boliviensis monkeys of both sexes were used. The animals were born in captivity and were separated into two groups at one year of age, i.e., control and undernourished animals. The monkeys were fed ad libitum a 20% (control group) and a 10% (experimental group) protein diet for two years. Pituitaries were processed for light and electron microscopy. The former was immunolubeled with anti-GH, -PRL, -LH, -FSH, -ACTH, and -TSH sera. Volume density and cell density were measured using an image analyzer. Quantitative immunohistochemistry revealed a decrease in these parameters with regard to somatotrophs, lactotrophs, gonadotrophs and thyrotrophs from undernourished animals compared to control ones. In these populations, the ultrastructural study showed changes suggesting compensatory hyperfunction. On the contrary, no significant changes were found in the morphometric parameters or the ultrastructure of the corticotroph population. We conclude that in undernourished monkeys the somatotroph, lactotroph, gonadotroph, and thyrotroph cell populations showed quantitative immunohistochemical changes that can be correlated with ultrastructural findings.
Fil: Console de Avegliano, Gloria Miriam. Universidad Nacional de La Plata. Facultad de Ciencias Médicas; Argentina
Fil: Jurado, Susana Beatriz. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Fil: Oyhenart, Evelia Edith. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Centro de Investigaciones en Genética Básica y Aplicada; Argentina
Fil: Ferese, C.. Universidad Nacional de La Plata. Facultad de Ciencias Médicas; Argentina
Fil: Pucciarelli, Hector Mario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Centro de Investigaciones en Genética Básica y Aplicada; Argentina
Fil: Gomez Dumm, Cesar Leandro Alberto. Universidad Nacional de La Plata. Facultad de Ciencias Médicas; Argentina
description Undernutrition elicited by a low-protein diet determines a marked reduction of hypophyseal activity and affects the function of the respective target organs. The objective of the present investigation was to study the ultrastructural and quantitative immunohistochemical changes of the different pituitary cell populations in undernourished monkeys that had been previously shown to have significant changes in craniofacial growth. Twenty Saimiri sciureus boliviensis monkeys of both sexes were used. The animals were born in captivity and were separated into two groups at one year of age, i.e., control and undernourished animals. The monkeys were fed ad libitum a 20% (control group) and a 10% (experimental group) protein diet for two years. Pituitaries were processed for light and electron microscopy. The former was immunolubeled with anti-GH, -PRL, -LH, -FSH, -ACTH, and -TSH sera. Volume density and cell density were measured using an image analyzer. Quantitative immunohistochemistry revealed a decrease in these parameters with regard to somatotrophs, lactotrophs, gonadotrophs and thyrotrophs from undernourished animals compared to control ones. In these populations, the ultrastructural study showed changes suggesting compensatory hyperfunction. On the contrary, no significant changes were found in the morphometric parameters or the ultrastructure of the corticotroph population. We conclude that in undernourished monkeys the somatotroph, lactotroph, gonadotroph, and thyrotroph cell populations showed quantitative immunohistochemical changes that can be correlated with ultrastructural findings.
publishDate 2001
dc.date.none.fl_str_mv 2001-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/84626
Console de Avegliano, Gloria Miriam; Jurado, Susana Beatriz; Oyhenart, Evelia Edith; Ferese, C.; Pucciarelli, Hector Mario; et al.; Morphometric and ultrastructural analysis of different pituitary cell populations in undernourished monkeys; Associação Brasileira de Divulgação Científica; Brazilian Journal of Medical and Biological Research; 34; 1; 12-2001; 65-74
0100-879X
1414-431X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/84626
identifier_str_mv Console de Avegliano, Gloria Miriam; Jurado, Susana Beatriz; Oyhenart, Evelia Edith; Ferese, C.; Pucciarelli, Hector Mario; et al.; Morphometric and ultrastructural analysis of different pituitary cell populations in undernourished monkeys; Associação Brasileira de Divulgação Científica; Brazilian Journal of Medical and Biological Research; 34; 1; 12-2001; 65-74
0100-879X
1414-431X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://ref.scielo.org/qjy4nj
info:eu-repo/semantics/altIdentifier/doi/10.1590/S0100-879X2001000100008
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Associação Brasileira de Divulgação Científica
publisher.none.fl_str_mv Associação Brasileira de Divulgação Científica
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.993085

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