Magnetic field-assisted gene delivery: achievements and therapeutic potential

Autores
Schwerdt, José Ignacio; Goya, Gerardo F.; Calatayud, M. Pilar; Hereñú, Claudia Beatriz; Reggiani, Paula Cecilia; Goya, Rodolfo Gustavo
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The discovery in the early 2000’s that magnetic nanoparticles (MNPs) complexed to nonviral or viral vectors can, in the presence of an external magnetic field, greatly enhance gene transfer into cells has raised much interest. This technique, called magnetofection, was initially developed mainly to improve gene transfer in cell cultures, a simpler and more easily controllable scenario than in vivo models. These studies provided evidence for some unique capabilities of magnetofection. Progressively, the interest in magnetofection expanded to its application in animal models and led to the association of this technique with another technology, magnetic drug targeting (MDT). This combination offers the possibility to develop more efficient and less invasive gene therapy strategies for a number of major pathologies like cancer, neurodegeneration and myocardial infarction. The goal of MDT is to concentrate MNPs functionalized with therapeutic drugs, in target areas of the body by means of properly focused external magnetic fields. The availability of stable, nontoxic MNP-gene vector complexes now offers the opportunity to develop magnetic gene targeting (MGT), a variant of MDT in which the gene coding for a therapeutic molecule, rather than the molecule itself, is delivered to a therapeutic target area in the body. This article will first outline the principle of magnetofection, subsequently describing the properties of the magnetic fields and MNPs used in this technique. Next, it will review the results achieved by magnetofection in cell cultures. Last, the potential of MGT for implementing minimally invasive gene therapy will be discussed.
Instituto de Investigaciones Bioquímicas de La Plata
Materia
Bioquímica
Gene delivery
Magnetic nanoparticles
Magnetofection
Magnetic gene targeting
Minimal invasiveness- nanomedicine
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/127940

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spelling Magnetic field-assisted gene delivery: achievements and therapeutic potentialSchwerdt, José IgnacioGoya, Gerardo F.Calatayud, M. PilarHereñú, Claudia BeatrizReggiani, Paula CeciliaGoya, Rodolfo GustavoBioquímicaGene deliveryMagnetic nanoparticlesMagnetofectionMagnetic gene targetingMinimal invasiveness- nanomedicineThe discovery in the early 2000’s that magnetic nanoparticles (MNPs) complexed to nonviral or viral vectors can, in the presence of an external magnetic field, greatly enhance gene transfer into cells has raised much interest. This technique, called magnetofection, was initially developed mainly to improve gene transfer in cell cultures, a simpler and more easily controllable scenario than in vivo models. These studies provided evidence for some unique capabilities of magnetofection. Progressively, the interest in magnetofection expanded to its application in animal models and led to the association of this technique with another technology, magnetic drug targeting (MDT). This combination offers the possibility to develop more efficient and less invasive gene therapy strategies for a number of major pathologies like cancer, neurodegeneration and myocardial infarction. The goal of MDT is to concentrate MNPs functionalized with therapeutic drugs, in target areas of the body by means of properly focused external magnetic fields. The availability of stable, nontoxic MNP-gene vector complexes now offers the opportunity to develop magnetic gene targeting (MGT), a variant of MDT in which the gene coding for a therapeutic molecule, rather than the molecule itself, is delivered to a therapeutic target area in the body. This article will first outline the principle of magnetofection, subsequently describing the properties of the magnetic fields and MNPs used in this technique. Next, it will review the results achieved by magnetofection in cell cultures. Last, the potential of MGT for implementing minimally invasive gene therapy will be discussed.Instituto de Investigaciones Bioquímicas de La Plata2012-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf116-126http://sedici.unlp.edu.ar/handle/10915/127940enginfo:eu-repo/semantics/altIdentifier/issn/1875-5631info:eu-repo/semantics/altIdentifier/issn/1566-5232info:eu-repo/semantics/altIdentifier/pmid/22348552info:eu-repo/semantics/altIdentifier/doi/10.2174/156652312800099616info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:30:58Zoai:sedici.unlp.edu.ar:10915/127940Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:30:59.12SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Magnetic field-assisted gene delivery: achievements and therapeutic potential
title Magnetic field-assisted gene delivery: achievements and therapeutic potential
spellingShingle Magnetic field-assisted gene delivery: achievements and therapeutic potential
Schwerdt, José Ignacio
Bioquímica
Gene delivery
Magnetic nanoparticles
Magnetofection
Magnetic gene targeting
Minimal invasiveness- nanomedicine
title_short Magnetic field-assisted gene delivery: achievements and therapeutic potential
title_full Magnetic field-assisted gene delivery: achievements and therapeutic potential
title_fullStr Magnetic field-assisted gene delivery: achievements and therapeutic potential
title_full_unstemmed Magnetic field-assisted gene delivery: achievements and therapeutic potential
title_sort Magnetic field-assisted gene delivery: achievements and therapeutic potential
dc.creator.none.fl_str_mv Schwerdt, José Ignacio
Goya, Gerardo F.
Calatayud, M. Pilar
Hereñú, Claudia Beatriz
Reggiani, Paula Cecilia
Goya, Rodolfo Gustavo
author Schwerdt, José Ignacio
author_facet Schwerdt, José Ignacio
Goya, Gerardo F.
Calatayud, M. Pilar
Hereñú, Claudia Beatriz
Reggiani, Paula Cecilia
Goya, Rodolfo Gustavo
author_role author
author2 Goya, Gerardo F.
Calatayud, M. Pilar
Hereñú, Claudia Beatriz
Reggiani, Paula Cecilia
Goya, Rodolfo Gustavo
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Bioquímica
Gene delivery
Magnetic nanoparticles
Magnetofection
Magnetic gene targeting
Minimal invasiveness- nanomedicine
topic Bioquímica
Gene delivery
Magnetic nanoparticles
Magnetofection
Magnetic gene targeting
Minimal invasiveness- nanomedicine
dc.description.none.fl_txt_mv The discovery in the early 2000’s that magnetic nanoparticles (MNPs) complexed to nonviral or viral vectors can, in the presence of an external magnetic field, greatly enhance gene transfer into cells has raised much interest. This technique, called magnetofection, was initially developed mainly to improve gene transfer in cell cultures, a simpler and more easily controllable scenario than in vivo models. These studies provided evidence for some unique capabilities of magnetofection. Progressively, the interest in magnetofection expanded to its application in animal models and led to the association of this technique with another technology, magnetic drug targeting (MDT). This combination offers the possibility to develop more efficient and less invasive gene therapy strategies for a number of major pathologies like cancer, neurodegeneration and myocardial infarction. The goal of MDT is to concentrate MNPs functionalized with therapeutic drugs, in target areas of the body by means of properly focused external magnetic fields. The availability of stable, nontoxic MNP-gene vector complexes now offers the opportunity to develop magnetic gene targeting (MGT), a variant of MDT in which the gene coding for a therapeutic molecule, rather than the molecule itself, is delivered to a therapeutic target area in the body. This article will first outline the principle of magnetofection, subsequently describing the properties of the magnetic fields and MNPs used in this technique. Next, it will review the results achieved by magnetofection in cell cultures. Last, the potential of MGT for implementing minimally invasive gene therapy will be discussed.
Instituto de Investigaciones Bioquímicas de La Plata
description The discovery in the early 2000’s that magnetic nanoparticles (MNPs) complexed to nonviral or viral vectors can, in the presence of an external magnetic field, greatly enhance gene transfer into cells has raised much interest. This technique, called magnetofection, was initially developed mainly to improve gene transfer in cell cultures, a simpler and more easily controllable scenario than in vivo models. These studies provided evidence for some unique capabilities of magnetofection. Progressively, the interest in magnetofection expanded to its application in animal models and led to the association of this technique with another technology, magnetic drug targeting (MDT). This combination offers the possibility to develop more efficient and less invasive gene therapy strategies for a number of major pathologies like cancer, neurodegeneration and myocardial infarction. The goal of MDT is to concentrate MNPs functionalized with therapeutic drugs, in target areas of the body by means of properly focused external magnetic fields. The availability of stable, nontoxic MNP-gene vector complexes now offers the opportunity to develop magnetic gene targeting (MGT), a variant of MDT in which the gene coding for a therapeutic molecule, rather than the molecule itself, is delivered to a therapeutic target area in the body. This article will first outline the principle of magnetofection, subsequently describing the properties of the magnetic fields and MNPs used in this technique. Next, it will review the results achieved by magnetofection in cell cultures. Last, the potential of MGT for implementing minimally invasive gene therapy will be discussed.
publishDate 2012
dc.date.none.fl_str_mv 2012-04-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
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format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/127940
url http://sedici.unlp.edu.ar/handle/10915/127940
dc.language.none.fl_str_mv eng
language eng
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info:eu-repo/semantics/altIdentifier/issn/1566-5232
info:eu-repo/semantics/altIdentifier/pmid/22348552
info:eu-repo/semantics/altIdentifier/doi/10.2174/156652312800099616
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
116-126
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instname_str Universidad Nacional de La Plata
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repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
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