Effects of physiochemical factors on prokaryotic Biodiversity in Malaysian circumneutral hot springs

Autores
Chan, Chia S.; Chan, Kok-Gan; Ee, Robson; Hong, Kar-Wai; Urbieta, María Sofía; Donati, Edgardo Rubén; Shamsir, Mohd S.; Goh, Kian M.
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Malaysia has a great number of hot springs, especially along the flank of the Banjaran Titiwangsa mountain range. Biological studies of the Malaysian hot springs are rare because of the lack of comprehensive information on their microbial communities. In this study, we report a cultivation-independent census to describe microbial communities in six hot springs. The Ulu Slim (US), Sungai Klah (SK), Dusun Tua (DT), Sungai Serai (SS), Semenyih (SE), and Ayer Hangat (AH) hot springs exhibit circumneutral pH with temperatures ranging from 43°C to 90°C. Genomic DNA was extracted from environmental samples and the V3-V4 hypervariable regions of 16S rRNA genes were amplified, sequenced, and analyzed. High-throughput sequencing analysis showed that microbial richness was high in all samples as indicated by the detection of 6,334-26,244 operational taxonomy units. In total, 59, 61, 72, 73, 65, and 52 bacterial phyla were identified in the US, SK, DT, SS, SE, and AH hot springs, respectively. Generally, Firmicutes and Proteobacteria dominated the bacterial communities in all hot springs. Archaeal communities mainly consisted of Crenarchaeota, Euryarchaeota, and Parvarchaeota. In beta diversity analysis, the hot spring microbial memberships were clustered primarily on the basis of temperature and salinity. Canonical correlation analysis to assess the relationship between the microbial communities and physicochemical variables revealed that diversity patterns were best explained by a combination of physicochemical variables, rather than by individual abiotic variables such as temperature and salinity.
Centro de Investigación y Desarrollo en Fermentaciones Industriales
Materia
Ciencias Exactas
16S rRNA amplicon sequencing
Hot spring metagenome
Microbial community
Microbial symbiosis
Microbiome
Saline pool
Thermophile diversity
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/87466

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network_name_str SEDICI (UNLP)
spelling Effects of physiochemical factors on prokaryotic Biodiversity in Malaysian circumneutral hot springsChan, Chia S.Chan, Kok-GanEe, RobsonHong, Kar-WaiUrbieta, María SofíaDonati, Edgardo RubénShamsir, Mohd S.Goh, Kian M.Ciencias Exactas16S rRNA amplicon sequencingHot spring metagenomeMicrobial communityMicrobial symbiosisMicrobiomeSaline poolThermophile diversityMalaysia has a great number of hot springs, especially along the flank of the Banjaran Titiwangsa mountain range. Biological studies of the Malaysian hot springs are rare because of the lack of comprehensive information on their microbial communities. In this study, we report a cultivation-independent census to describe microbial communities in six hot springs. The Ulu Slim (US), Sungai Klah (SK), Dusun Tua (DT), Sungai Serai (SS), Semenyih (SE), and Ayer Hangat (AH) hot springs exhibit circumneutral pH with temperatures ranging from 43°C to 90°C. Genomic DNA was extracted from environmental samples and the V3-V4 hypervariable regions of 16S rRNA genes were amplified, sequenced, and analyzed. High-throughput sequencing analysis showed that microbial richness was high in all samples as indicated by the detection of 6,334-26,244 operational taxonomy units. In total, 59, 61, 72, 73, 65, and 52 bacterial phyla were identified in the US, SK, DT, SS, SE, and AH hot springs, respectively. Generally, Firmicutes and Proteobacteria dominated the bacterial communities in all hot springs. Archaeal communities mainly consisted of Crenarchaeota, Euryarchaeota, and Parvarchaeota. In beta diversity analysis, the hot spring microbial memberships were clustered primarily on the basis of temperature and salinity. Canonical correlation analysis to assess the relationship between the microbial communities and physicochemical variables revealed that diversity patterns were best explained by a combination of physicochemical variables, rather than by individual abiotic variables such as temperature and salinity.Centro de Investigación y Desarrollo en Fermentaciones Industriales2017-07-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://sedici.unlp.edu.ar/handle/10915/87466enginfo:eu-repo/semantics/altIdentifier/issn/1664-302Xinfo:eu-repo/semantics/altIdentifier/doi/10.3389/fmicb.2017.01252info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:17:13Zoai:sedici.unlp.edu.ar:10915/87466Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:17:14.245SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Effects of physiochemical factors on prokaryotic Biodiversity in Malaysian circumneutral hot springs
title Effects of physiochemical factors on prokaryotic Biodiversity in Malaysian circumneutral hot springs
spellingShingle Effects of physiochemical factors on prokaryotic Biodiversity in Malaysian circumneutral hot springs
Chan, Chia S.
Ciencias Exactas
16S rRNA amplicon sequencing
Hot spring metagenome
Microbial community
Microbial symbiosis
Microbiome
Saline pool
Thermophile diversity
title_short Effects of physiochemical factors on prokaryotic Biodiversity in Malaysian circumneutral hot springs
title_full Effects of physiochemical factors on prokaryotic Biodiversity in Malaysian circumneutral hot springs
title_fullStr Effects of physiochemical factors on prokaryotic Biodiversity in Malaysian circumneutral hot springs
title_full_unstemmed Effects of physiochemical factors on prokaryotic Biodiversity in Malaysian circumneutral hot springs
title_sort Effects of physiochemical factors on prokaryotic Biodiversity in Malaysian circumneutral hot springs
dc.creator.none.fl_str_mv Chan, Chia S.
Chan, Kok-Gan
Ee, Robson
Hong, Kar-Wai
Urbieta, María Sofía
Donati, Edgardo Rubén
Shamsir, Mohd S.
Goh, Kian M.
author Chan, Chia S.
author_facet Chan, Chia S.
Chan, Kok-Gan
Ee, Robson
Hong, Kar-Wai
Urbieta, María Sofía
Donati, Edgardo Rubén
Shamsir, Mohd S.
Goh, Kian M.
author_role author
author2 Chan, Kok-Gan
Ee, Robson
Hong, Kar-Wai
Urbieta, María Sofía
Donati, Edgardo Rubén
Shamsir, Mohd S.
Goh, Kian M.
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Ciencias Exactas
16S rRNA amplicon sequencing
Hot spring metagenome
Microbial community
Microbial symbiosis
Microbiome
Saline pool
Thermophile diversity
topic Ciencias Exactas
16S rRNA amplicon sequencing
Hot spring metagenome
Microbial community
Microbial symbiosis
Microbiome
Saline pool
Thermophile diversity
dc.description.none.fl_txt_mv Malaysia has a great number of hot springs, especially along the flank of the Banjaran Titiwangsa mountain range. Biological studies of the Malaysian hot springs are rare because of the lack of comprehensive information on their microbial communities. In this study, we report a cultivation-independent census to describe microbial communities in six hot springs. The Ulu Slim (US), Sungai Klah (SK), Dusun Tua (DT), Sungai Serai (SS), Semenyih (SE), and Ayer Hangat (AH) hot springs exhibit circumneutral pH with temperatures ranging from 43°C to 90°C. Genomic DNA was extracted from environmental samples and the V3-V4 hypervariable regions of 16S rRNA genes were amplified, sequenced, and analyzed. High-throughput sequencing analysis showed that microbial richness was high in all samples as indicated by the detection of 6,334-26,244 operational taxonomy units. In total, 59, 61, 72, 73, 65, and 52 bacterial phyla were identified in the US, SK, DT, SS, SE, and AH hot springs, respectively. Generally, Firmicutes and Proteobacteria dominated the bacterial communities in all hot springs. Archaeal communities mainly consisted of Crenarchaeota, Euryarchaeota, and Parvarchaeota. In beta diversity analysis, the hot spring microbial memberships were clustered primarily on the basis of temperature and salinity. Canonical correlation analysis to assess the relationship between the microbial communities and physicochemical variables revealed that diversity patterns were best explained by a combination of physicochemical variables, rather than by individual abiotic variables such as temperature and salinity.
Centro de Investigación y Desarrollo en Fermentaciones Industriales
description Malaysia has a great number of hot springs, especially along the flank of the Banjaran Titiwangsa mountain range. Biological studies of the Malaysian hot springs are rare because of the lack of comprehensive information on their microbial communities. In this study, we report a cultivation-independent census to describe microbial communities in six hot springs. The Ulu Slim (US), Sungai Klah (SK), Dusun Tua (DT), Sungai Serai (SS), Semenyih (SE), and Ayer Hangat (AH) hot springs exhibit circumneutral pH with temperatures ranging from 43°C to 90°C. Genomic DNA was extracted from environmental samples and the V3-V4 hypervariable regions of 16S rRNA genes were amplified, sequenced, and analyzed. High-throughput sequencing analysis showed that microbial richness was high in all samples as indicated by the detection of 6,334-26,244 operational taxonomy units. In total, 59, 61, 72, 73, 65, and 52 bacterial phyla were identified in the US, SK, DT, SS, SE, and AH hot springs, respectively. Generally, Firmicutes and Proteobacteria dominated the bacterial communities in all hot springs. Archaeal communities mainly consisted of Crenarchaeota, Euryarchaeota, and Parvarchaeota. In beta diversity analysis, the hot spring microbial memberships were clustered primarily on the basis of temperature and salinity. Canonical correlation analysis to assess the relationship between the microbial communities and physicochemical variables revealed that diversity patterns were best explained by a combination of physicochemical variables, rather than by individual abiotic variables such as temperature and salinity.
publishDate 2017
dc.date.none.fl_str_mv 2017-07-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
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dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/1664-302X
info:eu-repo/semantics/altIdentifier/doi/10.3389/fmicb.2017.01252
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
eu_rights_str_mv openAccess
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Creative Commons Attribution 4.0 International (CC BY 4.0)
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