Effects of physiochemical factors on prokaryotic Biodiversity in Malaysian circumneutral hot springs
- Autores
- Chan, Chia S.; Chan, Kok-Gan; Ee, Robson; Hong, Kar-Wai; Urbieta, María Sofía; Donati, Edgardo Rubén; Shamsir, Mohd S.; Goh, Kian M.
- Año de publicación
- 2017
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Malaysia has a great number of hot springs, especially along the flank of the Banjaran Titiwangsa mountain range. Biological studies of the Malaysian hot springs are rare because of the lack of comprehensive information on their microbial communities. In this study, we report a cultivation-independent census to describe microbial communities in six hot springs. The Ulu Slim (US), Sungai Klah (SK), Dusun Tua (DT), Sungai Serai (SS), Semenyih (SE), and Ayer Hangat (AH) hot springs exhibit circumneutral pH with temperatures ranging from 43°C to 90°C. Genomic DNA was extracted from environmental samples and the V3-V4 hypervariable regions of 16S rRNA genes were amplified, sequenced, and analyzed. High-throughput sequencing analysis showed that microbial richness was high in all samples as indicated by the detection of 6,334-26,244 operational taxonomy units. In total, 59, 61, 72, 73, 65, and 52 bacterial phyla were identified in the US, SK, DT, SS, SE, and AH hot springs, respectively. Generally, Firmicutes and Proteobacteria dominated the bacterial communities in all hot springs. Archaeal communities mainly consisted of Crenarchaeota, Euryarchaeota, and Parvarchaeota. In beta diversity analysis, the hot spring microbial memberships were clustered primarily on the basis of temperature and salinity. Canonical correlation analysis to assess the relationship between the microbial communities and physicochemical variables revealed that diversity patterns were best explained by a combination of physicochemical variables, rather than by individual abiotic variables such as temperature and salinity.
Centro de Investigación y Desarrollo en Fermentaciones Industriales - Materia
-
Ciencias Exactas
16S rRNA amplicon sequencing
Hot spring metagenome
Microbial community
Microbial symbiosis
Microbiome
Saline pool
Thermophile diversity - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by/4.0/
- Repositorio
- Institución
- Universidad Nacional de La Plata
- OAI Identificador
- oai:sedici.unlp.edu.ar:10915/87466
Ver los metadatos del registro completo
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Effects of physiochemical factors on prokaryotic Biodiversity in Malaysian circumneutral hot springsChan, Chia S.Chan, Kok-GanEe, RobsonHong, Kar-WaiUrbieta, María SofíaDonati, Edgardo RubénShamsir, Mohd S.Goh, Kian M.Ciencias Exactas16S rRNA amplicon sequencingHot spring metagenomeMicrobial communityMicrobial symbiosisMicrobiomeSaline poolThermophile diversityMalaysia has a great number of hot springs, especially along the flank of the Banjaran Titiwangsa mountain range. Biological studies of the Malaysian hot springs are rare because of the lack of comprehensive information on their microbial communities. In this study, we report a cultivation-independent census to describe microbial communities in six hot springs. The Ulu Slim (US), Sungai Klah (SK), Dusun Tua (DT), Sungai Serai (SS), Semenyih (SE), and Ayer Hangat (AH) hot springs exhibit circumneutral pH with temperatures ranging from 43°C to 90°C. Genomic DNA was extracted from environmental samples and the V3-V4 hypervariable regions of 16S rRNA genes were amplified, sequenced, and analyzed. High-throughput sequencing analysis showed that microbial richness was high in all samples as indicated by the detection of 6,334-26,244 operational taxonomy units. In total, 59, 61, 72, 73, 65, and 52 bacterial phyla were identified in the US, SK, DT, SS, SE, and AH hot springs, respectively. Generally, Firmicutes and Proteobacteria dominated the bacterial communities in all hot springs. Archaeal communities mainly consisted of Crenarchaeota, Euryarchaeota, and Parvarchaeota. In beta diversity analysis, the hot spring microbial memberships were clustered primarily on the basis of temperature and salinity. Canonical correlation analysis to assess the relationship between the microbial communities and physicochemical variables revealed that diversity patterns were best explained by a combination of physicochemical variables, rather than by individual abiotic variables such as temperature and salinity.Centro de Investigación y Desarrollo en Fermentaciones Industriales2017-07-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://sedici.unlp.edu.ar/handle/10915/87466enginfo:eu-repo/semantics/altIdentifier/issn/1664-302Xinfo:eu-repo/semantics/altIdentifier/doi/10.3389/fmicb.2017.01252info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:17:13Zoai:sedici.unlp.edu.ar:10915/87466Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:17:14.245SEDICI (UNLP) - Universidad Nacional de La Platafalse |
dc.title.none.fl_str_mv |
Effects of physiochemical factors on prokaryotic Biodiversity in Malaysian circumneutral hot springs |
title |
Effects of physiochemical factors on prokaryotic Biodiversity in Malaysian circumneutral hot springs |
spellingShingle |
Effects of physiochemical factors on prokaryotic Biodiversity in Malaysian circumneutral hot springs Chan, Chia S. Ciencias Exactas 16S rRNA amplicon sequencing Hot spring metagenome Microbial community Microbial symbiosis Microbiome Saline pool Thermophile diversity |
title_short |
Effects of physiochemical factors on prokaryotic Biodiversity in Malaysian circumneutral hot springs |
title_full |
Effects of physiochemical factors on prokaryotic Biodiversity in Malaysian circumneutral hot springs |
title_fullStr |
Effects of physiochemical factors on prokaryotic Biodiversity in Malaysian circumneutral hot springs |
title_full_unstemmed |
Effects of physiochemical factors on prokaryotic Biodiversity in Malaysian circumneutral hot springs |
title_sort |
Effects of physiochemical factors on prokaryotic Biodiversity in Malaysian circumneutral hot springs |
dc.creator.none.fl_str_mv |
Chan, Chia S. Chan, Kok-Gan Ee, Robson Hong, Kar-Wai Urbieta, María Sofía Donati, Edgardo Rubén Shamsir, Mohd S. Goh, Kian M. |
author |
Chan, Chia S. |
author_facet |
Chan, Chia S. Chan, Kok-Gan Ee, Robson Hong, Kar-Wai Urbieta, María Sofía Donati, Edgardo Rubén Shamsir, Mohd S. Goh, Kian M. |
author_role |
author |
author2 |
Chan, Kok-Gan Ee, Robson Hong, Kar-Wai Urbieta, María Sofía Donati, Edgardo Rubén Shamsir, Mohd S. Goh, Kian M. |
author2_role |
author author author author author author author |
dc.subject.none.fl_str_mv |
Ciencias Exactas 16S rRNA amplicon sequencing Hot spring metagenome Microbial community Microbial symbiosis Microbiome Saline pool Thermophile diversity |
topic |
Ciencias Exactas 16S rRNA amplicon sequencing Hot spring metagenome Microbial community Microbial symbiosis Microbiome Saline pool Thermophile diversity |
dc.description.none.fl_txt_mv |
Malaysia has a great number of hot springs, especially along the flank of the Banjaran Titiwangsa mountain range. Biological studies of the Malaysian hot springs are rare because of the lack of comprehensive information on their microbial communities. In this study, we report a cultivation-independent census to describe microbial communities in six hot springs. The Ulu Slim (US), Sungai Klah (SK), Dusun Tua (DT), Sungai Serai (SS), Semenyih (SE), and Ayer Hangat (AH) hot springs exhibit circumneutral pH with temperatures ranging from 43°C to 90°C. Genomic DNA was extracted from environmental samples and the V3-V4 hypervariable regions of 16S rRNA genes were amplified, sequenced, and analyzed. High-throughput sequencing analysis showed that microbial richness was high in all samples as indicated by the detection of 6,334-26,244 operational taxonomy units. In total, 59, 61, 72, 73, 65, and 52 bacterial phyla were identified in the US, SK, DT, SS, SE, and AH hot springs, respectively. Generally, Firmicutes and Proteobacteria dominated the bacterial communities in all hot springs. Archaeal communities mainly consisted of Crenarchaeota, Euryarchaeota, and Parvarchaeota. In beta diversity analysis, the hot spring microbial memberships were clustered primarily on the basis of temperature and salinity. Canonical correlation analysis to assess the relationship between the microbial communities and physicochemical variables revealed that diversity patterns were best explained by a combination of physicochemical variables, rather than by individual abiotic variables such as temperature and salinity. Centro de Investigación y Desarrollo en Fermentaciones Industriales |
description |
Malaysia has a great number of hot springs, especially along the flank of the Banjaran Titiwangsa mountain range. Biological studies of the Malaysian hot springs are rare because of the lack of comprehensive information on their microbial communities. In this study, we report a cultivation-independent census to describe microbial communities in six hot springs. The Ulu Slim (US), Sungai Klah (SK), Dusun Tua (DT), Sungai Serai (SS), Semenyih (SE), and Ayer Hangat (AH) hot springs exhibit circumneutral pH with temperatures ranging from 43°C to 90°C. Genomic DNA was extracted from environmental samples and the V3-V4 hypervariable regions of 16S rRNA genes were amplified, sequenced, and analyzed. High-throughput sequencing analysis showed that microbial richness was high in all samples as indicated by the detection of 6,334-26,244 operational taxonomy units. In total, 59, 61, 72, 73, 65, and 52 bacterial phyla were identified in the US, SK, DT, SS, SE, and AH hot springs, respectively. Generally, Firmicutes and Proteobacteria dominated the bacterial communities in all hot springs. Archaeal communities mainly consisted of Crenarchaeota, Euryarchaeota, and Parvarchaeota. In beta diversity analysis, the hot spring microbial memberships were clustered primarily on the basis of temperature and salinity. Canonical correlation analysis to assess the relationship between the microbial communities and physicochemical variables revealed that diversity patterns were best explained by a combination of physicochemical variables, rather than by individual abiotic variables such as temperature and salinity. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-07-06 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Articulo http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
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eng |
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eng |
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