Effects of physiochemical factors on prokaryotic Biodiversity in Malaysian circumneutral hot springs

Autores
Chan, Chia S.; Chan, Kok-Gan; Ee, Robson; Hong, Kar-Wai; Urbieta, María Sofía; Donati, Edgardo Ruben; Shamsir, Mohd S.; Goh, Kian M.
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Malaysia has a great number of hot springs, especially along the flank of the Banjaran Titiwangsa mountain range. Biological studies of the Malaysian hot springs are rare because of the lack of comprehensive information on their microbial communities. In this study, we report a cultivation-independent census to describe microbial communities in six hot springs. The Ulu Slim (US), Sungai Klah (SK), Dusun Tua (DT), Sungai Serai (SS), Semenyih (SE), and Ayer Hangat (AH) hot springs exhibit circumneutral pH with temperatures ranging from 43°C to 90°C. Genomic DNA was extracted from environmental samples and the V3-V4 hypervariable regions of 16S rRNA genes were amplified, sequenced, and analyzed. High-throughput sequencing analysis showed that microbial richness was high in all samples as indicated by the detection of 6,334-26,244 operational taxonomy units. In total, 59, 61, 72, 73, 65, and 52 bacterial phyla were identified in the US, SK, DT, SS, SE, and AH hot springs, respectively. Generally, Firmicutes and Proteobacteria dominated the bacterial communities in all hot springs. Archaeal communities mainly consisted of Crenarchaeota, Euryarchaeota, and Parvarchaeota. In beta diversity analysis, the hot spring microbial memberships were clustered primarily on the basis of temperature and salinity. Canonical correlation analysis to assess the relationship between the microbial communities and physicochemical variables revealed that diversity patterns were best explained by a combination of physicochemical variables, rather than by individual abiotic variables such as temperature and salinity.
Fil: Chan, Chia S.. Universiti Teknologi Malaysia; Malasia
Fil: Chan, Kok-Gan. University of Malaya; Malasia
Fil: Ee, Robson. University of Malaya; Malasia
Fil: Hong, Kar-Wai. University of Malaya; Malasia
Fil: Urbieta, María Sofía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina
Fil: Donati, Edgardo Ruben. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina
Fil: Shamsir, Mohd S.. Universiti Teknologi Malaysia; Malasia
Fil: Goh, Kian M.. Universiti Teknologi Malaysia; Malasia
Materia
16S RRNA AMPLICON SEQUENCING
HOT SPRING METAGENOME
MICROBIAL COMMUNITY
MICROBIAL SYMBIOSIS
MICROBIOME
SALINE POOL
THERMOPHILE DIVERSITY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/48718
Acceder
id CONICETDig_c9ecd6563434532272718ddf970bb83f
oai_identifier_str oai:ri.conicet.gov.ar:11336/48718
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Effects of physiochemical factors on prokaryotic Biodiversity in Malaysian circumneutral hot springsChan, Chia S.Chan, Kok-GanEe, RobsonHong, Kar-WaiUrbieta, María SofíaDonati, Edgardo RubenShamsir, Mohd S.Goh, Kian M.16S RRNA AMPLICON SEQUENCINGHOT SPRING METAGENOMEMICROBIAL COMMUNITYMICROBIAL SYMBIOSISMICROBIOMESALINE POOLTHERMOPHILE DIVERSITYhttps://purl.org/becyt/ford/2.8https://purl.org/becyt/ford/2Malaysia has a great number of hot springs, especially along the flank of the Banjaran Titiwangsa mountain range. Biological studies of the Malaysian hot springs are rare because of the lack of comprehensive information on their microbial communities. In this study, we report a cultivation-independent census to describe microbial communities in six hot springs. The Ulu Slim (US), Sungai Klah (SK), Dusun Tua (DT), Sungai Serai (SS), Semenyih (SE), and Ayer Hangat (AH) hot springs exhibit circumneutral pH with temperatures ranging from 43°C to 90°C. Genomic DNA was extracted from environmental samples and the V3-V4 hypervariable regions of 16S rRNA genes were amplified, sequenced, and analyzed. High-throughput sequencing analysis showed that microbial richness was high in all samples as indicated by the detection of 6,334-26,244 operational taxonomy units. In total, 59, 61, 72, 73, 65, and 52 bacterial phyla were identified in the US, SK, DT, SS, SE, and AH hot springs, respectively. Generally, Firmicutes and Proteobacteria dominated the bacterial communities in all hot springs. Archaeal communities mainly consisted of Crenarchaeota, Euryarchaeota, and Parvarchaeota. In beta diversity analysis, the hot spring microbial memberships were clustered primarily on the basis of temperature and salinity. Canonical correlation analysis to assess the relationship between the microbial communities and physicochemical variables revealed that diversity patterns were best explained by a combination of physicochemical variables, rather than by individual abiotic variables such as temperature and salinity.Fil: Chan, Chia S.. Universiti Teknologi Malaysia; MalasiaFil: Chan, Kok-Gan. University of Malaya; MalasiaFil: Ee, Robson. University of Malaya; MalasiaFil: Hong, Kar-Wai. University of Malaya; MalasiaFil: Urbieta, María Sofía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; ArgentinaFil: Donati, Edgardo Ruben. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; ArgentinaFil: Shamsir, Mohd S.. Universiti Teknologi Malaysia; MalasiaFil: Goh, Kian M.. Universiti Teknologi Malaysia; MalasiaFrontiers Media S.A.2017-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/48718Chan, Chia S.; Chan, Kok-Gan; Ee, Robson; Hong, Kar-Wai; Urbieta, María Sofía; et al.; Effects of physiochemical factors on prokaryotic Biodiversity in Malaysian circumneutral hot springs; Frontiers Media S.A.; Frontiers in Microbiology; 8; 7-2017; 1-141664-302XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.3389/fmicb.2017.01252info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fmicb.2017.01252/fullinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:15:42Zoai:ri.conicet.gov.ar:11336/48718instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:15:43.014CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Effects of physiochemical factors on prokaryotic Biodiversity in Malaysian circumneutral hot springs
title Effects of physiochemical factors on prokaryotic Biodiversity in Malaysian circumneutral hot springs
spellingShingle Effects of physiochemical factors on prokaryotic Biodiversity in Malaysian circumneutral hot springs
Chan, Chia S.
16S RRNA AMPLICON SEQUENCING
HOT SPRING METAGENOME
MICROBIAL COMMUNITY
MICROBIAL SYMBIOSIS
MICROBIOME
SALINE POOL
THERMOPHILE DIVERSITY
title_short Effects of physiochemical factors on prokaryotic Biodiversity in Malaysian circumneutral hot springs
title_full Effects of physiochemical factors on prokaryotic Biodiversity in Malaysian circumneutral hot springs
title_fullStr Effects of physiochemical factors on prokaryotic Biodiversity in Malaysian circumneutral hot springs
title_full_unstemmed Effects of physiochemical factors on prokaryotic Biodiversity in Malaysian circumneutral hot springs
title_sort Effects of physiochemical factors on prokaryotic Biodiversity in Malaysian circumneutral hot springs
dc.creator.none.fl_str_mv Chan, Chia S.
Chan, Kok-Gan
Ee, Robson
Hong, Kar-Wai
Urbieta, María Sofía
Donati, Edgardo Ruben
Shamsir, Mohd S.
Goh, Kian M.
author Chan, Chia S.
author_facet Chan, Chia S.
Chan, Kok-Gan
Ee, Robson
Hong, Kar-Wai
Urbieta, María Sofía
Donati, Edgardo Ruben
Shamsir, Mohd S.
Goh, Kian M.
author_role author
author2 Chan, Kok-Gan
Ee, Robson
Hong, Kar-Wai
Urbieta, María Sofía
Donati, Edgardo Ruben
Shamsir, Mohd S.
Goh, Kian M.
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv 16S RRNA AMPLICON SEQUENCING
HOT SPRING METAGENOME
MICROBIAL COMMUNITY
MICROBIAL SYMBIOSIS
MICROBIOME
SALINE POOL
THERMOPHILE DIVERSITY
topic 16S RRNA AMPLICON SEQUENCING
HOT SPRING METAGENOME
MICROBIAL COMMUNITY
MICROBIAL SYMBIOSIS
MICROBIOME
SALINE POOL
THERMOPHILE DIVERSITY
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.8
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Malaysia has a great number of hot springs, especially along the flank of the Banjaran Titiwangsa mountain range. Biological studies of the Malaysian hot springs are rare because of the lack of comprehensive information on their microbial communities. In this study, we report a cultivation-independent census to describe microbial communities in six hot springs. The Ulu Slim (US), Sungai Klah (SK), Dusun Tua (DT), Sungai Serai (SS), Semenyih (SE), and Ayer Hangat (AH) hot springs exhibit circumneutral pH with temperatures ranging from 43°C to 90°C. Genomic DNA was extracted from environmental samples and the V3-V4 hypervariable regions of 16S rRNA genes were amplified, sequenced, and analyzed. High-throughput sequencing analysis showed that microbial richness was high in all samples as indicated by the detection of 6,334-26,244 operational taxonomy units. In total, 59, 61, 72, 73, 65, and 52 bacterial phyla were identified in the US, SK, DT, SS, SE, and AH hot springs, respectively. Generally, Firmicutes and Proteobacteria dominated the bacterial communities in all hot springs. Archaeal communities mainly consisted of Crenarchaeota, Euryarchaeota, and Parvarchaeota. In beta diversity analysis, the hot spring microbial memberships were clustered primarily on the basis of temperature and salinity. Canonical correlation analysis to assess the relationship between the microbial communities and physicochemical variables revealed that diversity patterns were best explained by a combination of physicochemical variables, rather than by individual abiotic variables such as temperature and salinity.
Fil: Chan, Chia S.. Universiti Teknologi Malaysia; Malasia
Fil: Chan, Kok-Gan. University of Malaya; Malasia
Fil: Ee, Robson. University of Malaya; Malasia
Fil: Hong, Kar-Wai. University of Malaya; Malasia
Fil: Urbieta, María Sofía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina
Fil: Donati, Edgardo Ruben. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina
Fil: Shamsir, Mohd S.. Universiti Teknologi Malaysia; Malasia
Fil: Goh, Kian M.. Universiti Teknologi Malaysia; Malasia
description Malaysia has a great number of hot springs, especially along the flank of the Banjaran Titiwangsa mountain range. Biological studies of the Malaysian hot springs are rare because of the lack of comprehensive information on their microbial communities. In this study, we report a cultivation-independent census to describe microbial communities in six hot springs. The Ulu Slim (US), Sungai Klah (SK), Dusun Tua (DT), Sungai Serai (SS), Semenyih (SE), and Ayer Hangat (AH) hot springs exhibit circumneutral pH with temperatures ranging from 43°C to 90°C. Genomic DNA was extracted from environmental samples and the V3-V4 hypervariable regions of 16S rRNA genes were amplified, sequenced, and analyzed. High-throughput sequencing analysis showed that microbial richness was high in all samples as indicated by the detection of 6,334-26,244 operational taxonomy units. In total, 59, 61, 72, 73, 65, and 52 bacterial phyla were identified in the US, SK, DT, SS, SE, and AH hot springs, respectively. Generally, Firmicutes and Proteobacteria dominated the bacterial communities in all hot springs. Archaeal communities mainly consisted of Crenarchaeota, Euryarchaeota, and Parvarchaeota. In beta diversity analysis, the hot spring microbial memberships were clustered primarily on the basis of temperature and salinity. Canonical correlation analysis to assess the relationship between the microbial communities and physicochemical variables revealed that diversity patterns were best explained by a combination of physicochemical variables, rather than by individual abiotic variables such as temperature and salinity.
publishDate 2017
dc.date.none.fl_str_mv 2017-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/48718
Chan, Chia S.; Chan, Kok-Gan; Ee, Robson; Hong, Kar-Wai; Urbieta, María Sofía; et al.; Effects of physiochemical factors on prokaryotic Biodiversity in Malaysian circumneutral hot springs; Frontiers Media S.A.; Frontiers in Microbiology; 8; 7-2017; 1-14
1664-302X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/48718
identifier_str_mv Chan, Chia S.; Chan, Kok-Gan; Ee, Robson; Hong, Kar-Wai; Urbieta, María Sofía; et al.; Effects of physiochemical factors on prokaryotic Biodiversity in Malaysian circumneutral hot springs; Frontiers Media S.A.; Frontiers in Microbiology; 8; 7-2017; 1-14
1664-302X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.3389/fmicb.2017.01252
info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fmicb.2017.01252/full
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Frontiers Media S.A.
publisher.none.fl_str_mv Frontiers Media S.A.
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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