Effects of physiochemical factors on prokaryotic Biodiversity in Malaysian circumneutral hot springs
- Autores
- Chan, Chia S.; Chan, Kok-Gan; Ee, Robson; Hong, Kar-Wai; Urbieta, María Sofía; Donati, Edgardo Ruben; Shamsir, Mohd S.; Goh, Kian M.
- Año de publicación
- 2017
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Malaysia has a great number of hot springs, especially along the flank of the Banjaran Titiwangsa mountain range. Biological studies of the Malaysian hot springs are rare because of the lack of comprehensive information on their microbial communities. In this study, we report a cultivation-independent census to describe microbial communities in six hot springs. The Ulu Slim (US), Sungai Klah (SK), Dusun Tua (DT), Sungai Serai (SS), Semenyih (SE), and Ayer Hangat (AH) hot springs exhibit circumneutral pH with temperatures ranging from 43°C to 90°C. Genomic DNA was extracted from environmental samples and the V3-V4 hypervariable regions of 16S rRNA genes were amplified, sequenced, and analyzed. High-throughput sequencing analysis showed that microbial richness was high in all samples as indicated by the detection of 6,334-26,244 operational taxonomy units. In total, 59, 61, 72, 73, 65, and 52 bacterial phyla were identified in the US, SK, DT, SS, SE, and AH hot springs, respectively. Generally, Firmicutes and Proteobacteria dominated the bacterial communities in all hot springs. Archaeal communities mainly consisted of Crenarchaeota, Euryarchaeota, and Parvarchaeota. In beta diversity analysis, the hot spring microbial memberships were clustered primarily on the basis of temperature and salinity. Canonical correlation analysis to assess the relationship between the microbial communities and physicochemical variables revealed that diversity patterns were best explained by a combination of physicochemical variables, rather than by individual abiotic variables such as temperature and salinity.
Fil: Chan, Chia S.. Universiti Teknologi Malaysia; Malasia
Fil: Chan, Kok-Gan. University of Malaya; Malasia
Fil: Ee, Robson. University of Malaya; Malasia
Fil: Hong, Kar-Wai. University of Malaya; Malasia
Fil: Urbieta, María Sofía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina
Fil: Donati, Edgardo Ruben. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina
Fil: Shamsir, Mohd S.. Universiti Teknologi Malaysia; Malasia
Fil: Goh, Kian M.. Universiti Teknologi Malaysia; Malasia - Materia
-
16S RRNA AMPLICON SEQUENCING
HOT SPRING METAGENOME
MICROBIAL COMMUNITY
MICROBIAL SYMBIOSIS
MICROBIOME
SALINE POOL
THERMOPHILE DIVERSITY - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/48718
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Effects of physiochemical factors on prokaryotic Biodiversity in Malaysian circumneutral hot springsChan, Chia S.Chan, Kok-GanEe, RobsonHong, Kar-WaiUrbieta, María SofíaDonati, Edgardo RubenShamsir, Mohd S.Goh, Kian M.16S RRNA AMPLICON SEQUENCINGHOT SPRING METAGENOMEMICROBIAL COMMUNITYMICROBIAL SYMBIOSISMICROBIOMESALINE POOLTHERMOPHILE DIVERSITYhttps://purl.org/becyt/ford/2.8https://purl.org/becyt/ford/2Malaysia has a great number of hot springs, especially along the flank of the Banjaran Titiwangsa mountain range. Biological studies of the Malaysian hot springs are rare because of the lack of comprehensive information on their microbial communities. In this study, we report a cultivation-independent census to describe microbial communities in six hot springs. The Ulu Slim (US), Sungai Klah (SK), Dusun Tua (DT), Sungai Serai (SS), Semenyih (SE), and Ayer Hangat (AH) hot springs exhibit circumneutral pH with temperatures ranging from 43°C to 90°C. Genomic DNA was extracted from environmental samples and the V3-V4 hypervariable regions of 16S rRNA genes were amplified, sequenced, and analyzed. High-throughput sequencing analysis showed that microbial richness was high in all samples as indicated by the detection of 6,334-26,244 operational taxonomy units. In total, 59, 61, 72, 73, 65, and 52 bacterial phyla were identified in the US, SK, DT, SS, SE, and AH hot springs, respectively. Generally, Firmicutes and Proteobacteria dominated the bacterial communities in all hot springs. Archaeal communities mainly consisted of Crenarchaeota, Euryarchaeota, and Parvarchaeota. In beta diversity analysis, the hot spring microbial memberships were clustered primarily on the basis of temperature and salinity. Canonical correlation analysis to assess the relationship between the microbial communities and physicochemical variables revealed that diversity patterns were best explained by a combination of physicochemical variables, rather than by individual abiotic variables such as temperature and salinity.Fil: Chan, Chia S.. Universiti Teknologi Malaysia; MalasiaFil: Chan, Kok-Gan. University of Malaya; MalasiaFil: Ee, Robson. University of Malaya; MalasiaFil: Hong, Kar-Wai. University of Malaya; MalasiaFil: Urbieta, María Sofía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; ArgentinaFil: Donati, Edgardo Ruben. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; ArgentinaFil: Shamsir, Mohd S.. Universiti Teknologi Malaysia; MalasiaFil: Goh, Kian M.. Universiti Teknologi Malaysia; MalasiaFrontiers Media S.A.2017-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/48718Chan, Chia S.; Chan, Kok-Gan; Ee, Robson; Hong, Kar-Wai; Urbieta, María Sofía; et al.; Effects of physiochemical factors on prokaryotic Biodiversity in Malaysian circumneutral hot springs; Frontiers Media S.A.; Frontiers in Microbiology; 8; 7-2017; 1-141664-302XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.3389/fmicb.2017.01252info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fmicb.2017.01252/fullinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:46:50Zoai:ri.conicet.gov.ar:11336/48718instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:46:50.769CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Effects of physiochemical factors on prokaryotic Biodiversity in Malaysian circumneutral hot springs |
title |
Effects of physiochemical factors on prokaryotic Biodiversity in Malaysian circumneutral hot springs |
spellingShingle |
Effects of physiochemical factors on prokaryotic Biodiversity in Malaysian circumneutral hot springs Chan, Chia S. 16S RRNA AMPLICON SEQUENCING HOT SPRING METAGENOME MICROBIAL COMMUNITY MICROBIAL SYMBIOSIS MICROBIOME SALINE POOL THERMOPHILE DIVERSITY |
title_short |
Effects of physiochemical factors on prokaryotic Biodiversity in Malaysian circumneutral hot springs |
title_full |
Effects of physiochemical factors on prokaryotic Biodiversity in Malaysian circumneutral hot springs |
title_fullStr |
Effects of physiochemical factors on prokaryotic Biodiversity in Malaysian circumneutral hot springs |
title_full_unstemmed |
Effects of physiochemical factors on prokaryotic Biodiversity in Malaysian circumneutral hot springs |
title_sort |
Effects of physiochemical factors on prokaryotic Biodiversity in Malaysian circumneutral hot springs |
dc.creator.none.fl_str_mv |
Chan, Chia S. Chan, Kok-Gan Ee, Robson Hong, Kar-Wai Urbieta, María Sofía Donati, Edgardo Ruben Shamsir, Mohd S. Goh, Kian M. |
author |
Chan, Chia S. |
author_facet |
Chan, Chia S. Chan, Kok-Gan Ee, Robson Hong, Kar-Wai Urbieta, María Sofía Donati, Edgardo Ruben Shamsir, Mohd S. Goh, Kian M. |
author_role |
author |
author2 |
Chan, Kok-Gan Ee, Robson Hong, Kar-Wai Urbieta, María Sofía Donati, Edgardo Ruben Shamsir, Mohd S. Goh, Kian M. |
author2_role |
author author author author author author author |
dc.subject.none.fl_str_mv |
16S RRNA AMPLICON SEQUENCING HOT SPRING METAGENOME MICROBIAL COMMUNITY MICROBIAL SYMBIOSIS MICROBIOME SALINE POOL THERMOPHILE DIVERSITY |
topic |
16S RRNA AMPLICON SEQUENCING HOT SPRING METAGENOME MICROBIAL COMMUNITY MICROBIAL SYMBIOSIS MICROBIOME SALINE POOL THERMOPHILE DIVERSITY |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/2.8 https://purl.org/becyt/ford/2 |
dc.description.none.fl_txt_mv |
Malaysia has a great number of hot springs, especially along the flank of the Banjaran Titiwangsa mountain range. Biological studies of the Malaysian hot springs are rare because of the lack of comprehensive information on their microbial communities. In this study, we report a cultivation-independent census to describe microbial communities in six hot springs. The Ulu Slim (US), Sungai Klah (SK), Dusun Tua (DT), Sungai Serai (SS), Semenyih (SE), and Ayer Hangat (AH) hot springs exhibit circumneutral pH with temperatures ranging from 43°C to 90°C. Genomic DNA was extracted from environmental samples and the V3-V4 hypervariable regions of 16S rRNA genes were amplified, sequenced, and analyzed. High-throughput sequencing analysis showed that microbial richness was high in all samples as indicated by the detection of 6,334-26,244 operational taxonomy units. In total, 59, 61, 72, 73, 65, and 52 bacterial phyla were identified in the US, SK, DT, SS, SE, and AH hot springs, respectively. Generally, Firmicutes and Proteobacteria dominated the bacterial communities in all hot springs. Archaeal communities mainly consisted of Crenarchaeota, Euryarchaeota, and Parvarchaeota. In beta diversity analysis, the hot spring microbial memberships were clustered primarily on the basis of temperature and salinity. Canonical correlation analysis to assess the relationship between the microbial communities and physicochemical variables revealed that diversity patterns were best explained by a combination of physicochemical variables, rather than by individual abiotic variables such as temperature and salinity. Fil: Chan, Chia S.. Universiti Teknologi Malaysia; Malasia Fil: Chan, Kok-Gan. University of Malaya; Malasia Fil: Ee, Robson. University of Malaya; Malasia Fil: Hong, Kar-Wai. University of Malaya; Malasia Fil: Urbieta, María Sofía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina Fil: Donati, Edgardo Ruben. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina Fil: Shamsir, Mohd S.. Universiti Teknologi Malaysia; Malasia Fil: Goh, Kian M.. Universiti Teknologi Malaysia; Malasia |
description |
Malaysia has a great number of hot springs, especially along the flank of the Banjaran Titiwangsa mountain range. Biological studies of the Malaysian hot springs are rare because of the lack of comprehensive information on their microbial communities. In this study, we report a cultivation-independent census to describe microbial communities in six hot springs. The Ulu Slim (US), Sungai Klah (SK), Dusun Tua (DT), Sungai Serai (SS), Semenyih (SE), and Ayer Hangat (AH) hot springs exhibit circumneutral pH with temperatures ranging from 43°C to 90°C. Genomic DNA was extracted from environmental samples and the V3-V4 hypervariable regions of 16S rRNA genes were amplified, sequenced, and analyzed. High-throughput sequencing analysis showed that microbial richness was high in all samples as indicated by the detection of 6,334-26,244 operational taxonomy units. In total, 59, 61, 72, 73, 65, and 52 bacterial phyla were identified in the US, SK, DT, SS, SE, and AH hot springs, respectively. Generally, Firmicutes and Proteobacteria dominated the bacterial communities in all hot springs. Archaeal communities mainly consisted of Crenarchaeota, Euryarchaeota, and Parvarchaeota. In beta diversity analysis, the hot spring microbial memberships were clustered primarily on the basis of temperature and salinity. Canonical correlation analysis to assess the relationship between the microbial communities and physicochemical variables revealed that diversity patterns were best explained by a combination of physicochemical variables, rather than by individual abiotic variables such as temperature and salinity. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-07 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/48718 Chan, Chia S.; Chan, Kok-Gan; Ee, Robson; Hong, Kar-Wai; Urbieta, María Sofía; et al.; Effects of physiochemical factors on prokaryotic Biodiversity in Malaysian circumneutral hot springs; Frontiers Media S.A.; Frontiers in Microbiology; 8; 7-2017; 1-14 1664-302X CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/48718 |
identifier_str_mv |
Chan, Chia S.; Chan, Kok-Gan; Ee, Robson; Hong, Kar-Wai; Urbieta, María Sofía; et al.; Effects of physiochemical factors on prokaryotic Biodiversity in Malaysian circumneutral hot springs; Frontiers Media S.A.; Frontiers in Microbiology; 8; 7-2017; 1-14 1664-302X CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.3389/fmicb.2017.01252 info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fmicb.2017.01252/full |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Frontiers Media S.A. |
publisher.none.fl_str_mv |
Frontiers Media S.A. |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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13.070432 |