Meta-analysis of microbial communities in hot springs: Recurrent taxa and complex shaping factors beyond ph and temperature

Autores
Massello, Francisco Luis; Chan, Chia Sing; Chan, Kok Gan; Goh, Kian Mau; Donati, Edgardo Ruben; Urbieta, María Sofía
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The study of microbial communities from extreme environments is a fascinating topic. With every study, biologists and ecologists reveal interesting facts and questions that dispel the old belief that these are inhospitable environments. In this work, we assess the microbial diversity of three hot springs from Neuquén, Argentina, using high-throughput amplicon sequencing. We predicted a distinct metabolic profile in the acidic and the circumneutral samples, with the first ones being dominated by chemolithotrophs and the second ones by chemoheterotrophs. Then, we collected data of the microbial communities of hot springs around the world in an effort to comprehend the roles of pH and temperature as shaping factors. Interestingly, there was a covariation between both parameters and the phylogenetic distance between communities; however, neither of them could explain much of the microbial profile in an ordination model. Moreover, there was no correlation between alpha diversity and these parameters. Therefore, the microbial communities’ profile seemed to have complex shaping factors beyond pH and temperature. Lastly, we looked for taxa associated with different environmental conditions. Several such taxa were found. For example, Hydrogenobaculum was frequently present in acidic springs, as was the Sulfolobaceae family; on the other hand, Candidatus Hydrothermae phylum was strongly associated with circumneutral conditions. Interestingly, some singularities related to sites featuring certain taxa were also observed.
Fil: Massello, Francisco Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina
Fil: Chan, Chia Sing. Universiti Teknologi Malaysia. Faculty of Biosciences and Medical Engineering; Malasia
Fil: Chan, Kok Gan. Universiti Teknologi Malaysia. Faculty of Biosciences and Medical Engineering; Malasia
Fil: Goh, Kian Mau. Universiti Teknologi Malaysia. Faculty of Biosciences and Medical Engineering; Malasia
Fil: Donati, Edgardo Ruben. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina
Fil: Urbieta, María Sofía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina
Materia
AMPLICON SEQUENCING
CAVIAHUE-COPAHUE
DOMUYO
EXTREME ENVIRONMENTS
EXTREMOPHILES
HOT SPRINGS
MICROBIAL COMMUNITIES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/143343
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/143343
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Meta-analysis of microbial communities in hot springs: Recurrent taxa and complex shaping factors beyond ph and temperatureMassello, Francisco LuisChan, Chia SingChan, Kok GanGoh, Kian MauDonati, Edgardo RubenUrbieta, María SofíaAMPLICON SEQUENCINGCAVIAHUE-COPAHUEDOMUYOEXTREME ENVIRONMENTSEXTREMOPHILESHOT SPRINGSMICROBIAL COMMUNITIEShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The study of microbial communities from extreme environments is a fascinating topic. With every study, biologists and ecologists reveal interesting facts and questions that dispel the old belief that these are inhospitable environments. In this work, we assess the microbial diversity of three hot springs from Neuquén, Argentina, using high-throughput amplicon sequencing. We predicted a distinct metabolic profile in the acidic and the circumneutral samples, with the first ones being dominated by chemolithotrophs and the second ones by chemoheterotrophs. Then, we collected data of the microbial communities of hot springs around the world in an effort to comprehend the roles of pH and temperature as shaping factors. Interestingly, there was a covariation between both parameters and the phylogenetic distance between communities; however, neither of them could explain much of the microbial profile in an ordination model. Moreover, there was no correlation between alpha diversity and these parameters. Therefore, the microbial communities’ profile seemed to have complex shaping factors beyond pH and temperature. Lastly, we looked for taxa associated with different environmental conditions. Several such taxa were found. For example, Hydrogenobaculum was frequently present in acidic springs, as was the Sulfolobaceae family; on the other hand, Candidatus Hydrothermae phylum was strongly associated with circumneutral conditions. Interestingly, some singularities related to sites featuring certain taxa were also observed.Fil: Massello, Francisco Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; ArgentinaFil: Chan, Chia Sing. Universiti Teknologi Malaysia. Faculty of Biosciences and Medical Engineering; MalasiaFil: Chan, Kok Gan. Universiti Teknologi Malaysia. Faculty of Biosciences and Medical Engineering; MalasiaFil: Goh, Kian Mau. Universiti Teknologi Malaysia. Faculty of Biosciences and Medical Engineering; MalasiaFil: Donati, Edgardo Ruben. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; ArgentinaFil: Urbieta, María Sofía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; ArgentinaMDPI AG2020-06-16info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/143343Massello, Francisco Luis; Chan, Chia Sing; Chan, Kok Gan; Goh, Kian Mau; Donati, Edgardo Ruben; et al.; Meta-analysis of microbial communities in hot springs: Recurrent taxa and complex shaping factors beyond ph and temperature; MDPI AG; Microorganisms; 8; 6; 16-6-2020; 1-182076-2607CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2076-2607/8/6/906info:eu-repo/semantics/altIdentifier/doi/10.3390/microorganisms8060906info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:37:49Zoai:ri.conicet.gov.ar:11336/143343instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:37:49.258CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Meta-analysis of microbial communities in hot springs: Recurrent taxa and complex shaping factors beyond ph and temperature
title Meta-analysis of microbial communities in hot springs: Recurrent taxa and complex shaping factors beyond ph and temperature
spellingShingle Meta-analysis of microbial communities in hot springs: Recurrent taxa and complex shaping factors beyond ph and temperature
Massello, Francisco Luis
AMPLICON SEQUENCING
CAVIAHUE-COPAHUE
DOMUYO
EXTREME ENVIRONMENTS
EXTREMOPHILES
HOT SPRINGS
MICROBIAL COMMUNITIES
title_short Meta-analysis of microbial communities in hot springs: Recurrent taxa and complex shaping factors beyond ph and temperature
title_full Meta-analysis of microbial communities in hot springs: Recurrent taxa and complex shaping factors beyond ph and temperature
title_fullStr Meta-analysis of microbial communities in hot springs: Recurrent taxa and complex shaping factors beyond ph and temperature
title_full_unstemmed Meta-analysis of microbial communities in hot springs: Recurrent taxa and complex shaping factors beyond ph and temperature
title_sort Meta-analysis of microbial communities in hot springs: Recurrent taxa and complex shaping factors beyond ph and temperature
dc.creator.none.fl_str_mv Massello, Francisco Luis
Chan, Chia Sing
Chan, Kok Gan
Goh, Kian Mau
Donati, Edgardo Ruben
Urbieta, María Sofía
author Massello, Francisco Luis
author_facet Massello, Francisco Luis
Chan, Chia Sing
Chan, Kok Gan
Goh, Kian Mau
Donati, Edgardo Ruben
Urbieta, María Sofía
author_role author
author2 Chan, Chia Sing
Chan, Kok Gan
Goh, Kian Mau
Donati, Edgardo Ruben
Urbieta, María Sofía
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv AMPLICON SEQUENCING
CAVIAHUE-COPAHUE
DOMUYO
EXTREME ENVIRONMENTS
EXTREMOPHILES
HOT SPRINGS
MICROBIAL COMMUNITIES
topic AMPLICON SEQUENCING
CAVIAHUE-COPAHUE
DOMUYO
EXTREME ENVIRONMENTS
EXTREMOPHILES
HOT SPRINGS
MICROBIAL COMMUNITIES
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The study of microbial communities from extreme environments is a fascinating topic. With every study, biologists and ecologists reveal interesting facts and questions that dispel the old belief that these are inhospitable environments. In this work, we assess the microbial diversity of three hot springs from Neuquén, Argentina, using high-throughput amplicon sequencing. We predicted a distinct metabolic profile in the acidic and the circumneutral samples, with the first ones being dominated by chemolithotrophs and the second ones by chemoheterotrophs. Then, we collected data of the microbial communities of hot springs around the world in an effort to comprehend the roles of pH and temperature as shaping factors. Interestingly, there was a covariation between both parameters and the phylogenetic distance between communities; however, neither of them could explain much of the microbial profile in an ordination model. Moreover, there was no correlation between alpha diversity and these parameters. Therefore, the microbial communities’ profile seemed to have complex shaping factors beyond pH and temperature. Lastly, we looked for taxa associated with different environmental conditions. Several such taxa were found. For example, Hydrogenobaculum was frequently present in acidic springs, as was the Sulfolobaceae family; on the other hand, Candidatus Hydrothermae phylum was strongly associated with circumneutral conditions. Interestingly, some singularities related to sites featuring certain taxa were also observed.
Fil: Massello, Francisco Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina
Fil: Chan, Chia Sing. Universiti Teknologi Malaysia. Faculty of Biosciences and Medical Engineering; Malasia
Fil: Chan, Kok Gan. Universiti Teknologi Malaysia. Faculty of Biosciences and Medical Engineering; Malasia
Fil: Goh, Kian Mau. Universiti Teknologi Malaysia. Faculty of Biosciences and Medical Engineering; Malasia
Fil: Donati, Edgardo Ruben. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina
Fil: Urbieta, María Sofía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina
description The study of microbial communities from extreme environments is a fascinating topic. With every study, biologists and ecologists reveal interesting facts and questions that dispel the old belief that these are inhospitable environments. In this work, we assess the microbial diversity of three hot springs from Neuquén, Argentina, using high-throughput amplicon sequencing. We predicted a distinct metabolic profile in the acidic and the circumneutral samples, with the first ones being dominated by chemolithotrophs and the second ones by chemoheterotrophs. Then, we collected data of the microbial communities of hot springs around the world in an effort to comprehend the roles of pH and temperature as shaping factors. Interestingly, there was a covariation between both parameters and the phylogenetic distance between communities; however, neither of them could explain much of the microbial profile in an ordination model. Moreover, there was no correlation between alpha diversity and these parameters. Therefore, the microbial communities’ profile seemed to have complex shaping factors beyond pH and temperature. Lastly, we looked for taxa associated with different environmental conditions. Several such taxa were found. For example, Hydrogenobaculum was frequently present in acidic springs, as was the Sulfolobaceae family; on the other hand, Candidatus Hydrothermae phylum was strongly associated with circumneutral conditions. Interestingly, some singularities related to sites featuring certain taxa were also observed.
publishDate 2020
dc.date.none.fl_str_mv 2020-06-16
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/143343
Massello, Francisco Luis; Chan, Chia Sing; Chan, Kok Gan; Goh, Kian Mau; Donati, Edgardo Ruben; et al.; Meta-analysis of microbial communities in hot springs: Recurrent taxa and complex shaping factors beyond ph and temperature; MDPI AG; Microorganisms; 8; 6; 16-6-2020; 1-18
2076-2607
CONICET Digital
CONICET
url http://hdl.handle.net/11336/143343
identifier_str_mv Massello, Francisco Luis; Chan, Chia Sing; Chan, Kok Gan; Goh, Kian Mau; Donati, Edgardo Ruben; et al.; Meta-analysis of microbial communities in hot springs: Recurrent taxa and complex shaping factors beyond ph and temperature; MDPI AG; Microorganisms; 8; 6; 16-6-2020; 1-18
2076-2607
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2076-2607/8/6/906
info:eu-repo/semantics/altIdentifier/doi/10.3390/microorganisms8060906
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI AG
publisher.none.fl_str_mv MDPI AG
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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