Supplementary material for "A Synthetic Microbial Community for Soybean Biofertilization Designed via Chlorophyll-Based Iterative Selection"

Autores
Brignoli, Damián; Colla, Delfina; Frickel Critto, Emilia Josefina; Castells, Cecilia Beatriz Marta; Pérez Giménez, Julieta; Lodeiro, Aníbal Roberto
Año de publicación
2026
Idioma
inglés
Tipo de recurso
conjunto de datos
Estado
versión publicada
Descripción
Improving the effectiveness of microbial inoculants for soybean is essential to enhance biological nitrogen fixation and reduce fertilizer dependence; however, inoculated Bradyrhizobium strains frequently display inconsistent field performance. Inoculation is usually carried out with single-strain formulations, overlooking the possible influence of the native soil microbiota on nodulation success. This limitation may be addressed by formulating inoculants with consortia that include selected members of the soil microbiota. To this end, a synthetic microbial community (SynCom) was developed through a host-mediated microbiome engineering approach guided by leaf chlorophyll content as a rapid, non-destructive plant trait. The experiment was initiated by inoculating soybean plants with a consortium of 9 Bradyrhizobium spp. and 14 non-rhizobial soil isolates. Across eight consecutive selection rounds under gnotobiotic conditions, rhizosphere communities associated with superior plant performance were pooled and propagated. Recurrent selection induced significant shifts in community composition, consistently favoring B. diazoefficiens as the dominant nodulating member and enriching taxa from Pseudomonadales, Burkholderiales, and Sphingomonadales. Sequencing-based profiling and network analysis suggested the emergence of a cohesive and functionally enriched community, with increased potential for nitrogen transformations and organic matter turnover. When evaluated in non-sterile soil, the SynCom derived from the sixth selection round increased nodule number and biomass relative to an uninoculated control and a commercial inoculant strain. These results suggest that plant-guided selection can steer rhizosphere community assembly toward beneficial configurations and support the development of improved soybean bioinoculants.
Fil: Brignoli, Damián. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular. Argentina Fil: Colla, Delfina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular. Argentina Fil: Frickel Critto, Emilia Josefina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular. Argentina Fil: Castells, Cecilia Beatriz Marta. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular. Argentina Fil: Pérez Giménez, Julieta, Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular. Argentina Fil: Lodeiro, Aníbal Roberto. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular. Argentina
Instituto de Biotecnología y Biología Molecular
Materia
Ciencias Exactas
Bradyrhizobium
Plant growth-promoting rhizobacteria (PGPR)
nitrogen fixation
rhizosphere
microbiome
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/191256
Acceder
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network_name_str SEDICI (UNLP)
spelling Supplementary material for "A Synthetic Microbial Community for Soybean Biofertilization Designed via Chlorophyll-Based Iterative Selection"Brignoli, DamiánColla, DelfinaFrickel Critto, Emilia JosefinaCastells, Cecilia Beatriz MartaPérez Giménez, JulietaLodeiro, Aníbal RobertoCiencias Exactashttps://purl.org/becyt/ford/1.6BradyrhizobiumPlant growth-promoting rhizobacteria (PGPR)nitrogen fixationrhizospheremicrobiomeImproving the effectiveness of microbial inoculants for soybean is essential to enhance biological nitrogen fixation and reduce fertilizer dependence; however, inoculated Bradyrhizobium strains frequently display inconsistent field performance. Inoculation is usually carried out with single-strain formulations, overlooking the possible influence of the native soil microbiota on nodulation success. This limitation may be addressed by formulating inoculants with consortia that include selected members of the soil microbiota. To this end, a synthetic microbial community (SynCom) was developed through a host-mediated microbiome engineering approach guided by leaf chlorophyll content as a rapid, non-destructive plant trait. The experiment was initiated by inoculating soybean plants with a consortium of 9 Bradyrhizobium spp. and 14 non-rhizobial soil isolates. Across eight consecutive selection rounds under gnotobiotic conditions, rhizosphere communities associated with superior plant performance were pooled and propagated. Recurrent selection induced significant shifts in community composition, consistently favoring B. diazoefficiens as the dominant nodulating member and enriching taxa from Pseudomonadales, Burkholderiales, and Sphingomonadales. Sequencing-based profiling and network analysis suggested the emergence of a cohesive and functionally enriched community, with increased potential for nitrogen transformations and organic matter turnover. When evaluated in non-sterile soil, the SynCom derived from the sixth selection round increased nodule number and biomass relative to an uninoculated control and a commercial inoculant strain. These results suggest that plant-guided selection can steer rhizosphere community assembly toward beneficial configurations and support the development of improved soybean bioinoculants.Fil: Brignoli, Damián. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular. Argentina Fil: Colla, Delfina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular. Argentina Fil: Frickel Critto, Emilia Josefina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular. Argentina Fil: Castells, Cecilia Beatriz Marta. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular. Argentina Fil: Pérez Giménez, Julieta, Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular. Argentina Fil: Lodeiro, Aníbal Roberto. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular. ArgentinaInstituto de Biotecnología y Biología Molecular2026-03-02info:eu-repo/semantics/publishedVersionConjunto de datoshttp://purl.org/coar/resource_type/c_ddb1info:ar-repo/semantics/conjuntoDeDatosinfo:eu-repo/semantics/dataSetapplication/pdfPCR, secuenciación de ADN, análisis bioinformático de las secuenciashttp://sedici.unlp.edu.ar/handle/10915/191256https://doi.org/10.35537/10915/191256enginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2026-03-26T09:21:33Zoai:sedici.unlp.edu.ar:10915/191256Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292026-03-26 09:21:33.338SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Supplementary material for "A Synthetic Microbial Community for Soybean Biofertilization Designed via Chlorophyll-Based Iterative Selection"
title Supplementary material for "A Synthetic Microbial Community for Soybean Biofertilization Designed via Chlorophyll-Based Iterative Selection"
spellingShingle Supplementary material for "A Synthetic Microbial Community for Soybean Biofertilization Designed via Chlorophyll-Based Iterative Selection"
Brignoli, Damián
Ciencias Exactas
Bradyrhizobium
Plant growth-promoting rhizobacteria (PGPR)
nitrogen fixation
rhizosphere
microbiome
title_short Supplementary material for "A Synthetic Microbial Community for Soybean Biofertilization Designed via Chlorophyll-Based Iterative Selection"
title_full Supplementary material for "A Synthetic Microbial Community for Soybean Biofertilization Designed via Chlorophyll-Based Iterative Selection"
title_fullStr Supplementary material for "A Synthetic Microbial Community for Soybean Biofertilization Designed via Chlorophyll-Based Iterative Selection"
title_full_unstemmed Supplementary material for "A Synthetic Microbial Community for Soybean Biofertilization Designed via Chlorophyll-Based Iterative Selection"
title_sort Supplementary material for "A Synthetic Microbial Community for Soybean Biofertilization Designed via Chlorophyll-Based Iterative Selection"
dc.creator.none.fl_str_mv Brignoli, Damián
Colla, Delfina
Frickel Critto, Emilia Josefina
Castells, Cecilia Beatriz Marta
Pérez Giménez, Julieta
Lodeiro, Aníbal Roberto
author Brignoli, Damián
author_facet Brignoli, Damián
Colla, Delfina
Frickel Critto, Emilia Josefina
Castells, Cecilia Beatriz Marta
Pérez Giménez, Julieta
Lodeiro, Aníbal Roberto
author_role author
author2 Colla, Delfina
Frickel Critto, Emilia Josefina
Castells, Cecilia Beatriz Marta
Pérez Giménez, Julieta
Lodeiro, Aníbal Roberto
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Ciencias Exactas
Bradyrhizobium
Plant growth-promoting rhizobacteria (PGPR)
nitrogen fixation
rhizosphere
microbiome
topic Ciencias Exactas
Bradyrhizobium
Plant growth-promoting rhizobacteria (PGPR)
nitrogen fixation
rhizosphere
microbiome
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
dc.description.none.fl_txt_mv Improving the effectiveness of microbial inoculants for soybean is essential to enhance biological nitrogen fixation and reduce fertilizer dependence; however, inoculated Bradyrhizobium strains frequently display inconsistent field performance. Inoculation is usually carried out with single-strain formulations, overlooking the possible influence of the native soil microbiota on nodulation success. This limitation may be addressed by formulating inoculants with consortia that include selected members of the soil microbiota. To this end, a synthetic microbial community (SynCom) was developed through a host-mediated microbiome engineering approach guided by leaf chlorophyll content as a rapid, non-destructive plant trait. The experiment was initiated by inoculating soybean plants with a consortium of 9 Bradyrhizobium spp. and 14 non-rhizobial soil isolates. Across eight consecutive selection rounds under gnotobiotic conditions, rhizosphere communities associated with superior plant performance were pooled and propagated. Recurrent selection induced significant shifts in community composition, consistently favoring B. diazoefficiens as the dominant nodulating member and enriching taxa from Pseudomonadales, Burkholderiales, and Sphingomonadales. Sequencing-based profiling and network analysis suggested the emergence of a cohesive and functionally enriched community, with increased potential for nitrogen transformations and organic matter turnover. When evaluated in non-sterile soil, the SynCom derived from the sixth selection round increased nodule number and biomass relative to an uninoculated control and a commercial inoculant strain. These results suggest that plant-guided selection can steer rhizosphere community assembly toward beneficial configurations and support the development of improved soybean bioinoculants.
Fil: Brignoli, Damián. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular. Argentina Fil: Colla, Delfina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular. Argentina Fil: Frickel Critto, Emilia Josefina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular. Argentina Fil: Castells, Cecilia Beatriz Marta. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular. Argentina Fil: Pérez Giménez, Julieta, Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular. Argentina Fil: Lodeiro, Aníbal Roberto. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular. Argentina
Instituto de Biotecnología y Biología Molecular
description Improving the effectiveness of microbial inoculants for soybean is essential to enhance biological nitrogen fixation and reduce fertilizer dependence; however, inoculated Bradyrhizobium strains frequently display inconsistent field performance. Inoculation is usually carried out with single-strain formulations, overlooking the possible influence of the native soil microbiota on nodulation success. This limitation may be addressed by formulating inoculants with consortia that include selected members of the soil microbiota. To this end, a synthetic microbial community (SynCom) was developed through a host-mediated microbiome engineering approach guided by leaf chlorophyll content as a rapid, non-destructive plant trait. The experiment was initiated by inoculating soybean plants with a consortium of 9 Bradyrhizobium spp. and 14 non-rhizobial soil isolates. Across eight consecutive selection rounds under gnotobiotic conditions, rhizosphere communities associated with superior plant performance were pooled and propagated. Recurrent selection induced significant shifts in community composition, consistently favoring B. diazoefficiens as the dominant nodulating member and enriching taxa from Pseudomonadales, Burkholderiales, and Sphingomonadales. Sequencing-based profiling and network analysis suggested the emergence of a cohesive and functionally enriched community, with increased potential for nitrogen transformations and organic matter turnover. When evaluated in non-sterile soil, the SynCom derived from the sixth selection round increased nodule number and biomass relative to an uninoculated control and a commercial inoculant strain. These results suggest that plant-guided selection can steer rhizosphere community assembly toward beneficial configurations and support the development of improved soybean bioinoculants.
publishDate 2026
dc.date.none.fl_str_mv 2026-03-02
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
Conjunto de datos
http://purl.org/coar/resource_type/c_ddb1
info:ar-repo/semantics/conjuntoDeDatos
info:eu-repo/semantics/dataSet
status_str publishedVersion
format dataSet
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/191256
https://doi.org/10.35537/10915/191256
url http://sedici.unlp.edu.ar/handle/10915/191256
https://doi.org/10.35537/10915/191256
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
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Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
PCR, secuenciación de ADN, análisis bioinformático de las secuencias
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repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
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