Response of alfalfa (<i>Medicago sativa</i> L.) to single and mixed inoculation with phosphate-solubilizing bacteria and <i>Sinorhizobium meliloti</i>

Autores
Guiñazú, Lorena Belén; Andrés, Javier Alberto; Del Papa, María Florencia; Pistorio, Mariano; Rosas, Susana Beatriz
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The objectives of this work were to phenotypically and genetically characterize alfalfa rhizosphere bacteria and to evaluate the effect of single or mixed inoculation upon nodulation and biological nitrogen fixation. Thirty-two strains showed tricalcium phosphate solubilization ability, and two of them caused bigger or equal solubilization halos than the control strain P. putida SP22. The comparison of the 16S ribosomal DNA sequences indicated that these strains are phylogenetically related to Bacillus spp. and Pseudomonas spp. A beneficial effect of both isolates on alfalfa growth was observed in coinoculation assays. Pseudomonas sp. FM7d caused a significant increase in root and shoot dry weight, length, and surface area of roots, number, and symbiotic properties of alfalfa plants. The plants coinoculated with Sinorhizobium meliloti B399 and the Bacillus sp. M7c showed significant increases in the measured parameters. Our results indicating that strains Pseudomonas sp. FM7d and Bacillus sp. M7c can be considered for the formulation of new inoculants.
Facultad de Ciencias Exactas
Instituto de Biotecnología y Biología Molecular
Materia
Biología
alfalfa
Plant growth-promoting rhizobacteria
Coinoculation
Biological nitrogen fixation
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/134058
Acceder
id SEDICI_7a7413b4023dcd9734e09d7c61833eaf
oai_identifier_str oai:sedici.unlp.edu.ar:10915/134058
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Response of alfalfa (<i>Medicago sativa</i> L.) to single and mixed inoculation with phosphate-solubilizing bacteria and <i>Sinorhizobium meliloti</i>Guiñazú, Lorena BelénAndrés, Javier AlbertoDel Papa, María FlorenciaPistorio, MarianoRosas, Susana BeatrizBiologíaalfalfaPlant growth-promoting rhizobacteriaCoinoculationBiological nitrogen fixationThe objectives of this work were to phenotypically and genetically characterize alfalfa rhizosphere bacteria and to evaluate the effect of single or mixed inoculation upon nodulation and biological nitrogen fixation. Thirty-two strains showed tricalcium phosphate solubilization ability, and two of them caused bigger or equal solubilization halos than the control strain <i>P. putida</i> SP22. The comparison of the 16S ribosomal DNA sequences indicated that these strains are phylogenetically related to <i>Bacillus</i> spp. and <i>Pseudomonas</i> spp. A beneficial effect of both isolates on alfalfa growth was observed in coinoculation assays. <i>Pseudomonas</i> sp. FM7d caused a significant increase in root and shoot dry weight, length, and surface area of roots, number, and symbiotic properties of alfalfa plants. The plants coinoculated with <i>Sinorhizobium meliloti</i> B399 and the <i>Bacillus</i> sp. M7c showed significant increases in the measured parameters. Our results indicating that strains <i>Pseudomonas</i> sp. FM7d and <i>Bacillus</i> sp. M7c can be considered for the formulation of new inoculants.Facultad de Ciencias ExactasInstituto de Biotecnología y Biología Molecular2010-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf185-190http://sedici.unlp.edu.ar/handle/10915/134058enginfo:eu-repo/semantics/altIdentifier/issn/0178-2762info:eu-repo/semantics/altIdentifier/issn/1432-0789info:eu-repo/semantics/altIdentifier/doi/10.1007/s00374-009-0408-5info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-10-22T17:12:51Zoai:sedici.unlp.edu.ar:10915/134058Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-10-22 17:12:51.598SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Response of alfalfa (<i>Medicago sativa</i> L.) to single and mixed inoculation with phosphate-solubilizing bacteria and <i>Sinorhizobium meliloti</i>
title Response of alfalfa (<i>Medicago sativa</i> L.) to single and mixed inoculation with phosphate-solubilizing bacteria and <i>Sinorhizobium meliloti</i>
spellingShingle Response of alfalfa (<i>Medicago sativa</i> L.) to single and mixed inoculation with phosphate-solubilizing bacteria and <i>Sinorhizobium meliloti</i>
Guiñazú, Lorena Belén
Biología
alfalfa
Plant growth-promoting rhizobacteria
Coinoculation
Biological nitrogen fixation
title_short Response of alfalfa (<i>Medicago sativa</i> L.) to single and mixed inoculation with phosphate-solubilizing bacteria and <i>Sinorhizobium meliloti</i>
title_full Response of alfalfa (<i>Medicago sativa</i> L.) to single and mixed inoculation with phosphate-solubilizing bacteria and <i>Sinorhizobium meliloti</i>
title_fullStr Response of alfalfa (<i>Medicago sativa</i> L.) to single and mixed inoculation with phosphate-solubilizing bacteria and <i>Sinorhizobium meliloti</i>
title_full_unstemmed Response of alfalfa (<i>Medicago sativa</i> L.) to single and mixed inoculation with phosphate-solubilizing bacteria and <i>Sinorhizobium meliloti</i>
title_sort Response of alfalfa (<i>Medicago sativa</i> L.) to single and mixed inoculation with phosphate-solubilizing bacteria and <i>Sinorhizobium meliloti</i>
dc.creator.none.fl_str_mv Guiñazú, Lorena Belén
Andrés, Javier Alberto
Del Papa, María Florencia
Pistorio, Mariano
Rosas, Susana Beatriz
author Guiñazú, Lorena Belén
author_facet Guiñazú, Lorena Belén
Andrés, Javier Alberto
Del Papa, María Florencia
Pistorio, Mariano
Rosas, Susana Beatriz
author_role author
author2 Andrés, Javier Alberto
Del Papa, María Florencia
Pistorio, Mariano
Rosas, Susana Beatriz
author2_role author
author
author
author
dc.subject.none.fl_str_mv Biología
alfalfa
Plant growth-promoting rhizobacteria
Coinoculation
Biological nitrogen fixation
topic Biología
alfalfa
Plant growth-promoting rhizobacteria
Coinoculation
Biological nitrogen fixation
dc.description.none.fl_txt_mv The objectives of this work were to phenotypically and genetically characterize alfalfa rhizosphere bacteria and to evaluate the effect of single or mixed inoculation upon nodulation and biological nitrogen fixation. Thirty-two strains showed tricalcium phosphate solubilization ability, and two of them caused bigger or equal solubilization halos than the control strain <i>P. putida</i> SP22. The comparison of the 16S ribosomal DNA sequences indicated that these strains are phylogenetically related to <i>Bacillus</i> spp. and <i>Pseudomonas</i> spp. A beneficial effect of both isolates on alfalfa growth was observed in coinoculation assays. <i>Pseudomonas</i> sp. FM7d caused a significant increase in root and shoot dry weight, length, and surface area of roots, number, and symbiotic properties of alfalfa plants. The plants coinoculated with <i>Sinorhizobium meliloti</i> B399 and the <i>Bacillus</i> sp. M7c showed significant increases in the measured parameters. Our results indicating that strains <i>Pseudomonas</i> sp. FM7d and <i>Bacillus</i> sp. M7c can be considered for the formulation of new inoculants.
Facultad de Ciencias Exactas
Instituto de Biotecnología y Biología Molecular
description The objectives of this work were to phenotypically and genetically characterize alfalfa rhizosphere bacteria and to evaluate the effect of single or mixed inoculation upon nodulation and biological nitrogen fixation. Thirty-two strains showed tricalcium phosphate solubilization ability, and two of them caused bigger or equal solubilization halos than the control strain <i>P. putida</i> SP22. The comparison of the 16S ribosomal DNA sequences indicated that these strains are phylogenetically related to <i>Bacillus</i> spp. and <i>Pseudomonas</i> spp. A beneficial effect of both isolates on alfalfa growth was observed in coinoculation assays. <i>Pseudomonas</i> sp. FM7d caused a significant increase in root and shoot dry weight, length, and surface area of roots, number, and symbiotic properties of alfalfa plants. The plants coinoculated with <i>Sinorhizobium meliloti</i> B399 and the <i>Bacillus</i> sp. M7c showed significant increases in the measured parameters. Our results indicating that strains <i>Pseudomonas</i> sp. FM7d and <i>Bacillus</i> sp. M7c can be considered for the formulation of new inoculants.
publishDate 2010
dc.date.none.fl_str_mv 2010-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/134058
url http://sedici.unlp.edu.ar/handle/10915/134058
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/0178-2762
info:eu-repo/semantics/altIdentifier/issn/1432-0789
info:eu-repo/semantics/altIdentifier/doi/10.1007/s00374-009-0408-5
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
dc.format.none.fl_str_mv application/pdf
185-190
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
_version_ 1846783492863557632
score 12.982451

Publicaciones similares