The gap gene Krüppel of Rhodnius prolixus is required for segmentation and for repression of the homeotic gene sex comb-reduced

Autores
Lavore, Andrés E.; Esponda Behrens, Natalia; Pagola, Lucía Elena; Rivera Pomar, Rolando Víctor
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The establishment of the anterior-posterior segmentation in insects requires the concerted action of a hierarchical gene network. Here, we study the orthologue of Krüppel gap gene in the hemipteran Rhodnius prolixus (Rp-Kr). We characterized its structure, expression pattern and function. The genomic sequence upstream of the Rp-Kr transcriptional unit shows a putative regulatory region conserved in the orthologue genes from Drosophila melanogaster and Tribolium castaneum. Rp-Kr expression is zygotic and it is expressed in the anterior half of the embryo (the posterior half of the egg) during the blastoderm stage and germ band formation; later, during germ band extension, it is expressed in a central domain, from T2 to A3. The Rp-Kr loss of function phenotypes shows disrupted thoracic and abdominal segmentation. Embryos with weak segmentation phenotypes show homeotic transformations, in which an ectopic tibial comb, typical of T1 leg, appears in T2, which correlates with the ectopic expression of Rp-sex-comb reduced in this leg.
Facultad de Ciencias Exactas
Materia
Ciencias Exactas
Gap gene
Hox regulation
Krüppel
Rhodnius
Segmentation
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/85060
Acceder
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oai_identifier_str oai:sedici.unlp.edu.ar:10915/85060
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repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling The gap gene Krüppel of Rhodnius prolixus is required for segmentation and for repression of the homeotic gene sex comb-reducedLavore, Andrés E.Esponda Behrens, NataliaPagola, Lucía ElenaRivera Pomar, Rolando VíctorCiencias ExactasGap geneHox regulationKrüppelRhodniusSegmentationThe establishment of the anterior-posterior segmentation in insects requires the concerted action of a hierarchical gene network. Here, we study the orthologue of <i>Krüppel</i> gap gene in the hemipteran <i>Rhodnius prolixus</i> (<i>Rp</i>-<i>Kr</i>). We characterized its structure, expression pattern and function. The genomic sequence upstream of the <i>Rp</i>-<i>Kr</i> transcriptional unit shows a putative regulatory region conserved in the orthologue genes from <i>Drosophila melanogaster</i> and <i>Tribolium castaneum</i>. <i>Rp</i>-<i>Kr</i> expression is zygotic and it is expressed in the anterior half of the embryo (the posterior half of the egg) during the blastoderm stage and germ band formation; later, during germ band extension, it is expressed in a central domain, from T2 to A3. The <i>Rp</i>-<i>Kr</i> loss of function phenotypes shows disrupted thoracic and abdominal segmentation. Embryos with weak segmentation phenotypes show homeotic transformations, in which an ectopic tibial comb, typical of T1 leg, appears in T2, which correlates with the ectopic expression of <i>Rp-sex-comb reduced</i> in this leg.Facultad de Ciencias Exactas2014info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf121-129http://sedici.unlp.edu.ar/handle/10915/85060enginfo:eu-repo/semantics/altIdentifier/issn/0012-1606info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ydbio.2013.12.030info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:16:24Zoai:sedici.unlp.edu.ar:10915/85060Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:16:25.046SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv The gap gene Krüppel of Rhodnius prolixus is required for segmentation and for repression of the homeotic gene sex comb-reduced
title The gap gene Krüppel of Rhodnius prolixus is required for segmentation and for repression of the homeotic gene sex comb-reduced
spellingShingle The gap gene Krüppel of Rhodnius prolixus is required for segmentation and for repression of the homeotic gene sex comb-reduced
Lavore, Andrés E.
Ciencias Exactas
Gap gene
Hox regulation
Krüppel
Rhodnius
Segmentation
title_short The gap gene Krüppel of Rhodnius prolixus is required for segmentation and for repression of the homeotic gene sex comb-reduced
title_full The gap gene Krüppel of Rhodnius prolixus is required for segmentation and for repression of the homeotic gene sex comb-reduced
title_fullStr The gap gene Krüppel of Rhodnius prolixus is required for segmentation and for repression of the homeotic gene sex comb-reduced
title_full_unstemmed The gap gene Krüppel of Rhodnius prolixus is required for segmentation and for repression of the homeotic gene sex comb-reduced
title_sort The gap gene Krüppel of Rhodnius prolixus is required for segmentation and for repression of the homeotic gene sex comb-reduced
dc.creator.none.fl_str_mv Lavore, Andrés E.
Esponda Behrens, Natalia
Pagola, Lucía Elena
Rivera Pomar, Rolando Víctor
author Lavore, Andrés E.
author_facet Lavore, Andrés E.
Esponda Behrens, Natalia
Pagola, Lucía Elena
Rivera Pomar, Rolando Víctor
author_role author
author2 Esponda Behrens, Natalia
Pagola, Lucía Elena
Rivera Pomar, Rolando Víctor
author2_role author
author
author
dc.subject.none.fl_str_mv Ciencias Exactas
Gap gene
Hox regulation
Krüppel
Rhodnius
Segmentation
topic Ciencias Exactas
Gap gene
Hox regulation
Krüppel
Rhodnius
Segmentation
dc.description.none.fl_txt_mv The establishment of the anterior-posterior segmentation in insects requires the concerted action of a hierarchical gene network. Here, we study the orthologue of <i>Krüppel</i> gap gene in the hemipteran <i>Rhodnius prolixus</i> (<i>Rp</i>-<i>Kr</i>). We characterized its structure, expression pattern and function. The genomic sequence upstream of the <i>Rp</i>-<i>Kr</i> transcriptional unit shows a putative regulatory region conserved in the orthologue genes from <i>Drosophila melanogaster</i> and <i>Tribolium castaneum</i>. <i>Rp</i>-<i>Kr</i> expression is zygotic and it is expressed in the anterior half of the embryo (the posterior half of the egg) during the blastoderm stage and germ band formation; later, during germ band extension, it is expressed in a central domain, from T2 to A3. The <i>Rp</i>-<i>Kr</i> loss of function phenotypes shows disrupted thoracic and abdominal segmentation. Embryos with weak segmentation phenotypes show homeotic transformations, in which an ectopic tibial comb, typical of T1 leg, appears in T2, which correlates with the ectopic expression of <i>Rp-sex-comb reduced</i> in this leg.
Facultad de Ciencias Exactas
description The establishment of the anterior-posterior segmentation in insects requires the concerted action of a hierarchical gene network. Here, we study the orthologue of <i>Krüppel</i> gap gene in the hemipteran <i>Rhodnius prolixus</i> (<i>Rp</i>-<i>Kr</i>). We characterized its structure, expression pattern and function. The genomic sequence upstream of the <i>Rp</i>-<i>Kr</i> transcriptional unit shows a putative regulatory region conserved in the orthologue genes from <i>Drosophila melanogaster</i> and <i>Tribolium castaneum</i>. <i>Rp</i>-<i>Kr</i> expression is zygotic and it is expressed in the anterior half of the embryo (the posterior half of the egg) during the blastoderm stage and germ band formation; later, during germ band extension, it is expressed in a central domain, from T2 to A3. The <i>Rp</i>-<i>Kr</i> loss of function phenotypes shows disrupted thoracic and abdominal segmentation. Embryos with weak segmentation phenotypes show homeotic transformations, in which an ectopic tibial comb, typical of T1 leg, appears in T2, which correlates with the ectopic expression of <i>Rp-sex-comb reduced</i> in this leg.
publishDate 2014
dc.date.none.fl_str_mv 2014
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/85060
url http://sedici.unlp.edu.ar/handle/10915/85060
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/0012-1606
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ydbio.2013.12.030
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
121-129
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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score 13.070432

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