The gap gene giant of Rhodnius prolixus is maternally expressed and required for proper head and abdomen formation

Autores
Lavore, Andres Esteban; Pagola, Lucia Elena; Esponda Behrens, Natalia Irene; Rivera Pomar, Rolando
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The segmentation process in insects depends on a hierarchical cascade of gene activity. The first effectors downstream of the maternal activation are the gap genes, which divide the embryo in broad fields. We discovered a sequence corresponding to the leucine-zipper domain of the orthologue of the gene giant (Rp- gt) in traces from the genome of Rhodnius prolixus, a hemipteran with intermediate germ-band development. We cloned the Rp- gt gene from a normalized cDNA library and characterized its expression and function. Bioinformatic analysis of 12.5. kbp of genomic sequence containing the Rp- gt transcriptional unit shows a cluster of bona fide regulatory binding sites, which is similar in location and structure to the predicted posterior expression domain of the Drosophila orthologue. Rp-gt is expressed in ovaries and maternally supplied in the early embryo. The maternal contribution forms a gradient of scattered patches of mRNA in the preblastoderm embryo. Zygotic Rp-gt is expressed in two domains that after germ band extension are restricted to the head and the posterior growth zone. Parental RNAi shows that Rp-gt is required for proper head and abdomen formation. The head lacks mandibulary and maxillary appendages and shows reduced clypeus-labrum, while the abdomen lacks anterior segments. We conclude that Rp-gt is a gap gene on the head and abdomen and, in addition, has a function in patterning the anterior head capsule suggesting that the function of gt in hemipterans is more similar to dipterans than expected. © 2011.
Fil: Lavore, Andres Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Centro Regional de Estudios Genómicos; Argentina
Fil: Pagola, Lucia Elena. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Centro Regional de Estudios Genómicos; Argentina
Fil: Esponda Behrens, Natalia Irene. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Centro Regional de Estudios Genómicos; Argentina
Fil: Rivera Pomar, Rolando. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Centro Regional de Estudios Genómicos; Argentina. Universidad Nacional del Noroeste de la Provincia de Buenos Aires. Departamento de Ciencias Básicas y Experimentales; Argentina
Materia
Gap Gene
Giant
Maternal Expression
Regulatory Cluster
Rhodnius
Segmentation
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/76027
Acceder
id CONICETDig_32a2257a080e7631fa26d4d12d5f50b7
oai_identifier_str oai:ri.conicet.gov.ar:11336/76027
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling The gap gene giant of Rhodnius prolixus is maternally expressed and required for proper head and abdomen formationLavore, Andres EstebanPagola, Lucia ElenaEsponda Behrens, Natalia IreneRivera Pomar, RolandoGap GeneGiantMaternal ExpressionRegulatory ClusterRhodniusSegmentationhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The segmentation process in insects depends on a hierarchical cascade of gene activity. The first effectors downstream of the maternal activation are the gap genes, which divide the embryo in broad fields. We discovered a sequence corresponding to the leucine-zipper domain of the orthologue of the gene giant (Rp- gt) in traces from the genome of Rhodnius prolixus, a hemipteran with intermediate germ-band development. We cloned the Rp- gt gene from a normalized cDNA library and characterized its expression and function. Bioinformatic analysis of 12.5. kbp of genomic sequence containing the Rp- gt transcriptional unit shows a cluster of bona fide regulatory binding sites, which is similar in location and structure to the predicted posterior expression domain of the Drosophila orthologue. Rp-gt is expressed in ovaries and maternally supplied in the early embryo. The maternal contribution forms a gradient of scattered patches of mRNA in the preblastoderm embryo. Zygotic Rp-gt is expressed in two domains that after germ band extension are restricted to the head and the posterior growth zone. Parental RNAi shows that Rp-gt is required for proper head and abdomen formation. The head lacks mandibulary and maxillary appendages and shows reduced clypeus-labrum, while the abdomen lacks anterior segments. We conclude that Rp-gt is a gap gene on the head and abdomen and, in addition, has a function in patterning the anterior head capsule suggesting that the function of gt in hemipterans is more similar to dipterans than expected. © 2011.Fil: Lavore, Andres Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Centro Regional de Estudios Genómicos; ArgentinaFil: Pagola, Lucia Elena. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Centro Regional de Estudios Genómicos; ArgentinaFil: Esponda Behrens, Natalia Irene. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Centro Regional de Estudios Genómicos; ArgentinaFil: Rivera Pomar, Rolando. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Centro Regional de Estudios Genómicos; Argentina. Universidad Nacional del Noroeste de la Provincia de Buenos Aires. Departamento de Ciencias Básicas y Experimentales; ArgentinaAcademic Press Inc Elsevier Science2012-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/76027Lavore, Andres Esteban; Pagola, Lucia Elena; Esponda Behrens, Natalia Irene; Rivera Pomar, Rolando; The gap gene giant of Rhodnius prolixus is maternally expressed and required for proper head and abdomen formation; Academic Press Inc Elsevier Science; Developmental Biology; 361; 1; 1-2012; 147-1550012-1606CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0012160611010980info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ydbio.2011.06.038info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:57:52Zoai:ri.conicet.gov.ar:11336/76027instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:57:53.687CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv The gap gene giant of Rhodnius prolixus is maternally expressed and required for proper head and abdomen formation
title The gap gene giant of Rhodnius prolixus is maternally expressed and required for proper head and abdomen formation
spellingShingle The gap gene giant of Rhodnius prolixus is maternally expressed and required for proper head and abdomen formation
Lavore, Andres Esteban
Gap Gene
Giant
Maternal Expression
Regulatory Cluster
Rhodnius
Segmentation
title_short The gap gene giant of Rhodnius prolixus is maternally expressed and required for proper head and abdomen formation
title_full The gap gene giant of Rhodnius prolixus is maternally expressed and required for proper head and abdomen formation
title_fullStr The gap gene giant of Rhodnius prolixus is maternally expressed and required for proper head and abdomen formation
title_full_unstemmed The gap gene giant of Rhodnius prolixus is maternally expressed and required for proper head and abdomen formation
title_sort The gap gene giant of Rhodnius prolixus is maternally expressed and required for proper head and abdomen formation
dc.creator.none.fl_str_mv Lavore, Andres Esteban
Pagola, Lucia Elena
Esponda Behrens, Natalia Irene
Rivera Pomar, Rolando
author Lavore, Andres Esteban
author_facet Lavore, Andres Esteban
Pagola, Lucia Elena
Esponda Behrens, Natalia Irene
Rivera Pomar, Rolando
author_role author
author2 Pagola, Lucia Elena
Esponda Behrens, Natalia Irene
Rivera Pomar, Rolando
author2_role author
author
author
dc.subject.none.fl_str_mv Gap Gene
Giant
Maternal Expression
Regulatory Cluster
Rhodnius
Segmentation
topic Gap Gene
Giant
Maternal Expression
Regulatory Cluster
Rhodnius
Segmentation
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The segmentation process in insects depends on a hierarchical cascade of gene activity. The first effectors downstream of the maternal activation are the gap genes, which divide the embryo in broad fields. We discovered a sequence corresponding to the leucine-zipper domain of the orthologue of the gene giant (Rp- gt) in traces from the genome of Rhodnius prolixus, a hemipteran with intermediate germ-band development. We cloned the Rp- gt gene from a normalized cDNA library and characterized its expression and function. Bioinformatic analysis of 12.5. kbp of genomic sequence containing the Rp- gt transcriptional unit shows a cluster of bona fide regulatory binding sites, which is similar in location and structure to the predicted posterior expression domain of the Drosophila orthologue. Rp-gt is expressed in ovaries and maternally supplied in the early embryo. The maternal contribution forms a gradient of scattered patches of mRNA in the preblastoderm embryo. Zygotic Rp-gt is expressed in two domains that after germ band extension are restricted to the head and the posterior growth zone. Parental RNAi shows that Rp-gt is required for proper head and abdomen formation. The head lacks mandibulary and maxillary appendages and shows reduced clypeus-labrum, while the abdomen lacks anterior segments. We conclude that Rp-gt is a gap gene on the head and abdomen and, in addition, has a function in patterning the anterior head capsule suggesting that the function of gt in hemipterans is more similar to dipterans than expected. © 2011.
Fil: Lavore, Andres Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Centro Regional de Estudios Genómicos; Argentina
Fil: Pagola, Lucia Elena. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Centro Regional de Estudios Genómicos; Argentina
Fil: Esponda Behrens, Natalia Irene. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Centro Regional de Estudios Genómicos; Argentina
Fil: Rivera Pomar, Rolando. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Centro Regional de Estudios Genómicos; Argentina. Universidad Nacional del Noroeste de la Provincia de Buenos Aires. Departamento de Ciencias Básicas y Experimentales; Argentina
description The segmentation process in insects depends on a hierarchical cascade of gene activity. The first effectors downstream of the maternal activation are the gap genes, which divide the embryo in broad fields. We discovered a sequence corresponding to the leucine-zipper domain of the orthologue of the gene giant (Rp- gt) in traces from the genome of Rhodnius prolixus, a hemipteran with intermediate germ-band development. We cloned the Rp- gt gene from a normalized cDNA library and characterized its expression and function. Bioinformatic analysis of 12.5. kbp of genomic sequence containing the Rp- gt transcriptional unit shows a cluster of bona fide regulatory binding sites, which is similar in location and structure to the predicted posterior expression domain of the Drosophila orthologue. Rp-gt is expressed in ovaries and maternally supplied in the early embryo. The maternal contribution forms a gradient of scattered patches of mRNA in the preblastoderm embryo. Zygotic Rp-gt is expressed in two domains that after germ band extension are restricted to the head and the posterior growth zone. Parental RNAi shows that Rp-gt is required for proper head and abdomen formation. The head lacks mandibulary and maxillary appendages and shows reduced clypeus-labrum, while the abdomen lacks anterior segments. We conclude that Rp-gt is a gap gene on the head and abdomen and, in addition, has a function in patterning the anterior head capsule suggesting that the function of gt in hemipterans is more similar to dipterans than expected. © 2011.
publishDate 2012
dc.date.none.fl_str_mv 2012-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/76027
Lavore, Andres Esteban; Pagola, Lucia Elena; Esponda Behrens, Natalia Irene; Rivera Pomar, Rolando; The gap gene giant of Rhodnius prolixus is maternally expressed and required for proper head and abdomen formation; Academic Press Inc Elsevier Science; Developmental Biology; 361; 1; 1-2012; 147-155
0012-1606
CONICET Digital
CONICET
url http://hdl.handle.net/11336/76027
identifier_str_mv Lavore, Andres Esteban; Pagola, Lucia Elena; Esponda Behrens, Natalia Irene; Rivera Pomar, Rolando; The gap gene giant of Rhodnius prolixus is maternally expressed and required for proper head and abdomen formation; Academic Press Inc Elsevier Science; Developmental Biology; 361; 1; 1-2012; 147-155
0012-1606
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0012160611010980
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ydbio.2011.06.038
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Academic Press Inc Elsevier Science
publisher.none.fl_str_mv Academic Press Inc Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269489378361344
score 13.13397

Publicaciones similares