Vitamin E prevents ethylene bis(dithiocarbamate) pesticide zineb-induced sister chromatid exchange in Chinese hamster ovary cells
- Autores
- Soloneski, Sonia María Elsa; Reigosa, Miguel A.; Larramendy, Marcelo Luis
- Año de publicación
- 2003
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The in vitro effect of the antioxidant α-tocopherol, vitamin E, on deleterious effects induced by the dithiocarbamate fungicide zineb and its commercial formulation azzurro on Chinese hamster ovary (CHO) cells was studied by using frequency of sister chromatid exchanges (SCEs), cell cycle progression and mitotic index (MI) as genetic end points. Both zineb and azzurro activities were tested within the range 0.1-100.0 μg/ml on exponentially growing CHO cells preincubated for 24 h in the presence or absence of 50.0 μg/ml vitamin E. SCE frequencies increased significantly over control values in a concentration-dependent manner in zineb- and azzurro-treated cultures at concentrations of 0.1-10.0 and 0.1-25.0 μg/ml, respectively. When target cells were preincubated with vitamin E, the number of SCEs was significantly lower than that observed in cells exposed only to 1.0-10.0 μg/ml zineb or 1.0-25.0 μg/ml azzurro, but higher than control values. Cytotoxicity was observed at concentrations higher than 25.0 and 50.0 μg/ml zineb and azzurro, respectively, regardless of the absence or presence of vitamin E. Regression analysis showed that the proliferative rate index decreased as a function of the concentration of zineb (0.1-10.0 μg/ml concentration range) and azzurro (0.1-25.0 μg/ml concentration range) titrated into cultures. For both chemicals, progressive concentration-related inhibition of the mitotic activity from cultures was observed when 10.0 μg/ml zineb or 1.0-25.0 μg/ml azzurro was employed. However, no significant alteration in cell cycle progression or MI was observed between vitamin E-preincubated cultures and those treated only with zineb and azzurro.
Facultad de Ciencias Naturales y Museo - Materia
-
Ciencias Naturales
Vitamina E - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by-nc-sa/4.0/
- Repositorio
- Institución
- Universidad Nacional de La Plata
- OAI Identificador
- oai:sedici.unlp.edu.ar:10915/84783
Ver los metadatos del registro completo
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Vitamin E prevents ethylene bis(dithiocarbamate) pesticide zineb-induced sister chromatid exchange in Chinese hamster ovary cellsSoloneski, Sonia María ElsaReigosa, Miguel A.Larramendy, Marcelo LuisCiencias NaturalesVitamina EThe in vitro effect of the antioxidant α-tocopherol, vitamin E, on deleterious effects induced by the dithiocarbamate fungicide zineb and its commercial formulation azzurro on Chinese hamster ovary (CHO) cells was studied by using frequency of sister chromatid exchanges (SCEs), cell cycle progression and mitotic index (MI) as genetic end points. Both zineb and azzurro activities were tested within the range 0.1-100.0 μg/ml on exponentially growing CHO cells preincubated for 24 h in the presence or absence of 50.0 μg/ml vitamin E. SCE frequencies increased significantly over control values in a concentration-dependent manner in zineb- and azzurro-treated cultures at concentrations of 0.1-10.0 and 0.1-25.0 μg/ml, respectively. When target cells were preincubated with vitamin E, the number of SCEs was significantly lower than that observed in cells exposed only to 1.0-10.0 μg/ml zineb or 1.0-25.0 μg/ml azzurro, but higher than control values. Cytotoxicity was observed at concentrations higher than 25.0 and 50.0 μg/ml zineb and azzurro, respectively, regardless of the absence or presence of vitamin E. Regression analysis showed that the proliferative rate index decreased as a function of the concentration of zineb (0.1-10.0 μg/ml concentration range) and azzurro (0.1-25.0 μg/ml concentration range) titrated into cultures. For both chemicals, progressive concentration-related inhibition of the mitotic activity from cultures was observed when 10.0 μg/ml zineb or 1.0-25.0 μg/ml azzurro was employed. However, no significant alteration in cell cycle progression or MI was observed between vitamin E-preincubated cultures and those treated only with zineb and azzurro.Facultad de Ciencias Naturales y Museo2003info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf505-510http://sedici.unlp.edu.ar/handle/10915/84783enginfo:eu-repo/semantics/altIdentifier/issn/0267-8357info:eu-repo/semantics/altIdentifier/doi/10.1093/mutage/geg026info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:16:15Zoai:sedici.unlp.edu.ar:10915/84783Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:16:16.066SEDICI (UNLP) - Universidad Nacional de La Platafalse |
dc.title.none.fl_str_mv |
Vitamin E prevents ethylene bis(dithiocarbamate) pesticide zineb-induced sister chromatid exchange in Chinese hamster ovary cells |
title |
Vitamin E prevents ethylene bis(dithiocarbamate) pesticide zineb-induced sister chromatid exchange in Chinese hamster ovary cells |
spellingShingle |
Vitamin E prevents ethylene bis(dithiocarbamate) pesticide zineb-induced sister chromatid exchange in Chinese hamster ovary cells Soloneski, Sonia María Elsa Ciencias Naturales Vitamina E |
title_short |
Vitamin E prevents ethylene bis(dithiocarbamate) pesticide zineb-induced sister chromatid exchange in Chinese hamster ovary cells |
title_full |
Vitamin E prevents ethylene bis(dithiocarbamate) pesticide zineb-induced sister chromatid exchange in Chinese hamster ovary cells |
title_fullStr |
Vitamin E prevents ethylene bis(dithiocarbamate) pesticide zineb-induced sister chromatid exchange in Chinese hamster ovary cells |
title_full_unstemmed |
Vitamin E prevents ethylene bis(dithiocarbamate) pesticide zineb-induced sister chromatid exchange in Chinese hamster ovary cells |
title_sort |
Vitamin E prevents ethylene bis(dithiocarbamate) pesticide zineb-induced sister chromatid exchange in Chinese hamster ovary cells |
dc.creator.none.fl_str_mv |
Soloneski, Sonia María Elsa Reigosa, Miguel A. Larramendy, Marcelo Luis |
author |
Soloneski, Sonia María Elsa |
author_facet |
Soloneski, Sonia María Elsa Reigosa, Miguel A. Larramendy, Marcelo Luis |
author_role |
author |
author2 |
Reigosa, Miguel A. Larramendy, Marcelo Luis |
author2_role |
author author |
dc.subject.none.fl_str_mv |
Ciencias Naturales Vitamina E |
topic |
Ciencias Naturales Vitamina E |
dc.description.none.fl_txt_mv |
The in vitro effect of the antioxidant α-tocopherol, vitamin E, on deleterious effects induced by the dithiocarbamate fungicide zineb and its commercial formulation azzurro on Chinese hamster ovary (CHO) cells was studied by using frequency of sister chromatid exchanges (SCEs), cell cycle progression and mitotic index (MI) as genetic end points. Both zineb and azzurro activities were tested within the range 0.1-100.0 μg/ml on exponentially growing CHO cells preincubated for 24 h in the presence or absence of 50.0 μg/ml vitamin E. SCE frequencies increased significantly over control values in a concentration-dependent manner in zineb- and azzurro-treated cultures at concentrations of 0.1-10.0 and 0.1-25.0 μg/ml, respectively. When target cells were preincubated with vitamin E, the number of SCEs was significantly lower than that observed in cells exposed only to 1.0-10.0 μg/ml zineb or 1.0-25.0 μg/ml azzurro, but higher than control values. Cytotoxicity was observed at concentrations higher than 25.0 and 50.0 μg/ml zineb and azzurro, respectively, regardless of the absence or presence of vitamin E. Regression analysis showed that the proliferative rate index decreased as a function of the concentration of zineb (0.1-10.0 μg/ml concentration range) and azzurro (0.1-25.0 μg/ml concentration range) titrated into cultures. For both chemicals, progressive concentration-related inhibition of the mitotic activity from cultures was observed when 10.0 μg/ml zineb or 1.0-25.0 μg/ml azzurro was employed. However, no significant alteration in cell cycle progression or MI was observed between vitamin E-preincubated cultures and those treated only with zineb and azzurro. Facultad de Ciencias Naturales y Museo |
description |
The in vitro effect of the antioxidant α-tocopherol, vitamin E, on deleterious effects induced by the dithiocarbamate fungicide zineb and its commercial formulation azzurro on Chinese hamster ovary (CHO) cells was studied by using frequency of sister chromatid exchanges (SCEs), cell cycle progression and mitotic index (MI) as genetic end points. Both zineb and azzurro activities were tested within the range 0.1-100.0 μg/ml on exponentially growing CHO cells preincubated for 24 h in the presence or absence of 50.0 μg/ml vitamin E. SCE frequencies increased significantly over control values in a concentration-dependent manner in zineb- and azzurro-treated cultures at concentrations of 0.1-10.0 and 0.1-25.0 μg/ml, respectively. When target cells were preincubated with vitamin E, the number of SCEs was significantly lower than that observed in cells exposed only to 1.0-10.0 μg/ml zineb or 1.0-25.0 μg/ml azzurro, but higher than control values. Cytotoxicity was observed at concentrations higher than 25.0 and 50.0 μg/ml zineb and azzurro, respectively, regardless of the absence or presence of vitamin E. Regression analysis showed that the proliferative rate index decreased as a function of the concentration of zineb (0.1-10.0 μg/ml concentration range) and azzurro (0.1-25.0 μg/ml concentration range) titrated into cultures. For both chemicals, progressive concentration-related inhibition of the mitotic activity from cultures was observed when 10.0 μg/ml zineb or 1.0-25.0 μg/ml azzurro was employed. However, no significant alteration in cell cycle progression or MI was observed between vitamin E-preincubated cultures and those treated only with zineb and azzurro. |
publishDate |
2003 |
dc.date.none.fl_str_mv |
2003 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Articulo http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
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article |
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publishedVersion |
dc.identifier.none.fl_str_mv |
http://sedici.unlp.edu.ar/handle/10915/84783 |
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http://sedici.unlp.edu.ar/handle/10915/84783 |
dc.language.none.fl_str_mv |
eng |
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eng |
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openAccess |
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http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
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