DNA damage kinetics and apoptosis in ivermectin-treated chinese hamster ovary cells

Autores
Molinari, Gabriela Beatriz; Kujawski, Maciej; Scuto, Anna; Soloneski, Sonia Maria Elsa; Larramendy, Marcelo Luis
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A comet assay was used to analyze DNA damage kinetics in Chinese hamster ovary (CHO-K1) cells induced by antiparasitic ivermectin (IVM) and the IVM-containing technical formulation IvomecW (IVO; 1% IVM). Cells were treated with 50 mg ml–1 IVM and IVO for 80 min, washed and re-incubated in antiparasiticide-free medium for 0–24 h until assayed using the single-cell gel electrophoresis assay (SCGE). Cell viability remained unchanged up to 3 h of incubation. After 6 h of treatment, cell survival decreased up to 75% and 79% in IVM- and IVO-treated cultures, respectively, remaining unchanged within 12–24 h after treatment. For both anthelmintics, biphasic behavior in DNA damage occurred during the incubation time. A time-dependent increase of IVM- and IVO-induced DNA damage was observed within 0 to 3 h after pulse treatment, revealed by a progressive decrease of undamaged cells and an increase in slightly damaged and damaged cells. Finally, a time-dependent decrease in IVM- and IVO-induced DNA damage was revealed by a progressive decrease of slightly damaged cells and the absence of damaged cells simultaneously with an increase in the frequency of undamaged cells during the final 18 h of incubation. Flow cytometry analysis revealed that both compounds are able to induce a marked increase in early and late apoptosis. Based on our observations, we could conclude that the decrease in DNA lesions is mostly related to IVMinduced cytotoxicity rather than attributable to a repair process. Copyright © 2012 John Wiley & Sons, Ltd.
Fil: Molinari, Gabriela Beatriz. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Kujawski, Maciej. City of Hope Comprehensive Cancer Center; Estados Unidos
Fil: Scuto, Anna. City of Hope Comprehensive Cancer Center; Estados Unidos
Fil: Soloneski, Sonia Maria Elsa. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Larramendy, Marcelo Luis. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
Cho-K1 Cells
Comet Assay
Flow Cytometry
Apoptosis
Ivermectin
Ivomecw
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/21940
Acceder
id CONICETDig_540562d1b9887258997c3c93ee4389ab
oai_identifier_str oai:ri.conicet.gov.ar:11336/21940
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling DNA damage kinetics and apoptosis in ivermectin-treated chinese hamster ovary cellsMolinari, Gabriela BeatrizKujawski, MaciejScuto, AnnaSoloneski, Sonia Maria ElsaLarramendy, Marcelo LuisCho-K1 CellsComet AssayFlow CytometryApoptosisIvermectinIvomecwhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1A comet assay was used to analyze DNA damage kinetics in Chinese hamster ovary (CHO-K1) cells induced by antiparasitic ivermectin (IVM) and the IVM-containing technical formulation IvomecW (IVO; 1% IVM). Cells were treated with 50 mg ml–1 IVM and IVO for 80 min, washed and re-incubated in antiparasiticide-free medium for 0–24 h until assayed using the single-cell gel electrophoresis assay (SCGE). Cell viability remained unchanged up to 3 h of incubation. After 6 h of treatment, cell survival decreased up to 75% and 79% in IVM- and IVO-treated cultures, respectively, remaining unchanged within 12–24 h after treatment. For both anthelmintics, biphasic behavior in DNA damage occurred during the incubation time. A time-dependent increase of IVM- and IVO-induced DNA damage was observed within 0 to 3 h after pulse treatment, revealed by a progressive decrease of undamaged cells and an increase in slightly damaged and damaged cells. Finally, a time-dependent decrease in IVM- and IVO-induced DNA damage was revealed by a progressive decrease of slightly damaged cells and the absence of damaged cells simultaneously with an increase in the frequency of undamaged cells during the final 18 h of incubation. Flow cytometry analysis revealed that both compounds are able to induce a marked increase in early and late apoptosis. Based on our observations, we could conclude that the decrease in DNA lesions is mostly related to IVMinduced cytotoxicity rather than attributable to a repair process. Copyright © 2012 John Wiley & Sons, Ltd.Fil: Molinari, Gabriela Beatriz. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Kujawski, Maciej. City of Hope Comprehensive Cancer Center; Estados UnidosFil: Scuto, Anna. City of Hope Comprehensive Cancer Center; Estados UnidosFil: Soloneski, Sonia Maria Elsa. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Larramendy, Marcelo Luis. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaWiley2012-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/21940Molinari, Gabriela Beatriz; Kujawski, Maciej; Scuto, Anna; Soloneski, Sonia Maria Elsa; Larramendy, Marcelo Luis; DNA damage kinetics and apoptosis in ivermectin-treated chinese hamster ovary cells; Wiley; Journal of Applied Toxicology; 33; 11; 9-2012; 1260-12670260-437XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1002/jat.2782info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/jat.2782/abstractinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:07:07Zoai:ri.conicet.gov.ar:11336/21940instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:07:07.419CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv DNA damage kinetics and apoptosis in ivermectin-treated chinese hamster ovary cells
title DNA damage kinetics and apoptosis in ivermectin-treated chinese hamster ovary cells
spellingShingle DNA damage kinetics and apoptosis in ivermectin-treated chinese hamster ovary cells
Molinari, Gabriela Beatriz
Cho-K1 Cells
Comet Assay
Flow Cytometry
Apoptosis
Ivermectin
Ivomecw
title_short DNA damage kinetics and apoptosis in ivermectin-treated chinese hamster ovary cells
title_full DNA damage kinetics and apoptosis in ivermectin-treated chinese hamster ovary cells
title_fullStr DNA damage kinetics and apoptosis in ivermectin-treated chinese hamster ovary cells
title_full_unstemmed DNA damage kinetics and apoptosis in ivermectin-treated chinese hamster ovary cells
title_sort DNA damage kinetics and apoptosis in ivermectin-treated chinese hamster ovary cells
dc.creator.none.fl_str_mv Molinari, Gabriela Beatriz
Kujawski, Maciej
Scuto, Anna
Soloneski, Sonia Maria Elsa
Larramendy, Marcelo Luis
author Molinari, Gabriela Beatriz
author_facet Molinari, Gabriela Beatriz
Kujawski, Maciej
Scuto, Anna
Soloneski, Sonia Maria Elsa
Larramendy, Marcelo Luis
author_role author
author2 Kujawski, Maciej
Scuto, Anna
Soloneski, Sonia Maria Elsa
Larramendy, Marcelo Luis
author2_role author
author
author
author
dc.subject.none.fl_str_mv Cho-K1 Cells
Comet Assay
Flow Cytometry
Apoptosis
Ivermectin
Ivomecw
topic Cho-K1 Cells
Comet Assay
Flow Cytometry
Apoptosis
Ivermectin
Ivomecw
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv A comet assay was used to analyze DNA damage kinetics in Chinese hamster ovary (CHO-K1) cells induced by antiparasitic ivermectin (IVM) and the IVM-containing technical formulation IvomecW (IVO; 1% IVM). Cells were treated with 50 mg ml–1 IVM and IVO for 80 min, washed and re-incubated in antiparasiticide-free medium for 0–24 h until assayed using the single-cell gel electrophoresis assay (SCGE). Cell viability remained unchanged up to 3 h of incubation. After 6 h of treatment, cell survival decreased up to 75% and 79% in IVM- and IVO-treated cultures, respectively, remaining unchanged within 12–24 h after treatment. For both anthelmintics, biphasic behavior in DNA damage occurred during the incubation time. A time-dependent increase of IVM- and IVO-induced DNA damage was observed within 0 to 3 h after pulse treatment, revealed by a progressive decrease of undamaged cells and an increase in slightly damaged and damaged cells. Finally, a time-dependent decrease in IVM- and IVO-induced DNA damage was revealed by a progressive decrease of slightly damaged cells and the absence of damaged cells simultaneously with an increase in the frequency of undamaged cells during the final 18 h of incubation. Flow cytometry analysis revealed that both compounds are able to induce a marked increase in early and late apoptosis. Based on our observations, we could conclude that the decrease in DNA lesions is mostly related to IVMinduced cytotoxicity rather than attributable to a repair process. Copyright © 2012 John Wiley & Sons, Ltd.
Fil: Molinari, Gabriela Beatriz. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Kujawski, Maciej. City of Hope Comprehensive Cancer Center; Estados Unidos
Fil: Scuto, Anna. City of Hope Comprehensive Cancer Center; Estados Unidos
Fil: Soloneski, Sonia Maria Elsa. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Larramendy, Marcelo Luis. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description A comet assay was used to analyze DNA damage kinetics in Chinese hamster ovary (CHO-K1) cells induced by antiparasitic ivermectin (IVM) and the IVM-containing technical formulation IvomecW (IVO; 1% IVM). Cells were treated with 50 mg ml–1 IVM and IVO for 80 min, washed and re-incubated in antiparasiticide-free medium for 0–24 h until assayed using the single-cell gel electrophoresis assay (SCGE). Cell viability remained unchanged up to 3 h of incubation. After 6 h of treatment, cell survival decreased up to 75% and 79% in IVM- and IVO-treated cultures, respectively, remaining unchanged within 12–24 h after treatment. For both anthelmintics, biphasic behavior in DNA damage occurred during the incubation time. A time-dependent increase of IVM- and IVO-induced DNA damage was observed within 0 to 3 h after pulse treatment, revealed by a progressive decrease of undamaged cells and an increase in slightly damaged and damaged cells. Finally, a time-dependent decrease in IVM- and IVO-induced DNA damage was revealed by a progressive decrease of slightly damaged cells and the absence of damaged cells simultaneously with an increase in the frequency of undamaged cells during the final 18 h of incubation. Flow cytometry analysis revealed that both compounds are able to induce a marked increase in early and late apoptosis. Based on our observations, we could conclude that the decrease in DNA lesions is mostly related to IVMinduced cytotoxicity rather than attributable to a repair process. Copyright © 2012 John Wiley & Sons, Ltd.
publishDate 2012
dc.date.none.fl_str_mv 2012-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/21940
Molinari, Gabriela Beatriz; Kujawski, Maciej; Scuto, Anna; Soloneski, Sonia Maria Elsa; Larramendy, Marcelo Luis; DNA damage kinetics and apoptosis in ivermectin-treated chinese hamster ovary cells; Wiley; Journal of Applied Toxicology; 33; 11; 9-2012; 1260-1267
0260-437X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/21940
identifier_str_mv Molinari, Gabriela Beatriz; Kujawski, Maciej; Scuto, Anna; Soloneski, Sonia Maria Elsa; Larramendy, Marcelo Luis; DNA damage kinetics and apoptosis in ivermectin-treated chinese hamster ovary cells; Wiley; Journal of Applied Toxicology; 33; 11; 9-2012; 1260-1267
0260-437X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1002/jat.2782
info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/jat.2782/abstract
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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