Sister chromatid exchanges and chromosomal aberrations in Chinese Hamster Ovary (CHO-K1) cells treated with insecticide pirimicarb

Autores
Soloneski, Sonia Maria Elsa; Larramendy, Marcelo Luis
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Pirimicarb and its formulation Aficida® (50% pirimicarb) effects were studied on CHO-K1 cells employing sister chromatid exchange (SCE), chromosomal aberrations (CA), cell-cycle progression and mitotic index analyses. Continuous treatments were performed within 10-300 μg/ml concentration-range. Pirimicarb, but not Aficida®, induced a concentration-dependent increase of abnormal cells. Pirimicarb induced a greater frequency of chromatid/isochromatid breaks than Aficida® did. Regression analyses showed a concentration-dependent increase in the frequency of chromatid-type breaks for both compounds whereas only the frequency of isochromatid-type breaks did in those pirimicarb-treated cultures. SCEs in pirimicarb- or Aficida®-treated cultures were significantly higher than control values with concentrations of 100-200 μg/ml. Both test compounds induced equivalent frequency of SCEs. A delay in cell-cycle kinetics was observed for pirimicarb and Aficida® within 100-300 and 200-300 μg/ml concentration-range, respectively. An inhibition of MI was observed for both chemicals regardless of tested concentrations. Finally, the CAs appears to be a higher sensitive bioassay to detect DNA damage at lower concentrations of pirimicarb than SCEs does. The results demonstrated that pirimicarb and Aficida® exert geno-cytotoxicity, at least in CHO-K1 cells.
Fil: Soloneski, Sonia Maria Elsa. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Laboratorio de Citogenética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Larramendy, Marcelo Luis. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Laboratorio de Citogenética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Materia
CARBAMATES
CYTOTOXICITY
GENOTOXICITY
MAMMALIAN CELL LINES
TECHNICAL FORMULATIONS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/193027
Acceder
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network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling Sister chromatid exchanges and chromosomal aberrations in Chinese Hamster Ovary (CHO-K1) cells treated with insecticide pirimicarbSoloneski, Sonia Maria ElsaLarramendy, Marcelo LuisCARBAMATESCYTOTOXICITYGENOTOXICITYMAMMALIAN CELL LINESTECHNICAL FORMULATIONShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Pirimicarb and its formulation Aficida® (50% pirimicarb) effects were studied on CHO-K1 cells employing sister chromatid exchange (SCE), chromosomal aberrations (CA), cell-cycle progression and mitotic index analyses. Continuous treatments were performed within 10-300 μg/ml concentration-range. Pirimicarb, but not Aficida®, induced a concentration-dependent increase of abnormal cells. Pirimicarb induced a greater frequency of chromatid/isochromatid breaks than Aficida® did. Regression analyses showed a concentration-dependent increase in the frequency of chromatid-type breaks for both compounds whereas only the frequency of isochromatid-type breaks did in those pirimicarb-treated cultures. SCEs in pirimicarb- or Aficida®-treated cultures were significantly higher than control values with concentrations of 100-200 μg/ml. Both test compounds induced equivalent frequency of SCEs. A delay in cell-cycle kinetics was observed for pirimicarb and Aficida® within 100-300 and 200-300 μg/ml concentration-range, respectively. An inhibition of MI was observed for both chemicals regardless of tested concentrations. Finally, the CAs appears to be a higher sensitive bioassay to detect DNA damage at lower concentrations of pirimicarb than SCEs does. The results demonstrated that pirimicarb and Aficida® exert geno-cytotoxicity, at least in CHO-K1 cells.Fil: Soloneski, Sonia Maria Elsa. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Laboratorio de Citogenética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaFil: Larramendy, Marcelo Luis. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Laboratorio de Citogenética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaElsevier Science2010-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/193027Soloneski, Sonia Maria Elsa; Larramendy, Marcelo Luis; Sister chromatid exchanges and chromosomal aberrations in Chinese Hamster Ovary (CHO-K1) cells treated with insecticide pirimicarb; Elsevier Science; Journal of Hazardous Materials; 174; 1-3; 2-2010; 410-4150304-3894CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0304389409015295info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jhazmat.2009.09.068info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:22:58Zoai:ri.conicet.gov.ar:11336/193027instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:22:58.285CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Sister chromatid exchanges and chromosomal aberrations in Chinese Hamster Ovary (CHO-K1) cells treated with insecticide pirimicarb
title Sister chromatid exchanges and chromosomal aberrations in Chinese Hamster Ovary (CHO-K1) cells treated with insecticide pirimicarb
spellingShingle Sister chromatid exchanges and chromosomal aberrations in Chinese Hamster Ovary (CHO-K1) cells treated with insecticide pirimicarb
Soloneski, Sonia Maria Elsa
CARBAMATES
CYTOTOXICITY
GENOTOXICITY
MAMMALIAN CELL LINES
TECHNICAL FORMULATIONS
title_short Sister chromatid exchanges and chromosomal aberrations in Chinese Hamster Ovary (CHO-K1) cells treated with insecticide pirimicarb
title_full Sister chromatid exchanges and chromosomal aberrations in Chinese Hamster Ovary (CHO-K1) cells treated with insecticide pirimicarb
title_fullStr Sister chromatid exchanges and chromosomal aberrations in Chinese Hamster Ovary (CHO-K1) cells treated with insecticide pirimicarb
title_full_unstemmed Sister chromatid exchanges and chromosomal aberrations in Chinese Hamster Ovary (CHO-K1) cells treated with insecticide pirimicarb
title_sort Sister chromatid exchanges and chromosomal aberrations in Chinese Hamster Ovary (CHO-K1) cells treated with insecticide pirimicarb
dc.creator.none.fl_str_mv Soloneski, Sonia Maria Elsa
Larramendy, Marcelo Luis
author Soloneski, Sonia Maria Elsa
author_facet Soloneski, Sonia Maria Elsa
Larramendy, Marcelo Luis
author_role author
author2 Larramendy, Marcelo Luis
author2_role author
dc.subject.none.fl_str_mv CARBAMATES
CYTOTOXICITY
GENOTOXICITY
MAMMALIAN CELL LINES
TECHNICAL FORMULATIONS
topic CARBAMATES
CYTOTOXICITY
GENOTOXICITY
MAMMALIAN CELL LINES
TECHNICAL FORMULATIONS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Pirimicarb and its formulation Aficida® (50% pirimicarb) effects were studied on CHO-K1 cells employing sister chromatid exchange (SCE), chromosomal aberrations (CA), cell-cycle progression and mitotic index analyses. Continuous treatments were performed within 10-300 μg/ml concentration-range. Pirimicarb, but not Aficida®, induced a concentration-dependent increase of abnormal cells. Pirimicarb induced a greater frequency of chromatid/isochromatid breaks than Aficida® did. Regression analyses showed a concentration-dependent increase in the frequency of chromatid-type breaks for both compounds whereas only the frequency of isochromatid-type breaks did in those pirimicarb-treated cultures. SCEs in pirimicarb- or Aficida®-treated cultures were significantly higher than control values with concentrations of 100-200 μg/ml. Both test compounds induced equivalent frequency of SCEs. A delay in cell-cycle kinetics was observed for pirimicarb and Aficida® within 100-300 and 200-300 μg/ml concentration-range, respectively. An inhibition of MI was observed for both chemicals regardless of tested concentrations. Finally, the CAs appears to be a higher sensitive bioassay to detect DNA damage at lower concentrations of pirimicarb than SCEs does. The results demonstrated that pirimicarb and Aficida® exert geno-cytotoxicity, at least in CHO-K1 cells.
Fil: Soloneski, Sonia Maria Elsa. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Laboratorio de Citogenética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Larramendy, Marcelo Luis. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Laboratorio de Citogenética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
description Pirimicarb and its formulation Aficida® (50% pirimicarb) effects were studied on CHO-K1 cells employing sister chromatid exchange (SCE), chromosomal aberrations (CA), cell-cycle progression and mitotic index analyses. Continuous treatments were performed within 10-300 μg/ml concentration-range. Pirimicarb, but not Aficida®, induced a concentration-dependent increase of abnormal cells. Pirimicarb induced a greater frequency of chromatid/isochromatid breaks than Aficida® did. Regression analyses showed a concentration-dependent increase in the frequency of chromatid-type breaks for both compounds whereas only the frequency of isochromatid-type breaks did in those pirimicarb-treated cultures. SCEs in pirimicarb- or Aficida®-treated cultures were significantly higher than control values with concentrations of 100-200 μg/ml. Both test compounds induced equivalent frequency of SCEs. A delay in cell-cycle kinetics was observed for pirimicarb and Aficida® within 100-300 and 200-300 μg/ml concentration-range, respectively. An inhibition of MI was observed for both chemicals regardless of tested concentrations. Finally, the CAs appears to be a higher sensitive bioassay to detect DNA damage at lower concentrations of pirimicarb than SCEs does. The results demonstrated that pirimicarb and Aficida® exert geno-cytotoxicity, at least in CHO-K1 cells.
publishDate 2010
dc.date.none.fl_str_mv 2010-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/193027
Soloneski, Sonia Maria Elsa; Larramendy, Marcelo Luis; Sister chromatid exchanges and chromosomal aberrations in Chinese Hamster Ovary (CHO-K1) cells treated with insecticide pirimicarb; Elsevier Science; Journal of Hazardous Materials; 174; 1-3; 2-2010; 410-415
0304-3894
CONICET Digital
CONICET
url http://hdl.handle.net/11336/193027
identifier_str_mv Soloneski, Sonia Maria Elsa; Larramendy, Marcelo Luis; Sister chromatid exchanges and chromosomal aberrations in Chinese Hamster Ovary (CHO-K1) cells treated with insecticide pirimicarb; Elsevier Science; Journal of Hazardous Materials; 174; 1-3; 2-2010; 410-415
0304-3894
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0304389409015295
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jhazmat.2009.09.068
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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