Interaction of galacto-oligosaccharides and lactulose with dipalmitoylphosphatidilcholine lipid membranes as determined by infrared spectroscopy

Autores
Díaz, Sonia Beatriz; Ale, Norma Mercedes; Ben Altabef, Aída; Tymczyszyn, Emma Elizabeth; Gómez-Zavaglia, Andrea
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Lactulose and galacto-oligosaccharides (GOS) have been recognized as health promoting compounds due to their recognized prebiotic capacity. In addition, because of their polyhydroxilated nature, they are effective protectants during processes involving dehydration of lactic acid bacteria. The protective effects of sugars have been associated with their capacity to replace water molecules from lipid membranes and/or to form glassy matrices. Although this latter property has been previously investigated for GOS and lactulose, the capacity of these sugars to replace water molecules was not. Furthermore, it must be considered that GOS are usually mixtures of oligo-saccharides with differentdegrees of polymerization (DP) including or excluding different amounts of mono and disaccharides, and the effect of their composition on the GOS-membrane interaction has not been addressed so far. The aim of the present work was to study the interaction of lactulose and GOS of different compositions with dipalmitoylphosphatidilcholine (DPPC) membranes both in the gel (Lb) and in the liquid crystalline phase (La) by using infrared spectroscopy. Both GOS and lactulose interacted with the phosphate groups of the polar head region, but only lactulose (disaccharide) penetrated into the lipid bilayers anddecreased the membrane phase transition (Tm). In contrast, GOS containing no mono and disaccharides were excluded from the hydrophobic region, leading to an increase of the Tm. In turn, GOS containing mono and disaccharides showed a concentration-dependent effect. The results obtained shed light on the mechanisms involved in protection mediated by GOS and lactulose, thus providing support to improve the stabilization of lactic acid bacteria.
Centro de Investigación y Desarrollo en Criotecnología de Alimentos
Materia
Bioquímica
GOS
Lactolose
DPPC
Membrane
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/99825

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network_name_str SEDICI (UNLP)
spelling Interaction of galacto-oligosaccharides and lactulose with dipalmitoylphosphatidilcholine lipid membranes as determined by infrared spectroscopyDíaz, Sonia BeatrizAle, Norma MercedesBen Altabef, AídaTymczyszyn, Emma ElizabethGómez-Zavaglia, AndreaBioquímicaGOSLactoloseDPPCMembraneLactulose and galacto-oligosaccharides (GOS) have been recognized as health promoting compounds due to their recognized prebiotic capacity. In addition, because of their polyhydroxilated nature, they are effective protectants during processes involving dehydration of lactic acid bacteria. The protective effects of sugars have been associated with their capacity to replace water molecules from lipid membranes and/or to form glassy matrices. Although this latter property has been previously investigated for GOS and lactulose, the capacity of these sugars to replace water molecules was not. Furthermore, it must be considered that GOS are usually mixtures of oligo-saccharides with differentdegrees of polymerization (DP) including or excluding different amounts of mono and disaccharides, and the effect of their composition on the GOS-membrane interaction has not been addressed so far. The aim of the present work was to study the interaction of lactulose and GOS of different compositions with dipalmitoylphosphatidilcholine (DPPC) membranes both in the gel (Lb) and in the liquid crystalline phase (La) by using infrared spectroscopy. Both GOS and lactulose interacted with the phosphate groups of the polar head region, but only lactulose (disaccharide) penetrated into the lipid bilayers anddecreased the membrane phase transition (Tm). In contrast, GOS containing no mono and disaccharides were excluded from the hydrophobic region, leading to an increase of the Tm. In turn, GOS containing mono and disaccharides showed a concentration-dependent effect. The results obtained shed light on the mechanisms involved in protection mediated by GOS and lactulose, thus providing support to improve the stabilization of lactic acid bacteria.Centro de Investigación y Desarrollo en Criotecnología de Alimentos2017-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf24298-24304http://sedici.unlp.edu.ar/handle/10915/99825enginfo:eu-repo/semantics/altIdentifier/url/https://ri.conicet.gov.ar/11336/40842info:eu-repo/semantics/altIdentifier/url/http://pubs.rsc.org/en/Content/ArticleLanding/2017/RA/C7RA01964Einfo:eu-repo/semantics/altIdentifier/issn/2046-2069info:eu-repo/semantics/altIdentifier/doi/10.1039/C7RA01964Einfo:eu-repo/semantics/altIdentifier/hdl/11336/40842info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc/4.0/Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:20:05Zoai:sedici.unlp.edu.ar:10915/99825Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:20:05.942SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Interaction of galacto-oligosaccharides and lactulose with dipalmitoylphosphatidilcholine lipid membranes as determined by infrared spectroscopy
title Interaction of galacto-oligosaccharides and lactulose with dipalmitoylphosphatidilcholine lipid membranes as determined by infrared spectroscopy
spellingShingle Interaction of galacto-oligosaccharides and lactulose with dipalmitoylphosphatidilcholine lipid membranes as determined by infrared spectroscopy
Díaz, Sonia Beatriz
Bioquímica
GOS
Lactolose
DPPC
Membrane
title_short Interaction of galacto-oligosaccharides and lactulose with dipalmitoylphosphatidilcholine lipid membranes as determined by infrared spectroscopy
title_full Interaction of galacto-oligosaccharides and lactulose with dipalmitoylphosphatidilcholine lipid membranes as determined by infrared spectroscopy
title_fullStr Interaction of galacto-oligosaccharides and lactulose with dipalmitoylphosphatidilcholine lipid membranes as determined by infrared spectroscopy
title_full_unstemmed Interaction of galacto-oligosaccharides and lactulose with dipalmitoylphosphatidilcholine lipid membranes as determined by infrared spectroscopy
title_sort Interaction of galacto-oligosaccharides and lactulose with dipalmitoylphosphatidilcholine lipid membranes as determined by infrared spectroscopy
dc.creator.none.fl_str_mv Díaz, Sonia Beatriz
Ale, Norma Mercedes
Ben Altabef, Aída
Tymczyszyn, Emma Elizabeth
Gómez-Zavaglia, Andrea
author Díaz, Sonia Beatriz
author_facet Díaz, Sonia Beatriz
Ale, Norma Mercedes
Ben Altabef, Aída
Tymczyszyn, Emma Elizabeth
Gómez-Zavaglia, Andrea
author_role author
author2 Ale, Norma Mercedes
Ben Altabef, Aída
Tymczyszyn, Emma Elizabeth
Gómez-Zavaglia, Andrea
author2_role author
author
author
author
dc.subject.none.fl_str_mv Bioquímica
GOS
Lactolose
DPPC
Membrane
topic Bioquímica
GOS
Lactolose
DPPC
Membrane
dc.description.none.fl_txt_mv Lactulose and galacto-oligosaccharides (GOS) have been recognized as health promoting compounds due to their recognized prebiotic capacity. In addition, because of their polyhydroxilated nature, they are effective protectants during processes involving dehydration of lactic acid bacteria. The protective effects of sugars have been associated with their capacity to replace water molecules from lipid membranes and/or to form glassy matrices. Although this latter property has been previously investigated for GOS and lactulose, the capacity of these sugars to replace water molecules was not. Furthermore, it must be considered that GOS are usually mixtures of oligo-saccharides with differentdegrees of polymerization (DP) including or excluding different amounts of mono and disaccharides, and the effect of their composition on the GOS-membrane interaction has not been addressed so far. The aim of the present work was to study the interaction of lactulose and GOS of different compositions with dipalmitoylphosphatidilcholine (DPPC) membranes both in the gel (Lb) and in the liquid crystalline phase (La) by using infrared spectroscopy. Both GOS and lactulose interacted with the phosphate groups of the polar head region, but only lactulose (disaccharide) penetrated into the lipid bilayers anddecreased the membrane phase transition (Tm). In contrast, GOS containing no mono and disaccharides were excluded from the hydrophobic region, leading to an increase of the Tm. In turn, GOS containing mono and disaccharides showed a concentration-dependent effect. The results obtained shed light on the mechanisms involved in protection mediated by GOS and lactulose, thus providing support to improve the stabilization of lactic acid bacteria.
Centro de Investigación y Desarrollo en Criotecnología de Alimentos
description Lactulose and galacto-oligosaccharides (GOS) have been recognized as health promoting compounds due to their recognized prebiotic capacity. In addition, because of their polyhydroxilated nature, they are effective protectants during processes involving dehydration of lactic acid bacteria. The protective effects of sugars have been associated with their capacity to replace water molecules from lipid membranes and/or to form glassy matrices. Although this latter property has been previously investigated for GOS and lactulose, the capacity of these sugars to replace water molecules was not. Furthermore, it must be considered that GOS are usually mixtures of oligo-saccharides with differentdegrees of polymerization (DP) including or excluding different amounts of mono and disaccharides, and the effect of their composition on the GOS-membrane interaction has not been addressed so far. The aim of the present work was to study the interaction of lactulose and GOS of different compositions with dipalmitoylphosphatidilcholine (DPPC) membranes both in the gel (Lb) and in the liquid crystalline phase (La) by using infrared spectroscopy. Both GOS and lactulose interacted with the phosphate groups of the polar head region, but only lactulose (disaccharide) penetrated into the lipid bilayers anddecreased the membrane phase transition (Tm). In contrast, GOS containing no mono and disaccharides were excluded from the hydrophobic region, leading to an increase of the Tm. In turn, GOS containing mono and disaccharides showed a concentration-dependent effect. The results obtained shed light on the mechanisms involved in protection mediated by GOS and lactulose, thus providing support to improve the stabilization of lactic acid bacteria.
publishDate 2017
dc.date.none.fl_str_mv 2017-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/99825
url http://sedici.unlp.edu.ar/handle/10915/99825
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://ri.conicet.gov.ar/11336/40842
info:eu-repo/semantics/altIdentifier/url/http://pubs.rsc.org/en/Content/ArticleLanding/2017/RA/C7RA01964E
info:eu-repo/semantics/altIdentifier/issn/2046-2069
info:eu-repo/semantics/altIdentifier/doi/10.1039/C7RA01964E
info:eu-repo/semantics/altIdentifier/hdl/11336/40842
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc/4.0/
Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc/4.0/
Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)
dc.format.none.fl_str_mv application/pdf
24298-24304
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
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