Osteogenic scaffolds based on fumaric/N-isopropylacrylamide copolymers: Designed, properties and biocompatibility studies

Autores
Bravi Costantino, Maria Leticia; Cortizo, María Susana; Cortizo, Ana María; Oberti, Tamara Gisela
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In the present work the creation of a new biomaterial aimed at bone tissue regeneration is shown, covering the synthesis of the polymers, the material design and their exhaustive physicochemical and biological characterization. The copolymers of dioctyl fumarate (DOF) and N-isopropylacrylamide (NIPAM) were synthetized and characterized by size exclusion chromatography, 1H NMR and its thermal properties were studied by DSC and TGA analyses. Based on their properties, two copolymers with different DOF content were selected as raw material to design scaffolds for bone tissue regeneration. The scaffolds were prepared by casting and electrospinning technique, giving rise to biomaterials with particular chemical and topographic surface which were characterized by water contact angle and scanning electron microscopy (SEM) analysis. The cytotoxicity and biodegradation by murine macrophage RAW 264.7 cells was assay, while biocompatibility and osteogenic induction were made using bone marrow progenitor cells (BMPC). None of the biomaterials obtained showed cytotoxicity. The biological properties suggest that the cells adhere and proliferate better on biomaterials with higher DOF content and fibrous morphology. All scaffolds support osteoblastic cell differentiation, but to a greater extent on the biomaterial that has higher DOF content prepared by casting. Altogether, our results suggesting that these biomaterials could be useful in the bone tissue engineering.
Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas
Laboratorio de Investigación en Osteopatías y Metabolismo Mineral
Materia
Química
Biología
fumaric copolymers
thermal properties
fibrous scaffold
biocompatibility
osteoblastic differentiation
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/163964
Acceder
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oai_identifier_str oai:sedici.unlp.edu.ar:10915/163964
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Osteogenic scaffolds based on fumaric/N-isopropylacrylamide copolymers: Designed, properties and biocompatibility studiesBravi Costantino, Maria LeticiaCortizo, María SusanaCortizo, Ana MaríaOberti, Tamara GiselaQuímicaBiologíafumaric copolymersthermal propertiesfibrous scaffoldbiocompatibilityosteoblastic differentiationIn the present work the creation of a new biomaterial aimed at bone tissue regeneration is shown, covering the synthesis of the polymers, the material design and their exhaustive physicochemical and biological characterization. The copolymers of dioctyl fumarate (DOF) and N-isopropylacrylamide (NIPAM) were synthetized and characterized by size exclusion chromatography, 1H NMR and its thermal properties were studied by DSC and TGA analyses. Based on their properties, two copolymers with different DOF content were selected as raw material to design scaffolds for bone tissue regeneration. The scaffolds were prepared by casting and electrospinning technique, giving rise to biomaterials with particular chemical and topographic surface which were characterized by water contact angle and scanning electron microscopy (SEM) analysis. The cytotoxicity and biodegradation by murine macrophage RAW 264.7 cells was assay, while biocompatibility and osteogenic induction were made using bone marrow progenitor cells (BMPC). None of the biomaterials obtained showed cytotoxicity. The biological properties suggest that the cells adhere and proliferate better on biomaterials with higher DOF content and fibrous morphology. All scaffolds support osteoblastic cell differentiation, but to a greater extent on the biomaterial that has higher DOF content prepared by casting. Altogether, our results suggesting that these biomaterials could be useful in the bone tissue engineering.Instituto de Investigaciones Fisicoquímicas Teóricas y AplicadasLaboratorio de Investigación en Osteopatías y Metabolismo Mineral2020-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://sedici.unlp.edu.ar/handle/10915/163964enginfo:eu-repo/semantics/altIdentifier/issn/0014-3057info:eu-repo/semantics/altIdentifier/doi/10.1016/j.eurpolymj.2019.109348info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-10-15T11:34:57Zoai:sedici.unlp.edu.ar:10915/163964Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-10-15 11:34:57.813SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Osteogenic scaffolds based on fumaric/N-isopropylacrylamide copolymers: Designed, properties and biocompatibility studies
title Osteogenic scaffolds based on fumaric/N-isopropylacrylamide copolymers: Designed, properties and biocompatibility studies
spellingShingle Osteogenic scaffolds based on fumaric/N-isopropylacrylamide copolymers: Designed, properties and biocompatibility studies
Bravi Costantino, Maria Leticia
Química
Biología
fumaric copolymers
thermal properties
fibrous scaffold
biocompatibility
osteoblastic differentiation
title_short Osteogenic scaffolds based on fumaric/N-isopropylacrylamide copolymers: Designed, properties and biocompatibility studies
title_full Osteogenic scaffolds based on fumaric/N-isopropylacrylamide copolymers: Designed, properties and biocompatibility studies
title_fullStr Osteogenic scaffolds based on fumaric/N-isopropylacrylamide copolymers: Designed, properties and biocompatibility studies
title_full_unstemmed Osteogenic scaffolds based on fumaric/N-isopropylacrylamide copolymers: Designed, properties and biocompatibility studies
title_sort Osteogenic scaffolds based on fumaric/N-isopropylacrylamide copolymers: Designed, properties and biocompatibility studies
dc.creator.none.fl_str_mv Bravi Costantino, Maria Leticia
Cortizo, María Susana
Cortizo, Ana María
Oberti, Tamara Gisela
author Bravi Costantino, Maria Leticia
author_facet Bravi Costantino, Maria Leticia
Cortizo, María Susana
Cortizo, Ana María
Oberti, Tamara Gisela
author_role author
author2 Cortizo, María Susana
Cortizo, Ana María
Oberti, Tamara Gisela
author2_role author
author
author
dc.subject.none.fl_str_mv Química
Biología
fumaric copolymers
thermal properties
fibrous scaffold
biocompatibility
osteoblastic differentiation
topic Química
Biología
fumaric copolymers
thermal properties
fibrous scaffold
biocompatibility
osteoblastic differentiation
dc.description.none.fl_txt_mv In the present work the creation of a new biomaterial aimed at bone tissue regeneration is shown, covering the synthesis of the polymers, the material design and their exhaustive physicochemical and biological characterization. The copolymers of dioctyl fumarate (DOF) and N-isopropylacrylamide (NIPAM) were synthetized and characterized by size exclusion chromatography, 1H NMR and its thermal properties were studied by DSC and TGA analyses. Based on their properties, two copolymers with different DOF content were selected as raw material to design scaffolds for bone tissue regeneration. The scaffolds were prepared by casting and electrospinning technique, giving rise to biomaterials with particular chemical and topographic surface which were characterized by water contact angle and scanning electron microscopy (SEM) analysis. The cytotoxicity and biodegradation by murine macrophage RAW 264.7 cells was assay, while biocompatibility and osteogenic induction were made using bone marrow progenitor cells (BMPC). None of the biomaterials obtained showed cytotoxicity. The biological properties suggest that the cells adhere and proliferate better on biomaterials with higher DOF content and fibrous morphology. All scaffolds support osteoblastic cell differentiation, but to a greater extent on the biomaterial that has higher DOF content prepared by casting. Altogether, our results suggesting that these biomaterials could be useful in the bone tissue engineering.
Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas
Laboratorio de Investigación en Osteopatías y Metabolismo Mineral
description In the present work the creation of a new biomaterial aimed at bone tissue regeneration is shown, covering the synthesis of the polymers, the material design and their exhaustive physicochemical and biological characterization. The copolymers of dioctyl fumarate (DOF) and N-isopropylacrylamide (NIPAM) were synthetized and characterized by size exclusion chromatography, 1H NMR and its thermal properties were studied by DSC and TGA analyses. Based on their properties, two copolymers with different DOF content were selected as raw material to design scaffolds for bone tissue regeneration. The scaffolds were prepared by casting and electrospinning technique, giving rise to biomaterials with particular chemical and topographic surface which were characterized by water contact angle and scanning electron microscopy (SEM) analysis. The cytotoxicity and biodegradation by murine macrophage RAW 264.7 cells was assay, while biocompatibility and osteogenic induction were made using bone marrow progenitor cells (BMPC). None of the biomaterials obtained showed cytotoxicity. The biological properties suggest that the cells adhere and proliferate better on biomaterials with higher DOF content and fibrous morphology. All scaffolds support osteoblastic cell differentiation, but to a greater extent on the biomaterial that has higher DOF content prepared by casting. Altogether, our results suggesting that these biomaterials could be useful in the bone tissue engineering.
publishDate 2020
dc.date.none.fl_str_mv 2020-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/163964
url http://sedici.unlp.edu.ar/handle/10915/163964
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/0014-3057
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.eurpolymj.2019.109348
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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