Osteogenic scaffolds based on fumaric/N-isopropylacrylamide copolymers: Designed, properties and biocompatibility studies
- Autores
- Bravi Costantino, Maria Leticia; Cortizo, María Susana; Cortizo, Ana María; Oberti, Tamara Gisela
- Año de publicación
- 2020
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- In the present work the creation of a new biomaterial aimed at bone tissue regeneration is shown, covering the synthesis of the polymers, the material design and their exhaustive physicochemical and biological characterization. The copolymers of dioctyl fumarate (DOF) and N-isopropylacrylamide (NIPAM) were synthetized and characterized by size exclusion chromatography, 1H NMR and its thermal properties were studied by DSC and TGA analyses. Based on their properties, two copolymers with different DOF content were selected as raw material to design scaffolds for bone tissue regeneration. The scaffolds were prepared by casting and electrospinning technique, giving rise to biomaterials with particular chemical and topographic surface which were characterized by water contact angle and scanning electron microscopy (SEM) analysis. The cytotoxicity and biodegradation by murine macrophage RAW 264.7 cells was assay, while biocompatibility and osteogenic induction were made using bone marrow progenitor cells (BMPC). None of the biomaterials obtained showed cytotoxicity. The biological properties suggest that the cells adhere and proliferate better on biomaterials with higher DOF content and fibrous morphology. All scaffolds support osteoblastic cell differentiation, but to a greater extent on the biomaterial that has higher DOF content prepared by casting. Altogether, our results suggesting that these biomaterials could be useful in the bone tissue engineering.
Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas
Laboratorio de Investigación en Osteopatías y Metabolismo Mineral - Materia
-
Química
Biología
fumaric copolymers
thermal properties
fibrous scaffold
biocompatibility
osteoblastic differentiation - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by/4.0/
- Repositorio
- Institución
- Universidad Nacional de La Plata
- OAI Identificador
- oai:sedici.unlp.edu.ar:10915/163964
Ver los metadatos del registro completo
id |
SEDICI_a835c0e515bdf62588fa6decf2e1e161 |
---|---|
oai_identifier_str |
oai:sedici.unlp.edu.ar:10915/163964 |
network_acronym_str |
SEDICI |
repository_id_str |
1329 |
network_name_str |
SEDICI (UNLP) |
spelling |
Osteogenic scaffolds based on fumaric/N-isopropylacrylamide copolymers: Designed, properties and biocompatibility studiesBravi Costantino, Maria LeticiaCortizo, María SusanaCortizo, Ana MaríaOberti, Tamara GiselaQuímicaBiologíafumaric copolymersthermal propertiesfibrous scaffoldbiocompatibilityosteoblastic differentiationIn the present work the creation of a new biomaterial aimed at bone tissue regeneration is shown, covering the synthesis of the polymers, the material design and their exhaustive physicochemical and biological characterization. The copolymers of dioctyl fumarate (DOF) and N-isopropylacrylamide (NIPAM) were synthetized and characterized by size exclusion chromatography, 1H NMR and its thermal properties were studied by DSC and TGA analyses. Based on their properties, two copolymers with different DOF content were selected as raw material to design scaffolds for bone tissue regeneration. The scaffolds were prepared by casting and electrospinning technique, giving rise to biomaterials with particular chemical and topographic surface which were characterized by water contact angle and scanning electron microscopy (SEM) analysis. The cytotoxicity and biodegradation by murine macrophage RAW 264.7 cells was assay, while biocompatibility and osteogenic induction were made using bone marrow progenitor cells (BMPC). None of the biomaterials obtained showed cytotoxicity. The biological properties suggest that the cells adhere and proliferate better on biomaterials with higher DOF content and fibrous morphology. All scaffolds support osteoblastic cell differentiation, but to a greater extent on the biomaterial that has higher DOF content prepared by casting. Altogether, our results suggesting that these biomaterials could be useful in the bone tissue engineering.Instituto de Investigaciones Fisicoquímicas Teóricas y AplicadasLaboratorio de Investigación en Osteopatías y Metabolismo Mineral2020-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://sedici.unlp.edu.ar/handle/10915/163964enginfo:eu-repo/semantics/altIdentifier/issn/0014-3057info:eu-repo/semantics/altIdentifier/doi/10.1016/j.eurpolymj.2019.109348info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:43:10Zoai:sedici.unlp.edu.ar:10915/163964Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:43:11.151SEDICI (UNLP) - Universidad Nacional de La Platafalse |
dc.title.none.fl_str_mv |
Osteogenic scaffolds based on fumaric/N-isopropylacrylamide copolymers: Designed, properties and biocompatibility studies |
title |
Osteogenic scaffolds based on fumaric/N-isopropylacrylamide copolymers: Designed, properties and biocompatibility studies |
spellingShingle |
Osteogenic scaffolds based on fumaric/N-isopropylacrylamide copolymers: Designed, properties and biocompatibility studies Bravi Costantino, Maria Leticia Química Biología fumaric copolymers thermal properties fibrous scaffold biocompatibility osteoblastic differentiation |
title_short |
Osteogenic scaffolds based on fumaric/N-isopropylacrylamide copolymers: Designed, properties and biocompatibility studies |
title_full |
Osteogenic scaffolds based on fumaric/N-isopropylacrylamide copolymers: Designed, properties and biocompatibility studies |
title_fullStr |
Osteogenic scaffolds based on fumaric/N-isopropylacrylamide copolymers: Designed, properties and biocompatibility studies |
title_full_unstemmed |
Osteogenic scaffolds based on fumaric/N-isopropylacrylamide copolymers: Designed, properties and biocompatibility studies |
title_sort |
Osteogenic scaffolds based on fumaric/N-isopropylacrylamide copolymers: Designed, properties and biocompatibility studies |
dc.creator.none.fl_str_mv |
Bravi Costantino, Maria Leticia Cortizo, María Susana Cortizo, Ana María Oberti, Tamara Gisela |
author |
Bravi Costantino, Maria Leticia |
author_facet |
Bravi Costantino, Maria Leticia Cortizo, María Susana Cortizo, Ana María Oberti, Tamara Gisela |
author_role |
author |
author2 |
Cortizo, María Susana Cortizo, Ana María Oberti, Tamara Gisela |
author2_role |
author author author |
dc.subject.none.fl_str_mv |
Química Biología fumaric copolymers thermal properties fibrous scaffold biocompatibility osteoblastic differentiation |
topic |
Química Biología fumaric copolymers thermal properties fibrous scaffold biocompatibility osteoblastic differentiation |
dc.description.none.fl_txt_mv |
In the present work the creation of a new biomaterial aimed at bone tissue regeneration is shown, covering the synthesis of the polymers, the material design and their exhaustive physicochemical and biological characterization. The copolymers of dioctyl fumarate (DOF) and N-isopropylacrylamide (NIPAM) were synthetized and characterized by size exclusion chromatography, 1H NMR and its thermal properties were studied by DSC and TGA analyses. Based on their properties, two copolymers with different DOF content were selected as raw material to design scaffolds for bone tissue regeneration. The scaffolds were prepared by casting and electrospinning technique, giving rise to biomaterials with particular chemical and topographic surface which were characterized by water contact angle and scanning electron microscopy (SEM) analysis. The cytotoxicity and biodegradation by murine macrophage RAW 264.7 cells was assay, while biocompatibility and osteogenic induction were made using bone marrow progenitor cells (BMPC). None of the biomaterials obtained showed cytotoxicity. The biological properties suggest that the cells adhere and proliferate better on biomaterials with higher DOF content and fibrous morphology. All scaffolds support osteoblastic cell differentiation, but to a greater extent on the biomaterial that has higher DOF content prepared by casting. Altogether, our results suggesting that these biomaterials could be useful in the bone tissue engineering. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas Laboratorio de Investigación en Osteopatías y Metabolismo Mineral |
description |
In the present work the creation of a new biomaterial aimed at bone tissue regeneration is shown, covering the synthesis of the polymers, the material design and their exhaustive physicochemical and biological characterization. The copolymers of dioctyl fumarate (DOF) and N-isopropylacrylamide (NIPAM) were synthetized and characterized by size exclusion chromatography, 1H NMR and its thermal properties were studied by DSC and TGA analyses. Based on their properties, two copolymers with different DOF content were selected as raw material to design scaffolds for bone tissue regeneration. The scaffolds were prepared by casting and electrospinning technique, giving rise to biomaterials with particular chemical and topographic surface which were characterized by water contact angle and scanning electron microscopy (SEM) analysis. The cytotoxicity and biodegradation by murine macrophage RAW 264.7 cells was assay, while biocompatibility and osteogenic induction were made using bone marrow progenitor cells (BMPC). None of the biomaterials obtained showed cytotoxicity. The biological properties suggest that the cells adhere and proliferate better on biomaterials with higher DOF content and fibrous morphology. All scaffolds support osteoblastic cell differentiation, but to a greater extent on the biomaterial that has higher DOF content prepared by casting. Altogether, our results suggesting that these biomaterials could be useful in the bone tissue engineering. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-01 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Articulo http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://sedici.unlp.edu.ar/handle/10915/163964 |
url |
http://sedici.unlp.edu.ar/handle/10915/163964 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/issn/0014-3057 info:eu-repo/semantics/altIdentifier/doi/10.1016/j.eurpolymj.2019.109348 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/4.0/ Creative Commons Attribution 4.0 International (CC BY 4.0) |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by/4.0/ Creative Commons Attribution 4.0 International (CC BY 4.0) |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:SEDICI (UNLP) instname:Universidad Nacional de La Plata instacron:UNLP |
reponame_str |
SEDICI (UNLP) |
collection |
SEDICI (UNLP) |
instname_str |
Universidad Nacional de La Plata |
instacron_str |
UNLP |
institution |
UNLP |
repository.name.fl_str_mv |
SEDICI (UNLP) - Universidad Nacional de La Plata |
repository.mail.fl_str_mv |
alira@sedici.unlp.edu.ar |
_version_ |
1844616300569034752 |
score |
13.070432 |