The mitochondrial genome of the plantpathogenic fungus <i>Stemphylium lycopersici</i> uncovers a dynamic structure due to repetitive and mobile elements

Autores
Franco, Mario Emilio Ernesto; López, Silvina Marianela Yanil; Medina, Rocío; Lucentini, César Gustavo; Troncozo, María Inés; Pastorino, Graciela Noemí; Saparrat, Mario Carlos Nazareno; Balatti, Pedro Alberto
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Stemphylium lycopersici (Pleosporales) is a plant-pathogenic fungus that has been associated with a broad range of plant-hosts worldwide. It is one of the causative agents of gray leaf spot disease in tomato and pepper. The aim of this work was to characterize the mitochondrial genome of S. lycopersici CIDEFI-216, to use it to trace taxonomic relationships with other fungal taxa and to get insights into the evolutionary history of this phytopathogen. The complete mitochondrial genome was assembled into a circular double-stranded DNA molecule of 75,911 bp that harbors a set of 37 protein-coding genes, 2 rRNA genes (rns and rnl) and 28 tRNA genes, which are transcribed from both sense and antisense strands. Remarkably, its gene repertoire lacks both atp8 and atp9, contains a free-standing gene for the ribosomal protein S3 (rps3) and includes 13 genes with homing endonuclease domains that are mostly located within its 15 group I introns. Strikingly, subunits 1 and 2 of cytochrome oxidase are encoded by a single continuous open reading frame (ORF). A comparative mitogenomic analysis revealed the large extent of structural rearrangements among representatives of Pleosporales, showing the plasticity of their mitochondrial genomes. Finally, an exhaustive phylogenetic analysis of the subphylum Pezizomycotina based on mitochondrial data reconstructed their relationships in concordance with several studies based on nuclear data. This is the first report of a mitochondrial genome belonging to a representative of the family Pleosporaceae.
Facultad de Ciencias Agrarias y Forestales
Materia
Ciencias Agrarias
Hongos
Enfermedades de las Plantas
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/66666
Acceder
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network_name_str SEDICI (UNLP)
spelling The mitochondrial genome of the plantpathogenic fungus <i>Stemphylium lycopersici</i> uncovers a dynamic structure due to repetitive and mobile elementsFranco, Mario Emilio ErnestoLópez, Silvina Marianela YanilMedina, RocíoLucentini, César GustavoTroncozo, María InésPastorino, Graciela NoemíSaparrat, Mario Carlos NazarenoBalatti, Pedro AlbertoCiencias AgrariasHongosEnfermedades de las PlantasStemphylium lycopersici (Pleosporales) is a plant-pathogenic fungus that has been associated with a broad range of plant-hosts worldwide. It is one of the causative agents of gray leaf spot disease in tomato and pepper. The aim of this work was to characterize the mitochondrial genome of S. lycopersici CIDEFI-216, to use it to trace taxonomic relationships with other fungal taxa and to get insights into the evolutionary history of this phytopathogen. The complete mitochondrial genome was assembled into a circular double-stranded DNA molecule of 75,911 bp that harbors a set of 37 protein-coding genes, 2 rRNA genes (rns and rnl) and 28 tRNA genes, which are transcribed from both sense and antisense strands. Remarkably, its gene repertoire lacks both atp8 and atp9, contains a free-standing gene for the ribosomal protein S3 (rps3) and includes 13 genes with homing endonuclease domains that are mostly located within its 15 group I introns. Strikingly, subunits 1 and 2 of cytochrome oxidase are encoded by a single continuous open reading frame (ORF). A comparative mitogenomic analysis revealed the large extent of structural rearrangements among representatives of Pleosporales, showing the plasticity of their mitochondrial genomes. Finally, an exhaustive phylogenetic analysis of the subphylum Pezizomycotina based on mitochondrial data reconstructed their relationships in concordance with several studies based on nuclear data. This is the first report of a mitochondrial genome belonging to a representative of the family Pleosporaceae.Facultad de Ciencias Agrarias y Forestales2017-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://sedici.unlp.edu.ar/handle/10915/66666enginfo:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0185545&type=printableinfo:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0185545info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-10-22T16:50:56Zoai:sedici.unlp.edu.ar:10915/66666Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-10-22 16:50:56.384SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv The mitochondrial genome of the plantpathogenic fungus <i>Stemphylium lycopersici</i> uncovers a dynamic structure due to repetitive and mobile elements
title The mitochondrial genome of the plantpathogenic fungus <i>Stemphylium lycopersici</i> uncovers a dynamic structure due to repetitive and mobile elements
spellingShingle The mitochondrial genome of the plantpathogenic fungus <i>Stemphylium lycopersici</i> uncovers a dynamic structure due to repetitive and mobile elements
Franco, Mario Emilio Ernesto
Ciencias Agrarias
Hongos
Enfermedades de las Plantas
title_short The mitochondrial genome of the plantpathogenic fungus <i>Stemphylium lycopersici</i> uncovers a dynamic structure due to repetitive and mobile elements
title_full The mitochondrial genome of the plantpathogenic fungus <i>Stemphylium lycopersici</i> uncovers a dynamic structure due to repetitive and mobile elements
title_fullStr The mitochondrial genome of the plantpathogenic fungus <i>Stemphylium lycopersici</i> uncovers a dynamic structure due to repetitive and mobile elements
title_full_unstemmed The mitochondrial genome of the plantpathogenic fungus <i>Stemphylium lycopersici</i> uncovers a dynamic structure due to repetitive and mobile elements
title_sort The mitochondrial genome of the plantpathogenic fungus <i>Stemphylium lycopersici</i> uncovers a dynamic structure due to repetitive and mobile elements
dc.creator.none.fl_str_mv Franco, Mario Emilio Ernesto
López, Silvina Marianela Yanil
Medina, Rocío
Lucentini, César Gustavo
Troncozo, María Inés
Pastorino, Graciela Noemí
Saparrat, Mario Carlos Nazareno
Balatti, Pedro Alberto
author Franco, Mario Emilio Ernesto
author_facet Franco, Mario Emilio Ernesto
López, Silvina Marianela Yanil
Medina, Rocío
Lucentini, César Gustavo
Troncozo, María Inés
Pastorino, Graciela Noemí
Saparrat, Mario Carlos Nazareno
Balatti, Pedro Alberto
author_role author
author2 López, Silvina Marianela Yanil
Medina, Rocío
Lucentini, César Gustavo
Troncozo, María Inés
Pastorino, Graciela Noemí
Saparrat, Mario Carlos Nazareno
Balatti, Pedro Alberto
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Ciencias Agrarias
Hongos
Enfermedades de las Plantas
topic Ciencias Agrarias
Hongos
Enfermedades de las Plantas
dc.description.none.fl_txt_mv Stemphylium lycopersici (Pleosporales) is a plant-pathogenic fungus that has been associated with a broad range of plant-hosts worldwide. It is one of the causative agents of gray leaf spot disease in tomato and pepper. The aim of this work was to characterize the mitochondrial genome of S. lycopersici CIDEFI-216, to use it to trace taxonomic relationships with other fungal taxa and to get insights into the evolutionary history of this phytopathogen. The complete mitochondrial genome was assembled into a circular double-stranded DNA molecule of 75,911 bp that harbors a set of 37 protein-coding genes, 2 rRNA genes (rns and rnl) and 28 tRNA genes, which are transcribed from both sense and antisense strands. Remarkably, its gene repertoire lacks both atp8 and atp9, contains a free-standing gene for the ribosomal protein S3 (rps3) and includes 13 genes with homing endonuclease domains that are mostly located within its 15 group I introns. Strikingly, subunits 1 and 2 of cytochrome oxidase are encoded by a single continuous open reading frame (ORF). A comparative mitogenomic analysis revealed the large extent of structural rearrangements among representatives of Pleosporales, showing the plasticity of their mitochondrial genomes. Finally, an exhaustive phylogenetic analysis of the subphylum Pezizomycotina based on mitochondrial data reconstructed their relationships in concordance with several studies based on nuclear data. This is the first report of a mitochondrial genome belonging to a representative of the family Pleosporaceae.
Facultad de Ciencias Agrarias y Forestales
description Stemphylium lycopersici (Pleosporales) is a plant-pathogenic fungus that has been associated with a broad range of plant-hosts worldwide. It is one of the causative agents of gray leaf spot disease in tomato and pepper. The aim of this work was to characterize the mitochondrial genome of S. lycopersici CIDEFI-216, to use it to trace taxonomic relationships with other fungal taxa and to get insights into the evolutionary history of this phytopathogen. The complete mitochondrial genome was assembled into a circular double-stranded DNA molecule of 75,911 bp that harbors a set of 37 protein-coding genes, 2 rRNA genes (rns and rnl) and 28 tRNA genes, which are transcribed from both sense and antisense strands. Remarkably, its gene repertoire lacks both atp8 and atp9, contains a free-standing gene for the ribosomal protein S3 (rps3) and includes 13 genes with homing endonuclease domains that are mostly located within its 15 group I introns. Strikingly, subunits 1 and 2 of cytochrome oxidase are encoded by a single continuous open reading frame (ORF). A comparative mitogenomic analysis revealed the large extent of structural rearrangements among representatives of Pleosporales, showing the plasticity of their mitochondrial genomes. Finally, an exhaustive phylogenetic analysis of the subphylum Pezizomycotina based on mitochondrial data reconstructed their relationships in concordance with several studies based on nuclear data. This is the first report of a mitochondrial genome belonging to a representative of the family Pleosporaceae.
publishDate 2017
dc.date.none.fl_str_mv 2017-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
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info:ar-repo/semantics/articulo
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status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/66666
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dc.language.none.fl_str_mv eng
language eng
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info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0185545
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
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Creative Commons Attribution 4.0 International (CC BY 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
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dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
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