Draft Genome Sequence of the Plant-Pathogenic Fungus <i>Stemphylium lycopersici</i> Strain CIDEFI-216

Autores
Franco, Mario Emilio Ernesto; López, Silvina Marianela Yanil; Medina, Rocío; Saparrat, Mario Carlos Nazareno; Balatti, Pedro Alberto
Año de publicación
2015
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Stemphylium lycopersiciis a pathogenic fungus that provokes the leaf spot disease on over 30 host genera worldwide, among them Tomato. Here we report the draft genome sequence ofS. lycopersicistrain CIDEFI-216. Total genomic DNA was isolated from a monosporic culture using the DNeasy Plant Mini Kit (Qiagen) and was used to construct 100 bp paired-end libraries, which were then sequenced by Illumina HiSeq 2000 technology. Reads were assembled using SOAPdenovo2 software at Macrogen (Korea).Gene prediction was performed by Fgenesh software (Softberry), functional annotation was carried out with Blast2GO software (BioBam) and tRNAs and rRNAs were predicted usingtRNAscan-SE and HMM-rRNA tools from WebMGA server. The paired-end libraries produced 31117554 reads with a total of 3142872954 bp, representing an average coverage of 77.39 X. The genome was assembled into 419 scaffolds with a total length of 35.18 Mbp (1000 bp; N50=498048 bp) and an overall G+C content of 50.5 %. A total of 8998 protein-coding genes were predicted, whose functional annotation is discussed. Additionally, 94 tRNAs and 44 rRNAs were found. This draft genome sequence, the first available forS. lycopersici, represents a new resource for further research into the taxonomy, biology and phytopathology of this plant pathogen.
Facultad de Ciencias Agrarias y Forestales
Materia
Ciencias Agrarias
Enfermedades de las Plantas
Hongos
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-nd/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/66623
Acceder
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network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Draft Genome Sequence of the Plant-Pathogenic Fungus <i>Stemphylium lycopersici</i> Strain CIDEFI-216Franco, Mario Emilio ErnestoLópez, Silvina Marianela YanilMedina, RocíoSaparrat, Mario Carlos NazarenoBalatti, Pedro AlbertoCiencias AgrariasEnfermedades de las PlantasHongosStemphylium lycopersiciis a pathogenic fungus that provokes the leaf spot disease on over 30 host genera worldwide, among them Tomato. Here we report the draft genome sequence ofS. lycopersicistrain CIDEFI-216. Total genomic DNA was isolated from a monosporic culture using the DNeasy Plant Mini Kit (Qiagen) and was used to construct 100 bp paired-end libraries, which were then sequenced by Illumina HiSeq 2000 technology. Reads were assembled using SOAPdenovo2 software at Macrogen (Korea).Gene prediction was performed by Fgenesh software (Softberry), functional annotation was carried out with Blast2GO software (BioBam) and tRNAs and rRNAs were predicted usingtRNAscan-SE and HMM-rRNA tools from WebMGA server. The paired-end libraries produced 31117554 reads with a total of 3142872954 bp, representing an average coverage of 77.39 X. The genome was assembled into 419 scaffolds with a total length of 35.18 Mbp (1000 bp; N50=498048 bp) and an overall G+C content of 50.5 %. A total of 8998 protein-coding genes were predicted, whose functional annotation is discussed. Additionally, 94 tRNAs and 44 rRNAs were found. This draft genome sequence, the first available forS. lycopersici, represents a new resource for further research into the taxonomy, biology and phytopathology of this plant pathogen.Facultad de Ciencias Agrarias y Forestales2015info:eu-repo/semantics/conferenceObjectinfo:eu-repo/semantics/publishedVersionObjeto de conferenciahttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfhttp://sedici.unlp.edu.ar/handle/10915/66623enginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/4.0/Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:10:00Zoai:sedici.unlp.edu.ar:10915/66623Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:10:00.537SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Draft Genome Sequence of the Plant-Pathogenic Fungus <i>Stemphylium lycopersici</i> Strain CIDEFI-216
title Draft Genome Sequence of the Plant-Pathogenic Fungus <i>Stemphylium lycopersici</i> Strain CIDEFI-216
spellingShingle Draft Genome Sequence of the Plant-Pathogenic Fungus <i>Stemphylium lycopersici</i> Strain CIDEFI-216
Franco, Mario Emilio Ernesto
Ciencias Agrarias
Enfermedades de las Plantas
Hongos
title_short Draft Genome Sequence of the Plant-Pathogenic Fungus <i>Stemphylium lycopersici</i> Strain CIDEFI-216
title_full Draft Genome Sequence of the Plant-Pathogenic Fungus <i>Stemphylium lycopersici</i> Strain CIDEFI-216
title_fullStr Draft Genome Sequence of the Plant-Pathogenic Fungus <i>Stemphylium lycopersici</i> Strain CIDEFI-216
title_full_unstemmed Draft Genome Sequence of the Plant-Pathogenic Fungus <i>Stemphylium lycopersici</i> Strain CIDEFI-216
title_sort Draft Genome Sequence of the Plant-Pathogenic Fungus <i>Stemphylium lycopersici</i> Strain CIDEFI-216
dc.creator.none.fl_str_mv Franco, Mario Emilio Ernesto
López, Silvina Marianela Yanil
Medina, Rocío
Saparrat, Mario Carlos Nazareno
Balatti, Pedro Alberto
author Franco, Mario Emilio Ernesto
author_facet Franco, Mario Emilio Ernesto
López, Silvina Marianela Yanil
Medina, Rocío
Saparrat, Mario Carlos Nazareno
Balatti, Pedro Alberto
author_role author
author2 López, Silvina Marianela Yanil
Medina, Rocío
Saparrat, Mario Carlos Nazareno
Balatti, Pedro Alberto
author2_role author
author
author
author
dc.subject.none.fl_str_mv Ciencias Agrarias
Enfermedades de las Plantas
Hongos
topic Ciencias Agrarias
Enfermedades de las Plantas
Hongos
dc.description.none.fl_txt_mv Stemphylium lycopersiciis a pathogenic fungus that provokes the leaf spot disease on over 30 host genera worldwide, among them Tomato. Here we report the draft genome sequence ofS. lycopersicistrain CIDEFI-216. Total genomic DNA was isolated from a monosporic culture using the DNeasy Plant Mini Kit (Qiagen) and was used to construct 100 bp paired-end libraries, which were then sequenced by Illumina HiSeq 2000 technology. Reads were assembled using SOAPdenovo2 software at Macrogen (Korea).Gene prediction was performed by Fgenesh software (Softberry), functional annotation was carried out with Blast2GO software (BioBam) and tRNAs and rRNAs were predicted usingtRNAscan-SE and HMM-rRNA tools from WebMGA server. The paired-end libraries produced 31117554 reads with a total of 3142872954 bp, representing an average coverage of 77.39 X. The genome was assembled into 419 scaffolds with a total length of 35.18 Mbp (1000 bp; N50=498048 bp) and an overall G+C content of 50.5 %. A total of 8998 protein-coding genes were predicted, whose functional annotation is discussed. Additionally, 94 tRNAs and 44 rRNAs were found. This draft genome sequence, the first available forS. lycopersici, represents a new resource for further research into the taxonomy, biology and phytopathology of this plant pathogen.
Facultad de Ciencias Agrarias y Forestales
description Stemphylium lycopersiciis a pathogenic fungus that provokes the leaf spot disease on over 30 host genera worldwide, among them Tomato. Here we report the draft genome sequence ofS. lycopersicistrain CIDEFI-216. Total genomic DNA was isolated from a monosporic culture using the DNeasy Plant Mini Kit (Qiagen) and was used to construct 100 bp paired-end libraries, which were then sequenced by Illumina HiSeq 2000 technology. Reads were assembled using SOAPdenovo2 software at Macrogen (Korea).Gene prediction was performed by Fgenesh software (Softberry), functional annotation was carried out with Blast2GO software (BioBam) and tRNAs and rRNAs were predicted usingtRNAscan-SE and HMM-rRNA tools from WebMGA server. The paired-end libraries produced 31117554 reads with a total of 3142872954 bp, representing an average coverage of 77.39 X. The genome was assembled into 419 scaffolds with a total length of 35.18 Mbp (1000 bp; N50=498048 bp) and an overall G+C content of 50.5 %. A total of 8998 protein-coding genes were predicted, whose functional annotation is discussed. Additionally, 94 tRNAs and 44 rRNAs were found. This draft genome sequence, the first available forS. lycopersici, represents a new resource for further research into the taxonomy, biology and phytopathology of this plant pathogen.
publishDate 2015
dc.date.none.fl_str_mv 2015
dc.type.none.fl_str_mv info:eu-repo/semantics/conferenceObject
info:eu-repo/semantics/publishedVersion
Objeto de conferencia
http://purl.org/coar/resource_type/c_5794
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dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/66623
url http://sedici.unlp.edu.ar/handle/10915/66623
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-nd/4.0/
Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-nd/4.0/
Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
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