Genetic characterisation of PPARG, CEBPA and RXRA, and their influence on meat quality traits in cattle

Autores
Goszczynski, Daniel Estanislao; Papaleo Mazzucco, Juliana; Ripoli, María Verónica; Villarreal, Edgardo Leopoldo; Rogberg Muñoz, Andrés; Mezzadra, Carlos Alberto; Melucci, Lilia Magdalena; Giovambattista, Guillermo
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: Peroxisome proliferator-activated receptor gamma (PPARG), CCAAT/enhancer binding protein alpha (CEBPA) and retinoid X receptor alpha (RXRA) are nuclear transcription factors that play important roles in regulation of adipogenesis and fat deposition. The objectives of this study were to characterise the variability of these three candidate genes in a mixed sample panel composed of several cattle breeds with different meat quality, validate single nucleotide polymorphisms (SNPs) in a local crossbred population (Angus - Hereford - Limousin) and evaluate their effects on meat quality traits (backfat thickness, intramuscular fat content and fatty acid composition), supporting the association tests with bioinformatic predictive studies. Results: Globally, nine SNPs were detected in the PPARG and CEBPA genes within our mixed panel, including a novel SNP in the latter. Three of these nine, along with seven other SNPs selected from the Single Nucleotide Polymorphism database (SNPdb), including SNPs in the RXRA gene, were validated in the crossbred population (N = 260). After validation, five of these SNPs were evaluated for genotype effects on fatty acid content and composition. Significant effects were observed on backfat thickness and different fatty acid contents (P < 0.05). Some of these SNPs caused slight differences in mRNA structure stability and/or putative binding sites for proteins. Conclusions: PPARG and CEBPA showed low to moderate variability in our sample panel. Variations in these genes, along with RXRA, may explain part of the genetic variation in fat content and composition. Our results may contribute to knowledge about genetic variation in meat quality traits in cattle and should be evaluated in larger independent populations.
Instituto de Genética Veterinaria
Materia
Ciencias Veterinarias
Polymorphism
Variation
Association
Cows
Beef
Snp
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/100993

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repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Genetic characterisation of PPARG, CEBPA and RXRA, and their influence on meat quality traits in cattleGoszczynski, Daniel EstanislaoPapaleo Mazzucco, JulianaRipoli, María VerónicaVillarreal, Edgardo LeopoldoRogberg Muñoz, AndrésMezzadra, Carlos AlbertoMelucci, Lilia MagdalenaGiovambattista, GuillermoCiencias VeterinariasPolymorphismVariationAssociationCowsBeefSnpBackground: Peroxisome proliferator-activated receptor gamma (PPARG), CCAAT/enhancer binding protein alpha (CEBPA) and retinoid X receptor alpha (RXRA) are nuclear transcription factors that play important roles in regulation of adipogenesis and fat deposition. The objectives of this study were to characterise the variability of these three candidate genes in a mixed sample panel composed of several cattle breeds with different meat quality, validate single nucleotide polymorphisms (SNPs) in a local crossbred population (Angus - Hereford - Limousin) and evaluate their effects on meat quality traits (backfat thickness, intramuscular fat content and fatty acid composition), supporting the association tests with bioinformatic predictive studies. Results: Globally, nine SNPs were detected in the PPARG and CEBPA genes within our mixed panel, including a novel SNP in the latter. Three of these nine, along with seven other SNPs selected from the Single Nucleotide Polymorphism database (SNPdb), including SNPs in the RXRA gene, were validated in the crossbred population (N = 260). After validation, five of these SNPs were evaluated for genotype effects on fatty acid content and composition. Significant effects were observed on backfat thickness and different fatty acid contents (P < 0.05). Some of these SNPs caused slight differences in mRNA structure stability and/or putative binding sites for proteins. Conclusions: PPARG and CEBPA showed low to moderate variability in our sample panel. Variations in these genes, along with RXRA, may explain part of the genetic variation in fat content and composition. Our results may contribute to knowledge about genetic variation in meat quality traits in cattle and should be evaluated in larger independent populations.Instituto de Genética Veterinaria2016-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf1-9http://sedici.unlp.edu.ar/handle/10915/100993enginfo:eu-repo/semantics/altIdentifier/url/https://ri.conicet.gov.ar/11336/48668info:eu-repo/semantics/altIdentifier/issn/2055-0391info:eu-repo/semantics/altIdentifier/doi/10.1186/s40781-016-0095-3info:eu-repo/semantics/altIdentifier/hdl/11336/48668info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:20:05Zoai:sedici.unlp.edu.ar:10915/100993Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:20:06.113SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Genetic characterisation of PPARG, CEBPA and RXRA, and their influence on meat quality traits in cattle
title Genetic characterisation of PPARG, CEBPA and RXRA, and their influence on meat quality traits in cattle
spellingShingle Genetic characterisation of PPARG, CEBPA and RXRA, and their influence on meat quality traits in cattle
Goszczynski, Daniel Estanislao
Ciencias Veterinarias
Polymorphism
Variation
Association
Cows
Beef
Snp
title_short Genetic characterisation of PPARG, CEBPA and RXRA, and their influence on meat quality traits in cattle
title_full Genetic characterisation of PPARG, CEBPA and RXRA, and their influence on meat quality traits in cattle
title_fullStr Genetic characterisation of PPARG, CEBPA and RXRA, and their influence on meat quality traits in cattle
title_full_unstemmed Genetic characterisation of PPARG, CEBPA and RXRA, and their influence on meat quality traits in cattle
title_sort Genetic characterisation of PPARG, CEBPA and RXRA, and their influence on meat quality traits in cattle
dc.creator.none.fl_str_mv Goszczynski, Daniel Estanislao
Papaleo Mazzucco, Juliana
Ripoli, María Verónica
Villarreal, Edgardo Leopoldo
Rogberg Muñoz, Andrés
Mezzadra, Carlos Alberto
Melucci, Lilia Magdalena
Giovambattista, Guillermo
author Goszczynski, Daniel Estanislao
author_facet Goszczynski, Daniel Estanislao
Papaleo Mazzucco, Juliana
Ripoli, María Verónica
Villarreal, Edgardo Leopoldo
Rogberg Muñoz, Andrés
Mezzadra, Carlos Alberto
Melucci, Lilia Magdalena
Giovambattista, Guillermo
author_role author
author2 Papaleo Mazzucco, Juliana
Ripoli, María Verónica
Villarreal, Edgardo Leopoldo
Rogberg Muñoz, Andrés
Mezzadra, Carlos Alberto
Melucci, Lilia Magdalena
Giovambattista, Guillermo
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Ciencias Veterinarias
Polymorphism
Variation
Association
Cows
Beef
Snp
topic Ciencias Veterinarias
Polymorphism
Variation
Association
Cows
Beef
Snp
dc.description.none.fl_txt_mv Background: Peroxisome proliferator-activated receptor gamma (PPARG), CCAAT/enhancer binding protein alpha (CEBPA) and retinoid X receptor alpha (RXRA) are nuclear transcription factors that play important roles in regulation of adipogenesis and fat deposition. The objectives of this study were to characterise the variability of these three candidate genes in a mixed sample panel composed of several cattle breeds with different meat quality, validate single nucleotide polymorphisms (SNPs) in a local crossbred population (Angus - Hereford - Limousin) and evaluate their effects on meat quality traits (backfat thickness, intramuscular fat content and fatty acid composition), supporting the association tests with bioinformatic predictive studies. Results: Globally, nine SNPs were detected in the PPARG and CEBPA genes within our mixed panel, including a novel SNP in the latter. Three of these nine, along with seven other SNPs selected from the Single Nucleotide Polymorphism database (SNPdb), including SNPs in the RXRA gene, were validated in the crossbred population (N = 260). After validation, five of these SNPs were evaluated for genotype effects on fatty acid content and composition. Significant effects were observed on backfat thickness and different fatty acid contents (P < 0.05). Some of these SNPs caused slight differences in mRNA structure stability and/or putative binding sites for proteins. Conclusions: PPARG and CEBPA showed low to moderate variability in our sample panel. Variations in these genes, along with RXRA, may explain part of the genetic variation in fat content and composition. Our results may contribute to knowledge about genetic variation in meat quality traits in cattle and should be evaluated in larger independent populations.
Instituto de Genética Veterinaria
description Background: Peroxisome proliferator-activated receptor gamma (PPARG), CCAAT/enhancer binding protein alpha (CEBPA) and retinoid X receptor alpha (RXRA) are nuclear transcription factors that play important roles in regulation of adipogenesis and fat deposition. The objectives of this study were to characterise the variability of these three candidate genes in a mixed sample panel composed of several cattle breeds with different meat quality, validate single nucleotide polymorphisms (SNPs) in a local crossbred population (Angus - Hereford - Limousin) and evaluate their effects on meat quality traits (backfat thickness, intramuscular fat content and fatty acid composition), supporting the association tests with bioinformatic predictive studies. Results: Globally, nine SNPs were detected in the PPARG and CEBPA genes within our mixed panel, including a novel SNP in the latter. Three of these nine, along with seven other SNPs selected from the Single Nucleotide Polymorphism database (SNPdb), including SNPs in the RXRA gene, were validated in the crossbred population (N = 260). After validation, five of these SNPs were evaluated for genotype effects on fatty acid content and composition. Significant effects were observed on backfat thickness and different fatty acid contents (P < 0.05). Some of these SNPs caused slight differences in mRNA structure stability and/or putative binding sites for proteins. Conclusions: PPARG and CEBPA showed low to moderate variability in our sample panel. Variations in these genes, along with RXRA, may explain part of the genetic variation in fat content and composition. Our results may contribute to knowledge about genetic variation in meat quality traits in cattle and should be evaluated in larger independent populations.
publishDate 2016
dc.date.none.fl_str_mv 2016-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/100993
url http://sedici.unlp.edu.ar/handle/10915/100993
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://ri.conicet.gov.ar/11336/48668
info:eu-repo/semantics/altIdentifier/issn/2055-0391
info:eu-repo/semantics/altIdentifier/doi/10.1186/s40781-016-0095-3
info:eu-repo/semantics/altIdentifier/hdl/11336/48668
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
1-9
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
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reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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