Genetic characterisation of PPARG, CEBPA and RXRA, and their influence on meat quality traits in cattle
- Autores
- Goszczynski, Daniel Estanislao; Papaleo Mazzucco, Juliana; Ripoli, María Verónica; Villarreal, Edgardo Leopoldo; Rogberg Muñoz, Andrés; Mezzadra, Carlos Alberto; Melucci, Lilia Magdalena; Giovambattista, Guillermo
- Año de publicación
- 2016
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Background: Peroxisome proliferator-activated receptor gamma (PPARG), CCAAT/enhancer binding protein alpha (CEBPA) and retinoid X receptor alpha (RXRA) are nuclear transcription factors that play important roles in regulation of adipogenesis and fat deposition. The objectives of this study were to characterise the variability of these three candidate genes in a mixed sample panel composed of several cattle breeds with different meat quality, validate single nucleotide polymorphisms (SNPs) in a local crossbred population (Angus - Hereford - Limousin) and evaluate their effects on meat quality traits (backfat thickness, intramuscular fat content and fatty acid composition), supporting the association tests with bioinformatic predictive studies. Results: Globally, nine SNPs were detected in the PPARG and CEBPA genes within our mixed panel, including a novel SNP in the latter. Three of these nine, along with seven other SNPs selected from the Single Nucleotide Polymorphism database (SNPdb), including SNPs in the RXRA gene, were validated in the crossbred population (N = 260). After validation, five of these SNPs were evaluated for genotype effects on fatty acid content and composition. Significant effects were observed on backfat thickness and different fatty acid contents (P < 0.05). Some of these SNPs caused slight differences in mRNA structure stability and/or putative binding sites for proteins. Conclusions: PPARG and CEBPA showed low to moderate variability in our sample panel. Variations in these genes, along with RXRA, may explain part of the genetic variation in fat content and composition. Our results may contribute to knowledge about genetic variation in meat quality traits in cattle and should be evaluated in larger independent populations.
Instituto de Genética Veterinaria - Materia
-
Ciencias Veterinarias
Polymorphism
Variation
Association
Cows
Beef
Snp - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by-nc-sa/4.0/
- Repositorio
- Institución
- Universidad Nacional de La Plata
- OAI Identificador
- oai:sedici.unlp.edu.ar:10915/100993
Ver los metadatos del registro completo
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Genetic characterisation of PPARG, CEBPA and RXRA, and their influence on meat quality traits in cattleGoszczynski, Daniel EstanislaoPapaleo Mazzucco, JulianaRipoli, María VerónicaVillarreal, Edgardo LeopoldoRogberg Muñoz, AndrésMezzadra, Carlos AlbertoMelucci, Lilia MagdalenaGiovambattista, GuillermoCiencias VeterinariasPolymorphismVariationAssociationCowsBeefSnpBackground: Peroxisome proliferator-activated receptor gamma (PPARG), CCAAT/enhancer binding protein alpha (CEBPA) and retinoid X receptor alpha (RXRA) are nuclear transcription factors that play important roles in regulation of adipogenesis and fat deposition. The objectives of this study were to characterise the variability of these three candidate genes in a mixed sample panel composed of several cattle breeds with different meat quality, validate single nucleotide polymorphisms (SNPs) in a local crossbred population (Angus - Hereford - Limousin) and evaluate their effects on meat quality traits (backfat thickness, intramuscular fat content and fatty acid composition), supporting the association tests with bioinformatic predictive studies. Results: Globally, nine SNPs were detected in the PPARG and CEBPA genes within our mixed panel, including a novel SNP in the latter. Three of these nine, along with seven other SNPs selected from the Single Nucleotide Polymorphism database (SNPdb), including SNPs in the RXRA gene, were validated in the crossbred population (N = 260). After validation, five of these SNPs were evaluated for genotype effects on fatty acid content and composition. Significant effects were observed on backfat thickness and different fatty acid contents (P < 0.05). Some of these SNPs caused slight differences in mRNA structure stability and/or putative binding sites for proteins. Conclusions: PPARG and CEBPA showed low to moderate variability in our sample panel. Variations in these genes, along with RXRA, may explain part of the genetic variation in fat content and composition. Our results may contribute to knowledge about genetic variation in meat quality traits in cattle and should be evaluated in larger independent populations.Instituto de Genética Veterinaria2016-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf1-9http://sedici.unlp.edu.ar/handle/10915/100993enginfo:eu-repo/semantics/altIdentifier/url/https://ri.conicet.gov.ar/11336/48668info:eu-repo/semantics/altIdentifier/issn/2055-0391info:eu-repo/semantics/altIdentifier/doi/10.1186/s40781-016-0095-3info:eu-repo/semantics/altIdentifier/hdl/11336/48668info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:20:05Zoai:sedici.unlp.edu.ar:10915/100993Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:20:06.113SEDICI (UNLP) - Universidad Nacional de La Platafalse |
dc.title.none.fl_str_mv |
Genetic characterisation of PPARG, CEBPA and RXRA, and their influence on meat quality traits in cattle |
title |
Genetic characterisation of PPARG, CEBPA and RXRA, and their influence on meat quality traits in cattle |
spellingShingle |
Genetic characterisation of PPARG, CEBPA and RXRA, and their influence on meat quality traits in cattle Goszczynski, Daniel Estanislao Ciencias Veterinarias Polymorphism Variation Association Cows Beef Snp |
title_short |
Genetic characterisation of PPARG, CEBPA and RXRA, and their influence on meat quality traits in cattle |
title_full |
Genetic characterisation of PPARG, CEBPA and RXRA, and their influence on meat quality traits in cattle |
title_fullStr |
Genetic characterisation of PPARG, CEBPA and RXRA, and their influence on meat quality traits in cattle |
title_full_unstemmed |
Genetic characterisation of PPARG, CEBPA and RXRA, and their influence on meat quality traits in cattle |
title_sort |
Genetic characterisation of PPARG, CEBPA and RXRA, and their influence on meat quality traits in cattle |
dc.creator.none.fl_str_mv |
Goszczynski, Daniel Estanislao Papaleo Mazzucco, Juliana Ripoli, María Verónica Villarreal, Edgardo Leopoldo Rogberg Muñoz, Andrés Mezzadra, Carlos Alberto Melucci, Lilia Magdalena Giovambattista, Guillermo |
author |
Goszczynski, Daniel Estanislao |
author_facet |
Goszczynski, Daniel Estanislao Papaleo Mazzucco, Juliana Ripoli, María Verónica Villarreal, Edgardo Leopoldo Rogberg Muñoz, Andrés Mezzadra, Carlos Alberto Melucci, Lilia Magdalena Giovambattista, Guillermo |
author_role |
author |
author2 |
Papaleo Mazzucco, Juliana Ripoli, María Verónica Villarreal, Edgardo Leopoldo Rogberg Muñoz, Andrés Mezzadra, Carlos Alberto Melucci, Lilia Magdalena Giovambattista, Guillermo |
author2_role |
author author author author author author author |
dc.subject.none.fl_str_mv |
Ciencias Veterinarias Polymorphism Variation Association Cows Beef Snp |
topic |
Ciencias Veterinarias Polymorphism Variation Association Cows Beef Snp |
dc.description.none.fl_txt_mv |
Background: Peroxisome proliferator-activated receptor gamma (PPARG), CCAAT/enhancer binding protein alpha (CEBPA) and retinoid X receptor alpha (RXRA) are nuclear transcription factors that play important roles in regulation of adipogenesis and fat deposition. The objectives of this study were to characterise the variability of these three candidate genes in a mixed sample panel composed of several cattle breeds with different meat quality, validate single nucleotide polymorphisms (SNPs) in a local crossbred population (Angus - Hereford - Limousin) and evaluate their effects on meat quality traits (backfat thickness, intramuscular fat content and fatty acid composition), supporting the association tests with bioinformatic predictive studies. Results: Globally, nine SNPs were detected in the PPARG and CEBPA genes within our mixed panel, including a novel SNP in the latter. Three of these nine, along with seven other SNPs selected from the Single Nucleotide Polymorphism database (SNPdb), including SNPs in the RXRA gene, were validated in the crossbred population (N = 260). After validation, five of these SNPs were evaluated for genotype effects on fatty acid content and composition. Significant effects were observed on backfat thickness and different fatty acid contents (P < 0.05). Some of these SNPs caused slight differences in mRNA structure stability and/or putative binding sites for proteins. Conclusions: PPARG and CEBPA showed low to moderate variability in our sample panel. Variations in these genes, along with RXRA, may explain part of the genetic variation in fat content and composition. Our results may contribute to knowledge about genetic variation in meat quality traits in cattle and should be evaluated in larger independent populations. Instituto de Genética Veterinaria |
description |
Background: Peroxisome proliferator-activated receptor gamma (PPARG), CCAAT/enhancer binding protein alpha (CEBPA) and retinoid X receptor alpha (RXRA) are nuclear transcription factors that play important roles in regulation of adipogenesis and fat deposition. The objectives of this study were to characterise the variability of these three candidate genes in a mixed sample panel composed of several cattle breeds with different meat quality, validate single nucleotide polymorphisms (SNPs) in a local crossbred population (Angus - Hereford - Limousin) and evaluate their effects on meat quality traits (backfat thickness, intramuscular fat content and fatty acid composition), supporting the association tests with bioinformatic predictive studies. Results: Globally, nine SNPs were detected in the PPARG and CEBPA genes within our mixed panel, including a novel SNP in the latter. Three of these nine, along with seven other SNPs selected from the Single Nucleotide Polymorphism database (SNPdb), including SNPs in the RXRA gene, were validated in the crossbred population (N = 260). After validation, five of these SNPs were evaluated for genotype effects on fatty acid content and composition. Significant effects were observed on backfat thickness and different fatty acid contents (P < 0.05). Some of these SNPs caused slight differences in mRNA structure stability and/or putative binding sites for proteins. Conclusions: PPARG and CEBPA showed low to moderate variability in our sample panel. Variations in these genes, along with RXRA, may explain part of the genetic variation in fat content and composition. Our results may contribute to knowledge about genetic variation in meat quality traits in cattle and should be evaluated in larger independent populations. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-03 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Articulo http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
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article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://sedici.unlp.edu.ar/handle/10915/100993 |
url |
http://sedici.unlp.edu.ar/handle/10915/100993 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://ri.conicet.gov.ar/11336/48668 info:eu-repo/semantics/altIdentifier/issn/2055-0391 info:eu-repo/semantics/altIdentifier/doi/10.1186/s40781-016-0095-3 info:eu-repo/semantics/altIdentifier/hdl/11336/48668 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
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openAccess |
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http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
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