Genetic Variation in FABP4 and Evaluation of Its Effects on Beef Cattle Fat Content

Autores
Goszczynski, Daniel Estanislao; Papaleo Mazzucco, Juliana; Ripoli, María Verónica; Villarreal, Edgardo Leopoldo; Rogberg Muñoz, Andres; Mezzadra, Carlos Alberto; Melucci, Lilia Magdalena; Giovambattista, Guillermo
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
FABP4 is a protein primarily expressed in adipocytes and macrophages that plays a key role in fatty acid trafficking and lipid hydrolysis. FABP4 gene polymorphisms have been associated with meat quality traits in cattle, mostly in Asian breeds under feedlot conditions. The objectives of this work were to characterize FABP4 genetic variation in several worldwide cattle breeds and evaluate possible genotype effects on fat content in a pasture-fed crossbred (Angus-Hereford-Limousin) population. We re-sequenced 43 unrelated animals from nine cattle breeds (Angus, Brahman, Creole, Hereford, Holstein, Limousin, Nelore, Shorthorn, and Wagyu) and obtained 22 single nucleotide polymorphisms (SNPs) over 3,164 bp, including four novel polymorphisms. Haplotypes and linkage disequilibrium analyses showed a high variability. Five SNPs were selected to perform validation and association studies in our crossbred population. Four SNPs showed well-balanced allele frequencies (minor frequency > 0.159), and three showed no significant deviations from Hardy-Weinberg proportions. SNPs showed significant effects on backfat thickness and fatty acid composition (P < 0.05). The protein structure of one of the missense SNPs was analyzed to elucidate its possible effect on fat content in our studied population. Our results revealed a possible blockage of the fatty acid binding site by the missense mutation.
EEA Balcarce
Fil: Goszczynski, Daniel Estanislao. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina
Fil: Papaleo Mazzucco, Juliana. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Ripoli, María Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina
Fil: Villarreal, Edgardo Leopoldo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Rogberg Muñoz, Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Instituto de Genética Veterinaria; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Fil: Mezzadra, Carlos Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Melucci, Lilia Magdalena. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Giovambattista, Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Instituto de Genética Veterinaria; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Fuente
Animal Biotechnology 28 (3) : 2-9
Materia
Variación Genética
Genetic Variation
Beef Cattle
Lipid Content
Ganado de Carne
Contenido de Lípidos
Gen FABP4
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/617
Acceder
id INTADig_6dd323f7c7f8c4b8260c9b2fa9d3d519
oai_identifier_str oai:localhost:20.500.12123/617
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Genetic Variation in FABP4 and Evaluation of Its Effects on Beef Cattle Fat ContentGoszczynski, Daniel EstanislaoPapaleo Mazzucco, JulianaRipoli, María VerónicaVillarreal, Edgardo LeopoldoRogberg Muñoz, AndresMezzadra, Carlos AlbertoMelucci, Lilia MagdalenaGiovambattista, GuillermoVariación GenéticaGenetic VariationBeef CattleLipid ContentGanado de CarneContenido de LípidosGen FABP4FABP4 is a protein primarily expressed in adipocytes and macrophages that plays a key role in fatty acid trafficking and lipid hydrolysis. FABP4 gene polymorphisms have been associated with meat quality traits in cattle, mostly in Asian breeds under feedlot conditions. The objectives of this work were to characterize FABP4 genetic variation in several worldwide cattle breeds and evaluate possible genotype effects on fat content in a pasture-fed crossbred (Angus-Hereford-Limousin) population. We re-sequenced 43 unrelated animals from nine cattle breeds (Angus, Brahman, Creole, Hereford, Holstein, Limousin, Nelore, Shorthorn, and Wagyu) and obtained 22 single nucleotide polymorphisms (SNPs) over 3,164 bp, including four novel polymorphisms. Haplotypes and linkage disequilibrium analyses showed a high variability. Five SNPs were selected to perform validation and association studies in our crossbred population. Four SNPs showed well-balanced allele frequencies (minor frequency > 0.159), and three showed no significant deviations from Hardy-Weinberg proportions. SNPs showed significant effects on backfat thickness and fatty acid composition (P < 0.05). The protein structure of one of the missense SNPs was analyzed to elucidate its possible effect on fat content in our studied population. Our results revealed a possible blockage of the fatty acid binding site by the missense mutation.EEA BalcarceFil: Goszczynski, Daniel Estanislao. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; ArgentinaFil: Papaleo Mazzucco, Juliana. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; ArgentinaFil: Ripoli, María Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; ArgentinaFil: Villarreal, Edgardo Leopoldo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; ArgentinaFil: Rogberg Muñoz, Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Instituto de Genética Veterinaria; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; ArgentinaFil: Mezzadra, Carlos Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; ArgentinaFil: Melucci, Lilia Magdalena. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; ArgentinaFil: Giovambattista, Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Instituto de Genética Veterinaria; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina2017-07-10T12:30:06Z2017-07-10T12:30:06Z2017-01-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/617http://www.tandfonline.com/doi/abs/10.1080/10495398.2016.1262868?journalCode=labt20Daniel E. Goszczynski, Juliana Papaleo-Mazzucco, María V. Ripoli, Edgardo L. Villarreal, Andrés Rogberg-Muñoz, Carlos A. Mezzadra, Lilia M. Melucci & Guillermo Giovambattista (2017): Genetic Variation in FABP4 and Evaluation of Its Effects on Beef Cattle Fat Content, Animal Biotechnology, DOI: 10.1080/10495398.2016.12628681532-2378http://dx.doi.org/10.1080/10495398.2016.1262868Animal Biotechnology 28 (3) : 2-9reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-29T13:44:06Zoai:localhost:20.500.12123/617instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:44:07.12INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Genetic Variation in FABP4 and Evaluation of Its Effects on Beef Cattle Fat Content
title Genetic Variation in FABP4 and Evaluation of Its Effects on Beef Cattle Fat Content
spellingShingle Genetic Variation in FABP4 and Evaluation of Its Effects on Beef Cattle Fat Content
Goszczynski, Daniel Estanislao
Variación Genética
Genetic Variation
Beef Cattle
Lipid Content
Ganado de Carne
Contenido de Lípidos
Gen FABP4
title_short Genetic Variation in FABP4 and Evaluation of Its Effects on Beef Cattle Fat Content
title_full Genetic Variation in FABP4 and Evaluation of Its Effects on Beef Cattle Fat Content
title_fullStr Genetic Variation in FABP4 and Evaluation of Its Effects on Beef Cattle Fat Content
title_full_unstemmed Genetic Variation in FABP4 and Evaluation of Its Effects on Beef Cattle Fat Content
title_sort Genetic Variation in FABP4 and Evaluation of Its Effects on Beef Cattle Fat Content
dc.creator.none.fl_str_mv Goszczynski, Daniel Estanislao
Papaleo Mazzucco, Juliana
Ripoli, María Verónica
Villarreal, Edgardo Leopoldo
Rogberg Muñoz, Andres
Mezzadra, Carlos Alberto
Melucci, Lilia Magdalena
Giovambattista, Guillermo
author Goszczynski, Daniel Estanislao
author_facet Goszczynski, Daniel Estanislao
Papaleo Mazzucco, Juliana
Ripoli, María Verónica
Villarreal, Edgardo Leopoldo
Rogberg Muñoz, Andres
Mezzadra, Carlos Alberto
Melucci, Lilia Magdalena
Giovambattista, Guillermo
author_role author
author2 Papaleo Mazzucco, Juliana
Ripoli, María Verónica
Villarreal, Edgardo Leopoldo
Rogberg Muñoz, Andres
Mezzadra, Carlos Alberto
Melucci, Lilia Magdalena
Giovambattista, Guillermo
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Variación Genética
Genetic Variation
Beef Cattle
Lipid Content
Ganado de Carne
Contenido de Lípidos
Gen FABP4
topic Variación Genética
Genetic Variation
Beef Cattle
Lipid Content
Ganado de Carne
Contenido de Lípidos
Gen FABP4
dc.description.none.fl_txt_mv FABP4 is a protein primarily expressed in adipocytes and macrophages that plays a key role in fatty acid trafficking and lipid hydrolysis. FABP4 gene polymorphisms have been associated with meat quality traits in cattle, mostly in Asian breeds under feedlot conditions. The objectives of this work were to characterize FABP4 genetic variation in several worldwide cattle breeds and evaluate possible genotype effects on fat content in a pasture-fed crossbred (Angus-Hereford-Limousin) population. We re-sequenced 43 unrelated animals from nine cattle breeds (Angus, Brahman, Creole, Hereford, Holstein, Limousin, Nelore, Shorthorn, and Wagyu) and obtained 22 single nucleotide polymorphisms (SNPs) over 3,164 bp, including four novel polymorphisms. Haplotypes and linkage disequilibrium analyses showed a high variability. Five SNPs were selected to perform validation and association studies in our crossbred population. Four SNPs showed well-balanced allele frequencies (minor frequency > 0.159), and three showed no significant deviations from Hardy-Weinberg proportions. SNPs showed significant effects on backfat thickness and fatty acid composition (P < 0.05). The protein structure of one of the missense SNPs was analyzed to elucidate its possible effect on fat content in our studied population. Our results revealed a possible blockage of the fatty acid binding site by the missense mutation.
EEA Balcarce
Fil: Goszczynski, Daniel Estanislao. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina
Fil: Papaleo Mazzucco, Juliana. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Ripoli, María Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina
Fil: Villarreal, Edgardo Leopoldo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Rogberg Muñoz, Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Instituto de Genética Veterinaria; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Fil: Mezzadra, Carlos Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Melucci, Lilia Magdalena. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Giovambattista, Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Instituto de Genética Veterinaria; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
description FABP4 is a protein primarily expressed in adipocytes and macrophages that plays a key role in fatty acid trafficking and lipid hydrolysis. FABP4 gene polymorphisms have been associated with meat quality traits in cattle, mostly in Asian breeds under feedlot conditions. The objectives of this work were to characterize FABP4 genetic variation in several worldwide cattle breeds and evaluate possible genotype effects on fat content in a pasture-fed crossbred (Angus-Hereford-Limousin) population. We re-sequenced 43 unrelated animals from nine cattle breeds (Angus, Brahman, Creole, Hereford, Holstein, Limousin, Nelore, Shorthorn, and Wagyu) and obtained 22 single nucleotide polymorphisms (SNPs) over 3,164 bp, including four novel polymorphisms. Haplotypes and linkage disequilibrium analyses showed a high variability. Five SNPs were selected to perform validation and association studies in our crossbred population. Four SNPs showed well-balanced allele frequencies (minor frequency > 0.159), and three showed no significant deviations from Hardy-Weinberg proportions. SNPs showed significant effects on backfat thickness and fatty acid composition (P < 0.05). The protein structure of one of the missense SNPs was analyzed to elucidate its possible effect on fat content in our studied population. Our results revealed a possible blockage of the fatty acid binding site by the missense mutation.
publishDate 2017
dc.date.none.fl_str_mv 2017-07-10T12:30:06Z
2017-07-10T12:30:06Z
2017-01-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/617
http://www.tandfonline.com/doi/abs/10.1080/10495398.2016.1262868?journalCode=labt20
Daniel E. Goszczynski, Juliana Papaleo-Mazzucco, María V. Ripoli, Edgardo L. Villarreal, Andrés Rogberg-Muñoz, Carlos A. Mezzadra, Lilia M. Melucci & Guillermo Giovambattista (2017): Genetic Variation in FABP4 and Evaluation of Its Effects on Beef Cattle Fat Content, Animal Biotechnology, DOI: 10.1080/10495398.2016.1262868
1532-2378
http://dx.doi.org/10.1080/10495398.2016.1262868
url http://hdl.handle.net/20.500.12123/617
http://www.tandfonline.com/doi/abs/10.1080/10495398.2016.1262868?journalCode=labt20
http://dx.doi.org/10.1080/10495398.2016.1262868
identifier_str_mv Daniel E. Goszczynski, Juliana Papaleo-Mazzucco, María V. Ripoli, Edgardo L. Villarreal, Andrés Rogberg-Muñoz, Carlos A. Mezzadra, Lilia M. Melucci & Guillermo Giovambattista (2017): Genetic Variation in FABP4 and Evaluation of Its Effects on Beef Cattle Fat Content, Animal Biotechnology, DOI: 10.1080/10495398.2016.1262868
1532-2378
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Animal Biotechnology 28 (3) : 2-9
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
_version_ 1844619115058167808
score 12.558318

Publicaciones similares