Study on the composition-structure relationship of Iipophorins

Autores
Soulages, José Luis; Brenner, Rodolfo Roberto
Año de publicación
1991
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
High density lipophorin (HDLp is the main lipoprotein found in resting insect hemolymph. It has, in general, two molecules of apolipoproteins: apoLp-I (250 kDa) and apoLp-II (80 kDa) and a variable lipid content which ranges from 35% to 59% (w/w). Diacylglycerols (DG), phospholipids (PL), and hydrocarbons (HC) are the main lipid components, whereas cholesterol and triacylglycerols are minor components. DG content varies from 7 to 30%, PL from 11 to 24%, and HC from 0 to 15%. In order to determine the relationship between the lipid composition and the arrangement of lipid and protein components in the lipoprotein particle, a density-composition structural model was designed. The model was established by means of 12 sets of data on lipophorin density-composition relationships, and model validity was determined throughout lipoprotein space- and surface-filling conditions. Despite the differences among the lipid compositions of lipophorins, it is concluded that there are several unifying structural restrictions that govern the molecular organization of lipophorins. Quantitative treatment of the model indicates that lipophorin structure is consistent with the following. 1) Spherical particles with a protein-rich outer layer of approximately 20-21 Å thickness, comprised of proteins, phospholipids, cholesterol, and small amounts of DG, and a lipid-rich core composed of HC, TG, and almost all the lipophorin DG. 2) Apolipophorins have a lipid-embedded localization within the lipoprotein particle. They might represent one of the few examples of proteins containing beta-shift structure, exerting strong hydrophobic interaction and having a lipid-embedded localization. 3) On the lipophorin surface, apoproteins occupy about 31 x 10³ Ų that represents from 62 to 82% of the total lipoprotein surface, depending on the size of lipophorin considered. 4) The content of PL of each lipophorin is closely proportional to the lipoprotein size. 5) Despite the importance of lipophorin as DG carrier, there are only small amounts of this component on the lipophorin surface which could reach a maximal surface lipid concentration of about 10 mol%.
Instituto de Investigaciones Bioquímicas de La Plata
Materia
Química
Lipophorin model.
Lipoprotein structure
Insect lipoprotein
Lipid transport
Lipid-protein interaction
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/120454

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oai_identifier_str oai:sedici.unlp.edu.ar:10915/120454
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Study on the composition-structure relationship of IipophorinsSoulages, José LuisBrenner, Rodolfo RobertoQuímicaLipophorin model.Lipoprotein structureInsect lipoproteinLipid transportLipid-protein interactionHigh density lipophorin (HDLp is the main lipoprotein found in resting insect hemolymph. It has, in general, two molecules of apolipoproteins: apoLp-I (250 kDa) and apoLp-II (80 kDa) and a variable lipid content which ranges from 35% to 59% (w/w). Diacylglycerols (DG), phospholipids (PL), and hydrocarbons (HC) are the main lipid components, whereas cholesterol and triacylglycerols are minor components. DG content varies from 7 to 30%, PL from 11 to 24%, and HC from 0 to 15%. In order to determine the relationship between the lipid composition and the arrangement of lipid and protein components in the lipoprotein particle, a density-composition structural model was designed. The model was established by means of 12 sets of data on lipophorin density-composition relationships, and model validity was determined throughout lipoprotein space- and surface-filling conditions. Despite the differences among the lipid compositions of lipophorins, it is concluded that there are several unifying structural restrictions that govern the molecular organization of lipophorins. Quantitative treatment of the model indicates that lipophorin structure is consistent with the following. 1) Spherical particles with a protein-rich outer layer of approximately 20-21 Å thickness, comprised of proteins, phospholipids, cholesterol, and small amounts of DG, and a lipid-rich core composed of HC, TG, and almost all the lipophorin DG. 2) Apolipophorins have a lipid-embedded localization within the lipoprotein particle. They might represent one of the few examples of proteins containing beta-shift structure, exerting strong hydrophobic interaction and having a lipid-embedded localization. 3) On the lipophorin surface, apoproteins occupy about 31 x 10³ Ų that represents from 62 to 82% of the total lipoprotein surface, depending on the size of lipophorin considered. 4) The content of PL of each lipophorin is closely proportional to the lipoprotein size. 5) Despite the importance of lipophorin as DG carrier, there are only small amounts of this component on the lipophorin surface which could reach a maximal surface lipid concentration of about 10 mol%.Instituto de Investigaciones Bioquímicas de La Plata1991info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf407-415http://sedici.unlp.edu.ar/handle/10915/120454enginfo:eu-repo/semantics/altIdentifier/issn/0022-2275info:eu-repo/semantics/altIdentifier/doi/10.1016/S0022-2275(20)42063-2info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:28:30Zoai:sedici.unlp.edu.ar:10915/120454Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:28:30.602SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Study on the composition-structure relationship of Iipophorins
title Study on the composition-structure relationship of Iipophorins
spellingShingle Study on the composition-structure relationship of Iipophorins
Soulages, José Luis
Química
Lipophorin model.
Lipoprotein structure
Insect lipoprotein
Lipid transport
Lipid-protein interaction
title_short Study on the composition-structure relationship of Iipophorins
title_full Study on the composition-structure relationship of Iipophorins
title_fullStr Study on the composition-structure relationship of Iipophorins
title_full_unstemmed Study on the composition-structure relationship of Iipophorins
title_sort Study on the composition-structure relationship of Iipophorins
dc.creator.none.fl_str_mv Soulages, José Luis
Brenner, Rodolfo Roberto
author Soulages, José Luis
author_facet Soulages, José Luis
Brenner, Rodolfo Roberto
author_role author
author2 Brenner, Rodolfo Roberto
author2_role author
dc.subject.none.fl_str_mv Química
Lipophorin model.
Lipoprotein structure
Insect lipoprotein
Lipid transport
Lipid-protein interaction
topic Química
Lipophorin model.
Lipoprotein structure
Insect lipoprotein
Lipid transport
Lipid-protein interaction
dc.description.none.fl_txt_mv High density lipophorin (HDLp is the main lipoprotein found in resting insect hemolymph. It has, in general, two molecules of apolipoproteins: apoLp-I (250 kDa) and apoLp-II (80 kDa) and a variable lipid content which ranges from 35% to 59% (w/w). Diacylglycerols (DG), phospholipids (PL), and hydrocarbons (HC) are the main lipid components, whereas cholesterol and triacylglycerols are minor components. DG content varies from 7 to 30%, PL from 11 to 24%, and HC from 0 to 15%. In order to determine the relationship between the lipid composition and the arrangement of lipid and protein components in the lipoprotein particle, a density-composition structural model was designed. The model was established by means of 12 sets of data on lipophorin density-composition relationships, and model validity was determined throughout lipoprotein space- and surface-filling conditions. Despite the differences among the lipid compositions of lipophorins, it is concluded that there are several unifying structural restrictions that govern the molecular organization of lipophorins. Quantitative treatment of the model indicates that lipophorin structure is consistent with the following. 1) Spherical particles with a protein-rich outer layer of approximately 20-21 Å thickness, comprised of proteins, phospholipids, cholesterol, and small amounts of DG, and a lipid-rich core composed of HC, TG, and almost all the lipophorin DG. 2) Apolipophorins have a lipid-embedded localization within the lipoprotein particle. They might represent one of the few examples of proteins containing beta-shift structure, exerting strong hydrophobic interaction and having a lipid-embedded localization. 3) On the lipophorin surface, apoproteins occupy about 31 x 10³ Ų that represents from 62 to 82% of the total lipoprotein surface, depending on the size of lipophorin considered. 4) The content of PL of each lipophorin is closely proportional to the lipoprotein size. 5) Despite the importance of lipophorin as DG carrier, there are only small amounts of this component on the lipophorin surface which could reach a maximal surface lipid concentration of about 10 mol%.
Instituto de Investigaciones Bioquímicas de La Plata
description High density lipophorin (HDLp is the main lipoprotein found in resting insect hemolymph. It has, in general, two molecules of apolipoproteins: apoLp-I (250 kDa) and apoLp-II (80 kDa) and a variable lipid content which ranges from 35% to 59% (w/w). Diacylglycerols (DG), phospholipids (PL), and hydrocarbons (HC) are the main lipid components, whereas cholesterol and triacylglycerols are minor components. DG content varies from 7 to 30%, PL from 11 to 24%, and HC from 0 to 15%. In order to determine the relationship between the lipid composition and the arrangement of lipid and protein components in the lipoprotein particle, a density-composition structural model was designed. The model was established by means of 12 sets of data on lipophorin density-composition relationships, and model validity was determined throughout lipoprotein space- and surface-filling conditions. Despite the differences among the lipid compositions of lipophorins, it is concluded that there are several unifying structural restrictions that govern the molecular organization of lipophorins. Quantitative treatment of the model indicates that lipophorin structure is consistent with the following. 1) Spherical particles with a protein-rich outer layer of approximately 20-21 Å thickness, comprised of proteins, phospholipids, cholesterol, and small amounts of DG, and a lipid-rich core composed of HC, TG, and almost all the lipophorin DG. 2) Apolipophorins have a lipid-embedded localization within the lipoprotein particle. They might represent one of the few examples of proteins containing beta-shift structure, exerting strong hydrophobic interaction and having a lipid-embedded localization. 3) On the lipophorin surface, apoproteins occupy about 31 x 10³ Ų that represents from 62 to 82% of the total lipoprotein surface, depending on the size of lipophorin considered. 4) The content of PL of each lipophorin is closely proportional to the lipoprotein size. 5) Despite the importance of lipophorin as DG carrier, there are only small amounts of this component on the lipophorin surface which could reach a maximal surface lipid concentration of about 10 mol%.
publishDate 1991
dc.date.none.fl_str_mv 1991
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/120454
url http://sedici.unlp.edu.ar/handle/10915/120454
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/0022-2275
info:eu-repo/semantics/altIdentifier/doi/10.1016/S0022-2275(20)42063-2
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
dc.format.none.fl_str_mv application/pdf
407-415
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repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
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