Study on the composition-structure relationship of Iipophorins
- Autores
- Soulages, José Luis; Brenner, Rodolfo Roberto
- Año de publicación
- 1991
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- High density lipophorin (HDLp is the main lipoprotein found in resting insect hemolymph. It has, in general, two molecules of apolipoproteins: apoLp-I (250 kDa) and apoLp-II (80 kDa) and a variable lipid content which ranges from 35% to 59% (w/w). Diacylglycerols (DG), phospholipids (PL), and hydrocarbons (HC) are the main lipid components, whereas cholesterol and triacylglycerols are minor components. DG content varies from 7 to 30%, PL from 11 to 24%, and HC from 0 to 15%. In order to determine the relationship between the lipid composition and the arrangement of lipid and protein components in the lipoprotein particle, a density-composition structural model was designed. The model was established by means of 12 sets of data on lipophorin density-composition relationships, and model validity was determined throughout lipoprotein space- and surface-filling conditions. Despite the differences among the lipid compositions of lipophorins, it is concluded that there are several unifying structural restrictions that govern the molecular organization of lipophorins. Quantitative treatment of the model indicates that lipophorin structure is consistent with the following. 1) Spherical particles with a protein-rich outer layer of approximately 20-21 Å thickness, comprised of proteins, phospholipids, cholesterol, and small amounts of DG, and a lipid-rich core composed of HC, TG, and almost all the lipophorin DG. 2) Apolipophorins have a lipid-embedded localization within the lipoprotein particle. They might represent one of the few examples of proteins containing beta-shift structure, exerting strong hydrophobic interaction and having a lipid-embedded localization. 3) On the lipophorin surface, apoproteins occupy about 31 x 10³ Ų that represents from 62 to 82% of the total lipoprotein surface, depending on the size of lipophorin considered. 4) The content of PL of each lipophorin is closely proportional to the lipoprotein size. 5) Despite the importance of lipophorin as DG carrier, there are only small amounts of this component on the lipophorin surface which could reach a maximal surface lipid concentration of about 10 mol%.
Instituto de Investigaciones Bioquímicas de La Plata - Materia
-
Química
Lipophorin model.
Lipoprotein structure
Insect lipoprotein
Lipid transport
Lipid-protein interaction - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by/4.0/
- Repositorio
- Institución
- Universidad Nacional de La Plata
- OAI Identificador
- oai:sedici.unlp.edu.ar:10915/120454
Ver los metadatos del registro completo
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Study on the composition-structure relationship of IipophorinsSoulages, José LuisBrenner, Rodolfo RobertoQuímicaLipophorin model.Lipoprotein structureInsect lipoproteinLipid transportLipid-protein interactionHigh density lipophorin (HDLp is the main lipoprotein found in resting insect hemolymph. It has, in general, two molecules of apolipoproteins: apoLp-I (250 kDa) and apoLp-II (80 kDa) and a variable lipid content which ranges from 35% to 59% (w/w). Diacylglycerols (DG), phospholipids (PL), and hydrocarbons (HC) are the main lipid components, whereas cholesterol and triacylglycerols are minor components. DG content varies from 7 to 30%, PL from 11 to 24%, and HC from 0 to 15%. In order to determine the relationship between the lipid composition and the arrangement of lipid and protein components in the lipoprotein particle, a density-composition structural model was designed. The model was established by means of 12 sets of data on lipophorin density-composition relationships, and model validity was determined throughout lipoprotein space- and surface-filling conditions. Despite the differences among the lipid compositions of lipophorins, it is concluded that there are several unifying structural restrictions that govern the molecular organization of lipophorins. Quantitative treatment of the model indicates that lipophorin structure is consistent with the following. 1) Spherical particles with a protein-rich outer layer of approximately 20-21 Å thickness, comprised of proteins, phospholipids, cholesterol, and small amounts of DG, and a lipid-rich core composed of HC, TG, and almost all the lipophorin DG. 2) Apolipophorins have a lipid-embedded localization within the lipoprotein particle. They might represent one of the few examples of proteins containing beta-shift structure, exerting strong hydrophobic interaction and having a lipid-embedded localization. 3) On the lipophorin surface, apoproteins occupy about 31 x 10³ Ų that represents from 62 to 82% of the total lipoprotein surface, depending on the size of lipophorin considered. 4) The content of PL of each lipophorin is closely proportional to the lipoprotein size. 5) Despite the importance of lipophorin as DG carrier, there are only small amounts of this component on the lipophorin surface which could reach a maximal surface lipid concentration of about 10 mol%.Instituto de Investigaciones Bioquímicas de La Plata1991info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf407-415http://sedici.unlp.edu.ar/handle/10915/120454enginfo:eu-repo/semantics/altIdentifier/issn/0022-2275info:eu-repo/semantics/altIdentifier/doi/10.1016/S0022-2275(20)42063-2info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:28:30Zoai:sedici.unlp.edu.ar:10915/120454Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:28:30.602SEDICI (UNLP) - Universidad Nacional de La Platafalse |
dc.title.none.fl_str_mv |
Study on the composition-structure relationship of Iipophorins |
title |
Study on the composition-structure relationship of Iipophorins |
spellingShingle |
Study on the composition-structure relationship of Iipophorins Soulages, José Luis Química Lipophorin model. Lipoprotein structure Insect lipoprotein Lipid transport Lipid-protein interaction |
title_short |
Study on the composition-structure relationship of Iipophorins |
title_full |
Study on the composition-structure relationship of Iipophorins |
title_fullStr |
Study on the composition-structure relationship of Iipophorins |
title_full_unstemmed |
Study on the composition-structure relationship of Iipophorins |
title_sort |
Study on the composition-structure relationship of Iipophorins |
dc.creator.none.fl_str_mv |
Soulages, José Luis Brenner, Rodolfo Roberto |
author |
Soulages, José Luis |
author_facet |
Soulages, José Luis Brenner, Rodolfo Roberto |
author_role |
author |
author2 |
Brenner, Rodolfo Roberto |
author2_role |
author |
dc.subject.none.fl_str_mv |
Química Lipophorin model. Lipoprotein structure Insect lipoprotein Lipid transport Lipid-protein interaction |
topic |
Química Lipophorin model. Lipoprotein structure Insect lipoprotein Lipid transport Lipid-protein interaction |
dc.description.none.fl_txt_mv |
High density lipophorin (HDLp is the main lipoprotein found in resting insect hemolymph. It has, in general, two molecules of apolipoproteins: apoLp-I (250 kDa) and apoLp-II (80 kDa) and a variable lipid content which ranges from 35% to 59% (w/w). Diacylglycerols (DG), phospholipids (PL), and hydrocarbons (HC) are the main lipid components, whereas cholesterol and triacylglycerols are minor components. DG content varies from 7 to 30%, PL from 11 to 24%, and HC from 0 to 15%. In order to determine the relationship between the lipid composition and the arrangement of lipid and protein components in the lipoprotein particle, a density-composition structural model was designed. The model was established by means of 12 sets of data on lipophorin density-composition relationships, and model validity was determined throughout lipoprotein space- and surface-filling conditions. Despite the differences among the lipid compositions of lipophorins, it is concluded that there are several unifying structural restrictions that govern the molecular organization of lipophorins. Quantitative treatment of the model indicates that lipophorin structure is consistent with the following. 1) Spherical particles with a protein-rich outer layer of approximately 20-21 Å thickness, comprised of proteins, phospholipids, cholesterol, and small amounts of DG, and a lipid-rich core composed of HC, TG, and almost all the lipophorin DG. 2) Apolipophorins have a lipid-embedded localization within the lipoprotein particle. They might represent one of the few examples of proteins containing beta-shift structure, exerting strong hydrophobic interaction and having a lipid-embedded localization. 3) On the lipophorin surface, apoproteins occupy about 31 x 10³ Ų that represents from 62 to 82% of the total lipoprotein surface, depending on the size of lipophorin considered. 4) The content of PL of each lipophorin is closely proportional to the lipoprotein size. 5) Despite the importance of lipophorin as DG carrier, there are only small amounts of this component on the lipophorin surface which could reach a maximal surface lipid concentration of about 10 mol%. Instituto de Investigaciones Bioquímicas de La Plata |
description |
High density lipophorin (HDLp is the main lipoprotein found in resting insect hemolymph. It has, in general, two molecules of apolipoproteins: apoLp-I (250 kDa) and apoLp-II (80 kDa) and a variable lipid content which ranges from 35% to 59% (w/w). Diacylglycerols (DG), phospholipids (PL), and hydrocarbons (HC) are the main lipid components, whereas cholesterol and triacylglycerols are minor components. DG content varies from 7 to 30%, PL from 11 to 24%, and HC from 0 to 15%. In order to determine the relationship between the lipid composition and the arrangement of lipid and protein components in the lipoprotein particle, a density-composition structural model was designed. The model was established by means of 12 sets of data on lipophorin density-composition relationships, and model validity was determined throughout lipoprotein space- and surface-filling conditions. Despite the differences among the lipid compositions of lipophorins, it is concluded that there are several unifying structural restrictions that govern the molecular organization of lipophorins. Quantitative treatment of the model indicates that lipophorin structure is consistent with the following. 1) Spherical particles with a protein-rich outer layer of approximately 20-21 Å thickness, comprised of proteins, phospholipids, cholesterol, and small amounts of DG, and a lipid-rich core composed of HC, TG, and almost all the lipophorin DG. 2) Apolipophorins have a lipid-embedded localization within the lipoprotein particle. They might represent one of the few examples of proteins containing beta-shift structure, exerting strong hydrophobic interaction and having a lipid-embedded localization. 3) On the lipophorin surface, apoproteins occupy about 31 x 10³ Ų that represents from 62 to 82% of the total lipoprotein surface, depending on the size of lipophorin considered. 4) The content of PL of each lipophorin is closely proportional to the lipoprotein size. 5) Despite the importance of lipophorin as DG carrier, there are only small amounts of this component on the lipophorin surface which could reach a maximal surface lipid concentration of about 10 mol%. |
publishDate |
1991 |
dc.date.none.fl_str_mv |
1991 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Articulo http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://sedici.unlp.edu.ar/handle/10915/120454 |
url |
http://sedici.unlp.edu.ar/handle/10915/120454 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/issn/0022-2275 info:eu-repo/semantics/altIdentifier/doi/10.1016/S0022-2275(20)42063-2 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/4.0/ Creative Commons Attribution 4.0 International (CC BY 4.0) |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by/4.0/ Creative Commons Attribution 4.0 International (CC BY 4.0) |
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application/pdf 407-415 |
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