Photoinduced cleavage of plasmid DNA in the presence of pterin

Autores
Llorente, Carlos Luis; Thomas, Andrés Héctor; Villata, Laura Sofía; Hozbor, Daniela Flavia; Lagares, Antonio; Capparelli, Alberto Luis
Año de publicación
2000
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The photoinduced cleavage of plasmid DNA by UV-A light in the presence of pterin was investigated. Electrophoretic analysis of the irradiated plasmid pUC18 in the presence of pterin showed that UV light of 350 nm induced the transformation of a significant proportion of the supercoiled plasmid to its relaxed form. A minor proportion of plasmid forms are also converted to the linear plasmid isomer at the longer irradiation times. All these transformations during irradiation can be observed in the absorption spectrum of DNA as function of time. Such spectral modifications correlated with the extent and the kinetics of plasmid relaxation, but not with the appearance of the linear plasmid. None of these changes were operative without the irradiation with UV-A light. Control experiments with pterin or plasmid DNA irradiated separately, showed no photochemical changes. Results taken together suggest that the observed changes in the supercoiled plasmid as well as the spectral modifications both derive from the generation of single-strand break in the DNA.
Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas
Instituto de Biotecnologia y Biologia Molecular
Materia
Bioquímica
plasmid DNA
UV-A light
pterin
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/83444
Acceder
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oai_identifier_str oai:sedici.unlp.edu.ar:10915/83444
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Photoinduced cleavage of plasmid DNA in the presence of pterinLlorente, Carlos LuisThomas, Andrés HéctorVillata, Laura SofíaHozbor, Daniela FlaviaLagares, AntonioCapparelli, Alberto LuisBioquímicaplasmid DNAUV-A lightpterinThe photoinduced cleavage of plasmid DNA by UV-A light in the presence of pterin was investigated. Electrophoretic analysis of the irradiated plasmid pUC18 in the presence of pterin showed that UV light of 350 nm induced the transformation of a significant proportion of the supercoiled plasmid to its relaxed form. A minor proportion of plasmid forms are also converted to the linear plasmid isomer at the longer irradiation times. All these transformations during irradiation can be observed in the absorption spectrum of DNA as function of time. Such spectral modifications correlated with the extent and the kinetics of plasmid relaxation, but not with the appearance of the linear plasmid. None of these changes were operative without the irradiation with UV-A light. Control experiments with pterin or plasmid DNA irradiated separately, showed no photochemical changes. Results taken together suggest that the observed changes in the supercoiled plasmid as well as the spectral modifications both derive from the generation of single-strand break in the DNA.Instituto de Investigaciones Fisicoquímicas Teóricas y AplicadasInstituto de Biotecnologia y Biologia Molecular2000info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf100-105http://sedici.unlp.edu.ar/handle/10915/83444enginfo:eu-repo/semantics/altIdentifier/issn/0933-4807info:eu-repo/semantics/altIdentifier/doi/10.1515/pteridines.2000.11.3.100info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-11-12T10:40:08Zoai:sedici.unlp.edu.ar:10915/83444Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-11-12 10:40:08.651SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Photoinduced cleavage of plasmid DNA in the presence of pterin
title Photoinduced cleavage of plasmid DNA in the presence of pterin
spellingShingle Photoinduced cleavage of plasmid DNA in the presence of pterin
Llorente, Carlos Luis
Bioquímica
plasmid DNA
UV-A light
pterin
title_short Photoinduced cleavage of plasmid DNA in the presence of pterin
title_full Photoinduced cleavage of plasmid DNA in the presence of pterin
title_fullStr Photoinduced cleavage of plasmid DNA in the presence of pterin
title_full_unstemmed Photoinduced cleavage of plasmid DNA in the presence of pterin
title_sort Photoinduced cleavage of plasmid DNA in the presence of pterin
dc.creator.none.fl_str_mv Llorente, Carlos Luis
Thomas, Andrés Héctor
Villata, Laura Sofía
Hozbor, Daniela Flavia
Lagares, Antonio
Capparelli, Alberto Luis
author Llorente, Carlos Luis
author_facet Llorente, Carlos Luis
Thomas, Andrés Héctor
Villata, Laura Sofía
Hozbor, Daniela Flavia
Lagares, Antonio
Capparelli, Alberto Luis
author_role author
author2 Thomas, Andrés Héctor
Villata, Laura Sofía
Hozbor, Daniela Flavia
Lagares, Antonio
Capparelli, Alberto Luis
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Bioquímica
plasmid DNA
UV-A light
pterin
topic Bioquímica
plasmid DNA
UV-A light
pterin
dc.description.none.fl_txt_mv The photoinduced cleavage of plasmid DNA by UV-A light in the presence of pterin was investigated. Electrophoretic analysis of the irradiated plasmid pUC18 in the presence of pterin showed that UV light of 350 nm induced the transformation of a significant proportion of the supercoiled plasmid to its relaxed form. A minor proportion of plasmid forms are also converted to the linear plasmid isomer at the longer irradiation times. All these transformations during irradiation can be observed in the absorption spectrum of DNA as function of time. Such spectral modifications correlated with the extent and the kinetics of plasmid relaxation, but not with the appearance of the linear plasmid. None of these changes were operative without the irradiation with UV-A light. Control experiments with pterin or plasmid DNA irradiated separately, showed no photochemical changes. Results taken together suggest that the observed changes in the supercoiled plasmid as well as the spectral modifications both derive from the generation of single-strand break in the DNA.
Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas
Instituto de Biotecnologia y Biologia Molecular
description The photoinduced cleavage of plasmid DNA by UV-A light in the presence of pterin was investigated. Electrophoretic analysis of the irradiated plasmid pUC18 in the presence of pterin showed that UV light of 350 nm induced the transformation of a significant proportion of the supercoiled plasmid to its relaxed form. A minor proportion of plasmid forms are also converted to the linear plasmid isomer at the longer irradiation times. All these transformations during irradiation can be observed in the absorption spectrum of DNA as function of time. Such spectral modifications correlated with the extent and the kinetics of plasmid relaxation, but not with the appearance of the linear plasmid. None of these changes were operative without the irradiation with UV-A light. Control experiments with pterin or plasmid DNA irradiated separately, showed no photochemical changes. Results taken together suggest that the observed changes in the supercoiled plasmid as well as the spectral modifications both derive from the generation of single-strand break in the DNA.
publishDate 2000
dc.date.none.fl_str_mv 2000
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/83444
url http://sedici.unlp.edu.ar/handle/10915/83444
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/0933-4807
info:eu-repo/semantics/altIdentifier/doi/10.1515/pteridines.2000.11.3.100
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
100-105
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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score 12.976206

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