Soybean phosphatidylcholine liposomes as model membranes to study lipid peroxidation photoinduced by pterin

Autores
Thomas, Andrés Héctor; Catalá, Ángel; Vignoni, Mariana
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Oxidized pterins, efficient photosensitizers under UVA irradiation, accumulate in the skin of patients suffering from vitiligo, a chronic depigmentation disorder. Soybean phosphatidylcholine (SoyPC) liposomes were employed as model membranes to investigate if pterin (Ptr), the parent compound of oxidized pterins, is able to photoinduced lipid peroxidation. Size exclusion chromatography and dialysis experiments showed that Ptr is not encapsulated inside the liposomes and the lipid membrane is permeable to this compound. The formation of conjugated dienes and trienes, upon UVA irradiation, was followed by absorption at 234 and 270 nm, respectively. The photoproducts were characterized by mass spectrometry and oxygenation of SoyPC was demonstrated. In addition, analysis of MS/MS spectra suggested the formation hydroperoxides. Finally, the biological implications of the findings are discussed.
Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas
Materia
Química
Física
Hydroperoxide
Liposome
Phosphatidylcholine
Pterin
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/86070
Acceder
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oai_identifier_str oai:sedici.unlp.edu.ar:10915/86070
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Soybean phosphatidylcholine liposomes as model membranes to study lipid peroxidation photoinduced by pterinThomas, Andrés HéctorCatalá, ÁngelVignoni, MarianaQuímicaFísicaHydroperoxideLiposomePhosphatidylcholinePterinOxidized pterins, efficient photosensitizers under UVA irradiation, accumulate in the skin of patients suffering from vitiligo, a chronic depigmentation disorder. Soybean phosphatidylcholine (SoyPC) liposomes were employed as model membranes to investigate if pterin (Ptr), the parent compound of oxidized pterins, is able to photoinduced lipid peroxidation. Size exclusion chromatography and dialysis experiments showed that Ptr is not encapsulated inside the liposomes and the lipid membrane is permeable to this compound. The formation of conjugated dienes and trienes, upon UVA irradiation, was followed by absorption at 234 and 270 nm, respectively. The photoproducts were characterized by mass spectrometry and oxygenation of SoyPC was demonstrated. In addition, analysis of MS/MS spectra suggested the formation hydroperoxides. Finally, the biological implications of the findings are discussed.Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas2016info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf139-145http://sedici.unlp.edu.ar/handle/10915/86070enginfo:eu-repo/semantics/altIdentifier/issn/0005-2736info:eu-repo/semantics/altIdentifier/doi/10.1016/j.bbamem.2015.11.002info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-03T10:49:07Zoai:sedici.unlp.edu.ar:10915/86070Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-03 10:49:08.006SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Soybean phosphatidylcholine liposomes as model membranes to study lipid peroxidation photoinduced by pterin
title Soybean phosphatidylcholine liposomes as model membranes to study lipid peroxidation photoinduced by pterin
spellingShingle Soybean phosphatidylcholine liposomes as model membranes to study lipid peroxidation photoinduced by pterin
Thomas, Andrés Héctor
Química
Física
Hydroperoxide
Liposome
Phosphatidylcholine
Pterin
title_short Soybean phosphatidylcholine liposomes as model membranes to study lipid peroxidation photoinduced by pterin
title_full Soybean phosphatidylcholine liposomes as model membranes to study lipid peroxidation photoinduced by pterin
title_fullStr Soybean phosphatidylcholine liposomes as model membranes to study lipid peroxidation photoinduced by pterin
title_full_unstemmed Soybean phosphatidylcholine liposomes as model membranes to study lipid peroxidation photoinduced by pterin
title_sort Soybean phosphatidylcholine liposomes as model membranes to study lipid peroxidation photoinduced by pterin
dc.creator.none.fl_str_mv Thomas, Andrés Héctor
Catalá, Ángel
Vignoni, Mariana
author Thomas, Andrés Héctor
author_facet Thomas, Andrés Héctor
Catalá, Ángel
Vignoni, Mariana
author_role author
author2 Catalá, Ángel
Vignoni, Mariana
author2_role author
author
dc.subject.none.fl_str_mv Química
Física
Hydroperoxide
Liposome
Phosphatidylcholine
Pterin
topic Química
Física
Hydroperoxide
Liposome
Phosphatidylcholine
Pterin
dc.description.none.fl_txt_mv Oxidized pterins, efficient photosensitizers under UVA irradiation, accumulate in the skin of patients suffering from vitiligo, a chronic depigmentation disorder. Soybean phosphatidylcholine (SoyPC) liposomes were employed as model membranes to investigate if pterin (Ptr), the parent compound of oxidized pterins, is able to photoinduced lipid peroxidation. Size exclusion chromatography and dialysis experiments showed that Ptr is not encapsulated inside the liposomes and the lipid membrane is permeable to this compound. The formation of conjugated dienes and trienes, upon UVA irradiation, was followed by absorption at 234 and 270 nm, respectively. The photoproducts were characterized by mass spectrometry and oxygenation of SoyPC was demonstrated. In addition, analysis of MS/MS spectra suggested the formation hydroperoxides. Finally, the biological implications of the findings are discussed.
Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas
description Oxidized pterins, efficient photosensitizers under UVA irradiation, accumulate in the skin of patients suffering from vitiligo, a chronic depigmentation disorder. Soybean phosphatidylcholine (SoyPC) liposomes were employed as model membranes to investigate if pterin (Ptr), the parent compound of oxidized pterins, is able to photoinduced lipid peroxidation. Size exclusion chromatography and dialysis experiments showed that Ptr is not encapsulated inside the liposomes and the lipid membrane is permeable to this compound. The formation of conjugated dienes and trienes, upon UVA irradiation, was followed by absorption at 234 and 270 nm, respectively. The photoproducts were characterized by mass spectrometry and oxygenation of SoyPC was demonstrated. In addition, analysis of MS/MS spectra suggested the formation hydroperoxides. Finally, the biological implications of the findings are discussed.
publishDate 2016
dc.date.none.fl_str_mv 2016
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/86070
url http://sedici.unlp.edu.ar/handle/10915/86070
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/0005-2736
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.bbamem.2015.11.002
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
139-145
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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score 13.13397

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