Photosensitization of bovine serum albumin by pterin: a mechanistic study

Autores
Thomas, Andrés Héctor; Lorente, Carolina; Roitman, Karina; Morales, Maia M.; Dantola, Maria Laura
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Pterins, heterocyclic compounds widespread in biological systems, are able to photoinduce oxidation of DNA and its components. In the present study, we have investigated the photosensitizing properties of pterin (Ptr), the parent compound of oxidized pterins, using bovine serum albumin (BSA) as target. Aqueous solutions of BSA were exposed to UV-A irradiation (350nm) in the presence of Ptr, under various experimental conditions. The photosensitized processes were followed by UV/vis spectrophotometry, an enzymatic method for H2O2 determination and electrophoresis (SDS-PAGE). We present data that demonstrate unequivocally that BSA is damaged by Ptr. Although association between Ptr and the protein was evidenced by steady-state and time-resolved fluorescence measurements, the photosensitized damage takes place via a purely dynamic mechanism, which involves an electron transfer from BSA to the triplet excited state of Ptr, formed after UV-A excitation.
Fil: Thomas, Andrés Héctor. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina. Universidad Nacional de La Plata; Argentina
Fil: Lorente, Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina. Universidad Nacional de La Plata; Argentina
Fil: Roitman, Karina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina. Universidad Nacional de La Plata; Argentina
Fil: Morales, Maia M.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina. Universidad Nacional de La Plata; Argentina
Fil: Dantola, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina. Universidad Nacional de La Plata; Argentina
Materia
Pterin
Bovine Serum Albumin
Photosensitization
Uv-A Radiation
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/5141
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/5141
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Photosensitization of bovine serum albumin by pterin: a mechanistic studyThomas, Andrés HéctorLorente, CarolinaRoitman, KarinaMorales, Maia M.Dantola, Maria LauraPterinBovine Serum AlbuminPhotosensitizationUv-A Radiationhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Pterins, heterocyclic compounds widespread in biological systems, are able to photoinduce oxidation of DNA and its components. In the present study, we have investigated the photosensitizing properties of pterin (Ptr), the parent compound of oxidized pterins, using bovine serum albumin (BSA) as target. Aqueous solutions of BSA were exposed to UV-A irradiation (350nm) in the presence of Ptr, under various experimental conditions. The photosensitized processes were followed by UV/vis spectrophotometry, an enzymatic method for H2O2 determination and electrophoresis (SDS-PAGE). We present data that demonstrate unequivocally that BSA is damaged by Ptr. Although association between Ptr and the protein was evidenced by steady-state and time-resolved fluorescence measurements, the photosensitized damage takes place via a purely dynamic mechanism, which involves an electron transfer from BSA to the triplet excited state of Ptr, formed after UV-A excitation.Fil: Thomas, Andrés Héctor. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina. Universidad Nacional de La Plata; ArgentinaFil: Lorente, Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina. Universidad Nacional de La Plata; ArgentinaFil: Roitman, Karina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina. Universidad Nacional de La Plata; ArgentinaFil: Morales, Maia M.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina. Universidad Nacional de La Plata; ArgentinaFil: Dantola, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina. Universidad Nacional de La Plata; ArgentinaElsevier2013-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/5141Thomas, Andrés Héctor; Lorente, Carolina; Roitman, Karina; Morales, Maia M.; Dantola, Maria Laura; Photosensitization of bovine serum albumin by pterin: a mechanistic study; Elsevier; Journal of Photochemistry and Photobiology B: Biology; 120; 3-2013; 52-581011-1344enginfo:eu-repo/semantics/altIdentifier/pmid/23419535info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S1011134413000146info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jphotobiol.2013.01.008info:eu-repo/semantics/altIdentifier/url/10.1016/j.jphotobiol.2013.01.008info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:11:33Zoai:ri.conicet.gov.ar:11336/5141instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:11:34.051CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Photosensitization of bovine serum albumin by pterin: a mechanistic study
title Photosensitization of bovine serum albumin by pterin: a mechanistic study
spellingShingle Photosensitization of bovine serum albumin by pterin: a mechanistic study
Thomas, Andrés Héctor
Pterin
Bovine Serum Albumin
Photosensitization
Uv-A Radiation
title_short Photosensitization of bovine serum albumin by pterin: a mechanistic study
title_full Photosensitization of bovine serum albumin by pterin: a mechanistic study
title_fullStr Photosensitization of bovine serum albumin by pterin: a mechanistic study
title_full_unstemmed Photosensitization of bovine serum albumin by pterin: a mechanistic study
title_sort Photosensitization of bovine serum albumin by pterin: a mechanistic study
dc.creator.none.fl_str_mv Thomas, Andrés Héctor
Lorente, Carolina
Roitman, Karina
Morales, Maia M.
Dantola, Maria Laura
author Thomas, Andrés Héctor
author_facet Thomas, Andrés Héctor
Lorente, Carolina
Roitman, Karina
Morales, Maia M.
Dantola, Maria Laura
author_role author
author2 Lorente, Carolina
Roitman, Karina
Morales, Maia M.
Dantola, Maria Laura
author2_role author
author
author
author
dc.subject.none.fl_str_mv Pterin
Bovine Serum Albumin
Photosensitization
Uv-A Radiation
topic Pterin
Bovine Serum Albumin
Photosensitization
Uv-A Radiation
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Pterins, heterocyclic compounds widespread in biological systems, are able to photoinduce oxidation of DNA and its components. In the present study, we have investigated the photosensitizing properties of pterin (Ptr), the parent compound of oxidized pterins, using bovine serum albumin (BSA) as target. Aqueous solutions of BSA were exposed to UV-A irradiation (350nm) in the presence of Ptr, under various experimental conditions. The photosensitized processes were followed by UV/vis spectrophotometry, an enzymatic method for H2O2 determination and electrophoresis (SDS-PAGE). We present data that demonstrate unequivocally that BSA is damaged by Ptr. Although association between Ptr and the protein was evidenced by steady-state and time-resolved fluorescence measurements, the photosensitized damage takes place via a purely dynamic mechanism, which involves an electron transfer from BSA to the triplet excited state of Ptr, formed after UV-A excitation.
Fil: Thomas, Andrés Héctor. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina. Universidad Nacional de La Plata; Argentina
Fil: Lorente, Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina. Universidad Nacional de La Plata; Argentina
Fil: Roitman, Karina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina. Universidad Nacional de La Plata; Argentina
Fil: Morales, Maia M.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina. Universidad Nacional de La Plata; Argentina
Fil: Dantola, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina. Universidad Nacional de La Plata; Argentina
description Pterins, heterocyclic compounds widespread in biological systems, are able to photoinduce oxidation of DNA and its components. In the present study, we have investigated the photosensitizing properties of pterin (Ptr), the parent compound of oxidized pterins, using bovine serum albumin (BSA) as target. Aqueous solutions of BSA were exposed to UV-A irradiation (350nm) in the presence of Ptr, under various experimental conditions. The photosensitized processes were followed by UV/vis spectrophotometry, an enzymatic method for H2O2 determination and electrophoresis (SDS-PAGE). We present data that demonstrate unequivocally that BSA is damaged by Ptr. Although association between Ptr and the protein was evidenced by steady-state and time-resolved fluorescence measurements, the photosensitized damage takes place via a purely dynamic mechanism, which involves an electron transfer from BSA to the triplet excited state of Ptr, formed after UV-A excitation.
publishDate 2013
dc.date.none.fl_str_mv 2013-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/5141
Thomas, Andrés Héctor; Lorente, Carolina; Roitman, Karina; Morales, Maia M.; Dantola, Maria Laura; Photosensitization of bovine serum albumin by pterin: a mechanistic study; Elsevier; Journal of Photochemistry and Photobiology B: Biology; 120; 3-2013; 52-58
1011-1344
url http://hdl.handle.net/11336/5141
identifier_str_mv Thomas, Andrés Héctor; Lorente, Carolina; Roitman, Karina; Morales, Maia M.; Dantola, Maria Laura; Photosensitization of bovine serum albumin by pterin: a mechanistic study; Elsevier; Journal of Photochemistry and Photobiology B: Biology; 120; 3-2013; 52-58
1011-1344
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/pmid/23419535
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S1011134413000146
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jphotobiol.2013.01.008
info:eu-repo/semantics/altIdentifier/url/10.1016/j.jphotobiol.2013.01.008
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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