Rapid preparation of affinity-purified lipopolysaccharide samples for electrophoretic analysis

Autores
Valverde, Claudio; Hozbor, Daniela Flavia; Lagares, Antonio
Año de publicación
1997
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Lipopolysaccharides (LPS) are the major components of the outer membrane of gram-negative bacteria. These surface molecules are relevant both for the outer membrane stability and for the interaction of the bacteria with other organisms and with the environment (18). Extensive literature is available concerning LPS physiology in different symbiotic (parasitic) and pathogenic host-bacterial interaction systems (5,20). Physiological, biochemical and chemical approaches to study LPS functions commonly require LPS extraction and purification. In the literature, LPS have been prepared by a number of different cell disruption procedures. Protocols for LPS isolation include organic solvent extractions followed by exhaustive dialysis or evaporation steps, and their modifications (11,22).
Facultad de Ciencias Exactas
Materia
Ciencias Exactas
Química
Gel electrophoresis
Molecular biology
Chemistry
Gram-negative bacteria
Vibrio
Polymyxin B
Salmonella
Ethylamines
Chromatography
Escherichia coli
Pharmacology
Electrophoresis, Polyacrylamide Gel
Indicators and Reagents
Rhizobium/chemistry
Indicators and Reagents
Lipopolysaccharides
Edetic Acid
triethylamine
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/122765
Acceder
id SEDICI_a1cac7addb14eb80c10736ec7efb48b2
oai_identifier_str oai:sedici.unlp.edu.ar:10915/122765
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Rapid preparation of affinity-purified lipopolysaccharide samples for electrophoretic analysisValverde, ClaudioHozbor, Daniela FlaviaLagares, AntonioCiencias ExactasQuímicaGel electrophoresisMolecular biologyChemistryGram-negative bacteriaVibrioPolymyxin BSalmonellaEthylaminesChromatographyEscherichia coliPharmacologyElectrophoresis, Polyacrylamide GelIndicators and ReagentsRhizobium/chemistryIndicators and ReagentsLipopolysaccharidesEdetic AcidtriethylamineLipopolysaccharides (LPS) are the major components of the outer membrane of gram-negative bacteria. These surface molecules are relevant both for the outer membrane stability and for the interaction of the bacteria with other organisms and with the environment (18). Extensive literature is available concerning LPS physiology in different symbiotic (parasitic) and pathogenic host-bacterial interaction systems (5,20). Physiological, biochemical and chemical approaches to study LPS functions commonly require LPS extraction and purification. In the literature, LPS have been prepared by a number of different cell disruption procedures. Protocols for LPS isolation include organic solvent extractions followed by exhaustive dialysis or evaporation steps, and their modifications (11,22).Facultad de Ciencias Exactas1997info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf230-236http://sedici.unlp.edu.ar/handle/10915/122765enginfo:eu-repo/semantics/altIdentifier/issn/0736-6205info:eu-repo/semantics/altIdentifier/issn/1940-9818info:eu-repo/semantics/altIdentifier/pmid/9043688info:eu-repo/semantics/altIdentifier/doi/10.2144/97222bm07info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:29:14Zoai:sedici.unlp.edu.ar:10915/122765Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:29:15.005SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Rapid preparation of affinity-purified lipopolysaccharide samples for electrophoretic analysis
title Rapid preparation of affinity-purified lipopolysaccharide samples for electrophoretic analysis
spellingShingle Rapid preparation of affinity-purified lipopolysaccharide samples for electrophoretic analysis
Valverde, Claudio
Ciencias Exactas
Química
Gel electrophoresis
Molecular biology
Chemistry
Gram-negative bacteria
Vibrio
Polymyxin B
Salmonella
Ethylamines
Chromatography
Escherichia coli
Pharmacology
Electrophoresis, Polyacrylamide Gel
Indicators and Reagents
Rhizobium/chemistry
Indicators and Reagents
Lipopolysaccharides
Edetic Acid
triethylamine
title_short Rapid preparation of affinity-purified lipopolysaccharide samples for electrophoretic analysis
title_full Rapid preparation of affinity-purified lipopolysaccharide samples for electrophoretic analysis
title_fullStr Rapid preparation of affinity-purified lipopolysaccharide samples for electrophoretic analysis
title_full_unstemmed Rapid preparation of affinity-purified lipopolysaccharide samples for electrophoretic analysis
title_sort Rapid preparation of affinity-purified lipopolysaccharide samples for electrophoretic analysis
dc.creator.none.fl_str_mv Valverde, Claudio
Hozbor, Daniela Flavia
Lagares, Antonio
author Valverde, Claudio
author_facet Valverde, Claudio
Hozbor, Daniela Flavia
Lagares, Antonio
author_role author
author2 Hozbor, Daniela Flavia
Lagares, Antonio
author2_role author
author
dc.subject.none.fl_str_mv Ciencias Exactas
Química
Gel electrophoresis
Molecular biology
Chemistry
Gram-negative bacteria
Vibrio
Polymyxin B
Salmonella
Ethylamines
Chromatography
Escherichia coli
Pharmacology
Electrophoresis, Polyacrylamide Gel
Indicators and Reagents
Rhizobium/chemistry
Indicators and Reagents
Lipopolysaccharides
Edetic Acid
triethylamine
topic Ciencias Exactas
Química
Gel electrophoresis
Molecular biology
Chemistry
Gram-negative bacteria
Vibrio
Polymyxin B
Salmonella
Ethylamines
Chromatography
Escherichia coli
Pharmacology
Electrophoresis, Polyacrylamide Gel
Indicators and Reagents
Rhizobium/chemistry
Indicators and Reagents
Lipopolysaccharides
Edetic Acid
triethylamine
dc.description.none.fl_txt_mv Lipopolysaccharides (LPS) are the major components of the outer membrane of gram-negative bacteria. These surface molecules are relevant both for the outer membrane stability and for the interaction of the bacteria with other organisms and with the environment (18). Extensive literature is available concerning LPS physiology in different symbiotic (parasitic) and pathogenic host-bacterial interaction systems (5,20). Physiological, biochemical and chemical approaches to study LPS functions commonly require LPS extraction and purification. In the literature, LPS have been prepared by a number of different cell disruption procedures. Protocols for LPS isolation include organic solvent extractions followed by exhaustive dialysis or evaporation steps, and their modifications (11,22).
Facultad de Ciencias Exactas
description Lipopolysaccharides (LPS) are the major components of the outer membrane of gram-negative bacteria. These surface molecules are relevant both for the outer membrane stability and for the interaction of the bacteria with other organisms and with the environment (18). Extensive literature is available concerning LPS physiology in different symbiotic (parasitic) and pathogenic host-bacterial interaction systems (5,20). Physiological, biochemical and chemical approaches to study LPS functions commonly require LPS extraction and purification. In the literature, LPS have been prepared by a number of different cell disruption procedures. Protocols for LPS isolation include organic solvent extractions followed by exhaustive dialysis or evaporation steps, and their modifications (11,22).
publishDate 1997
dc.date.none.fl_str_mv 1997
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/122765
url http://sedici.unlp.edu.ar/handle/10915/122765
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/0736-6205
info:eu-repo/semantics/altIdentifier/issn/1940-9818
info:eu-repo/semantics/altIdentifier/pmid/9043688
info:eu-repo/semantics/altIdentifier/doi/10.2144/97222bm07
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
230-236
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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score 13.070432

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