Improving Peak Capacity in Fast Online Comprehensive Two-Dimensional Liquid Chromatography with Post-First-Dimension Flow Splitting

Autores
Filgueira, Marcelo R.; Huang, Yuan; Witt, Klaus; Castells, Cecilia Beatriz Marta; Carr, Peter W.
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The use of flow splitters between the two dimensions in online comprehensive two-dimensional (2D) liquid chromatography (LC LC) has not received very much attention, in comparison with their use in 2D gas chromatography (GC GC), where they are quite common. In principle, splitting the flow after the first dimension column and performing online LC LC on this constant fraction of the first dimension effluent should allow the two dimensions to be optimized almost independently. When there is no flow splitting, any change in the first-dimension flow rate has an immediate impact on the second dimension. With a flow splitter, one could, for example, double the flow rate into the first dimension column and perform a 1:1 flow split without changing the sample loop size or the sampler’s collection time. Of course, the sensitivity would be diminished, but this can be partially compensated through the use of a larger injection; this will likely only amount to a small price to pay for this increased resolving power and system flexibility. Among other benefits, we found a 2-fold increase in the corrected 2D peak capacity and the number of observed peaks for a 15-min analysis time, using a post-first-dimension flow splitter. At a fixed analysis time, this improvement results primarily from an increase in the gradient time, resulting from the reduced system re-equilibration time, and, to a smaller extent, it is due to the increased peak capacity achieved by full optimization of the first dimension.
Facultad de Ciencias Exactas
Materia
Química
Optimization
Chromatography
Power
Computer simulations
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/123641

id SEDICI_9ecbcbf963f6374f2578ecbff2fa75e6
oai_identifier_str oai:sedici.unlp.edu.ar:10915/123641
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Improving Peak Capacity in Fast Online Comprehensive Two-Dimensional Liquid Chromatography with Post-First-Dimension Flow SplittingFilgueira, Marcelo R.Huang, YuanWitt, KlausCastells, Cecilia Beatriz MartaCarr, Peter W.QuímicaOptimizationChromatographyPowerComputer simulationsThe use of flow splitters between the two dimensions in online comprehensive two-dimensional (2D) liquid chromatography (LC LC) has not received very much attention, in comparison with their use in 2D gas chromatography (GC GC), where they are quite common. In principle, splitting the flow after the first dimension column and performing online LC LC on this constant fraction of the first dimension effluent should allow the two dimensions to be optimized almost independently. When there is no flow splitting, any change in the first-dimension flow rate has an immediate impact on the second dimension. With a flow splitter, one could, for example, double the flow rate into the first dimension column and perform a 1:1 flow split without changing the sample loop size or the sampler’s collection time. Of course, the sensitivity would be diminished, but this can be partially compensated through the use of a larger injection; this will likely only amount to a small price to pay for this increased resolving power and system flexibility. Among other benefits, we found a 2-fold increase in the corrected 2D peak capacity and the number of observed peaks for a 15-min analysis time, using a post-first-dimension flow splitter. At a fixed analysis time, this improvement results primarily from an increase in the gradient time, resulting from the reduced system re-equilibration time, and, to a smaller extent, it is due to the increased peak capacity achieved by full optimization of the first dimension.Facultad de Ciencias Exactas2011info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf9531-9539http://sedici.unlp.edu.ar/handle/10915/123641enginfo:eu-repo/semantics/altIdentifier/issn/15206882info:eu-repo/semantics/altIdentifier/issn/00032700info:eu-repo/semantics/altIdentifier/pmid/22017622info:eu-repo/semantics/altIdentifier/doi/10.1021/ac202317minfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:29:29Zoai:sedici.unlp.edu.ar:10915/123641Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:29:29.813SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Improving Peak Capacity in Fast Online Comprehensive Two-Dimensional Liquid Chromatography with Post-First-Dimension Flow Splitting
title Improving Peak Capacity in Fast Online Comprehensive Two-Dimensional Liquid Chromatography with Post-First-Dimension Flow Splitting
spellingShingle Improving Peak Capacity in Fast Online Comprehensive Two-Dimensional Liquid Chromatography with Post-First-Dimension Flow Splitting
Filgueira, Marcelo R.
Química
Optimization
Chromatography
Power
Computer simulations
title_short Improving Peak Capacity in Fast Online Comprehensive Two-Dimensional Liquid Chromatography with Post-First-Dimension Flow Splitting
title_full Improving Peak Capacity in Fast Online Comprehensive Two-Dimensional Liquid Chromatography with Post-First-Dimension Flow Splitting
title_fullStr Improving Peak Capacity in Fast Online Comprehensive Two-Dimensional Liquid Chromatography with Post-First-Dimension Flow Splitting
title_full_unstemmed Improving Peak Capacity in Fast Online Comprehensive Two-Dimensional Liquid Chromatography with Post-First-Dimension Flow Splitting
title_sort Improving Peak Capacity in Fast Online Comprehensive Two-Dimensional Liquid Chromatography with Post-First-Dimension Flow Splitting
dc.creator.none.fl_str_mv Filgueira, Marcelo R.
Huang, Yuan
Witt, Klaus
Castells, Cecilia Beatriz Marta
Carr, Peter W.
author Filgueira, Marcelo R.
author_facet Filgueira, Marcelo R.
Huang, Yuan
Witt, Klaus
Castells, Cecilia Beatriz Marta
Carr, Peter W.
author_role author
author2 Huang, Yuan
Witt, Klaus
Castells, Cecilia Beatriz Marta
Carr, Peter W.
author2_role author
author
author
author
dc.subject.none.fl_str_mv Química
Optimization
Chromatography
Power
Computer simulations
topic Química
Optimization
Chromatography
Power
Computer simulations
dc.description.none.fl_txt_mv The use of flow splitters between the two dimensions in online comprehensive two-dimensional (2D) liquid chromatography (LC LC) has not received very much attention, in comparison with their use in 2D gas chromatography (GC GC), where they are quite common. In principle, splitting the flow after the first dimension column and performing online LC LC on this constant fraction of the first dimension effluent should allow the two dimensions to be optimized almost independently. When there is no flow splitting, any change in the first-dimension flow rate has an immediate impact on the second dimension. With a flow splitter, one could, for example, double the flow rate into the first dimension column and perform a 1:1 flow split without changing the sample loop size or the sampler’s collection time. Of course, the sensitivity would be diminished, but this can be partially compensated through the use of a larger injection; this will likely only amount to a small price to pay for this increased resolving power and system flexibility. Among other benefits, we found a 2-fold increase in the corrected 2D peak capacity and the number of observed peaks for a 15-min analysis time, using a post-first-dimension flow splitter. At a fixed analysis time, this improvement results primarily from an increase in the gradient time, resulting from the reduced system re-equilibration time, and, to a smaller extent, it is due to the increased peak capacity achieved by full optimization of the first dimension.
Facultad de Ciencias Exactas
description The use of flow splitters between the two dimensions in online comprehensive two-dimensional (2D) liquid chromatography (LC LC) has not received very much attention, in comparison with their use in 2D gas chromatography (GC GC), where they are quite common. In principle, splitting the flow after the first dimension column and performing online LC LC on this constant fraction of the first dimension effluent should allow the two dimensions to be optimized almost independently. When there is no flow splitting, any change in the first-dimension flow rate has an immediate impact on the second dimension. With a flow splitter, one could, for example, double the flow rate into the first dimension column and perform a 1:1 flow split without changing the sample loop size or the sampler’s collection time. Of course, the sensitivity would be diminished, but this can be partially compensated through the use of a larger injection; this will likely only amount to a small price to pay for this increased resolving power and system flexibility. Among other benefits, we found a 2-fold increase in the corrected 2D peak capacity and the number of observed peaks for a 15-min analysis time, using a post-first-dimension flow splitter. At a fixed analysis time, this improvement results primarily from an increase in the gradient time, resulting from the reduced system re-equilibration time, and, to a smaller extent, it is due to the increased peak capacity achieved by full optimization of the first dimension.
publishDate 2011
dc.date.none.fl_str_mv 2011
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/123641
url http://sedici.unlp.edu.ar/handle/10915/123641
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/15206882
info:eu-repo/semantics/altIdentifier/issn/00032700
info:eu-repo/semantics/altIdentifier/pmid/22017622
info:eu-repo/semantics/altIdentifier/doi/10.1021/ac202317m
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
9531-9539
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
_version_ 1844616174183120896
score 13.070432