Total Antioxidant Capacity with Peak Specificity via Reaction Flow Chromatography and the Ferric Reducing Antioxidant Power Assay

Autores
Jones, Andy; Acquaviva, Agustín; Suktham, Thirada; Dennis, Gary R.; Shalliker, R. Andrew; Soliven, Arianne
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
An established ferric reducing antioxidant power (FRAP) assay was optimised by preparation of the derivatisation reagent in 300 mM formate instead of 300 mM acetate conditions, resulting in increased sensitivity signal to noise responses by up to five to ten times. The quantitative protocol for selective detection of antioxidants via a HPLC post column derivatisation (PCD) technique using the 300 mM formate FRAP reagent conditions was then transformed into a high-speed qualitative screening protocol by utilizing an emerging technology ‘reaction flow (RF) chromatography’. Reaction flow chromatography’s ability to screen for total antioxidant capacity with additional peak specificity/profile information of active peaks could be achieved in under 2 min.
Laboratorio de Investigación y Desarrollo de Métodos Analíticos (LIDMA)
Materia
Ciencias Exactas
Química
Total antioxidant capacity
Post column derivatisation
Reaction flow chromatography
Selective detection
Ferric reducing antioxidant power
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/137521
Acceder
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oai_identifier_str oai:sedici.unlp.edu.ar:10915/137521
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Total Antioxidant Capacity with Peak Specificity via Reaction Flow Chromatography and the Ferric Reducing Antioxidant Power AssayJones, AndyAcquaviva, AgustínSuktham, ThiradaDennis, Gary R.Shalliker, R. AndrewSoliven, ArianneCiencias ExactasQuímicaTotal antioxidant capacityPost column derivatisationReaction flow chromatographySelective detectionFerric reducing antioxidant powerAn established ferric reducing antioxidant power (FRAP) assay was optimised by preparation of the derivatisation reagent in 300 mM formate instead of 300 mM acetate conditions, resulting in increased sensitivity signal to noise responses by up to five to ten times. The quantitative protocol for selective detection of antioxidants via a HPLC post column derivatisation (PCD) technique using the 300 mM formate FRAP reagent conditions was then transformed into a high-speed qualitative screening protocol by utilizing an emerging technology ‘reaction flow (RF) chromatography’. Reaction flow chromatography’s ability to screen for total antioxidant capacity with additional peak specificity/profile information of active peaks could be achieved in under 2 min.Laboratorio de Investigación y Desarrollo de Métodos Analíticos (LIDMA)2020-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf608-616http://sedici.unlp.edu.ar/handle/10915/137521enginfo:eu-repo/semantics/altIdentifier/issn/1936-9751info:eu-repo/semantics/altIdentifier/issn/1936-976Xinfo:eu-repo/semantics/altIdentifier/doi/10.1007/s12161-019-01675-5info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-10-22T17:12:57Zoai:sedici.unlp.edu.ar:10915/137521Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-10-22 17:12:57.498SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Total Antioxidant Capacity with Peak Specificity via Reaction Flow Chromatography and the Ferric Reducing Antioxidant Power Assay
title Total Antioxidant Capacity with Peak Specificity via Reaction Flow Chromatography and the Ferric Reducing Antioxidant Power Assay
spellingShingle Total Antioxidant Capacity with Peak Specificity via Reaction Flow Chromatography and the Ferric Reducing Antioxidant Power Assay
Jones, Andy
Ciencias Exactas
Química
Total antioxidant capacity
Post column derivatisation
Reaction flow chromatography
Selective detection
Ferric reducing antioxidant power
title_short Total Antioxidant Capacity with Peak Specificity via Reaction Flow Chromatography and the Ferric Reducing Antioxidant Power Assay
title_full Total Antioxidant Capacity with Peak Specificity via Reaction Flow Chromatography and the Ferric Reducing Antioxidant Power Assay
title_fullStr Total Antioxidant Capacity with Peak Specificity via Reaction Flow Chromatography and the Ferric Reducing Antioxidant Power Assay
title_full_unstemmed Total Antioxidant Capacity with Peak Specificity via Reaction Flow Chromatography and the Ferric Reducing Antioxidant Power Assay
title_sort Total Antioxidant Capacity with Peak Specificity via Reaction Flow Chromatography and the Ferric Reducing Antioxidant Power Assay
dc.creator.none.fl_str_mv Jones, Andy
Acquaviva, Agustín
Suktham, Thirada
Dennis, Gary R.
Shalliker, R. Andrew
Soliven, Arianne
author Jones, Andy
author_facet Jones, Andy
Acquaviva, Agustín
Suktham, Thirada
Dennis, Gary R.
Shalliker, R. Andrew
Soliven, Arianne
author_role author
author2 Acquaviva, Agustín
Suktham, Thirada
Dennis, Gary R.
Shalliker, R. Andrew
Soliven, Arianne
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Ciencias Exactas
Química
Total antioxidant capacity
Post column derivatisation
Reaction flow chromatography
Selective detection
Ferric reducing antioxidant power
topic Ciencias Exactas
Química
Total antioxidant capacity
Post column derivatisation
Reaction flow chromatography
Selective detection
Ferric reducing antioxidant power
dc.description.none.fl_txt_mv An established ferric reducing antioxidant power (FRAP) assay was optimised by preparation of the derivatisation reagent in 300 mM formate instead of 300 mM acetate conditions, resulting in increased sensitivity signal to noise responses by up to five to ten times. The quantitative protocol for selective detection of antioxidants via a HPLC post column derivatisation (PCD) technique using the 300 mM formate FRAP reagent conditions was then transformed into a high-speed qualitative screening protocol by utilizing an emerging technology ‘reaction flow (RF) chromatography’. Reaction flow chromatography’s ability to screen for total antioxidant capacity with additional peak specificity/profile information of active peaks could be achieved in under 2 min.
Laboratorio de Investigación y Desarrollo de Métodos Analíticos (LIDMA)
description An established ferric reducing antioxidant power (FRAP) assay was optimised by preparation of the derivatisation reagent in 300 mM formate instead of 300 mM acetate conditions, resulting in increased sensitivity signal to noise responses by up to five to ten times. The quantitative protocol for selective detection of antioxidants via a HPLC post column derivatisation (PCD) technique using the 300 mM formate FRAP reagent conditions was then transformed into a high-speed qualitative screening protocol by utilizing an emerging technology ‘reaction flow (RF) chromatography’. Reaction flow chromatography’s ability to screen for total antioxidant capacity with additional peak specificity/profile information of active peaks could be achieved in under 2 min.
publishDate 2020
dc.date.none.fl_str_mv 2020-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/137521
url http://sedici.unlp.edu.ar/handle/10915/137521
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/1936-9751
info:eu-repo/semantics/altIdentifier/issn/1936-976X
info:eu-repo/semantics/altIdentifier/doi/10.1007/s12161-019-01675-5
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
dc.format.none.fl_str_mv application/pdf
608-616
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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score 12.982451

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