Total Antioxidant Capacity with Peak Specificity via Reaction Flow Chromatography and the Ferric Reducing Antioxidant Power Assay
- Autores
- Jones, Andy; Acquaviva, Agustín; Suktham, Thirada; Dennis, Gary R.; Shalliker, R. Andrew; Soliven, Arianne
- Año de publicación
- 2020
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- An established ferric reducing antioxidant power (FRAP) assay was optimised by preparation of the derivatisation reagent in 300 mM formate instead of 300 mM acetate conditions, resulting in increased sensitivity signal to noise responses by up to five to ten times. The quantitative protocol for selective detection of antioxidants via a HPLC post column derivatisation (PCD) technique using the 300 mM formate FRAP reagent conditions was then transformed into a high-speed qualitative screening protocol by utilizing an emerging technology ‘reaction flow (RF) chromatography’. Reaction flow chromatography’s ability to screen for total antioxidant capacity with additional peak specificity/profile information of active peaks could be achieved in under 2 min.
Laboratorio de Investigación y Desarrollo de Métodos Analíticos (LIDMA) - Materia
-
Ciencias Exactas
Química
Total antioxidant capacity
Post column derivatisation
Reaction flow chromatography
Selective detection
Ferric reducing antioxidant power - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by/4.0/
- Repositorio
- Institución
- Universidad Nacional de La Plata
- OAI Identificador
- oai:sedici.unlp.edu.ar:10915/137521
Ver los metadatos del registro completo
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Total Antioxidant Capacity with Peak Specificity via Reaction Flow Chromatography and the Ferric Reducing Antioxidant Power AssayJones, AndyAcquaviva, AgustínSuktham, ThiradaDennis, Gary R.Shalliker, R. AndrewSoliven, ArianneCiencias ExactasQuímicaTotal antioxidant capacityPost column derivatisationReaction flow chromatographySelective detectionFerric reducing antioxidant powerAn established ferric reducing antioxidant power (FRAP) assay was optimised by preparation of the derivatisation reagent in 300 mM formate instead of 300 mM acetate conditions, resulting in increased sensitivity signal to noise responses by up to five to ten times. The quantitative protocol for selective detection of antioxidants via a HPLC post column derivatisation (PCD) technique using the 300 mM formate FRAP reagent conditions was then transformed into a high-speed qualitative screening protocol by utilizing an emerging technology ‘reaction flow (RF) chromatography’. Reaction flow chromatography’s ability to screen for total antioxidant capacity with additional peak specificity/profile information of active peaks could be achieved in under 2 min.Laboratorio de Investigación y Desarrollo de Métodos Analíticos (LIDMA)2020-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf608-616http://sedici.unlp.edu.ar/handle/10915/137521enginfo:eu-repo/semantics/altIdentifier/issn/1936-9751info:eu-repo/semantics/altIdentifier/issn/1936-976Xinfo:eu-repo/semantics/altIdentifier/doi/10.1007/s12161-019-01675-5info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:32:02Zoai:sedici.unlp.edu.ar:10915/137521Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:32:03.184SEDICI (UNLP) - Universidad Nacional de La Platafalse |
dc.title.none.fl_str_mv |
Total Antioxidant Capacity with Peak Specificity via Reaction Flow Chromatography and the Ferric Reducing Antioxidant Power Assay |
title |
Total Antioxidant Capacity with Peak Specificity via Reaction Flow Chromatography and the Ferric Reducing Antioxidant Power Assay |
spellingShingle |
Total Antioxidant Capacity with Peak Specificity via Reaction Flow Chromatography and the Ferric Reducing Antioxidant Power Assay Jones, Andy Ciencias Exactas Química Total antioxidant capacity Post column derivatisation Reaction flow chromatography Selective detection Ferric reducing antioxidant power |
title_short |
Total Antioxidant Capacity with Peak Specificity via Reaction Flow Chromatography and the Ferric Reducing Antioxidant Power Assay |
title_full |
Total Antioxidant Capacity with Peak Specificity via Reaction Flow Chromatography and the Ferric Reducing Antioxidant Power Assay |
title_fullStr |
Total Antioxidant Capacity with Peak Specificity via Reaction Flow Chromatography and the Ferric Reducing Antioxidant Power Assay |
title_full_unstemmed |
Total Antioxidant Capacity with Peak Specificity via Reaction Flow Chromatography and the Ferric Reducing Antioxidant Power Assay |
title_sort |
Total Antioxidant Capacity with Peak Specificity via Reaction Flow Chromatography and the Ferric Reducing Antioxidant Power Assay |
dc.creator.none.fl_str_mv |
Jones, Andy Acquaviva, Agustín Suktham, Thirada Dennis, Gary R. Shalliker, R. Andrew Soliven, Arianne |
author |
Jones, Andy |
author_facet |
Jones, Andy Acquaviva, Agustín Suktham, Thirada Dennis, Gary R. Shalliker, R. Andrew Soliven, Arianne |
author_role |
author |
author2 |
Acquaviva, Agustín Suktham, Thirada Dennis, Gary R. Shalliker, R. Andrew Soliven, Arianne |
author2_role |
author author author author author |
dc.subject.none.fl_str_mv |
Ciencias Exactas Química Total antioxidant capacity Post column derivatisation Reaction flow chromatography Selective detection Ferric reducing antioxidant power |
topic |
Ciencias Exactas Química Total antioxidant capacity Post column derivatisation Reaction flow chromatography Selective detection Ferric reducing antioxidant power |
dc.description.none.fl_txt_mv |
An established ferric reducing antioxidant power (FRAP) assay was optimised by preparation of the derivatisation reagent in 300 mM formate instead of 300 mM acetate conditions, resulting in increased sensitivity signal to noise responses by up to five to ten times. The quantitative protocol for selective detection of antioxidants via a HPLC post column derivatisation (PCD) technique using the 300 mM formate FRAP reagent conditions was then transformed into a high-speed qualitative screening protocol by utilizing an emerging technology ‘reaction flow (RF) chromatography’. Reaction flow chromatography’s ability to screen for total antioxidant capacity with additional peak specificity/profile information of active peaks could be achieved in under 2 min. Laboratorio de Investigación y Desarrollo de Métodos Analíticos (LIDMA) |
description |
An established ferric reducing antioxidant power (FRAP) assay was optimised by preparation of the derivatisation reagent in 300 mM formate instead of 300 mM acetate conditions, resulting in increased sensitivity signal to noise responses by up to five to ten times. The quantitative protocol for selective detection of antioxidants via a HPLC post column derivatisation (PCD) technique using the 300 mM formate FRAP reagent conditions was then transformed into a high-speed qualitative screening protocol by utilizing an emerging technology ‘reaction flow (RF) chromatography’. Reaction flow chromatography’s ability to screen for total antioxidant capacity with additional peak specificity/profile information of active peaks could be achieved in under 2 min. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-03 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Articulo http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://sedici.unlp.edu.ar/handle/10915/137521 |
url |
http://sedici.unlp.edu.ar/handle/10915/137521 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/issn/1936-9751 info:eu-repo/semantics/altIdentifier/issn/1936-976X info:eu-repo/semantics/altIdentifier/doi/10.1007/s12161-019-01675-5 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/4.0/ Creative Commons Attribution 4.0 International (CC BY 4.0) |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by/4.0/ Creative Commons Attribution 4.0 International (CC BY 4.0) |
dc.format.none.fl_str_mv |
application/pdf 608-616 |
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reponame:SEDICI (UNLP) instname:Universidad Nacional de La Plata instacron:UNLP |
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SEDICI (UNLP) |
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Universidad Nacional de La Plata |
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UNLP |
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SEDICI (UNLP) - Universidad Nacional de La Plata |
repository.mail.fl_str_mv |
alira@sedici.unlp.edu.ar |
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13.070432 |