Improving peak capacity in fast online comprehensive two-dimensional liquid chromatography with post-first-dimension flow splitting
- Autores
- Filgueira, Marcelo R.; Huang, Yuan; Witt, Klaus; Castells, Cecilia Beatriz Marta; Carr, Peter W.
- Año de publicación
- 2011
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The use of flow splitters between the two dimensions in online comprehensive two-dimensional (2D) liquid chromatography (LC × LC) has not received very much attention, in comparison with their use in 2D gas chromatography (GC × GC), where they are quite common. In principle, splitting the flow after the first dimension column and performing online LC × LC on this constant fraction of the first dimension effluent should allow the two dimensions to be optimized almost independently. When there is no flow splitting, any change in the first-dimension flow rate has an immediate impact on the second dimension. With a flow splitter, one could, for example, double the flow rate into the first dimension column and perform a 1:1 flow split without changing the sample loop size or the sampler's collection time. Of course, the sensitivity would be diminished, but this can be partially compensated through the use of a larger injection; this will likely only amount to a small price to pay for this increased resolving power and system flexibility. Among other benefits, we found a 2-fold increase in the corrected 2D peak capacity and the number of observed peaks for a 15-min analysis time, using a post-first-dimension flow splitter. At a fixed analysis time, this improvement results primarily from an increase in the gradient time, resulting from the reduced system re-equilibration time, and, to a smaller extent, it is due to the increased peak capacity achieved by full optimization of the first dimension.
Fil: Filgueira, Marcelo R.. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; Argentina. University of Minnesota; Estados Unidos
Fil: Huang, Yuan. University of Minnesota; Estados Unidos
Fil: Witt, Klaus. Agilent Technologies Deutschland; Alemania
Fil: Castells, Cecilia Beatriz Marta. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigaciones en Tecnología de Pinturas. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigaciones en Tecnología de Pinturas; Argentina
Fil: Carr, Peter W.. University of Minnesota; Estados Unidos - Materia
-
Two dimensional LC
Peak capacity
Fast Gradient
Flow Splitting - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
.jpg)
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/95499
Ver los metadatos del registro completo
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Improving peak capacity in fast online comprehensive two-dimensional liquid chromatography with post-first-dimension flow splittingFilgueira, Marcelo R.Huang, YuanWitt, KlausCastells, Cecilia Beatriz MartaCarr, Peter W.Two dimensional LCPeak capacityFast GradientFlow Splittinghttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1The use of flow splitters between the two dimensions in online comprehensive two-dimensional (2D) liquid chromatography (LC × LC) has not received very much attention, in comparison with their use in 2D gas chromatography (GC × GC), where they are quite common. In principle, splitting the flow after the first dimension column and performing online LC × LC on this constant fraction of the first dimension effluent should allow the two dimensions to be optimized almost independently. When there is no flow splitting, any change in the first-dimension flow rate has an immediate impact on the second dimension. With a flow splitter, one could, for example, double the flow rate into the first dimension column and perform a 1:1 flow split without changing the sample loop size or the sampler's collection time. Of course, the sensitivity would be diminished, but this can be partially compensated through the use of a larger injection; this will likely only amount to a small price to pay for this increased resolving power and system flexibility. Among other benefits, we found a 2-fold increase in the corrected 2D peak capacity and the number of observed peaks for a 15-min analysis time, using a post-first-dimension flow splitter. At a fixed analysis time, this improvement results primarily from an increase in the gradient time, resulting from the reduced system re-equilibration time, and, to a smaller extent, it is due to the increased peak capacity achieved by full optimization of the first dimension.Fil: Filgueira, Marcelo R.. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; Argentina. University of Minnesota; Estados UnidosFil: Huang, Yuan. University of Minnesota; Estados UnidosFil: Witt, Klaus. Agilent Technologies Deutschland; AlemaniaFil: Castells, Cecilia Beatriz Marta. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigaciones en Tecnología de Pinturas. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigaciones en Tecnología de Pinturas; ArgentinaFil: Carr, Peter W.. University of Minnesota; Estados UnidosAmerican Chemical Society2011-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/95499Filgueira, Marcelo R.; Huang, Yuan; Witt, Klaus; Castells, Cecilia Beatriz Marta; Carr, Peter W.; Improving peak capacity in fast online comprehensive two-dimensional liquid chromatography with post-first-dimension flow splitting; American Chemical Society; Analytical Chemistry; 83; 24; 12-2011; 9531-95390003-2700CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://pubs.acs.org/doi/10.1021/ac202317minfo:eu-repo/semantics/altIdentifier/doi/10.1021/ac202317minfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:26:45Zoai:ri.conicet.gov.ar:11336/95499instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:26:45.94CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
| dc.title.none.fl_str_mv |
Improving peak capacity in fast online comprehensive two-dimensional liquid chromatography with post-first-dimension flow splitting |
| title |
Improving peak capacity in fast online comprehensive two-dimensional liquid chromatography with post-first-dimension flow splitting |
| spellingShingle |
Improving peak capacity in fast online comprehensive two-dimensional liquid chromatography with post-first-dimension flow splitting Filgueira, Marcelo R. Two dimensional LC Peak capacity Fast Gradient Flow Splitting |
| title_short |
Improving peak capacity in fast online comprehensive two-dimensional liquid chromatography with post-first-dimension flow splitting |
| title_full |
Improving peak capacity in fast online comprehensive two-dimensional liquid chromatography with post-first-dimension flow splitting |
| title_fullStr |
Improving peak capacity in fast online comprehensive two-dimensional liquid chromatography with post-first-dimension flow splitting |
| title_full_unstemmed |
Improving peak capacity in fast online comprehensive two-dimensional liquid chromatography with post-first-dimension flow splitting |
| title_sort |
Improving peak capacity in fast online comprehensive two-dimensional liquid chromatography with post-first-dimension flow splitting |
| dc.creator.none.fl_str_mv |
Filgueira, Marcelo R. Huang, Yuan Witt, Klaus Castells, Cecilia Beatriz Marta Carr, Peter W. |
| author |
Filgueira, Marcelo R. |
| author_facet |
Filgueira, Marcelo R. Huang, Yuan Witt, Klaus Castells, Cecilia Beatriz Marta Carr, Peter W. |
| author_role |
author |
| author2 |
Huang, Yuan Witt, Klaus Castells, Cecilia Beatriz Marta Carr, Peter W. |
| author2_role |
author author author author |
| dc.subject.none.fl_str_mv |
Two dimensional LC Peak capacity Fast Gradient Flow Splitting |
| topic |
Two dimensional LC Peak capacity Fast Gradient Flow Splitting |
| purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.4 https://purl.org/becyt/ford/1 |
| dc.description.none.fl_txt_mv |
The use of flow splitters between the two dimensions in online comprehensive two-dimensional (2D) liquid chromatography (LC × LC) has not received very much attention, in comparison with their use in 2D gas chromatography (GC × GC), where they are quite common. In principle, splitting the flow after the first dimension column and performing online LC × LC on this constant fraction of the first dimension effluent should allow the two dimensions to be optimized almost independently. When there is no flow splitting, any change in the first-dimension flow rate has an immediate impact on the second dimension. With a flow splitter, one could, for example, double the flow rate into the first dimension column and perform a 1:1 flow split without changing the sample loop size or the sampler's collection time. Of course, the sensitivity would be diminished, but this can be partially compensated through the use of a larger injection; this will likely only amount to a small price to pay for this increased resolving power and system flexibility. Among other benefits, we found a 2-fold increase in the corrected 2D peak capacity and the number of observed peaks for a 15-min analysis time, using a post-first-dimension flow splitter. At a fixed analysis time, this improvement results primarily from an increase in the gradient time, resulting from the reduced system re-equilibration time, and, to a smaller extent, it is due to the increased peak capacity achieved by full optimization of the first dimension. Fil: Filgueira, Marcelo R.. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; Argentina. University of Minnesota; Estados Unidos Fil: Huang, Yuan. University of Minnesota; Estados Unidos Fil: Witt, Klaus. Agilent Technologies Deutschland; Alemania Fil: Castells, Cecilia Beatriz Marta. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigaciones en Tecnología de Pinturas. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigaciones en Tecnología de Pinturas; Argentina Fil: Carr, Peter W.. University of Minnesota; Estados Unidos |
| description |
The use of flow splitters between the two dimensions in online comprehensive two-dimensional (2D) liquid chromatography (LC × LC) has not received very much attention, in comparison with their use in 2D gas chromatography (GC × GC), where they are quite common. In principle, splitting the flow after the first dimension column and performing online LC × LC on this constant fraction of the first dimension effluent should allow the two dimensions to be optimized almost independently. When there is no flow splitting, any change in the first-dimension flow rate has an immediate impact on the second dimension. With a flow splitter, one could, for example, double the flow rate into the first dimension column and perform a 1:1 flow split without changing the sample loop size or the sampler's collection time. Of course, the sensitivity would be diminished, but this can be partially compensated through the use of a larger injection; this will likely only amount to a small price to pay for this increased resolving power and system flexibility. Among other benefits, we found a 2-fold increase in the corrected 2D peak capacity and the number of observed peaks for a 15-min analysis time, using a post-first-dimension flow splitter. At a fixed analysis time, this improvement results primarily from an increase in the gradient time, resulting from the reduced system re-equilibration time, and, to a smaller extent, it is due to the increased peak capacity achieved by full optimization of the first dimension. |
| publishDate |
2011 |
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2011-12 |
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http://hdl.handle.net/11336/95499 Filgueira, Marcelo R.; Huang, Yuan; Witt, Klaus; Castells, Cecilia Beatriz Marta; Carr, Peter W.; Improving peak capacity in fast online comprehensive two-dimensional liquid chromatography with post-first-dimension flow splitting; American Chemical Society; Analytical Chemistry; 83; 24; 12-2011; 9531-9539 0003-2700 CONICET Digital CONICET |
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http://hdl.handle.net/11336/95499 |
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Filgueira, Marcelo R.; Huang, Yuan; Witt, Klaus; Castells, Cecilia Beatriz Marta; Carr, Peter W.; Improving peak capacity in fast online comprehensive two-dimensional liquid chromatography with post-first-dimension flow splitting; American Chemical Society; Analytical Chemistry; 83; 24; 12-2011; 9531-9539 0003-2700 CONICET Digital CONICET |
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