Muc5ac mucin expression during rat skin development

Autores
Ferretti, Valeria Alejandra; Segal-Eiras, Amada; Barbeito, Claudio Gustavo; Croce, María Virginia
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Some mucin genes have been detected during human embryonic and fetal organ development; however, little is known about mucin expression in epidermal development, neither in humans nor in other species. The present research was developed to explore Muc5ac skin expression during pre-and post-natal rat development. Immunohistochemistry (IHC), Western blotting (WB) and RT-PCR were employed. By IHC, Muc5ac protein was found early in embryonic epidermis from day 13 of gestation until seven days after birth when the surface epidermis became negative and the reaction was restricted to secreting sebum cells. In coincidence with IHC findings, WB analysis showed a band at approximately 200KDa at the same periods of development. Results were also confirmed by RT-PCR. Muc5ac expression in rat embryonic epidermis suggests that Muc5ac may play a protective role in embryonic skin previous to birth which may be replaced by pile covering. To our knowledge, this is the first report that confirmed Muc5ac expression during skin development. and airways epithelia.2 It is common knowledge that normal skin exhibits a rather restricted expression of mucins, and it has been demonstrated that epithelial cells of the normal epidermis do not express sialomucins while Muc5ac expression in cancer epidermal cells is well documented.4,5 On the other hand, little is known about mucin expression in epidermal development, neither in humans nor in other species. However, some mucin genes have been detected during human embryonic and fetal organ development according to differential expression patterns in comparison with adult tissues.3,6 The present research was developed to explore Muc5ac skin expression during preand post-natal rat development.
Centro de Investigaciones Inmunológicas Básicas y Aplicadas
Facultad de Ciencias Veterinarias
Materia
Biología
Development
Muc5ac
Rat
Skin
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/85831
Acceder
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oai_identifier_str oai:sedici.unlp.edu.ar:10915/85831
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Muc5ac mucin expression during rat skin developmentFerretti, Valeria AlejandraSegal-Eiras, AmadaBarbeito, Claudio GustavoCroce, María VirginiaBiologíaDevelopmentMuc5acRatSkinSome mucin genes have been detected during human embryonic and fetal organ development; however, little is known about mucin expression in epidermal development, neither in humans nor in other species. The present research was developed to explore Muc5ac skin expression during pre-and post-natal rat development. Immunohistochemistry (IHC), Western blotting (WB) and RT-PCR were employed. By IHC, Muc5ac protein was found early in embryonic epidermis from day 13 of gestation until seven days after birth when the surface epidermis became negative and the reaction was restricted to secreting sebum cells. In coincidence with IHC findings, WB analysis showed a band at approximately 200KDa at the same periods of development. Results were also confirmed by RT-PCR. Muc5ac expression in rat embryonic epidermis suggests that Muc5ac may play a protective role in embryonic skin previous to birth which may be replaced by pile covering. To our knowledge, this is the first report that confirmed Muc5ac expression during skin development. and airways epithelia.2 It is common knowledge that normal skin exhibits a rather restricted expression of mucins, and it has been demonstrated that epithelial cells of the normal epidermis do not express sialomucins while Muc5ac expression in cancer epidermal cells is well documented.4,5 On the other hand, little is known about mucin expression in epidermal development, neither in humans nor in other species. However, some mucin genes have been detected during human embryonic and fetal organ development according to differential expression patterns in comparison with adult tissues.3,6 The present research was developed to explore Muc5ac skin expression during preand post-natal rat development.Centro de Investigaciones Inmunológicas Básicas y AplicadasFacultad de Ciencias Veterinarias2015info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf22-27http://sedici.unlp.edu.ar/handle/10915/85831enginfo:eu-repo/semantics/altIdentifier/issn/1121-760Xinfo:eu-repo/semantics/altIdentifier/doi/10.4081/ejh.2015.2462info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc/4.0/Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-10-22T16:57:46Zoai:sedici.unlp.edu.ar:10915/85831Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-10-22 16:57:47.271SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Muc5ac mucin expression during rat skin development
title Muc5ac mucin expression during rat skin development
spellingShingle Muc5ac mucin expression during rat skin development
Ferretti, Valeria Alejandra
Biología
Development
Muc5ac
Rat
Skin
title_short Muc5ac mucin expression during rat skin development
title_full Muc5ac mucin expression during rat skin development
title_fullStr Muc5ac mucin expression during rat skin development
title_full_unstemmed Muc5ac mucin expression during rat skin development
title_sort Muc5ac mucin expression during rat skin development
dc.creator.none.fl_str_mv Ferretti, Valeria Alejandra
Segal-Eiras, Amada
Barbeito, Claudio Gustavo
Croce, María Virginia
author Ferretti, Valeria Alejandra
author_facet Ferretti, Valeria Alejandra
Segal-Eiras, Amada
Barbeito, Claudio Gustavo
Croce, María Virginia
author_role author
author2 Segal-Eiras, Amada
Barbeito, Claudio Gustavo
Croce, María Virginia
author2_role author
author
author
dc.subject.none.fl_str_mv Biología
Development
Muc5ac
Rat
Skin
topic Biología
Development
Muc5ac
Rat
Skin
dc.description.none.fl_txt_mv Some mucin genes have been detected during human embryonic and fetal organ development; however, little is known about mucin expression in epidermal development, neither in humans nor in other species. The present research was developed to explore Muc5ac skin expression during pre-and post-natal rat development. Immunohistochemistry (IHC), Western blotting (WB) and RT-PCR were employed. By IHC, Muc5ac protein was found early in embryonic epidermis from day 13 of gestation until seven days after birth when the surface epidermis became negative and the reaction was restricted to secreting sebum cells. In coincidence with IHC findings, WB analysis showed a band at approximately 200KDa at the same periods of development. Results were also confirmed by RT-PCR. Muc5ac expression in rat embryonic epidermis suggests that Muc5ac may play a protective role in embryonic skin previous to birth which may be replaced by pile covering. To our knowledge, this is the first report that confirmed Muc5ac expression during skin development. and airways epithelia.2 It is common knowledge that normal skin exhibits a rather restricted expression of mucins, and it has been demonstrated that epithelial cells of the normal epidermis do not express sialomucins while Muc5ac expression in cancer epidermal cells is well documented.4,5 On the other hand, little is known about mucin expression in epidermal development, neither in humans nor in other species. However, some mucin genes have been detected during human embryonic and fetal organ development according to differential expression patterns in comparison with adult tissues.3,6 The present research was developed to explore Muc5ac skin expression during preand post-natal rat development.
Centro de Investigaciones Inmunológicas Básicas y Aplicadas
Facultad de Ciencias Veterinarias
description Some mucin genes have been detected during human embryonic and fetal organ development; however, little is known about mucin expression in epidermal development, neither in humans nor in other species. The present research was developed to explore Muc5ac skin expression during pre-and post-natal rat development. Immunohistochemistry (IHC), Western blotting (WB) and RT-PCR were employed. By IHC, Muc5ac protein was found early in embryonic epidermis from day 13 of gestation until seven days after birth when the surface epidermis became negative and the reaction was restricted to secreting sebum cells. In coincidence with IHC findings, WB analysis showed a band at approximately 200KDa at the same periods of development. Results were also confirmed by RT-PCR. Muc5ac expression in rat embryonic epidermis suggests that Muc5ac may play a protective role in embryonic skin previous to birth which may be replaced by pile covering. To our knowledge, this is the first report that confirmed Muc5ac expression during skin development. and airways epithelia.2 It is common knowledge that normal skin exhibits a rather restricted expression of mucins, and it has been demonstrated that epithelial cells of the normal epidermis do not express sialomucins while Muc5ac expression in cancer epidermal cells is well documented.4,5 On the other hand, little is known about mucin expression in epidermal development, neither in humans nor in other species. However, some mucin genes have been detected during human embryonic and fetal organ development according to differential expression patterns in comparison with adult tissues.3,6 The present research was developed to explore Muc5ac skin expression during preand post-natal rat development.
publishDate 2015
dc.date.none.fl_str_mv 2015
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/85831
url http://sedici.unlp.edu.ar/handle/10915/85831
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/1121-760X
info:eu-repo/semantics/altIdentifier/doi/10.4081/ejh.2015.2462
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc/4.0/
Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc/4.0/
Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)
dc.format.none.fl_str_mv application/pdf
22-27
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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