Immunohistochemical evidence of Muc1 expression during rat embryonic development
- Autores
- Lacunza, Ezequiel; Ferretti, Valeria Alejandra; Barbeito, Claudio Gustavo; Segal-Eiras, Amada; Croce, María Virginia
- Año de publicación
- 2010
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- During embryonic development, studies on mouse and human embryos have established that Muc1/MUC1 expression coincides with the onset of epithelial sheet and glandular formation. This study aimed therefore at evaluating the temporal and spatial expression of Muc1 at different stages of rat development. In this report, 80 animals were included: 64 rat foetuses at 13, 14, 15, 16, 17, 18, 19 and 20 days of gestation from pregnant females (WKAH/Hok), 8 embryos each stage. Standard immunohistochemistry was performed using anti-MUC1 cytoplasmic tail polyclonal antibody (CT33). The reaction was considered positive when more than 5% of the cells were stained; reaction patterns were: L = linear, membrane, C = cytoplasmic and M = mixed; nuclear staining was also recorded. Intensity was graded as negative (-), low (+), moderate (++) and strong (+++). Muc1 expression was observed with a low intensity on 13th day (13 D) in the stomach, lung and kidney; at 14 d, small intestine and pancreas were also reactive; at 16 D, liver and esophagus and at 18 D, trachea and salivary glands. During the development, intensity increased while the pattern of expression changed: at the first days of gestation, it was predominantly linear and apical while during further development an increase in cytoplasmic expression was observed. Trachea, stomach, kidney and lung epithelia were the more reactive tissues. In specimens belonging to neonates and adults, all tissues analyzed showed similar Muc1 expression. The findings of this study assess that Muc1 is highly expressed in the epithelial rat embryonic development.
Facultad de Ciencias Veterinarias
Centro de Investigaciones Inmunológicas Básicas y Aplicadas
Facultad de Ciencias Médicas - Materia
-
Ciencias Veterinarias
Ciencias Médicas
Embryonic development
Immunohistochemistry
Muc1/MUC1
Rat - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by-nc/3.0/
- Repositorio
- Institución
- Universidad Nacional de La Plata
- OAI Identificador
- oai:sedici.unlp.edu.ar:10915/82440
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Immunohistochemical evidence of Muc1 expression during rat embryonic developmentLacunza, EzequielFerretti, Valeria AlejandraBarbeito, Claudio GustavoSegal-Eiras, AmadaCroce, María VirginiaCiencias VeterinariasCiencias MédicasEmbryonic developmentImmunohistochemistryMuc1/MUC1RatDuring embryonic development, studies on mouse and human embryos have established that Muc1/MUC1 expression coincides with the onset of epithelial sheet and glandular formation. This study aimed therefore at evaluating the temporal and spatial expression of Muc1 at different stages of rat development. In this report, 80 animals were included: 64 rat foetuses at 13, 14, 15, 16, 17, 18, 19 and 20 days of gestation from pregnant females (WKAH/Hok), 8 embryos each stage. Standard immunohistochemistry was performed using anti-MUC1 cytoplasmic tail polyclonal antibody (CT33). The reaction was considered positive when more than 5% of the cells were stained; reaction patterns were: L = linear, membrane, C = cytoplasmic and M = mixed; nuclear staining was also recorded. Intensity was graded as negative (-), low (+), moderate (++) and strong (+++). Muc1 expression was observed with a low intensity on 13th day (13 D) in the stomach, lung and kidney; at 14 d, small intestine and pancreas were also reactive; at 16 D, liver and esophagus and at 18 D, trachea and salivary glands. During the development, intensity increased while the pattern of expression changed: at the first days of gestation, it was predominantly linear and apical while during further development an increase in cytoplasmic expression was observed. Trachea, stomach, kidney and lung epithelia were the more reactive tissues. In specimens belonging to neonates and adults, all tissues analyzed showed similar Muc1 expression. The findings of this study assess that Muc1 is highly expressed in the epithelial rat embryonic development.Facultad de Ciencias VeterinariasCentro de Investigaciones Inmunológicas Básicas y AplicadasFacultad de Ciencias Médicas2010info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf223-228http://sedici.unlp.edu.ar/handle/10915/82440enginfo:eu-repo/semantics/altIdentifier/issn/1121760Xinfo:eu-repo/semantics/altIdentifier/doi/10.4081/ejh.2010.e49info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc/3.0/Creative Commons Attribution-NonCommercial 3.0 Unported (CC BY-NC 3.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:15:31Zoai:sedici.unlp.edu.ar:10915/82440Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:15:31.453SEDICI (UNLP) - Universidad Nacional de La Platafalse |
dc.title.none.fl_str_mv |
Immunohistochemical evidence of Muc1 expression during rat embryonic development |
title |
Immunohistochemical evidence of Muc1 expression during rat embryonic development |
spellingShingle |
Immunohistochemical evidence of Muc1 expression during rat embryonic development Lacunza, Ezequiel Ciencias Veterinarias Ciencias Médicas Embryonic development Immunohistochemistry Muc1/MUC1 Rat |
title_short |
Immunohistochemical evidence of Muc1 expression during rat embryonic development |
title_full |
Immunohistochemical evidence of Muc1 expression during rat embryonic development |
title_fullStr |
Immunohistochemical evidence of Muc1 expression during rat embryonic development |
title_full_unstemmed |
Immunohistochemical evidence of Muc1 expression during rat embryonic development |
title_sort |
Immunohistochemical evidence of Muc1 expression during rat embryonic development |
dc.creator.none.fl_str_mv |
Lacunza, Ezequiel Ferretti, Valeria Alejandra Barbeito, Claudio Gustavo Segal-Eiras, Amada Croce, María Virginia |
author |
Lacunza, Ezequiel |
author_facet |
Lacunza, Ezequiel Ferretti, Valeria Alejandra Barbeito, Claudio Gustavo Segal-Eiras, Amada Croce, María Virginia |
author_role |
author |
author2 |
Ferretti, Valeria Alejandra Barbeito, Claudio Gustavo Segal-Eiras, Amada Croce, María Virginia |
author2_role |
author author author author |
dc.subject.none.fl_str_mv |
Ciencias Veterinarias Ciencias Médicas Embryonic development Immunohistochemistry Muc1/MUC1 Rat |
topic |
Ciencias Veterinarias Ciencias Médicas Embryonic development Immunohistochemistry Muc1/MUC1 Rat |
dc.description.none.fl_txt_mv |
During embryonic development, studies on mouse and human embryos have established that Muc1/MUC1 expression coincides with the onset of epithelial sheet and glandular formation. This study aimed therefore at evaluating the temporal and spatial expression of Muc1 at different stages of rat development. In this report, 80 animals were included: 64 rat foetuses at 13, 14, 15, 16, 17, 18, 19 and 20 days of gestation from pregnant females (WKAH/Hok), 8 embryos each stage. Standard immunohistochemistry was performed using anti-MUC1 cytoplasmic tail polyclonal antibody (CT33). The reaction was considered positive when more than 5% of the cells were stained; reaction patterns were: L = linear, membrane, C = cytoplasmic and M = mixed; nuclear staining was also recorded. Intensity was graded as negative (-), low (+), moderate (++) and strong (+++). Muc1 expression was observed with a low intensity on 13th day (13 D) in the stomach, lung and kidney; at 14 d, small intestine and pancreas were also reactive; at 16 D, liver and esophagus and at 18 D, trachea and salivary glands. During the development, intensity increased while the pattern of expression changed: at the first days of gestation, it was predominantly linear and apical while during further development an increase in cytoplasmic expression was observed. Trachea, stomach, kidney and lung epithelia were the more reactive tissues. In specimens belonging to neonates and adults, all tissues analyzed showed similar Muc1 expression. The findings of this study assess that Muc1 is highly expressed in the epithelial rat embryonic development. Facultad de Ciencias Veterinarias Centro de Investigaciones Inmunológicas Básicas y Aplicadas Facultad de Ciencias Médicas |
description |
During embryonic development, studies on mouse and human embryos have established that Muc1/MUC1 expression coincides with the onset of epithelial sheet and glandular formation. This study aimed therefore at evaluating the temporal and spatial expression of Muc1 at different stages of rat development. In this report, 80 animals were included: 64 rat foetuses at 13, 14, 15, 16, 17, 18, 19 and 20 days of gestation from pregnant females (WKAH/Hok), 8 embryos each stage. Standard immunohistochemistry was performed using anti-MUC1 cytoplasmic tail polyclonal antibody (CT33). The reaction was considered positive when more than 5% of the cells were stained; reaction patterns were: L = linear, membrane, C = cytoplasmic and M = mixed; nuclear staining was also recorded. Intensity was graded as negative (-), low (+), moderate (++) and strong (+++). Muc1 expression was observed with a low intensity on 13th day (13 D) in the stomach, lung and kidney; at 14 d, small intestine and pancreas were also reactive; at 16 D, liver and esophagus and at 18 D, trachea and salivary glands. During the development, intensity increased while the pattern of expression changed: at the first days of gestation, it was predominantly linear and apical while during further development an increase in cytoplasmic expression was observed. Trachea, stomach, kidney and lung epithelia were the more reactive tissues. In specimens belonging to neonates and adults, all tissues analyzed showed similar Muc1 expression. The findings of this study assess that Muc1 is highly expressed in the epithelial rat embryonic development. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Articulo http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://sedici.unlp.edu.ar/handle/10915/82440 |
url |
http://sedici.unlp.edu.ar/handle/10915/82440 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
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info:eu-repo/semantics/altIdentifier/issn/1121760X info:eu-repo/semantics/altIdentifier/doi/10.4081/ejh.2010.e49 |
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info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc/3.0/ Creative Commons Attribution-NonCommercial 3.0 Unported (CC BY-NC 3.0) |
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openAccess |
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