Immunohistochemical evidence of Muc1 expression during rat embryonic development

Autores
Lacunza, Ezequiel; Ferretti, Valeria Alejandra; Barbeito, Claudio Gustavo; Segal-Eiras, Amada; Croce, María Virginia
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
During embryonic development, studies on mouse and human embryos have established that Muc1/MUC1 expression coincides with the onset of epithelial sheet and glandular formation. This study aimed therefore at evaluating the temporal and spatial expression of Muc1 at different stages of rat development. In this report, 80 animals were included: 64 rat foetuses at 13, 14, 15, 16, 17, 18, 19 and 20 days of gestation from pregnant females (WKAH/Hok), 8 embryos each stage. Standard immunohistochemistry was performed using anti-MUC1 cytoplasmic tail polyclonal antibody (CT33). The reaction was considered positive when more than 5% of the cells were stained; reaction patterns were: L = linear, membrane, C = cytoplasmic and M = mixed; nuclear staining was also recorded. Intensity was graded as negative (-), low (+), moderate (++) and strong (+++). Muc1 expression was observed with a low intensity on 13th day (13 D) in the stomach, lung and kidney; at 14 d, small intestine and pancreas were also reactive; at 16 D, liver and esophagus and at 18 D, trachea and salivary glands. During the development, intensity increased while the pattern of expression changed: at the first days of gestation, it was predominantly linear and apical while during further development an increase in cytoplasmic expression was observed. Trachea, stomach, kidney and lung epithelia were the more reactive tissues. In specimens belonging to neonates and adults, all tissues analyzed showed similar Muc1 expression. The findings of this study assess that Muc1 is highly expressed in the epithelial rat embryonic development.
Facultad de Ciencias Veterinarias
Centro de Investigaciones Inmunológicas Básicas y Aplicadas
Facultad de Ciencias Médicas
Materia
Ciencias Veterinarias
Ciencias Médicas
Embryonic development
Immunohistochemistry
Muc1/MUC1
Rat
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc/3.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/82440

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network_name_str SEDICI (UNLP)
spelling Immunohistochemical evidence of Muc1 expression during rat embryonic developmentLacunza, EzequielFerretti, Valeria AlejandraBarbeito, Claudio GustavoSegal-Eiras, AmadaCroce, María VirginiaCiencias VeterinariasCiencias MédicasEmbryonic developmentImmunohistochemistryMuc1/MUC1RatDuring embryonic development, studies on mouse and human embryos have established that Muc1/MUC1 expression coincides with the onset of epithelial sheet and glandular formation. This study aimed therefore at evaluating the temporal and spatial expression of Muc1 at different stages of rat development. In this report, 80 animals were included: 64 rat foetuses at 13, 14, 15, 16, 17, 18, 19 and 20 days of gestation from pregnant females (WKAH/Hok), 8 embryos each stage. Standard immunohistochemistry was performed using anti-MUC1 cytoplasmic tail polyclonal antibody (CT33). The reaction was considered positive when more than 5% of the cells were stained; reaction patterns were: L = linear, membrane, C = cytoplasmic and M = mixed; nuclear staining was also recorded. Intensity was graded as negative (-), low (+), moderate (++) and strong (+++). Muc1 expression was observed with a low intensity on 13th day (13 D) in the stomach, lung and kidney; at 14 d, small intestine and pancreas were also reactive; at 16 D, liver and esophagus and at 18 D, trachea and salivary glands. During the development, intensity increased while the pattern of expression changed: at the first days of gestation, it was predominantly linear and apical while during further development an increase in cytoplasmic expression was observed. Trachea, stomach, kidney and lung epithelia were the more reactive tissues. In specimens belonging to neonates and adults, all tissues analyzed showed similar Muc1 expression. The findings of this study assess that Muc1 is highly expressed in the epithelial rat embryonic development.Facultad de Ciencias VeterinariasCentro de Investigaciones Inmunológicas Básicas y AplicadasFacultad de Ciencias Médicas2010info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf223-228http://sedici.unlp.edu.ar/handle/10915/82440enginfo:eu-repo/semantics/altIdentifier/issn/1121760Xinfo:eu-repo/semantics/altIdentifier/doi/10.4081/ejh.2010.e49info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc/3.0/Creative Commons Attribution-NonCommercial 3.0 Unported (CC BY-NC 3.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:15:31Zoai:sedici.unlp.edu.ar:10915/82440Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:15:31.453SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Immunohistochemical evidence of Muc1 expression during rat embryonic development
title Immunohistochemical evidence of Muc1 expression during rat embryonic development
spellingShingle Immunohistochemical evidence of Muc1 expression during rat embryonic development
Lacunza, Ezequiel
Ciencias Veterinarias
Ciencias Médicas
Embryonic development
Immunohistochemistry
Muc1/MUC1
Rat
title_short Immunohistochemical evidence of Muc1 expression during rat embryonic development
title_full Immunohistochemical evidence of Muc1 expression during rat embryonic development
title_fullStr Immunohistochemical evidence of Muc1 expression during rat embryonic development
title_full_unstemmed Immunohistochemical evidence of Muc1 expression during rat embryonic development
title_sort Immunohistochemical evidence of Muc1 expression during rat embryonic development
dc.creator.none.fl_str_mv Lacunza, Ezequiel
Ferretti, Valeria Alejandra
Barbeito, Claudio Gustavo
Segal-Eiras, Amada
Croce, María Virginia
author Lacunza, Ezequiel
author_facet Lacunza, Ezequiel
Ferretti, Valeria Alejandra
Barbeito, Claudio Gustavo
Segal-Eiras, Amada
Croce, María Virginia
author_role author
author2 Ferretti, Valeria Alejandra
Barbeito, Claudio Gustavo
Segal-Eiras, Amada
Croce, María Virginia
author2_role author
author
author
author
dc.subject.none.fl_str_mv Ciencias Veterinarias
Ciencias Médicas
Embryonic development
Immunohistochemistry
Muc1/MUC1
Rat
topic Ciencias Veterinarias
Ciencias Médicas
Embryonic development
Immunohistochemistry
Muc1/MUC1
Rat
dc.description.none.fl_txt_mv During embryonic development, studies on mouse and human embryos have established that Muc1/MUC1 expression coincides with the onset of epithelial sheet and glandular formation. This study aimed therefore at evaluating the temporal and spatial expression of Muc1 at different stages of rat development. In this report, 80 animals were included: 64 rat foetuses at 13, 14, 15, 16, 17, 18, 19 and 20 days of gestation from pregnant females (WKAH/Hok), 8 embryos each stage. Standard immunohistochemistry was performed using anti-MUC1 cytoplasmic tail polyclonal antibody (CT33). The reaction was considered positive when more than 5% of the cells were stained; reaction patterns were: L = linear, membrane, C = cytoplasmic and M = mixed; nuclear staining was also recorded. Intensity was graded as negative (-), low (+), moderate (++) and strong (+++). Muc1 expression was observed with a low intensity on 13th day (13 D) in the stomach, lung and kidney; at 14 d, small intestine and pancreas were also reactive; at 16 D, liver and esophagus and at 18 D, trachea and salivary glands. During the development, intensity increased while the pattern of expression changed: at the first days of gestation, it was predominantly linear and apical while during further development an increase in cytoplasmic expression was observed. Trachea, stomach, kidney and lung epithelia were the more reactive tissues. In specimens belonging to neonates and adults, all tissues analyzed showed similar Muc1 expression. The findings of this study assess that Muc1 is highly expressed in the epithelial rat embryonic development.
Facultad de Ciencias Veterinarias
Centro de Investigaciones Inmunológicas Básicas y Aplicadas
Facultad de Ciencias Médicas
description During embryonic development, studies on mouse and human embryos have established that Muc1/MUC1 expression coincides with the onset of epithelial sheet and glandular formation. This study aimed therefore at evaluating the temporal and spatial expression of Muc1 at different stages of rat development. In this report, 80 animals were included: 64 rat foetuses at 13, 14, 15, 16, 17, 18, 19 and 20 days of gestation from pregnant females (WKAH/Hok), 8 embryos each stage. Standard immunohistochemistry was performed using anti-MUC1 cytoplasmic tail polyclonal antibody (CT33). The reaction was considered positive when more than 5% of the cells were stained; reaction patterns were: L = linear, membrane, C = cytoplasmic and M = mixed; nuclear staining was also recorded. Intensity was graded as negative (-), low (+), moderate (++) and strong (+++). Muc1 expression was observed with a low intensity on 13th day (13 D) in the stomach, lung and kidney; at 14 d, small intestine and pancreas were also reactive; at 16 D, liver and esophagus and at 18 D, trachea and salivary glands. During the development, intensity increased while the pattern of expression changed: at the first days of gestation, it was predominantly linear and apical while during further development an increase in cytoplasmic expression was observed. Trachea, stomach, kidney and lung epithelia were the more reactive tissues. In specimens belonging to neonates and adults, all tissues analyzed showed similar Muc1 expression. The findings of this study assess that Muc1 is highly expressed in the epithelial rat embryonic development.
publishDate 2010
dc.date.none.fl_str_mv 2010
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
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format article
status_str publishedVersion
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url http://sedici.unlp.edu.ar/handle/10915/82440
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/1121760X
info:eu-repo/semantics/altIdentifier/doi/10.4081/ejh.2010.e49
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc/3.0/
Creative Commons Attribution-NonCommercial 3.0 Unported (CC BY-NC 3.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc/3.0/
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dc.format.none.fl_str_mv application/pdf
223-228
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