Temporal and spatial expression of Muc2 and Muc5ac mucins during rat respiratory and digestive tracts development

Autores
Ferretti, Valeria; Segal Eiras, Amada; Barbeito, Claudio Gustavo; Croce, María Virginia
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Secreted mucins constitute a crucial part of the gel that protects respiratory and digestive epithelia, being MUC2/Muc2 the predominant gel-forming mucin of the intestine while MUC5AC/Muc5ac is one of the gel-forming mucins most expressed at the airways. In this study, we have analyzed Muc2 and Muc5ac during rat development by using immunohistochemistry, Western blotting and RT-PCR. We demonstrated that rat Muc2 was expressed in fetal intestinal goblet cells of surface epithelium of villi and developing Lieberkühn crypts. In neonates and adults, Muc2 was expressed at luminal goblet cells of small and large intestine and at gastric mucous and glandular cells. Muc5ac protein was observed in embryonic gastric and lung samples; expression increased during development and postnatal and adult life. After birth, a low reaction was detected at the tracheal surface epithelium and glands, which increased in adults.
Fil: Ferretti, Valeria. Universidad Nacional de La Plata. Facultad de Ciencias Médicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Segal Eiras, Amada. Universidad Nacional de La Plata. Facultad de Ciencias Médicas; Argentina
Fil: Barbeito, Claudio Gustavo. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Croce, María Virginia. Universidad Nacional de La Plata. Facultad de Ciencias Médicas; Argentina
Materia
Development
Embryology
Muc2
Muc5ac
Rat
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/53699
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Temporal and spatial expression of Muc2 and Muc5ac mucins during rat respiratory and digestive tracts developmentFerretti, ValeriaSegal Eiras, AmadaBarbeito, Claudio GustavoCroce, María VirginiaDevelopmentEmbryologyMuc2Muc5acRathttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Secreted mucins constitute a crucial part of the gel that protects respiratory and digestive epithelia, being MUC2/Muc2 the predominant gel-forming mucin of the intestine while MUC5AC/Muc5ac is one of the gel-forming mucins most expressed at the airways. In this study, we have analyzed Muc2 and Muc5ac during rat development by using immunohistochemistry, Western blotting and RT-PCR. We demonstrated that rat Muc2 was expressed in fetal intestinal goblet cells of surface epithelium of villi and developing Lieberkühn crypts. In neonates and adults, Muc2 was expressed at luminal goblet cells of small and large intestine and at gastric mucous and glandular cells. Muc5ac protein was observed in embryonic gastric and lung samples; expression increased during development and postnatal and adult life. After birth, a low reaction was detected at the tracheal surface epithelium and glands, which increased in adults.Fil: Ferretti, Valeria. Universidad Nacional de La Plata. Facultad de Ciencias Médicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Segal Eiras, Amada. Universidad Nacional de La Plata. Facultad de Ciencias Médicas; ArgentinaFil: Barbeito, Claudio Gustavo. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Croce, María Virginia. Universidad Nacional de La Plata. Facultad de Ciencias Médicas; ArgentinaElsevier2016-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/53699Ferretti, Valeria; Segal Eiras, Amada; Barbeito, Claudio Gustavo; Croce, María Virginia; Temporal and spatial expression of Muc2 and Muc5ac mucins during rat respiratory and digestive tracts development; Elsevier; Research in Veterinary Science; 104; 2-2016; 136-1450034-5288CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.rvsc.2015.12.012info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0034528815301119info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:16:09Zoai:ri.conicet.gov.ar:11336/53699instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:16:09.677CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Temporal and spatial expression of Muc2 and Muc5ac mucins during rat respiratory and digestive tracts development
title Temporal and spatial expression of Muc2 and Muc5ac mucins during rat respiratory and digestive tracts development
spellingShingle Temporal and spatial expression of Muc2 and Muc5ac mucins during rat respiratory and digestive tracts development
Ferretti, Valeria
Development
Embryology
Muc2
Muc5ac
Rat
title_short Temporal and spatial expression of Muc2 and Muc5ac mucins during rat respiratory and digestive tracts development
title_full Temporal and spatial expression of Muc2 and Muc5ac mucins during rat respiratory and digestive tracts development
title_fullStr Temporal and spatial expression of Muc2 and Muc5ac mucins during rat respiratory and digestive tracts development
title_full_unstemmed Temporal and spatial expression of Muc2 and Muc5ac mucins during rat respiratory and digestive tracts development
title_sort Temporal and spatial expression of Muc2 and Muc5ac mucins during rat respiratory and digestive tracts development
dc.creator.none.fl_str_mv Ferretti, Valeria
Segal Eiras, Amada
Barbeito, Claudio Gustavo
Croce, María Virginia
author Ferretti, Valeria
author_facet Ferretti, Valeria
Segal Eiras, Amada
Barbeito, Claudio Gustavo
Croce, María Virginia
author_role author
author2 Segal Eiras, Amada
Barbeito, Claudio Gustavo
Croce, María Virginia
author2_role author
author
author
dc.subject.none.fl_str_mv Development
Embryology
Muc2
Muc5ac
Rat
topic Development
Embryology
Muc2
Muc5ac
Rat
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Secreted mucins constitute a crucial part of the gel that protects respiratory and digestive epithelia, being MUC2/Muc2 the predominant gel-forming mucin of the intestine while MUC5AC/Muc5ac is one of the gel-forming mucins most expressed at the airways. In this study, we have analyzed Muc2 and Muc5ac during rat development by using immunohistochemistry, Western blotting and RT-PCR. We demonstrated that rat Muc2 was expressed in fetal intestinal goblet cells of surface epithelium of villi and developing Lieberkühn crypts. In neonates and adults, Muc2 was expressed at luminal goblet cells of small and large intestine and at gastric mucous and glandular cells. Muc5ac protein was observed in embryonic gastric and lung samples; expression increased during development and postnatal and adult life. After birth, a low reaction was detected at the tracheal surface epithelium and glands, which increased in adults.
Fil: Ferretti, Valeria. Universidad Nacional de La Plata. Facultad de Ciencias Médicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Segal Eiras, Amada. Universidad Nacional de La Plata. Facultad de Ciencias Médicas; Argentina
Fil: Barbeito, Claudio Gustavo. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Croce, María Virginia. Universidad Nacional de La Plata. Facultad de Ciencias Médicas; Argentina
description Secreted mucins constitute a crucial part of the gel that protects respiratory and digestive epithelia, being MUC2/Muc2 the predominant gel-forming mucin of the intestine while MUC5AC/Muc5ac is one of the gel-forming mucins most expressed at the airways. In this study, we have analyzed Muc2 and Muc5ac during rat development by using immunohistochemistry, Western blotting and RT-PCR. We demonstrated that rat Muc2 was expressed in fetal intestinal goblet cells of surface epithelium of villi and developing Lieberkühn crypts. In neonates and adults, Muc2 was expressed at luminal goblet cells of small and large intestine and at gastric mucous and glandular cells. Muc5ac protein was observed in embryonic gastric and lung samples; expression increased during development and postnatal and adult life. After birth, a low reaction was detected at the tracheal surface epithelium and glands, which increased in adults.
publishDate 2016
dc.date.none.fl_str_mv 2016-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/53699
Ferretti, Valeria; Segal Eiras, Amada; Barbeito, Claudio Gustavo; Croce, María Virginia; Temporal and spatial expression of Muc2 and Muc5ac mucins during rat respiratory and digestive tracts development; Elsevier; Research in Veterinary Science; 104; 2-2016; 136-145
0034-5288
CONICET Digital
CONICET
url http://hdl.handle.net/11336/53699
identifier_str_mv Ferretti, Valeria; Segal Eiras, Amada; Barbeito, Claudio Gustavo; Croce, María Virginia; Temporal and spatial expression of Muc2 and Muc5ac mucins during rat respiratory and digestive tracts development; Elsevier; Research in Veterinary Science; 104; 2-2016; 136-145
0034-5288
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.rvsc.2015.12.012
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0034528815301119
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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