Characterization of novel <i>Trichoderma</i> spp. isolates as a search for effective biocontrollers of fungal diseases of economically important crops in Argentina

Autores
Consolo, Verónica Fabiana; Mónaco, Cecilia Inés; Cordo, Cristina Alicia; Salerno, Graciela Lidia
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Monoconidial cultures of 33 isolates of Trichoderma from Buenos Aires Province, Argentina were characterized on the basis of twenty eight morphological, physiological and biochemical features. All of them were screened for proteinase, endochitinase and β-1,3 glucanase activity. Universally primed PCR (UP-PCR) and inter-simple sequence repeat (ISSR) techniques were used to examine the genetic variability among isolates, which resulted in 127 bands for the total number of isolates. These results were subjected to numerical analysis revealing 20 haplotypes grouped in five clusters. The ability of Trichoderma isolates to antogonize soil-borne fungal plant pathogens using a dual culture assay was done against five fungal species: Alternaria sp., Bipolaris sorokiniana, Fusarium graminearum, F. solani, and Pyricularia oryzae. The highest inhibition values (85% RI) were obtained against B. sorokiniana and P. oryzae. Three isolates of T. harzianum named as FCCT2, FCCT3 and FCCT9 were capable of causing a high growth inhibition on four of the fungal species assayed, which was in agreement with their higher extracellular hydrolytic activity. Our results suggest that these isolates have the potential to be effective agents for biocontrol of cereal and tomato fungal pathogens.
Facultad de Ciencias Agrarias y Forestales
Centro de Investigaciones en Fitopatología
Materia
Ciencias Agrarias
Ciencias Naturales
Biocontrol
Characterization
Phytopathogens
Trichoderma spp.
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/131623

id SEDICI_91fa5f4cb941e35d2697099c1ea0b9ce
oai_identifier_str oai:sedici.unlp.edu.ar:10915/131623
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Characterization of novel <i>Trichoderma</i> spp. isolates as a search for effective biocontrollers of fungal diseases of economically important crops in ArgentinaConsolo, Verónica FabianaMónaco, Cecilia InésCordo, Cristina AliciaSalerno, Graciela LidiaCiencias AgrariasCiencias NaturalesBiocontrolCharacterizationPhytopathogensTrichoderma spp.Monoconidial cultures of 33 isolates of <i>Trichoderma</i> from Buenos Aires Province, Argentina were characterized on the basis of twenty eight morphological, physiological and biochemical features. All of them were screened for proteinase, endochitinase and β-1,3 glucanase activity. Universally primed PCR (UP-PCR) and inter-simple sequence repeat (ISSR) techniques were used to examine the genetic variability among isolates, which resulted in 127 bands for the total number of isolates. These results were subjected to numerical analysis revealing 20 haplotypes grouped in five clusters. The ability of <i>Trichoderma</i> isolates to antogonize soil-borne fungal plant pathogens using a dual culture assay was done against five fungal species: <i>Alternaria</i> sp., <i>Bipolaris sorokiniana</i>, <i>Fusarium graminearum</i>, <i>F. solani</i>, and <i>Pyricularia oryzae</i>. The highest inhibition values (85% RI) were obtained against <i>B. sorokiniana</i> and <i>P. oryzae</i>. Three isolates of <i>T. harzianum</i> named as FCCT2, FCCT3 and FCCT9 were capable of causing a high growth inhibition on four of the fungal species assayed, which was in agreement with their higher extracellular hydrolytic activity. Our results suggest that these isolates have the potential to be effective agents for biocontrol of cereal and tomato fungal pathogens.Facultad de Ciencias Agrarias y ForestalesCentro de Investigaciones en Fitopatología2012-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf1389-1398http://sedici.unlp.edu.ar/handle/10915/131623enginfo:eu-repo/semantics/altIdentifier/issn/1573-0972info:eu-repo/semantics/altIdentifier/issn/0959-3993info:eu-repo/semantics/altIdentifier/doi/10.1007/s11274-011-0938-5info:eu-repo/semantics/altIdentifier/pmid/22805919info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:31:47Zoai:sedici.unlp.edu.ar:10915/131623Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:31:47.51SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Characterization of novel <i>Trichoderma</i> spp. isolates as a search for effective biocontrollers of fungal diseases of economically important crops in Argentina
title Characterization of novel <i>Trichoderma</i> spp. isolates as a search for effective biocontrollers of fungal diseases of economically important crops in Argentina
spellingShingle Characterization of novel <i>Trichoderma</i> spp. isolates as a search for effective biocontrollers of fungal diseases of economically important crops in Argentina
Consolo, Verónica Fabiana
Ciencias Agrarias
Ciencias Naturales
Biocontrol
Characterization
Phytopathogens
Trichoderma spp.
title_short Characterization of novel <i>Trichoderma</i> spp. isolates as a search for effective biocontrollers of fungal diseases of economically important crops in Argentina
title_full Characterization of novel <i>Trichoderma</i> spp. isolates as a search for effective biocontrollers of fungal diseases of economically important crops in Argentina
title_fullStr Characterization of novel <i>Trichoderma</i> spp. isolates as a search for effective biocontrollers of fungal diseases of economically important crops in Argentina
title_full_unstemmed Characterization of novel <i>Trichoderma</i> spp. isolates as a search for effective biocontrollers of fungal diseases of economically important crops in Argentina
title_sort Characterization of novel <i>Trichoderma</i> spp. isolates as a search for effective biocontrollers of fungal diseases of economically important crops in Argentina
dc.creator.none.fl_str_mv Consolo, Verónica Fabiana
Mónaco, Cecilia Inés
Cordo, Cristina Alicia
Salerno, Graciela Lidia
author Consolo, Verónica Fabiana
author_facet Consolo, Verónica Fabiana
Mónaco, Cecilia Inés
Cordo, Cristina Alicia
Salerno, Graciela Lidia
author_role author
author2 Mónaco, Cecilia Inés
Cordo, Cristina Alicia
Salerno, Graciela Lidia
author2_role author
author
author
dc.subject.none.fl_str_mv Ciencias Agrarias
Ciencias Naturales
Biocontrol
Characterization
Phytopathogens
Trichoderma spp.
topic Ciencias Agrarias
Ciencias Naturales
Biocontrol
Characterization
Phytopathogens
Trichoderma spp.
dc.description.none.fl_txt_mv Monoconidial cultures of 33 isolates of <i>Trichoderma</i> from Buenos Aires Province, Argentina were characterized on the basis of twenty eight morphological, physiological and biochemical features. All of them were screened for proteinase, endochitinase and β-1,3 glucanase activity. Universally primed PCR (UP-PCR) and inter-simple sequence repeat (ISSR) techniques were used to examine the genetic variability among isolates, which resulted in 127 bands for the total number of isolates. These results were subjected to numerical analysis revealing 20 haplotypes grouped in five clusters. The ability of <i>Trichoderma</i> isolates to antogonize soil-borne fungal plant pathogens using a dual culture assay was done against five fungal species: <i>Alternaria</i> sp., <i>Bipolaris sorokiniana</i>, <i>Fusarium graminearum</i>, <i>F. solani</i>, and <i>Pyricularia oryzae</i>. The highest inhibition values (85% RI) were obtained against <i>B. sorokiniana</i> and <i>P. oryzae</i>. Three isolates of <i>T. harzianum</i> named as FCCT2, FCCT3 and FCCT9 were capable of causing a high growth inhibition on four of the fungal species assayed, which was in agreement with their higher extracellular hydrolytic activity. Our results suggest that these isolates have the potential to be effective agents for biocontrol of cereal and tomato fungal pathogens.
Facultad de Ciencias Agrarias y Forestales
Centro de Investigaciones en Fitopatología
description Monoconidial cultures of 33 isolates of <i>Trichoderma</i> from Buenos Aires Province, Argentina were characterized on the basis of twenty eight morphological, physiological and biochemical features. All of them were screened for proteinase, endochitinase and β-1,3 glucanase activity. Universally primed PCR (UP-PCR) and inter-simple sequence repeat (ISSR) techniques were used to examine the genetic variability among isolates, which resulted in 127 bands for the total number of isolates. These results were subjected to numerical analysis revealing 20 haplotypes grouped in five clusters. The ability of <i>Trichoderma</i> isolates to antogonize soil-borne fungal plant pathogens using a dual culture assay was done against five fungal species: <i>Alternaria</i> sp., <i>Bipolaris sorokiniana</i>, <i>Fusarium graminearum</i>, <i>F. solani</i>, and <i>Pyricularia oryzae</i>. The highest inhibition values (85% RI) were obtained against <i>B. sorokiniana</i> and <i>P. oryzae</i>. Three isolates of <i>T. harzianum</i> named as FCCT2, FCCT3 and FCCT9 were capable of causing a high growth inhibition on four of the fungal species assayed, which was in agreement with their higher extracellular hydrolytic activity. Our results suggest that these isolates have the potential to be effective agents for biocontrol of cereal and tomato fungal pathogens.
publishDate 2012
dc.date.none.fl_str_mv 2012-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/131623
url http://sedici.unlp.edu.ar/handle/10915/131623
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/1573-0972
info:eu-repo/semantics/altIdentifier/issn/0959-3993
info:eu-repo/semantics/altIdentifier/doi/10.1007/s11274-011-0938-5
info:eu-repo/semantics/altIdentifier/pmid/22805919
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
1389-1398
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
_version_ 1844616197578948608
score 13.070432