Characterization of novel <i>Trichoderma</i> spp. isolates as a search for effective biocontrollers of fungal diseases of economically important crops in Argentina
- Autores
- Consolo, Verónica Fabiana; Mónaco, Cecilia Inés; Cordo, Cristina Alicia; Salerno, Graciela Lidia
- Año de publicación
- 2012
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Monoconidial cultures of 33 isolates of Trichoderma from Buenos Aires Province, Argentina were characterized on the basis of twenty eight morphological, physiological and biochemical features. All of them were screened for proteinase, endochitinase and β-1,3 glucanase activity. Universally primed PCR (UP-PCR) and inter-simple sequence repeat (ISSR) techniques were used to examine the genetic variability among isolates, which resulted in 127 bands for the total number of isolates. These results were subjected to numerical analysis revealing 20 haplotypes grouped in five clusters. The ability of Trichoderma isolates to antogonize soil-borne fungal plant pathogens using a dual culture assay was done against five fungal species: Alternaria sp., Bipolaris sorokiniana, Fusarium graminearum, F. solani, and Pyricularia oryzae. The highest inhibition values (85% RI) were obtained against B. sorokiniana and P. oryzae. Three isolates of T. harzianum named as FCCT2, FCCT3 and FCCT9 were capable of causing a high growth inhibition on four of the fungal species assayed, which was in agreement with their higher extracellular hydrolytic activity. Our results suggest that these isolates have the potential to be effective agents for biocontrol of cereal and tomato fungal pathogens.
Facultad de Ciencias Agrarias y Forestales
Centro de Investigaciones en Fitopatología - Materia
-
Ciencias Agrarias
Ciencias Naturales
Biocontrol
Characterization
Phytopathogens
Trichoderma spp. - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by-nc-sa/4.0/
- Repositorio
- Institución
- Universidad Nacional de La Plata
- OAI Identificador
- oai:sedici.unlp.edu.ar:10915/131623
Ver los metadatos del registro completo
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Characterization of novel <i>Trichoderma</i> spp. isolates as a search for effective biocontrollers of fungal diseases of economically important crops in ArgentinaConsolo, Verónica FabianaMónaco, Cecilia InésCordo, Cristina AliciaSalerno, Graciela LidiaCiencias AgrariasCiencias NaturalesBiocontrolCharacterizationPhytopathogensTrichoderma spp.Monoconidial cultures of 33 isolates of <i>Trichoderma</i> from Buenos Aires Province, Argentina were characterized on the basis of twenty eight morphological, physiological and biochemical features. All of them were screened for proteinase, endochitinase and β-1,3 glucanase activity. Universally primed PCR (UP-PCR) and inter-simple sequence repeat (ISSR) techniques were used to examine the genetic variability among isolates, which resulted in 127 bands for the total number of isolates. These results were subjected to numerical analysis revealing 20 haplotypes grouped in five clusters. The ability of <i>Trichoderma</i> isolates to antogonize soil-borne fungal plant pathogens using a dual culture assay was done against five fungal species: <i>Alternaria</i> sp., <i>Bipolaris sorokiniana</i>, <i>Fusarium graminearum</i>, <i>F. solani</i>, and <i>Pyricularia oryzae</i>. The highest inhibition values (85% RI) were obtained against <i>B. sorokiniana</i> and <i>P. oryzae</i>. Three isolates of <i>T. harzianum</i> named as FCCT2, FCCT3 and FCCT9 were capable of causing a high growth inhibition on four of the fungal species assayed, which was in agreement with their higher extracellular hydrolytic activity. Our results suggest that these isolates have the potential to be effective agents for biocontrol of cereal and tomato fungal pathogens.Facultad de Ciencias Agrarias y ForestalesCentro de Investigaciones en Fitopatología2012-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf1389-1398http://sedici.unlp.edu.ar/handle/10915/131623enginfo:eu-repo/semantics/altIdentifier/issn/1573-0972info:eu-repo/semantics/altIdentifier/issn/0959-3993info:eu-repo/semantics/altIdentifier/doi/10.1007/s11274-011-0938-5info:eu-repo/semantics/altIdentifier/pmid/22805919info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:31:47Zoai:sedici.unlp.edu.ar:10915/131623Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:31:47.51SEDICI (UNLP) - Universidad Nacional de La Platafalse |
dc.title.none.fl_str_mv |
Characterization of novel <i>Trichoderma</i> spp. isolates as a search for effective biocontrollers of fungal diseases of economically important crops in Argentina |
title |
Characterization of novel <i>Trichoderma</i> spp. isolates as a search for effective biocontrollers of fungal diseases of economically important crops in Argentina |
spellingShingle |
Characterization of novel <i>Trichoderma</i> spp. isolates as a search for effective biocontrollers of fungal diseases of economically important crops in Argentina Consolo, Verónica Fabiana Ciencias Agrarias Ciencias Naturales Biocontrol Characterization Phytopathogens Trichoderma spp. |
title_short |
Characterization of novel <i>Trichoderma</i> spp. isolates as a search for effective biocontrollers of fungal diseases of economically important crops in Argentina |
title_full |
Characterization of novel <i>Trichoderma</i> spp. isolates as a search for effective biocontrollers of fungal diseases of economically important crops in Argentina |
title_fullStr |
Characterization of novel <i>Trichoderma</i> spp. isolates as a search for effective biocontrollers of fungal diseases of economically important crops in Argentina |
title_full_unstemmed |
Characterization of novel <i>Trichoderma</i> spp. isolates as a search for effective biocontrollers of fungal diseases of economically important crops in Argentina |
title_sort |
Characterization of novel <i>Trichoderma</i> spp. isolates as a search for effective biocontrollers of fungal diseases of economically important crops in Argentina |
dc.creator.none.fl_str_mv |
Consolo, Verónica Fabiana Mónaco, Cecilia Inés Cordo, Cristina Alicia Salerno, Graciela Lidia |
author |
Consolo, Verónica Fabiana |
author_facet |
Consolo, Verónica Fabiana Mónaco, Cecilia Inés Cordo, Cristina Alicia Salerno, Graciela Lidia |
author_role |
author |
author2 |
Mónaco, Cecilia Inés Cordo, Cristina Alicia Salerno, Graciela Lidia |
author2_role |
author author author |
dc.subject.none.fl_str_mv |
Ciencias Agrarias Ciencias Naturales Biocontrol Characterization Phytopathogens Trichoderma spp. |
topic |
Ciencias Agrarias Ciencias Naturales Biocontrol Characterization Phytopathogens Trichoderma spp. |
dc.description.none.fl_txt_mv |
Monoconidial cultures of 33 isolates of <i>Trichoderma</i> from Buenos Aires Province, Argentina were characterized on the basis of twenty eight morphological, physiological and biochemical features. All of them were screened for proteinase, endochitinase and β-1,3 glucanase activity. Universally primed PCR (UP-PCR) and inter-simple sequence repeat (ISSR) techniques were used to examine the genetic variability among isolates, which resulted in 127 bands for the total number of isolates. These results were subjected to numerical analysis revealing 20 haplotypes grouped in five clusters. The ability of <i>Trichoderma</i> isolates to antogonize soil-borne fungal plant pathogens using a dual culture assay was done against five fungal species: <i>Alternaria</i> sp., <i>Bipolaris sorokiniana</i>, <i>Fusarium graminearum</i>, <i>F. solani</i>, and <i>Pyricularia oryzae</i>. The highest inhibition values (85% RI) were obtained against <i>B. sorokiniana</i> and <i>P. oryzae</i>. Three isolates of <i>T. harzianum</i> named as FCCT2, FCCT3 and FCCT9 were capable of causing a high growth inhibition on four of the fungal species assayed, which was in agreement with their higher extracellular hydrolytic activity. Our results suggest that these isolates have the potential to be effective agents for biocontrol of cereal and tomato fungal pathogens. Facultad de Ciencias Agrarias y Forestales Centro de Investigaciones en Fitopatología |
description |
Monoconidial cultures of 33 isolates of <i>Trichoderma</i> from Buenos Aires Province, Argentina were characterized on the basis of twenty eight morphological, physiological and biochemical features. All of them were screened for proteinase, endochitinase and β-1,3 glucanase activity. Universally primed PCR (UP-PCR) and inter-simple sequence repeat (ISSR) techniques were used to examine the genetic variability among isolates, which resulted in 127 bands for the total number of isolates. These results were subjected to numerical analysis revealing 20 haplotypes grouped in five clusters. The ability of <i>Trichoderma</i> isolates to antogonize soil-borne fungal plant pathogens using a dual culture assay was done against five fungal species: <i>Alternaria</i> sp., <i>Bipolaris sorokiniana</i>, <i>Fusarium graminearum</i>, <i>F. solani</i>, and <i>Pyricularia oryzae</i>. The highest inhibition values (85% RI) were obtained against <i>B. sorokiniana</i> and <i>P. oryzae</i>. Three isolates of <i>T. harzianum</i> named as FCCT2, FCCT3 and FCCT9 were capable of causing a high growth inhibition on four of the fungal species assayed, which was in agreement with their higher extracellular hydrolytic activity. Our results suggest that these isolates have the potential to be effective agents for biocontrol of cereal and tomato fungal pathogens. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-04 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Articulo http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
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http://sedici.unlp.edu.ar/handle/10915/131623 |
url |
http://sedici.unlp.edu.ar/handle/10915/131623 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
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dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
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openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
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