Characterization of novel Trichoderma spp. isolates as a search for effective biocontrollers of fungal diseases of economically important crops in Argentina

Autores
Consolo, Verónica Fabiana; Mónaco, Cecilia Inés; Cordo, Cristina Alicia; Salerno, Graciela Lidia
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión enviada
Descripción
Monoconidial cultures of 33 isolates ofTrichodermafrom Buenos Aires Province, Argentina were characterized on the basis of twenty eight morphological, physiological and biochemical features. All of them were screened for proteinase, endochitinase andβ-1,3 glucanase activity. Universally primed PCR (UP-PCR) and inter-simple sequence repeat (ISSR) techniques were used to examine the genetic variability among isolates, which resulted in 127 bands for the total number of isolates. These results were subjected to numerical analysis revealing 20 haplotypes grouped in five clusters. The ability ofTrichodermaisolates to antogonize soil-borne fungal plant pathogens using a dual culture assay was done against five fungal species:Alternariasp.,Bipolaris sorokiniana, Fusarium graminearum,F. solani, andPyricularia oryzae.The highest inhibition values (85% RI) were obtained againstB. sorokinianaandP. oryzae.Three isolates ofT. harzianumnamed as FCCT2, FCCT3 and FCCT9 were capable of causing a high growth inhibition on four of the fungal species assayed, which was in agreement with their higher extracellular hydrolytic activity. Our results suggest that these isolates have the potential to be effective agents for biocontrol of cereal and tomato fungal pathogens.
Materia
Ciencias Naturales y Exactas
biocontrol
characterization
phytopathogens
Trichoderma spp.
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-nd/4.0/
Repositorio
CIC Digital (CICBA)
Institución
Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
OAI Identificador
oai:digital.cic.gba.gob.ar:11746/5624

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oai_identifier_str oai:digital.cic.gba.gob.ar:11746/5624
network_acronym_str CICBA
repository_id_str 9441
network_name_str CIC Digital (CICBA)
spelling Characterization of novel Trichoderma spp. isolates as a search for effective biocontrollers of fungal diseases of economically important crops in ArgentinaConsolo, Verónica FabianaMónaco, Cecilia InésCordo, Cristina AliciaSalerno, Graciela LidiaCiencias Naturales y ExactasbiocontrolcharacterizationphytopathogensTrichoderma spp.Monoconidial cultures of 33 isolates of<em>Trichoderma</em>from Buenos Aires Province, Argentina were characterized on the basis of twenty eight morphological, physiological and biochemical features. All of them were screened for proteinase, endochitinase and<em>β</em>-1,3 glucanase activity. Universally primed PCR (UP-PCR) and inter-simple sequence repeat (ISSR) techniques were used to examine the genetic variability among isolates, which resulted in 127 bands for the total number of isolates. These results were subjected to numerical analysis revealing 20 haplotypes grouped in five clusters. The ability of<em>Trichoderma</em>isolates to antogonize soil-borne fungal plant pathogens using a dual culture assay was done against five fungal species:<em>Alternaria</em>sp.,<em>Bipolaris sorokiniana, Fusarium graminearum</em>,<em>F. solani</em>, and<em>Pyricularia oryzae.</em>The highest inhibition values (85% RI) were obtained against<em>B. sorokiniana</em>and<em>P. oryzae.</em>Three isolates of<em>T. harzianum</em>named as FCCT2, FCCT3 and FCCT9 were capable of causing a high growth inhibition on four of the fungal species assayed, which was in agreement with their higher extracellular hydrolytic activity. Our results suggest that these isolates have the potential to be effective agents for biocontrol of cereal and tomato fungal pathogens.2012info:eu-repo/semantics/articleinfo:eu-repo/semantics/submittedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://digital.cic.gba.gob.ar/handle/11746/5624enginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/4.0/reponame:CIC Digital (CICBA)instname:Comisión de Investigaciones Científicas de la Provincia de Buenos Airesinstacron:CICBA2025-09-29T13:40:21Zoai:digital.cic.gba.gob.ar:11746/5624Institucionalhttp://digital.cic.gba.gob.arOrganismo científico-tecnológicoNo correspondehttp://digital.cic.gba.gob.ar/oai/snrdmarisa.degiusti@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:94412025-09-29 13:40:21.232CIC Digital (CICBA) - Comisión de Investigaciones Científicas de la Provincia de Buenos Airesfalse
dc.title.none.fl_str_mv Characterization of novel Trichoderma spp. isolates as a search for effective biocontrollers of fungal diseases of economically important crops in Argentina
title Characterization of novel Trichoderma spp. isolates as a search for effective biocontrollers of fungal diseases of economically important crops in Argentina
spellingShingle Characterization of novel Trichoderma spp. isolates as a search for effective biocontrollers of fungal diseases of economically important crops in Argentina
Consolo, Verónica Fabiana
Ciencias Naturales y Exactas
biocontrol
characterization
phytopathogens
Trichoderma spp.
title_short Characterization of novel Trichoderma spp. isolates as a search for effective biocontrollers of fungal diseases of economically important crops in Argentina
title_full Characterization of novel Trichoderma spp. isolates as a search for effective biocontrollers of fungal diseases of economically important crops in Argentina
title_fullStr Characterization of novel Trichoderma spp. isolates as a search for effective biocontrollers of fungal diseases of economically important crops in Argentina
title_full_unstemmed Characterization of novel Trichoderma spp. isolates as a search for effective biocontrollers of fungal diseases of economically important crops in Argentina
title_sort Characterization of novel Trichoderma spp. isolates as a search for effective biocontrollers of fungal diseases of economically important crops in Argentina
dc.creator.none.fl_str_mv Consolo, Verónica Fabiana
Mónaco, Cecilia Inés
Cordo, Cristina Alicia
Salerno, Graciela Lidia
author Consolo, Verónica Fabiana
author_facet Consolo, Verónica Fabiana
Mónaco, Cecilia Inés
Cordo, Cristina Alicia
Salerno, Graciela Lidia
author_role author
author2 Mónaco, Cecilia Inés
Cordo, Cristina Alicia
Salerno, Graciela Lidia
author2_role author
author
author
dc.subject.none.fl_str_mv Ciencias Naturales y Exactas
biocontrol
characterization
phytopathogens
Trichoderma spp.
topic Ciencias Naturales y Exactas
biocontrol
characterization
phytopathogens
Trichoderma spp.
dc.description.none.fl_txt_mv Monoconidial cultures of 33 isolates of<em>Trichoderma</em>from Buenos Aires Province, Argentina were characterized on the basis of twenty eight morphological, physiological and biochemical features. All of them were screened for proteinase, endochitinase and<em>β</em>-1,3 glucanase activity. Universally primed PCR (UP-PCR) and inter-simple sequence repeat (ISSR) techniques were used to examine the genetic variability among isolates, which resulted in 127 bands for the total number of isolates. These results were subjected to numerical analysis revealing 20 haplotypes grouped in five clusters. The ability of<em>Trichoderma</em>isolates to antogonize soil-borne fungal plant pathogens using a dual culture assay was done against five fungal species:<em>Alternaria</em>sp.,<em>Bipolaris sorokiniana, Fusarium graminearum</em>,<em>F. solani</em>, and<em>Pyricularia oryzae.</em>The highest inhibition values (85% RI) were obtained against<em>B. sorokiniana</em>and<em>P. oryzae.</em>Three isolates of<em>T. harzianum</em>named as FCCT2, FCCT3 and FCCT9 were capable of causing a high growth inhibition on four of the fungal species assayed, which was in agreement with their higher extracellular hydrolytic activity. Our results suggest that these isolates have the potential to be effective agents for biocontrol of cereal and tomato fungal pathogens.
description Monoconidial cultures of 33 isolates of<em>Trichoderma</em>from Buenos Aires Province, Argentina were characterized on the basis of twenty eight morphological, physiological and biochemical features. All of them were screened for proteinase, endochitinase and<em>β</em>-1,3 glucanase activity. Universally primed PCR (UP-PCR) and inter-simple sequence repeat (ISSR) techniques were used to examine the genetic variability among isolates, which resulted in 127 bands for the total number of isolates. These results were subjected to numerical analysis revealing 20 haplotypes grouped in five clusters. The ability of<em>Trichoderma</em>isolates to antogonize soil-borne fungal plant pathogens using a dual culture assay was done against five fungal species:<em>Alternaria</em>sp.,<em>Bipolaris sorokiniana, Fusarium graminearum</em>,<em>F. solani</em>, and<em>Pyricularia oryzae.</em>The highest inhibition values (85% RI) were obtained against<em>B. sorokiniana</em>and<em>P. oryzae.</em>Three isolates of<em>T. harzianum</em>named as FCCT2, FCCT3 and FCCT9 were capable of causing a high growth inhibition on four of the fungal species assayed, which was in agreement with their higher extracellular hydrolytic activity. Our results suggest that these isolates have the potential to be effective agents for biocontrol of cereal and tomato fungal pathogens.
publishDate 2012
dc.date.none.fl_str_mv 2012
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/submittedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str submittedVersion
dc.identifier.none.fl_str_mv https://digital.cic.gba.gob.ar/handle/11746/5624
url https://digital.cic.gba.gob.ar/handle/11746/5624
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:CIC Digital (CICBA)
instname:Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
instacron:CICBA
reponame_str CIC Digital (CICBA)
collection CIC Digital (CICBA)
instname_str Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
instacron_str CICBA
institution CICBA
repository.name.fl_str_mv CIC Digital (CICBA) - Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
repository.mail.fl_str_mv marisa.degiusti@sedici.unlp.edu.ar
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