Characterization of novel Trichoderma spp. isolates as a search for effective biocontrollers of fungal diseases of economically important crops in Argentina
- Autores
- Consolo, Verónica Fabiana; Mónaco, Cecilia Inés; Cordo, Cristina Alicia; Salerno, Graciela Lidia
- Año de publicación
- 2012
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión enviada
- Descripción
- Monoconidial cultures of 33 isolates ofTrichodermafrom Buenos Aires Province, Argentina were characterized on the basis of twenty eight morphological, physiological and biochemical features. All of them were screened for proteinase, endochitinase andβ-1,3 glucanase activity. Universally primed PCR (UP-PCR) and inter-simple sequence repeat (ISSR) techniques were used to examine the genetic variability among isolates, which resulted in 127 bands for the total number of isolates. These results were subjected to numerical analysis revealing 20 haplotypes grouped in five clusters. The ability ofTrichodermaisolates to antogonize soil-borne fungal plant pathogens using a dual culture assay was done against five fungal species:Alternariasp.,Bipolaris sorokiniana, Fusarium graminearum,F. solani, andPyricularia oryzae.The highest inhibition values (85% RI) were obtained againstB. sorokinianaandP. oryzae.Three isolates ofT. harzianumnamed as FCCT2, FCCT3 and FCCT9 were capable of causing a high growth inhibition on four of the fungal species assayed, which was in agreement with their higher extracellular hydrolytic activity. Our results suggest that these isolates have the potential to be effective agents for biocontrol of cereal and tomato fungal pathogens.
- Materia
-
Ciencias Naturales y Exactas
biocontrol
characterization
phytopathogens
Trichoderma spp. - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by-nc-nd/4.0/
- Repositorio
- Institución
- Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
- OAI Identificador
- oai:digital.cic.gba.gob.ar:11746/5624
Ver los metadatos del registro completo
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Characterization of novel Trichoderma spp. isolates as a search for effective biocontrollers of fungal diseases of economically important crops in ArgentinaConsolo, Verónica FabianaMónaco, Cecilia InésCordo, Cristina AliciaSalerno, Graciela LidiaCiencias Naturales y ExactasbiocontrolcharacterizationphytopathogensTrichoderma spp.Monoconidial cultures of 33 isolates of<em>Trichoderma</em>from Buenos Aires Province, Argentina were characterized on the basis of twenty eight morphological, physiological and biochemical features. All of them were screened for proteinase, endochitinase and<em>β</em>-1,3 glucanase activity. Universally primed PCR (UP-PCR) and inter-simple sequence repeat (ISSR) techniques were used to examine the genetic variability among isolates, which resulted in 127 bands for the total number of isolates. These results were subjected to numerical analysis revealing 20 haplotypes grouped in five clusters. The ability of<em>Trichoderma</em>isolates to antogonize soil-borne fungal plant pathogens using a dual culture assay was done against five fungal species:<em>Alternaria</em>sp.,<em>Bipolaris sorokiniana, Fusarium graminearum</em>,<em>F. solani</em>, and<em>Pyricularia oryzae.</em>The highest inhibition values (85% RI) were obtained against<em>B. sorokiniana</em>and<em>P. oryzae.</em>Three isolates of<em>T. harzianum</em>named as FCCT2, FCCT3 and FCCT9 were capable of causing a high growth inhibition on four of the fungal species assayed, which was in agreement with their higher extracellular hydrolytic activity. Our results suggest that these isolates have the potential to be effective agents for biocontrol of cereal and tomato fungal pathogens.2012info:eu-repo/semantics/articleinfo:eu-repo/semantics/submittedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://digital.cic.gba.gob.ar/handle/11746/5624enginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/4.0/reponame:CIC Digital (CICBA)instname:Comisión de Investigaciones Científicas de la Provincia de Buenos Airesinstacron:CICBA2025-09-29T13:40:21Zoai:digital.cic.gba.gob.ar:11746/5624Institucionalhttp://digital.cic.gba.gob.arOrganismo científico-tecnológicoNo correspondehttp://digital.cic.gba.gob.ar/oai/snrdmarisa.degiusti@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:94412025-09-29 13:40:21.232CIC Digital (CICBA) - Comisión de Investigaciones Científicas de la Provincia de Buenos Airesfalse |
dc.title.none.fl_str_mv |
Characterization of novel Trichoderma spp. isolates as a search for effective biocontrollers of fungal diseases of economically important crops in Argentina |
title |
Characterization of novel Trichoderma spp. isolates as a search for effective biocontrollers of fungal diseases of economically important crops in Argentina |
spellingShingle |
Characterization of novel Trichoderma spp. isolates as a search for effective biocontrollers of fungal diseases of economically important crops in Argentina Consolo, Verónica Fabiana Ciencias Naturales y Exactas biocontrol characterization phytopathogens Trichoderma spp. |
title_short |
Characterization of novel Trichoderma spp. isolates as a search for effective biocontrollers of fungal diseases of economically important crops in Argentina |
title_full |
Characterization of novel Trichoderma spp. isolates as a search for effective biocontrollers of fungal diseases of economically important crops in Argentina |
title_fullStr |
Characterization of novel Trichoderma spp. isolates as a search for effective biocontrollers of fungal diseases of economically important crops in Argentina |
title_full_unstemmed |
Characterization of novel Trichoderma spp. isolates as a search for effective biocontrollers of fungal diseases of economically important crops in Argentina |
title_sort |
Characterization of novel Trichoderma spp. isolates as a search for effective biocontrollers of fungal diseases of economically important crops in Argentina |
dc.creator.none.fl_str_mv |
Consolo, Verónica Fabiana Mónaco, Cecilia Inés Cordo, Cristina Alicia Salerno, Graciela Lidia |
author |
Consolo, Verónica Fabiana |
author_facet |
Consolo, Verónica Fabiana Mónaco, Cecilia Inés Cordo, Cristina Alicia Salerno, Graciela Lidia |
author_role |
author |
author2 |
Mónaco, Cecilia Inés Cordo, Cristina Alicia Salerno, Graciela Lidia |
author2_role |
author author author |
dc.subject.none.fl_str_mv |
Ciencias Naturales y Exactas biocontrol characterization phytopathogens Trichoderma spp. |
topic |
Ciencias Naturales y Exactas biocontrol characterization phytopathogens Trichoderma spp. |
dc.description.none.fl_txt_mv |
Monoconidial cultures of 33 isolates of<em>Trichoderma</em>from Buenos Aires Province, Argentina were characterized on the basis of twenty eight morphological, physiological and biochemical features. All of them were screened for proteinase, endochitinase and<em>β</em>-1,3 glucanase activity. Universally primed PCR (UP-PCR) and inter-simple sequence repeat (ISSR) techniques were used to examine the genetic variability among isolates, which resulted in 127 bands for the total number of isolates. These results were subjected to numerical analysis revealing 20 haplotypes grouped in five clusters. The ability of<em>Trichoderma</em>isolates to antogonize soil-borne fungal plant pathogens using a dual culture assay was done against five fungal species:<em>Alternaria</em>sp.,<em>Bipolaris sorokiniana, Fusarium graminearum</em>,<em>F. solani</em>, and<em>Pyricularia oryzae.</em>The highest inhibition values (85% RI) were obtained against<em>B. sorokiniana</em>and<em>P. oryzae.</em>Three isolates of<em>T. harzianum</em>named as FCCT2, FCCT3 and FCCT9 were capable of causing a high growth inhibition on four of the fungal species assayed, which was in agreement with their higher extracellular hydrolytic activity. Our results suggest that these isolates have the potential to be effective agents for biocontrol of cereal and tomato fungal pathogens. |
description |
Monoconidial cultures of 33 isolates of<em>Trichoderma</em>from Buenos Aires Province, Argentina were characterized on the basis of twenty eight morphological, physiological and biochemical features. All of them were screened for proteinase, endochitinase and<em>β</em>-1,3 glucanase activity. Universally primed PCR (UP-PCR) and inter-simple sequence repeat (ISSR) techniques were used to examine the genetic variability among isolates, which resulted in 127 bands for the total number of isolates. These results were subjected to numerical analysis revealing 20 haplotypes grouped in five clusters. The ability of<em>Trichoderma</em>isolates to antogonize soil-borne fungal plant pathogens using a dual culture assay was done against five fungal species:<em>Alternaria</em>sp.,<em>Bipolaris sorokiniana, Fusarium graminearum</em>,<em>F. solani</em>, and<em>Pyricularia oryzae.</em>The highest inhibition values (85% RI) were obtained against<em>B. sorokiniana</em>and<em>P. oryzae.</em>Three isolates of<em>T. harzianum</em>named as FCCT2, FCCT3 and FCCT9 were capable of causing a high growth inhibition on four of the fungal species assayed, which was in agreement with their higher extracellular hydrolytic activity. Our results suggest that these isolates have the potential to be effective agents for biocontrol of cereal and tomato fungal pathogens. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/submittedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
submittedVersion |
dc.identifier.none.fl_str_mv |
https://digital.cic.gba.gob.ar/handle/11746/5624 |
url |
https://digital.cic.gba.gob.ar/handle/11746/5624 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-nd/4.0/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by-nc-nd/4.0/ |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:CIC Digital (CICBA) instname:Comisión de Investigaciones Científicas de la Provincia de Buenos Aires instacron:CICBA |
reponame_str |
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CIC Digital (CICBA) |
instname_str |
Comisión de Investigaciones Científicas de la Provincia de Buenos Aires |
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CICBA |
institution |
CICBA |
repository.name.fl_str_mv |
CIC Digital (CICBA) - Comisión de Investigaciones Científicas de la Provincia de Buenos Aires |
repository.mail.fl_str_mv |
marisa.degiusti@sedici.unlp.edu.ar |
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13.070432 |